Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3630C.89.3% 88.9 / 5.8 28intergenic (‑490/+919)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base . (21/4);  total (21/7)
Fisher's exact test for biased strand distribution p-value = 1.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
*NC_0009132,173,3640C.89.3% 88.9 / 5.8 28intergenic (‑491/+918)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base . (21/4);  total (21/7)
Fisher's exact test for biased strand distribution p-value = 1.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

AGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGG  >  NC_000913/2173128‑2173609
                                                                                                                                                                                                                                            ||                                                                                                                                                                                                                                                      
aGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGCGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACAAAACCAGCAATTAAAATGGAGAG‑AGGGTTAAAAATCAGGCTTGACGATACCACA‑‑GCCG‑TATGTCCcagc                                                                                                                                                                                                                                        >  2:90172/1‑250 (MQ=255)
           gggTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGGGATGCTCATAATGATGACACCCGAGCTTAAGGTGCGGAACAGATTTCCACGATGAACGGCGACCGCCATAGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGCCAACACCTGCTTAACCGGCAAACAAACAGCAATTAAAATGGTGAG‑TGAGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑AATGTCCAAGCAGCAACGCCga                                                                                                                                                                                                                             >  2:373367/1‑249 (MQ=255)
                         aTAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCAAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATAAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATAAAGGCCAATc                                                                                                                                                                                                                >  2:353654/1‑249 (MQ=255)
                               tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTc                                                                                                                                                                                                        >  1:54379/1‑251 (MQ=255)
                                gCGTCGCGATCCACAGGGTGATCCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGAGGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACACCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTcc                                                                                                                                                                                                       <  1:353654/251‑1 (MQ=255)
                                                             atgatgACAGCCGAGATTAAGGAGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGGTTGTGGCCAGACAGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGTTAAAAATCAGGCTTACCGCTACCACC‑‑GCCG‑GATGTCACAGCCGCAACCACGGATCAAGGCCAATCCGGAACGCCTAACCGCCGAACTTAGCCTGTAAACGAc                                                                                                                                                                           >  2:290529/1‑250 (MQ=255)
                                                                                              tttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGAGCGTTAAAccatc                                                                                                                                         >  1:81205/1‑251 (MQ=255)
                                                                                               ttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatcc                                                                                                                                        >  1:193322/1‑251 (MQ=255)
                                                                                                   aCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGTACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCACTACACCATCCatg                                                                                                                                     >  1:383460/1‑250 (MQ=255)
                                                                                                          cgggcacccGCCAGCGCCGCGAAAAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGGGAG‑GGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGAACAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGttt                                                                                                                             <  1:441768/244‑1 (MQ=255)
                                                                                                                            cctaaaaagCCGAGGGTGGCAAGCTCGCAAAACTGCAGCCCTTGTTTCCCCGGCACACAAACAGCAATTAAAA‑GGTGGGGTGGGATAAAAATCAGGCTTGCCGTTACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTag                                                                                                           <  1:302520/245‑1 (MQ=255)
                                                                                                                               aagaagCCGATGGTGGCAAGATCGCCAAACGGAAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGAAAAAAATCAGGCTGGCCGATAC‑‑CGCCGCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagcag                                                                                                        <  1:135793/251‑1 (MQ=255)
                                                                                                                                                      gCCAACCGGCAGCCCCTGATTACCAGGCACCCAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGAAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGACCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGGTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCg                                                                                 >  2:220305/1‑251 (MQ=255)
                                                                                                                                                           aCGGCAGCACCTGATTACCCGGCACAAAAACAGCAATTAAAATGGTGAG‑AGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGATTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTGTTACCGCCAGc                                                                            >  1:369908/1‑251 (MQ=255)
                                                                                                                                                             ggCAGCACTTGATTACCCGGCCCCCAAACAGAAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTg                                                                          <  1:8592/251‑1 (MQ=255)
                                                                                                                                                             agcagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGCG‑TGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGAGCGATAAACCATCCATGATGTGTTTAATCACCCATGGCATTAGCAGAATCACTGCCGCCGTTTATACCGCCAGCTg                                                                          >  2:251/2‑251 (MQ=255)
                                                                                                                                                                 cccgatctttccccGGCACAAAAACAGCAATAAAAAGGGGGAG‑GGGGATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAAt                                                                      <  1:99017/240‑1 (MQ=255)
                                                                                                                                                                      tGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACAcc                                                                 >  1:348173/1‑251 (MQ=255)
                                                                                                                                                                      tGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTCCCGGCGTTTTTACCGCCCGATGCAATACAcc                                                                 >  2:312791/1‑251 (MQ=255)
                                                                                                                                                                         ttACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATagttcgg                                                               >  2:99017/1‑244 (MQ=255)
                                                                                                                                                                                          aGCAATTAAAATGGTGAG‑AGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCAG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGTACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGAGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcgcg                                              >  1:282353/1‑250 (MQ=255)
                                                                                                                                                                                                         gAG‑TGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGCGGCATTAGCAGCATCCCTGCCGCCGTTTTTACCGCCAGCTGCCATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc                               >  2:36143/1‑250 (MQ=255)
                                                                                                                                                                                                               ggctAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGAATCCAGGCCAATCAGAAACTCATAACCGCCGAACTTCACATGTAAACGACTACAAGCCTGCTTAGCGATAGGCGTTAAACCATACATGATGGAGTTCATCACCCGTGGACTTAGCCGCCTCACGTCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGAAAACGTAACCCGCGAGGGTGCCGCTACTCAAcccc                         >  2:136142/4‑251 (MQ=255)
                                                                                                                                                                                                                  tAAAAATCAGGCTTGCCGATACCACC‑‑GCCG‑AATGTCCAAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTACGCATGTAAACGACTACGAGCCTGCTTAGCGATGGGAGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGAGAGGATGCCGATAATCAGCACCATc                      >  1:158892/1‑251 (MQ=255)
                                                                                                                                                                                                                         cAGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCAGGGATGCCGATAATCAGCCCCATCACAAAAc               >  2:126948/1‑251 (MQ=255)
                                                                                                                                                                                                                          aGGCTTGCCGATACCACC‑‑GCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAcc              >  2:391057/1‑251 (MQ=255)
                                                                                                                                                                                                                           cgggctgccggacccccccGCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGCGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAc               <  1:36143/236‑1 (MQ=255)
                                                                                                                                                                                                                            gCTTGCCGATACCACC‑‑ACCG‑TAGGTCCCCGCAGCCCCGCCGGATCACGGCCAATCATGAACTCCTGACCGCCGCACTTCGCCTGTAAACGACTACGAGACTGCTTCGCGACGGGCGTTAAACCCTCCATGATGGGTTTAATCACACGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATCAACCTTTGACATCGGAACCCGCTAGGATGCCGATAAGCAGCCCCACCACAAAAccc             >  2:76714/1‑250 (MQ=255)
                                                                                                                                                                                                                                      gccaccCCCCCGGTATGTCCCGCCACCAACGCCGGATCAAGGCCAACCAGAACCCCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCAGCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGAc     <  2:54379/250‑1 (MQ=255)
                                                                                                                                                                                                                                        accaccgccg‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGAGACAgg  >  2:451213/5‑251 (MQ=255)
                                                                                                                                                                                                                                            ||                                                                                                                                                                                                                                                      
AGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAG‑TGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCG‑TATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGG  >  NC_000913/2173128‑2173609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑