Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	86,271	C→T	G214G (GGC→GGT)	ilvI →	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	86,271	0	C	T	95.8%	138.0 / ‑1.7	48	G214G (GGC→GGT)	ilvI	acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (20/26);  total (20/28)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.84e-01

ACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAA  >  NC_000913/86032‑86498                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    aCGGAAGACATTCCGCAGGTGTTGAAAAAGGATTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATAATCTTAATCCGGCGAACAAAGTACCCTAGGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTGCCGGACTTAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAAGCGGTTGTCTACGTAGGTGGTGGGGCAAt                                                                                                                                                                                                                          <  1:424227/251‑1 (MQ=255)  cGGAAGACATTCCTCAGGTGCTGAAAAAGGCTTTCTTGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTGCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATc                                                                                                                                                                                                                         <  1:437989/251‑1 (MQ=255)                cAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGcc                                                                                                                                                                                                            >  1:407324/1‑250 (MQ=255)                     gCTGAAAAAGGCTTTCGGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGAGTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTGCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATcag                                                                                                                                                                                                       <  2:166575/250‑1 (MQ=255)                       tGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATccgc                                                                                                                                                                                                      >  1:451065/1‑246 (MQ=255)                        gAAAAAGGTGTTCTGGCTGGCGGCAAGTGGTCGCCCTGGACCAGTAGTTGTTGATTTACCTAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCGGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCTGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATcatcag                                                                                                                                                                                                    <  2:346294/250‑1 (MQ=255)                         aaaaaGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCATATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCACCAGCAGtt                                                                                                                                                                                                  >  1:315896/1‑251 (MQ=255)                           aaaGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTga                                                                                                                                                                                                <  1:112558/251‑1 (MQ=255)                            aaGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTgaa                                                                                                                                                                                               <  1:457370/251‑1 (MQ=255)                            aaGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGGAGGTGGGGGGGCAACCACGGCGGGcgtccagaaggagatgg                                                                                                                                                                                                >  2:247782/1‑234 (MQ=255)                              ggCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTgaaag                                                                                                                                                                                             <  1:326005/251‑1 (MQ=255)                                 tttCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTgaaagaa                                                                                                                                                                                           >  1:413124/1‑250 (MQ=255)                                       gctggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGTTATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCGTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAAtggtgg                                                                                                                                                                                    <  1:302556/251‑1 (MQ=255)                                          ggcggcAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAg                                                                                                                                                                                  <  1:323492/250‑1 (MQ=255)                                              gcAAGTGGTCGCACAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGGTGCCCTCCGCAATTgaaagaaaatgtggggggggg                                                                                                                                                                             >  2:210925/1‑238 (MQ=255)                                                     gTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTCGCCGGCGTCGGTCAGTATGCGTTCTTACAATCCCCCTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAGACGCTGGTAGCGGCAAAAAAACCGGATGTCTACGTAGGTGGTGGGGCATTCACGACGGGCGGCCATACGCAGTTGAAAGAAATGGAGAAGGCGTTGAATCtt                                                                                                                                                                      >  1:189524/1‑250 (MQ=255)                                                              gACCAGTAGTCTTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGGCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCgttgtt                                                                                                                                                             <  2:412463/251‑1 (MQ=255)                                                                     agtCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCAGCCATCAGCAGTTGAAAGAAATGGGGGGGGGGTTGAATATGCCCGTTGGTTCCTCAt                                                                                                                                                      >  1:78712/1‑251 (MQ=255)                                                                       tCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGGATCTGACCGTTGTTTCCTCAttt                                                                                                                                                    >  1:205669/1‑250 (MQ=255)                                                                           tGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATg                                                                                                                                                 <  2:145484/250‑1 (MQ=255)                                                                              tttACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGGAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATggggg                                                                                                                                             >  1:394342/1‑250 (MQ=255)                                                                                     aaaGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGc                                                                                                                                      <  1:357232/251‑1 (MQ=255)                                                                                     aaaGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGAGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGc                                                                                                                                      <  1:350501/251‑1 (MQ=255)                                                                                       aGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCAt                                                                                                                                                                                                          <  1:238082/181‑1 (MQ=255)                                                                                          tatTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTc                                                                                                                                 >  1:7790/1‑251 (MQ=255)                                                                                                tAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGGGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCCTCACCAGTTGAAAAACATGTTTGAGGCGTTGAGTATGCCCGGTGTTTCCTTATTGATGGGGCGCGGGGCGTTTTCGGCa                                                                                                                            >  2:39807/1‑250 (MQ=255)                                                                                                 aaTCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAAc                                                                                                                          <  1:464324/251‑1 (MQ=255)                                                                                                 aaTCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGGCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGTCTGGGGGCGTTTCCGGCAAc                                                                                                                          <  2:293332/251‑1 (MQ=255)                                                                                                  aTCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGGTGTTTCCTCATTGCTGGGGCTGGGGGCGTTTCCGGCAACg                                                                                                                         >  1:245910/1‑251 (MQ=255)                                                                                                           aCAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAg                                                                                                                <  2:7790/251‑1 (MQ=255)                                                                                                                  aCCCTAGGTCTGGCCGGAGGTGGACAGTATGCGTACTTACAATCCCACCATTACCGGACATAAAGGGCAAAGTAAGCGTGCTCTGCAAACGCTGGGGGCGGCAAAAAAACCGGTTGGCTCCGTAGGTGGGGGGGCAAGCACGGCGGGCTGCCCTCAGCAGTTGTAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTgg                                                                                                         <  2:104559/251‑1 (MQ=255)                                                                                                                                 ggAGTCGGACCGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCAGCCATCAGCAGTTGAAAGAAATGGGGGAGGAGTTGAATCTGCCCGGTGTTTCCTCATTTGTGGGGCTGGGGGCGGTTTCGGCCACGCATCGTACGGCACTGGGGATGCTggggagac                                                                                          >  2:413800/1‑246 (MQ=255)                                                                                                                                           aGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCCGCCAACAGCCGTTGAAAAAAATGGGGGAGGGGTTGGAGCTGCCCGTTGTTTCCTCATTGGTGGGGCTGGGGGAGCTTCCGGCAACGCATCGTCAGGACCTGGGGATGTTGGGGATGCACGGTTCCtc                                                                                >  2:330193/1‑250 (MQ=255)                                                                                                                                            gTATGCGTTCTTACAATCCCACTACTACCGGACATAAAAGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTTGTGGGGCAATCACTGCGGTCTGCCATCAGCAGTTGAAAGAAATGGTGTAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGTGGCTTGGTACGTTTCCGGCAACGCATCGTCAGGCACTGGCCATTCTGTTAATACACTGTACCTAc                                                                               <  2:208449/251‑1 (MQ=255)                                                                                                                                              aTGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTTCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACgg                                                                             >  1:315014/1‑250 (MQ=255)                                                                                                                                              aTGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACg                                                                              <  2:83296/250‑1 (MQ=255)                                                                                                                                              aTGCGTTCTTACAATCACACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGGGGCGTTGAATCTGCCCGTTGTTTTCTCATTGATGGGGGCGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGTATGCACGGGACCTACg                                                                              >  1:377529/1‑250 (MQ=255)                                                                                                                                                gCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAg                                                                           <  1:247782/251‑1 (MQ=255)                                                                                                                                                   ttCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCCGCAGTTGAAAGAAATGGTGGAGGCGTTGAATATGCCCGTTGTTTCCTCATTTATGGGGCTGGGGGCGGTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGTATGCACGGTACCTACGaatcc                                                                         >  1:378430/1‑247 (MQ=255)                                                                                                                                                    tattACAATCCCACGACTACCGGACATAAAGGGCAAATTAAGCGGGCTCTGCAAACGGTGGTGGCGGCAAAAAAACCGGTTGTCGTCGTAGGTGGTGGGGCAATCCCGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCTTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCa                                                                        <  2:187362/248‑1 (MQ=255)                                                                                                                                                    tCTTACAATCCCTCTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCa                                                                        <  1:271638/250‑1 (MQ=255)                                                                                                                                                                   actacCGGACAGAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCAt                                                          <  1:307308/249‑1 (MQ=255)                                                                                                                                                                           aCATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCCACGCATCGGCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGAtg                                                >  1:297646/1‑251 (MQ=255)                                                                                                                                                                                aaGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCCGCAGTTGAAAGAAATTGGGGAGGGGTTGAATCTGCCCGTTTGTTCCTCATTGTTGGGGCTGGGGGGGTTTTCGGCCACGCCTCGTCCGGGACAGGGCATGCTGGGGATGCACGGGACCTACGAAACCAATATGTCGATGCATaatcaggtgttgctt                                           >  2:93251/1‑238 (MQ=255)                                                                                                                                                                                       atgtAAGCGCGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCAGAACGCGGATGTGATTTTCGCCg                                    <  2:377529/248‑1 (MQ=255)                                                                                                                                                                                                                  ggCGGCAAAAAAACCGGTTGTCTACGGAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAAGCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAacg          <  2:315014/249‑1 (MQ=255)                                                                                                                                                                                                                         aaaaaaCCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACaa  <  1:357071/251‑1 (MQ=255)                                                                                                                                                                                                                                    tGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTAc                           <  1:191164/215‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    ACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTGCTCATTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAA  >  NC_000913/86032‑86498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑