Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,652,3310TC94.4% 56.5 / ‑5.7 18intergenic (+2346/+397)ydfD/ynfPlysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (5/12);  total (6/12)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAAC  >  NC_000913/1652089‑1652561
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       
ggTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTCagagag                                                                                                                                                                                                                                 <  1:302923/251‑1 (MQ=255)
              tataCTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATCGGGTGCTTAATCTTATTTAACCAGCAAACGACGCTCTCCGGAGGCAGATGACATTAAAGAGAATGAGCAAAGAGCATAACAATGGTGTTTCACT‑CAGAGAGGATGGgagaac                                                                                                                                                                                                                      >  2:150794/1‑248 (MQ=255)
                                                                       aaaaaTGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGAATTCATCTTATTAAGCCAGCAATAGACGCTCTCCGGAGTCAGAAGACATTAACGAGACTGAGCAAAGGGCATATCATTGATGTT‑CACCTCAGAAAGTAAGGCAGAACAAAAAAAAAAAAATAAACCTTTGTTTTTCAACCTGAAGTGTCAACGTAG‑GTAAAAAAtt                                                                                                                                                          >  1:312031/1‑250 (MQ=255)
                                                                             ggAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGa                                                                                                                                                   <  1:150794/251‑1 (MQ=255)
                                                                              gAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCt                                                                                                                                                                                                  <  1:13608/204‑1 (MQ=255)
                                                                              gAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGAc                                                                                                                                                  <  1:239153/251‑1 (MQ=255)
                                                                                            aGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTTCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGa                                                                                                                                    <  2:416732/251‑1 (MQ=255)
                                                                                                               aCCGTTTCACGGTACCAAAAACACAGGCTGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATTACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTgg                                                                                                                 <  2:373626/251‑1 (MQ=255)
                                                                                                                      cACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAg                                                                                                          <  2:268986/251‑1 (MQ=255)
                                                                                                                      cACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTTTTAAGACAACAATAGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATACACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGAAAAAAATTATGGTGAACATTTTACCGTTAACTCAATAAGGGAGATGTGGGACGc                                                                                                            >  1:294785/1‑249 (MQ=255)
                                                                                                                                     acaGGCGGGAACCAAACGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCg                                                                                           <  2:367966/251‑1 (MQ=255)
                                                                                                                                                        gTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATc                                                                        >  1:247036/1‑251 (MQ=255)
                                                                                                                                                                      gaagacaggcaTCGACGCTCTCCGGAGTCAGATGACATTAAAGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCgaga                                                           <  2:312449/240‑1 (MQ=255)
                                                                                                                                                                                      gCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgctg                                          <  1:387796/251‑1 (MQ=255)
                                                                                                                                                                                      cctctCCGTAGTCAGAGTACATTAACGAGACTGAGCAAAGAGCATATTATTGATGTT‑CATCTCAGAGAGTATTGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGACAGCG‑AAAGTAAAAAATAATGGTGACCATTTTACCGTTGACATAATAAGGCAGATGTGGGACGCAGCGATAAAACGTTCCGGACGCCGCCATCGAAAATCAGATCAGTCGAGACAtttgtattcctgctt                                          <  2:238403/250‑15 (MQ=255)
                                                                                                                                                                                                                              agCATATCATTGATGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAAc  >  2:75300/1‑251 (MQ=255)
                                                                                                                                                                                                                                           tGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATaaa                                                                                                   >  1:25903/1‑141 (MQ=255)
                                                                                                                                                                                                                                           tGTT‑CACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATaaa                                                                                                   <  2:25903/141‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       
GGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTT‑CACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCG‑AGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAAC  >  NC_000913/1652089‑1652561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑