Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913480,2950GA100.0% 84.3 / NA 28P5F (CCT→TTT) hhahemolysin expression‑modulating protein Hha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/15);  total (13/15)
*NC_000913480,2960GA100.0% 79.0 / NA 28P5F (CCT→TTT) hhahemolysin expression‑modulating protein Hha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/15);  total (13/15)

AGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGT‑CAATTCGGCGAGGCGGTGATCTGCGGCTG‑AGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAG  >  NC_000913/480068‑480538
                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                                  
aGCTGCGAGAGCGAATTATTGATTAGCGAATAAATTTCCATGCTGAGGAAGGGGTCGTGTCGTACAGTTTATGCATGGT‑CAATGCGGCGAGGCGGTGATCTGCGGCTG‑AGTAAAATACCGCCAGTTCATTATCTGATAAGTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGGCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTGCGTTAAAAATTTTTCGGACATACTTCTACCt                                                                                                                                                                                                                              <  1:343880/251‑1 (MQ=255)
     gaaaagcaaatgttttattAGCGAAAAAATTTCCATATTGAGGAAGGGACTTTGTCGTACAGTTTATACTGGGT‑CATTCCGGCGGGTGGGTAATCTGCGG‑TGTAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGt                                                                                                                                                                                                                         <  2:241529/238‑1 (MQ=255)
             aaTTATTTATTAGCTAAAAAATTTCCATACGGAGGAAGGGATCTTGGTGTACAGTTTATTCA‑GGTACAATTCGGCGAGGCGGAGATCTGCGGCTA‑AGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGtaataa                                                                                                                                                                                                                 <  2:266048/251‑1 (MQ=255)
                tatttattAGCGAATAAATTTCCATACTGAGGAAGGGAACTTGTCGTACAGTTTATTCATGGT‑CAATTCGGCGAGGCGGTGATCTGCGGCTG‑AGTAAAATACCGCCAGTTCATTATATGATAATTCGTATTTATTTTTCTCGATAACACGCTCCGGCGTGTTAATTTTCTTGCAACGTCGTAAACGCATTAAATAATCGGTTTTCTGTAAAAATTATTTGGACATACTTCTAACTATTGTTGtaaatattt                                                                                                                                                                                                              >  2:293496/1‑245 (MQ=255)
                       taGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGT‑CAATTCGGCGAGGCGGTGATCTGCGGCTG‑AGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACaa                                                                                                                                                                                                        <  1:267390/250‑1 (MQ=255)
                                                      tCTTGTCGTACAGTTTATTCATGGT‑CAATTCGGCGAGGCGGTGATCTGCGGCTG‑AGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCAtt                                                                                                                                                                        >  1:232/1‑251 (MQ=255)
                                                                                           ggCGGGGATCTGCGGCTG‑AGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTATCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTccc                                                                                                                                    >  2:350895/1‑250 (MQ=255)
                                                                                             cGGTGATCTGCGGCTG‑AGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGATTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTCCCTGACTTCCGcc                                                                                                                                  >  1:229860/1‑251 (MQ=255)
                                                                                                            g‑AGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTAc                                                                                                                                                                                                                                <  1:233019/142‑1 (MQ=255)
                                                                                                            g‑AGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTAc                                                                                                                                                                                                                                >  2:233019/1‑142 (MQ=255)
                                                                                                                tAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGCCTTCCGCCATTTCTGAAGATACTGc                                                                                                                 >  2:294002/1‑250 (MQ=255)
                                                                                                                  aaaTACCTCCAGTTCATTATCTGATAATTCGTATTTATTTTTCCCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCTGTTTTCGGTAAAAATTTTTCGGACATACTTCTACCTATTGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCata                                                                                                              <  2:178980/251‑1 (MQ=255)
                                                                                                                     tgccGCCAGTTCATTATCTTATAATTCGTATTTATTTTTCTCGATAACACGCGCCAGCGTGTCAATTTTCTTGCAACTACGTAAACGCATTAAACAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTTTGGTTGTAATAATTCTATCAAGATAAACTTTCAGGATTCTCTTTCGTGGCTTTTACAAAACAACGGAATAGCTGATTCCCTGACTTTCGCTATTTCTGACGATCCTGCATAtt                                                                                                            <  2:429928/248‑1 (MQ=255)
                                                                                                                      aCCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTa                                                                                                           >  1:29048/1‑250 (MQ=255)
                                                                                                                        cGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAAt                                                                                                         >  1:67789/1‑250 (MQ=255)
                                                                                                                            aGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCATGCATATTAATACCAt                                                                                                    >  2:258570/1‑251 (MQ=255)
                                                                                                                              ttCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCCTTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCCTATTAATACCATa                                                                                                   >  2:419623/1‑250 (MQ=255)
                                                                                                                                                         tttCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATc                                                                        <  1:378621/250‑1 (MQ=255)
                                                                                                                                                                  aaCACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATtcg                                                              <  2:450282/251‑1 (MQ=255)
                                                                                                                                                                               gtgtCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCTTAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTAGATCTGCTCa                                                  >  2:229678/1‑250 (MQ=255)
                                                                                                                                                                                            ggCAACGACGTAAACGCATTAAAGAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGTTTGTAATAATTCTATCAAGATAAACTCGCAGGATTCGCTTTCGTCGCATTGACAAAACAACGGAATAGTCGAATACCTGACTTCCGCCATTTCTTAAGATCCTGCATATTAATACCATAACTACGGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTg                                      <  1:31348/249‑1 (MQ=255)
                                                                                                                                                                                                 cgacgTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCAt                               <  1:350895/251‑1 (MQ=255)
                                                                                                                                                                                                     gTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATtata                           <  1:46494/251‑1 (MQ=255)
                                                                                                                                                                                                     gTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCATGCATATTAATAACATAACTACTGAACAACATAAAGGTGTCATCTAGATATTCGTCGATCTGCTAAATGAGATTATTGTCTTCATtat                            >  2:200064/1‑250 (MQ=255)
                                                                                                                                                                                                      tAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATAc                          >  1:57780/1‑251 (MQ=255)
                                                                                                                                                                                                       aaaCTCATTAAATAATCGGTTTTCGTTAAAAATTTTACGGACAAACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACt                         <  2:109555/251‑1 (MQ=255)
                                                                                                                                                                                                           gCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCTTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAAt                     <  2:67789/251‑1 (MQ=255)
                                                                                                                                                                                                                              cGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAg  <  1:419623/251‑1 (MQ=255)
                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                                  
AGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGT‑CAATTCGGCGAGGCGGTGATCTGCGGCTG‑AGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGTGCGAAG  >  NC_000913/480068‑480538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑