Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	809,217	2 bp→AG	coding (40‑41/1290 nt)	bioA ←	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	809,217	0	C	A	100.0%	116.9 / NA	39	W14M (TGG→CTG)	bioA	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/26);  total (13/26)
*	NC_000913	809,218	0	A	G	100.0%	136.7 / NA	39	W14M (TGG→CTG)	bioA	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/26);  total (13/26)

TTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTC  >  NC_000913/808976‑809455                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                              tgtGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTGCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATgg                                                                                                                                                                                                                                        <  2:285259/248‑1 (MQ=255)                   ggctggcGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGAt                                                                                                                                                                                                                     <  1:304815/251‑1 (MQ=255)                    gctggcGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATc                                                                                                                                                                                                                    <  2:237165/251‑1 (MQ=255)                      gtgcgatggggTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACGTCATCTCCGCATTAAGCTGCGGGTGTTTGGAGCCGGGGATCGCCGCCCACCAGGACGACAGACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCTCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGt                                                                                                                                                                                                                  <  2:256890/243‑1 (MQ=255)                                  gATACCGCCAAACATCACATGCGACATGGCAGCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATaa                                                                                                                                                                                                      <  1:196847/251‑1 (MQ=255)                                         ccAAACATCACATGCGACATGGCATCAATTTTCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACtt                                                                                                                                                                                               <  1:226546/251‑1 (MQ=255)                                               atcacatgagcattGGCATCAATTTGCGCCTCCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAAGCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAAc                                                                                                                                                                                         <  2:10079/238‑1 (MQ=255)                                                  acaTGCGACATGGCATCCATTTGCGACTTCCTCGCCGCATTAAGCTGCGGGTGATTGTAGCCGAGGATCGCCGCCCACCAGGCCAACATACCGTCAACAAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCAACGGATACACCGGCAGAGGGGAGGTCATGGCTGTGTATGGGCGCAGGCTATGGCGTTGGGCACAAGCATGATCGTCCGTTGTCAAAGTAGACTTGTAAACCa                                                                                                                                                                                       >  2:212579/1‑250 (MQ=255)                                                               cATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGAttt                                                                                                                                                                         <  1:193409/251‑1 (MQ=255)                                                                          gACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAg                                                                                                                                                              <  1:273781/251‑1 (MQ=255)                                                                               cATCGCCTCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATCCCGGCAACCAGGCGGCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCGCCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATGGAAAAGATTTAGGTTTACTAGTcga                                                                                                                                                          <  2:196496/250‑3 (MQ=255)                                                                                   gccgcATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCg                                                                                                                                                     <  2:127772/251‑1 (MQ=255)                                                                                        aTTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGCTGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAGGTTTGTCCGTTGTCATAATCGACTTGTAAACCAAATTTAAAAGATTTAGGATTACAAGGCTACACAGAAtt                                                                                                                                                 >  2:252877/1‑250 (MQ=255)                                                                                          tAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGACAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGGGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCACATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTaac                                                                                                                                              >  2:356333/1‑251 (MQ=255)                                                                                           aaGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTaaca                                                                                                                                             <  1:168279/251‑1 (MQ=255)                                                                                           aaGCTGCGGGTGATTGCAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCGGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTaaca                                                                                                                                             <  2:138657/251‑1 (MQ=255)                                                                                             gCTGCGGGTGATGGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGTCTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTaacaac                                                                                                                                           <  1:435622/251‑1 (MQ=255)                                                                                                 cGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACaaa                                                                                                                                        >  1:8089/1‑250 (MQ=255)                                                                                                     ggagtttAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGACTGCCGTCAGACAAAATCAGCTCGCAACCTGCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGCCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAaca                                                                                                                                   <  2:57062/245‑1 (MQ=255)                                                                                                         tGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGCGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGtt                                                                                                                               >  1:32187/1‑251 (MQ=255)                                                                                                              cagttgcttgccgccacccatgacgccATACCGCCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCATAGGCGCTCACCTCCGGATAAGCCGGCAGAGGGGAGGTCATGGATGTGTAGGGGTGCAGGATATGTCGTTGGTCAAAGGCAAGATAGCCCGTTGTCATAATCGACTTGTAAACCAAATGGAAAAGATTTAGGTGTACAAGTCTACACCGAATTAACAACAAAAAACACGGTTTGga                                                                                                                          <  2:362813/230‑1 (MQ=255)                                                                                                                      cgccgcCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGGCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGccc                                                                                                                   <  2:444595/250‑1 (MQ=255)                                                                                                                         cgccCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATg                                                                                                               <  1:469379/251‑1 (MQ=255)                                                                                                                                                             gCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTcac                                                                            <  1:327486/250‑1 (MQ=255)                                                                                                                                                              ccGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAg                                                                          <  1:350807/251‑1 (MQ=255)                                                                                                                                                                gTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCCTGGCTGTGTATGGGGGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGATGCCCCAAGGCCAACCGCACAAGATGGAAATTGTCGCAAGTAACAGa                                                                         >  2:437263/1‑250 (MQ=255)                                                                                                                                                                   aGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAatta                                                                     <  1:285421/251‑1 (MQ=255)                                                                                                                                                                      cAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCCCGCTGGACATTGTCGCAAGTCACAGAATTAttt                                                                  >  2:105046/1‑251 (MQ=255)                                                                                                                                                                                         tCGGCTCTCACCACCGGAGAAACCGGAAGAGGGGAGGGCATGGATGGGTAGGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTTGCATAATCGCCTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAATCCCCATGGCTCACCTCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTGGAAAAACCGTTGCTGGa                                                 <  2:309242/249‑1 (MQ=255)                                                                                                                                                                                                       cggttAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGTTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGGCCGTTGTCATAATCGACTGGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAAATAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAgcg                                 <  2:108324/247‑1 (MQ=255)                                                                                                                                                                                                       cGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAgcg                                 >  1:152228/1‑251 (MQ=255)                                                                                                                                                                                                          aTAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCgcag                              <  1:140421/251‑1 (MQ=255)                                                                                                                                                                                                                 ggCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGTATCTGCTGTTTGAAGCGCAGCAGGTGc                       <  1:437263/251‑1 (MQ=255)                                                                                                                                                                                                                  gCAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGc                       >  1:275599/1‑250 (MQ=255)                                                                                                                                                                                                                  gCAGAGGGGAGGGCATGGATGTGTATGGGTGCAGGAGATGGCGTTGGTAAAAGGCAAGAGCGGCCGGTGGCATAATCGACTTGTAAACCAAATTGAAACGATTTAGGTTTACAAGTCTACACCGCATTAACAACAAAAAACACGATTTGGAGAAGACCCATGGATCACCGCCCCAGCTGGACATTGCCGCCAGTCACAGAATTATTTGAAAAACAGCTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGc                       >  2:130337/1‑250 (MQ=255)                                                                                                                                                                                                                   cAGAGGGGAGGTCATGGATGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCAt                     >  1:240706/1‑251 (MQ=255)                                                                                                                                                                                                                       ggggtgggcATGGAGGTGTATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCAAAATCGACTTGTAAACCAAATTGAAGAGATTTAGGTTTACAAGTATACACCGAATTAACAACACAAAACACGATTTGGAGACGCACCATGGCTCACCGCCCACGCTGGTCATTATAGCAAGTAACAGAATTATTTGACAACCCGTAGCTGGATCTGTAGTTTGGAGCGCAGCAGTTGCACCGcc                 >  2:321003/9‑251 (MQ=255)                                                                                                                                                                                                                                aTGGATGTGTATGGGAGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCg        >  1:260837/1‑251 (MQ=255)                                                                                                                                                                                                                                      gtgtATGGGTGCAGGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTc  <  1:105046/251‑1 (MQ=255)                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                              TTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGGCAGAGGGGAGGTCATGGATGTGTATGGGTGCCAGATATGGCGTTGGTCAAAGGCAAGATCGTCCGTTGTCATAATCGACTTGTAAACCAAATTGAAAAGATTTAGGTTTACAAGTCTACACCGAATTAACAACAAAAAACACGTTTTGGAGAAGCCCCATGGCTCACCGCCCACGCTGGACATTGTCGCAAGTCACAGAATTATTTGAAAAACCGTTGCTGGATCTGCTGTTTGAAGCGCAGCAGGTGCATCGCCAGCATTTCGATCCTC  >  NC_000913/808976‑809455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑