Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 66,403 C→T V50I (GTC→ATC)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091366,4030CT95.9% 138.3 / ‑2.2 49V50I (GTC→ATC) araDL‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (21/26);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

ATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGA  >  NC_000913/66156‑66647
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
aTTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGGATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGGGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGCTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCt                                                                                                                                                                                                                                                   >  1:157203/1‑251 (MQ=255)
            cAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGcc                                                                                                                                                                                                                                       <  1:124492/251‑1 (MQ=255)
                       gCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGa                                                                                                                                                                                                                            <  1:413474/251‑1 (MQ=255)
                       gCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGa                                                                                                                                                                                                                            <  1:198964/251‑1 (MQ=255)
                             gAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATTGACGCCGGAAGGTTTGATcaca                                                                                                                                                                                                                       >  1:133066/1‑250 (MQ=255)
                              aaaTAGTCGGCTTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACaaa                                                                                                                                                                                                                     <  1:379824/251‑1 (MQ=255)
                                         gtgggtggTGCCGGTTGTTGGATGCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGTCGCGAGGGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCCCCTTCAACCACTTCACCGGTTTCTATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCt                                                                                                                                                                                                          <  1:423751/251‑1 (MQ=255)
                                                 tgCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGAAGCCGCGCTCGCGATc                                                                                                                                                                                                   >  1:293713/1‑250 (MQ=255)
                                                            gaaaccgcttggcccGCTTGCGCCCGGATAGTGGCGTGGCGTGAGTGTGTGTGCACAATGCCGCCAATGGACGGTAATGCCTGATAGAGCAGTCGGTGAGGTGGCGTATCGGAGGAGGGCTTTTTCGCAGTTTCAACCACTTCACCGTGTTCGATGCTAACCACGTCCATAGCGTCATCGCTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGAc                                                                                                                                                                                       <  1:427298/239‑1 (MQ=255)
                                                                    ctggcccGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGcccc                                                                                                                                                                               >  2:160563/5‑251 (MQ=255)
                                                                           gCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAg                                                                                                                                                                        <  1:41979/251‑1 (MQ=255)
                                                                                cgcCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGa                                                                                                                                                                   <  1:160563/251‑1 (MQ=255)
                                                                                    cAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATAGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGCTCAACGGCGCTGACGTTTCCCCATGTGAGCGTGAcc                                                                                                                                                                 >  1:88820/1‑249 (MQ=255)
                                                                                       atagtggcgtggcGCGAGTGTTGGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGtt                                                                                                                                                            <  1:438626/248‑1 (MQ=255)
                                                                                               gtggcgCGAGGGGGTGTGCACAAGGCCGACAATGGACGGGAATGACTGATAGAGCAGTCGGTGAGTTGGCGTATAGGAGGAGGGCTTTTGCGCACCTTCAACCACTTCACCGGTTGCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGTCCCATGTGAGCGTGACCAGGTTATGTTTTg                                                                                                                                                    <  1:185370/251‑1 (MQ=255)
                                                                                                 ggcgCGATTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTgg                                                                                                                                                   <  1:114107/250‑1 (MQ=255)
                                                                                                   cgcgAGTGTGTGTGCCCAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTTAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAg                                                                                                                                                <  1:2956/251‑1 (MQ=255)
                                                                                                   cgagAGTGTGTGTGCACAATGCCGCCAATGGAAGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAGAGACGCAGCGCTCGCGTTCAACGGCGCTGACGTTGCCCCATGGGAGCGTGACAAGGTTATGTTTTGGc                                                                                                                                                  >  2:180700/4‑249 (MQ=255)
                                                                                                          ttttgtgctcactGCCGCCAATGGTCGGGAACGCCTGATAATGCAGGCGGTTATTTGGCGTATCGGGGGAGGGCTTTGTCGCACCTTCAACCTCTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATAGACGCCGGAAGGGTTGATCACAAAGACGCCGCGCTCGCGATCAACTGCGCTGCCGTTGCCCCATGTGAGCGTGACCAGGTTATTTTTCGGCAGCGCCAg                                                                                                                                          <  2:251870/238‑1 (MQ=255)
                                                                                                                 cacaATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGcct                                                                                                                                  >  1:255199/1‑249 (MQ=255)
                                                                                                                  acaATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGcctc                                                                                                                                 >  1:110375/1‑248 (MQ=255)
                                                                                                                       gccgccAATGGACGGGAATGCCTGATAGAGCAGTAGGTGAGTTTGCGGATCGGAGGAGGGCGTTTTCGCACCTTCAACCACTTCACCGGGTGCGAGGCTAACCGCGACCATATCGTCATCGGTCATGATGCTGTAAGCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACTAGGTTATGTTTTGGCAGCGCCATGTGGGCTTCTAAtt                                                                                                                            <  2:426025/251‑2 (MQ=255)
                                                                                                                           ccAAAGGACGGGAATGCATGATTGAGCAGTCTGTGAGGTGGCGTATCGGGTGTGGGCGTTTTCGCACCTTCAACCACGTCACCGGGTTCGATGCTAACCACGACCATCGCGTCAGCGGTCATGATTCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGTGCCGACGTTGCCCCATGTTAGCGTGACCAGGTTATGTTTTGGCAGCTCCAGGTTGGCCTCTAATatct                                                                                                                         <  2:235404/250‑4 (MQ=255)
                                                                                                                             aaTGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCGCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGc                                                                                                                      >  1:109344/1‑251 (MQ=255)
                                                                                                                               tGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGt                                                                                                                    >  1:463043/1‑251 (MQ=255)
                                                                                                                                  acgggAATGCCTGATAGAGCCGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGCCCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTCATACCTGGCGTTTg                                                                                                                 >  2:445770/3‑251 (MQ=255)
                                                                                                                                  acgggAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTg                                                                                                                 <  2:310626/249‑1 (MQ=255)
                                                                                                                                    gggAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTg                                                                                                                 >  1:282904/1‑249 (MQ=255)
                                                                                                                                              gATAGAGCAGTCGGTGAGTTGGCGTATCGGGGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGGCGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCGTGTGAGCGTGACCAGGTTAGGTTGTGGCAGCGCCAGGTTGGCTTCTAATACCTGGTGTTTGAGATCTTCTa                                                                                                       <  1:407734/249‑1 (MQ=255)
                                                                                                                                              gATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCATGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTaa                                                                                                      <  2:418595/250‑1 (MQ=255)
                                                                                                                                                 agagCAGTCGGTGAGTTGGCGTATCGGAGTAGGGCTCTTTCGCACCTTCAACCACTTCCCCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAAGCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGCCGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCATCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACAt                                                                                                   <  2:129173/250‑1 (MQ=255)
                                                                                                                                                   agcagtCGGTGAGGTGGCGTATCGGAGGCGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGACAGCGGTCATGATGCTGTACTCGACGCCGGAAGGTTTGATAACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTGATAACTGACGTTTGAGATCTTCTAAAATGtt                                                                                                >  2:399782/1‑251 (MQ=255)
                                                                                                                                                          ggTGAGTTGGCGTAGCGGAGGAGTGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCt                                                                                         <  2:199514/251‑1 (MQ=255)
                                                                                                                                                              aGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAATGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATCCCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGt                                                                                     >  1:244836/1‑251 (MQ=255)
                                                                                                                                                                      tatCGGAGGCGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTACCCACGACAATATCGTCAGCGGTCATGCTGCTGTAATCGTCGCAGTAAGGTTTGATCACAAAGTAGACGCGCTCGCGATCAACGGCACTGACGTTGCCCCATGCGAGCGCGACCAGGTTATATTTTGGCAGCGCCAGGTTGACCGCTAATACCTTGGTTTTGGGATCTTCTACAATGTTAACTACTGCGTGACGGagt                                                                             >  2:334826/3‑249 (MQ=255)
                                                                                                                                                                           gaggagGTCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATAGCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTGGCCCCATGTGAGCGTGACCAGTTTATGTTTTGGCATCGCCAGGTTGGCCTCTAATGCCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCtt                                                                        <  1:440028/251‑1 (MQ=255)
                                                                                                                                                                              gaggGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCCCGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGCCGTTGCCCCATGTGAGCGAGACCAGGTTATGTCTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTATCTCCTTCTTGCCGGTTGCGCTTTGc                                                                     >  2:282039/1‑251 (MQ=255)
                                                                                                                                                                               aggGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCt                                                                    <  1:400719/251‑1 (MQ=255)
                                                                                                                                                                                        tcgcaCCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATAGACGCCGGAAGGATTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGcc                                                           >  1:443901/5‑251 (MQ=255)
                                                                                                                                                                                                    ccACTTCACCGGTTTCGATGCTAACCCCGACCATATCGGCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGTCTACAAAATCGCa                                               <  2:109344/251‑1 (MQ=255)
                                                                                                                                                                                                    acACTTCCCCGGTTTCGATGCTAACAACGACCATATCGTCAGCGGTCATGATGCTGGAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGGGACCGGGTTATGTTTTGGCAGCTCCAGGTTGGCCTCTAATACCTGGCGTTTGCGATCTCCTAACATGTTGACTCCTTCGTGCCGGATGCGCATTGCTTATCCGGCCTACAAATTCGc                                                >  2:328971/2‑250 (MQ=255)
                                                                                                                                                                                                         taaCCGGTTGCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGCGCGTGACCCGGTAATGTTTTGGCAGCGCCAGGCTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAAACTGTTGACTCCTTCGTCCCGGATGCGCCTTGCTTATCCGGCCTACATCATCGCAGCgtg                                          >  2:237211/3‑251 (MQ=255)
                                                                                                                                                                                                                    gATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAACTCGCAGCGTGTAGGCCTgggg                               >  2:119512/1‑248 (MQ=255)
                                                                                                                                                                                                                    gATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTg                                  <  1:119512/248‑1 (MQ=255)
                                                                                                                                                                                                                           aCCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCATCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGAcgcg                        <  1:90375/251‑1 (MQ=255)
                                                                                                                                                                                                                               cGACCATATCGGCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACgcgctag                    <  2:282904/251‑4 (MQ=255)
                                                                                                                                                                                                                                 aCCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAATGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCg                  <  2:385792/251‑1 (MQ=255)
                                                                                                                                                                                                                                         gTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGGTAAGACGCGCCAGCGTaaagttg          >  2:308699‑M1/1‑244 (MQ=255)
                                                                                                                                                                                                                                                  cATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGAGCTCGCGATCAACGGCGCTGACGATGCCCCATGTGAGCGAGACCAGGATATGTTTTGGCAGCGCCAGGTTGGCCTCTAATAACTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTAAAAAATCGACGCGGTTAGGCCTGATAAAACGCGCCAGCGTaaagtgggcactgtg  >  1:324942‑M1/1‑235 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
ATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGA  >  NC_000913/66156‑66647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑