Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,215,3470TA88.9% 43.3 / 0.2 18intergenic (+138/‑131)metA/aceBhomoserine O‑succinyltransferase/malate synthase A
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (8/8);  total (9/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.25e-01

CGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATT  >  NC_000913/4215103‑4215572
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 
gaagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTTGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTGCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTataatta                                                                                                                                                                                                                             <  2:55988/249‑1 (MQ=255)
    cTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAAt                                                                                                                                                                                                                         >  1:318885/1‑251 (MQ=255)
                                aCCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGCAATTGTTTTTGATTTTGCATTTTa                                                                                                                                                                                              >  2:218312/1‑250 (MQ=255)
                                    aCTGGCTCAACTATTCCGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGa                                                                                                                                                                                         <  2:375372/251‑1 (MQ=255)
                                                 ttACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGtt                                                                                                                                                                             <  1:338950/250‑1 (MQ=255)
                                                         aCCAGATCACGCCATACGATCTACGTCTCATTAATCCAACGCTGGATTAATCTTATGTGATAGTCGATCGTTAAGCGATTCAGCCCCTTTCCTCAGGCACCTTCGTGTGCCTTGTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTTCATGTTAAATGAGTAGTCTTAGTTGTGCTGaa                                                                                                                                                                     <  1:31238/250‑1 (MQ=255)
                                                         aCCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTg                                                                                                                                                                       <  1:195436/248‑1 (MQ=255)
                                                                cACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGAttt                                                                                                                                                                                                       >  2:126355/1‑209 (MQ=255)
                                                                cACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTAGCAAGTATTTTTAATTAAAAGGGAAATTGTTTTTGAttt                                                                                                                                                                                                       <  1:126355/209‑1 (MQ=255)
                                                                                                   tGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATACTTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGACCGAAAAGAGCACAACGATCCTTCGTTAACGGTGGGGAAGtttt                                                                                                                          >  1:51570/1‑251 (MQ=255)
                                                                                                              tctGTTATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAAGTGAATAAATTTTATTCATATTGTTAACAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGATCGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATg                                                                                                                <  2:361283/250‑1 (MQ=255)
                                                                                                               ctGTGATAGTAGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTAGGGTGCCTTTTTTATTTCCGACACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAGTTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGAc                                                                                                              >  2:214318/1‑251 (MQ=255)
                                                                                                                           aTCGTTAAGCGATTCAGCACCTTCCCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTCTTCATATTGTTATCAACAAGTTCTCAGGTATTTATAATTAAAATGGAAATTGTTTTTGTTTTTGCATTTTCAATGAGTAGACTTAATTGTGCTGAACGAAAACAGCCCAACGATCCTTCGTTCACAGTGGGGGAGTTTCCGGATCCATGACGAGGAGCTGCAc                                                                                                  >  1:453102/1‑251 (MQ=255)
                                                                                                                                                                            ttATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGcc                                                  <  1:345327/250‑1 (MQ=255)
                                                                                                                                                                            ttATTGCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGGATTTATAATTAAAATGGATATTGTTTTTGATTTTGCATTTTAAATGATTAGTCTTAGTTGTGCTGAACGAAAAGATCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGcc                                                  <  1:242347/250‑1 (MQ=255)
                                                                                                                                                                                     aaaCGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCg                                          >  1:49218/1‑249 (MQ=255)
                                                                                                                                                                                                                    tCATATTGTTATCAACAAGTTATCAAGAATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCgg         >  1:469750/1‑251 (MQ=255)
                                                                                                                                                                                                                          tGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAGTTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTACTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCACCAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGCAGCAAATTCTTACTGCCGAAGCGGTAGAAt   >  2:356364/1‑251 (MQ=255)
                                                                                                                                                                                                                            ttATCAACAAGTTATAAAGTATTTATAATTAAAATGGAAATTTTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCAAAACGATCCTTAGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCGTACTGCCGAAGCGGTAGAAtt  <  2:47974/250‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                 
CGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATT  >  NC_000913/4215103‑4215572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑