| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,825,328 | G→A | S83S (TCC→TCT) | mltB ← | membrane‑bound lytic murein transglycosylase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,825,328 | 0 | G | A | 88.7% | 122.7 / 4.5 | 56 | S83S (TCC→TCT) | mltB | membrane‑bound lytic murein transglycosylase B |
| Reads supporting (aligned to +/- strand): ref base G (2/4); new base A (20/27); total (25/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 | |||||||||||
CCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGGGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAA > NC_000913/2825082‑2825573 | ccAGCGGGGTTCAACGCCGATAATCCCGACGATAATGTCCGGCGGTACTCCATCCACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTGGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGCCCTGATGTCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTgagaga < 1:283278/251‑1 (MQ=255) tccaacccctaTAACCCCGACGATAATTACCGGCGGTACTCCATACCCCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTGCGGTCCTTATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGGGATAGAATTTcc < 2:218032/240‑1 (MQ=255) gcAACGCCGATAATCCCGAAGATAATTTCCGGTGGTACGCCAGACACCTGACACGCGCGATTCAACGCATTTTCATACTGATTACAGAAAACCACACCGTTCTGCATGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGTCGTTAGGACCTGATGGCGGTTTCACCGATGTTGTTGGTGCCTGGTTATCCATCAGCCGCAGTCCCGAATCC‑AGACGGTTCGCCTGAGAGAGAACTTCCt < 2:156433/249‑1 (MQ=255) ccGATACTCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACTCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCTCGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAActg < 2:143272/251‑1 (MQ=255) ccGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGCGAGAGAATTTCCTGCAActg > 1:87136/1‑251 (MQ=255) gATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAATCCACACCGTTCTGCACGTTTTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAActgct < 1:231227/251‑1 (MQ=255) caccGAAGATAATTCCCCGGGGTACTCCATCCACCTGCCCCGCGCGATTCAACTCATCTTCATGCTGATTCCAGAAATCCGCGCCGTTCTGCACGGGGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGTCCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGTTTATCCATCAGCCGCAGTACCGAATGC‑AGGCTCTTCTCCTGAGAGAGAATGTCCTGCAACTGCTGACGa < 2:427848/249‑1 (MQ=255) aTAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAAcc < 1:128901/251‑1 (MQ=255) tAATTTCCGGCGGTACTCCATACCCCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGGGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑CGACGCTTCGCCTGAGGGGGACTTTCCTGCAACTGCGGACGATCGAAAc > 2:342237/1‑249 (MQ=255) tAATTTCCGGCGGTACTCCATAAACCTGCCACGCGCGATTCCACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAACTTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGGTTTCCATCCGCAGCCGGACCGCATCCCAG‑CGCTTCGCCTGAGAGAGAATTTCCTCCCACTACTGACgcgagcacccc > 2:190406/1‑241 (MQ=255) aaTTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCgg > 1:113982/1‑250 (MQ=255) ccGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGtgttt < 1:52639/250‑1 (MQ=255) gcggTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTc < 1:110808/251‑1 (MQ=255) cggTACTCCGTACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCAATCAGCCGCAGTACCGAATCC‑AAACGCTTCGCCTGCGTGAGAATTTCCTTCAACTGCTGACGATCGAAAACGTGTTTGTTc > 1:342121/1‑250 (MQ=255) ccATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGAAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGAAACTGCTGAAGATCGAAACCGAGTTTGTTCACCAtttt > 1:223846/1‑251 (MQ=255) cATACCCCTGTCACGCGCGATTCAACGCATCTTCATACGGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTGTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTg < 1:22653/251‑1 (MQ=255) cATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGCTGGGGATGGAGCCTGGTTATCCATCAACCGCAGTACCGAATCC‑AGACGCTTCGCCTGCGAGAGAATTTCCTACACCTTCTGACGAACCAAACCGGGTTTGTTCACCCttttt > 2:328583/1‑250 (MQ=255) ccACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAAct > 1:399023/1‑251 (MQ=255) cgcgcgATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGGTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAActgct > 1:234339/1‑251 (MQ=255) cgATTAAACGCAGCTTCATACTGATTCCAGAAAACCACACCGTTCTGCCCGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGATCTGCTGGGc < 2:79635/251‑1 (MQ=255) gattctccATACTGATTACAAAAACCCACACCGTGCTGCTCGCTGTCCGGCGTAATAAATTTTTTGCCATAACGGAGCCATGCGACGCTCGGAACTGATGGCGGTTTCCCCGATGCGGATGGTGCCTGGTTATTCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCAGCAACTGCTGACGATTGAATACGTGTTTGTATACCATTTTGTCGATGAACTGCTGGGCATTCGGGtt < 2:282081/244‑1 (MQ=255) gCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATACCGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGACTGAGCGAGAATTTCCTGCAACTGCTGCCGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGtt > 1:424203/1‑251 (MQ=255) tCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGTCTCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGttatt < 1:310109/248‑1 (MQ=255) aCCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGGGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCAATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTg < 1:47733/251‑1 (MQ=255) acacCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGGGCCTGGTTATCCATCAGCCGCAGTACCGGATCC‑AGACGCTTCGCCTGGGAGAGAATTTCCTGCAACTGCTGACGAACGAAACCGGGTTTGTTCACCATTTTGGCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCAt > 2:187245/1‑251 (MQ=255) acacCGTTCTGCACGCTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAAGCC‑AGACGCTTCGCCTGGGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCAt < 2:98542/251‑1 (MQ=255) ccGTTCTGCATGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATcac > 1:39338/1‑251 (MQ=255) ttCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGGGTTTGTTCCCCATTTTGTCGATGAACTGCTGGGCATTCGGGTTTTTAGCGAAATCACACCCCCTCTGCATccaatt > 2:249953/1‑246 (MQ=255) cGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGACTGGTTATACATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGAACGAAACCGTGTTTGTTCACCCTTTTGTCGATGGACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCAAACTGCATCACATTGtgctgc > 1:93146/1‑251 (MQ=255) ttGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCAACGATGTGGGTGGGGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCAACATTTTGGCGATGAACTGCTGGGCATTCGGGGTATTAGCGAACTCGCCGCCCATCTGCACCACATTGTGCTGCgg > 2:54917/1‑251 (MQ=255) gTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGATTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCt > 1:317527/1‑251 (MQ=255) gTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGAGGTTGGTGCCTGGTTATCCATAAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGGGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGACCTGCTGGGCATTAGGGTTATTATCGAAATCGCCGCCCATCTGCATCCCCTTGTGCTGCGGCCCAAGc > 2:156981/1‑249 (MQ=255) aaaTTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAg < 1:156912/250‑1 (MQ=255) tttttGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAgccgc < 1:46675/250‑1 (MQ=255) tttGCGATAACGGAGCCATGCGCCGTTCGAACCTGATGGCGGTTTCCCCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑CGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCACCGCCCATCTGCATCCCATTGTGCTGCGGCTCAAGCAGGAAgccgcc > 1:375640/1‑249 (MQ=255) cGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCCCCATTTTGTCGATGAACTGCTGGGCCTTCGGGTTATTAGCGAAATAGCCGCCCATCTGCCTCACATTGTTCTGCGGCTCAAGCAGGAAACCGCCCGACgg > 2:244530/1‑251 (MQ=255) gACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGGGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCa < 1:187245/251‑1 (MQ=255) gACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTTACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGtctc > 1:135177/1‑250 (MQ=255) gATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCACCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAgg < 1:145655/251‑1 (MQ=255) ttCACCGATGTGGTTGGTGCCTGGTTATCCCTCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGGTCCGGTGGGCGTATCAGTATCAGTAGGTTTTGGCtt > 2:369139/1‑251 (MQ=255) cACCGATGTTGTTGGTGCCTGGGTATCCATCATCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGATAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTtgc < 2:172968/251‑1 (MQ=255) aCCGATGTGGTTGGTTCCTGGTTAGCCAGCAGCCGCATTACCGAATCC‑CGACGCTGCGCCTGATAGATAATTTCCTTCAACTGCGGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGATCTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTtgct < 2:183857/251‑1 (MQ=255) gATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTgctgct < 1:369975/251‑1 (MQ=255) gtgacTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGgca < 2:399023/247‑1 (MQ=255) tATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAacac < 1:337512/250‑1 (MQ=255) ccATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTTGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACaaaa < 1:465380/251‑1 (MQ=255) ccATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACGTTGTGCTGCGGCTCAAGCAGGACGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGATACAGGCGGCCAGCAACACaaa > 2:298386/1‑250 (MQ=255) aGTACCGAATCC‑AGACGCTTCGCCTGAGGGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCa > 1:182167/1‑250 (MQ=255) gTACCGAATCC‑AGACGCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGGCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCTGCCAGACGGCGGTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAAt < 2:39338/251‑1 (MQ=255) aaccc‑AGACGCTTCGCCTGAGAGATAATTTCCTTCAACTGCTGACGATCGAAACCGTGTTTTGTCCCCATTTTGTCGATGAACTGCTGGGCATTCGGGTGATTATCGAAATCGCCGCCCATCTGCAGCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCTTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGTGGCAATCAACACAAAAAGGGGAAGTAATGTTACa < 2:416749/248‑1 (MQ=255) aaTCC‑CGACGCTTCGCCTTAGTGAGAATTTCCTGCAACTGCTGCTGATCGAAACCGTTGTTGTTCACCATTTTGTCGATGAACTGTTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCAGCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACa < 2:303585/251‑1 (MQ=255) aaGACGCTTCGGCTGAGAGAGAAATTCCTGCAACTGCTGACTAGCGAAACCGGGGTTGTTCACCATTTTGTCGATGATCTGCGGGGCATTTGGGTTATTAGCGAAATCGCCGCCCATCTGCATCTCATGGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCTTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGTAAGCAATGTTACATAac < 2:87136/250‑1 (MQ=255) aGACGCTTCGCCTGGGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTCTCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAacg > 2:188830/1‑251 (MQ=255) gCTTCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGc > 2:303486/1‑251 (MQ=255) tCGCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCt < 1:370348/249‑1 (MQ=255) cGCCTGAGAGAGTATTTCCTGCAACTGCGGACGATCGAAACCGTTGTTGTTCATCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATGGGGCTGCGGCTCAAGCAGGAAGCCTCTAGACGGCGTTCCGGTGGTCGTAGCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCACCACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGa < 2:375640/251‑1 (MQ=255) gCCTGAGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGaa < 1:342237/251‑1 (MQ=255) | CCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCCAGAAAACCACACCGTTCTGCACGTTGTCCGGCGTAATAAATTTTTTGCGATAACGGAGCCATGCGCCGTTCGGACCTGATGGCGGTTTCACCGATGTGGTTGGTGCCTGGTTATCCATCAGCCGCAGTACCGAATCC‑AGACGCTTCGCCTGGGAGAGAATTTCCTGCAACTGCTGACGATCGAAACCGTGTTTGTTCACCATTTTGTCGATGAACTGCTGGGCATTCGGGTTATTAGCGAAATCGCCGCCCATCTGCATCACATTGTGCTGCGGCTCAAGCAGGAAGCCGCCAGACGGCGTTCCGGTGGTCGTATCAGTCTCAGTAGGTTTTGGCTTGCTGCTACAGGCGGCAAGCAACACAAAAAGGGGAAGCAATGTTACATAACGACGCTTGAA > NC_000913/2825082‑2825573 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |