| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,084,903 | T→G | N307T (AAC→ACC) | fdoG ← | formate dehydrogenase O subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,084,903 | 0 | T | G | 91.7% | 182.8 / ‑2.2 | 60 | N307T (AAC→ACC) | fdoG | formate dehydrogenase O subunit alpha |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base G (27/28); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GGAACGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACG > NC_000913/4084661‑4085145 | ggAACGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCCCGATCAGGCTGGCGGTgggct > 1:463621/1‑246 (MQ=255) gAACGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAgg < 1:335271/251‑1 (MQ=255) aCGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTGCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCt < 1:15775/251‑1 (MQ=255) cgcgTCTTTTGGCGTTCCACAGATGTTTTCATCCACATCTGGCGTGTAGCGGTAAACGTGCTGGTTCAGTAATTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCTTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTACCGGACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACTGGCCATCTT‑CAAAGCCGTAATCCCCACGTACGATCAGGCTGGCTGTGGTATAGGCtt < 1:103512/251‑1 (MQ=255) gTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATTGGCTTTGGTGTATTcgcg > 2:42102/1‑251 (MQ=255) caGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGGATAGGCTTAGGTGTAtttccgtgtga > 2:177397/1‑241 (MQ=255) gATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAAtt < 1:240436/251‑1 (MQ=255) ttCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTctt > 1:354833/1‑249 (MQ=255) tCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGGGGTATAGGCTTCGGGGTATTCGCGGTTGAATTTTTCATTGTTc‑agc > 1:402109/1‑249 (MQ=255) cTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAgg > 1:145176/1‑251 (MQ=255) aGCGGGAAACGTGCCGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAAt < 1:64956/251‑1 (MQ=255) gCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATa < 2:132796/251‑1 (MQ=255) aaCGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGAGTTTTTCCGCGTCGTAGCCGGTGACCAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGGGGAGGTAAGGGCTTCGGGGTATTCGCAGTTGAATTTTTCATTGGTC‑AGAAgggccccaaaatcgcctg > 2:259985/1‑235 (MQ=255) tGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGGATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTAAAGCAATACCCCTGAc > 1:446348/1‑249 (MQ=255) tGTTTCAGCAAGTTCCACACGCCGCGCGGATGTTGCAGCGTGGTTTCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAGGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTTGTAGCCGGTGAACAGGCAATCTT‑CACAGCCGTAATCCTCACGCCCGATAAGGCTGGCGGTGGTTAAGGCTTCGGTGTTTTCGCGGTGGCATTTTTCCTTGTTC‑AGCAGGTAccgccttaagcccgacagg > 2:18084/1‑231 (MQ=255) cAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCaa < 1:13605/251‑1 (MQ=255) gCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATg < 1:410542/251‑1 (MQ=255) aGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGCTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGGATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGGAAGCAATGTc > 1:301464/1‑250 (MQ=255) tCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTAt < 1:378047/185‑1 (MQ=255) ccACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGATTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATAAGCCTGACAGGAAAGAAATGTCAgat > 1:84374/1‑248 (MQ=255) cacGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGc < 1:416810/224‑1 (MQ=255) cacGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGGACAGCAATAccc > 2:416810/1‑222 (MQ=255) ggTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACCGGAAAGCAATGTCAGTACCGGCACGAATAGGGGCAGAGGAGTc > 2:298834/1‑249 (MQ=255) gTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGc > 1:388363/1‑251 (MQ=255) aTCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTACTAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCACGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGGATAGGCTTCGGGGACATCGCGGTTGAATTTTTCATTGTTC‑AGCCGGTACAGCCATACGCCTGACCGGAGAGCAATGTCAGTACCGGGACGGATAGGGGCATAGTAGTCAGCt > 1:427609/1‑250 (MQ=255) gTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAccgcc < 1:102889/251‑1 (MQ=255) ggCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTGGCGTTTTGCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAccgccgcc < 1:42102/250‑1 (MQ=255) agacGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTAcgc < 1:164774/248‑1 (MQ=255) gTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCGCGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTAAAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAAGCAGCTACCGCCGCCGTACGCGTa > 1:73056/1‑250 (MQ=255) gaccAGTTCAAAAGGCAAGGATGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGGGAACAGGCCATCTT‑AAAAGGCGTAATCCTCACGCACGATCAGGCTGGGGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAAGGTCAGTACCGGAACGAAGAGGGGAATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAg < 2:189465/249‑1 (MQ=255) ccAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGga < 1:318006/251‑1 (MQ=255) cAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGgat < 1:469329/251‑1 (MQ=255) gtcaaTAATTCCAGGAGGATTGATCGTATTGGTGTTTTTCCGCGTCGTAGCCGGGGGACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTACGGTGTAGTCGCGGTTGAATTTTTCATTTTTC‑AGCAGGGACAGCTATACGCCTGACAGGAAAGCAATGTCAGGACCGGAACGAATAGGGGAAAAGTAGTCAGCTCCCGCCGCCGTACGCGTAAAGCGAGgat < 2:231013/245‑1 (MQ=255) gTCCAGGAGGATTGATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAAt < 1:160114/251‑1 (MQ=255) aggaggATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGAGCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGAAAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCAACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGc < 2:367703/251‑1 (MQ=255) tATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGGTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCATGACAGGAAAACACTGTCAGTGCCGGAAAGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCg > 1:213714/1‑251 (MQ=255) cgtcctggCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGtt < 1:228288/243‑1 (MQ=255) cGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGTCTCGGGGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGACTGAAAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGGAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTtg > 1:5896/1‑251 (MQ=255) aCTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCTTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCATTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGa < 1:71632/251‑1 (MQ=255) aCTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAACTACCGCCGCCGTACGCGTAAAGCGAGGATCGATAACAAACAGCTTCGCGCCGTtgtg > 2:142031/1‑250 (MQ=255) tGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGGGGTATAGGCTTCGGTGTATTCGCGGTTGCATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCGGTCCCGGAACGAATAGGGGCATAGTAGTCCGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGCTCACAATCAGCTTCGCGCCGTTGTGAAtt > 1:283594/1‑251 (MQ=255) gCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGGGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGGACAGCAATACGCCTGACAGGAAAGCAATTTCAATACCGGAACGAATAGGGGCATAGTAGGCAGCTACCGCCGCCGTAAGCGTAAAACGGGGATCGATCCAAATCAGCTTCGCGCCGTTGTGAAttt > 2:322530/1‑251 (MQ=255) ttttCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCTCCGTTGTGAATTTTgg < 1:419946/250‑1 (MQ=255) gtttCCGCGTCGTAGCCTTTGAACAGGCC‑TCTTTCAAAGCCGTAATCCTCACGCTCGATCAGTCTTGCGTTGGTATAGGCTTCGGTGTATTCGCGTTTGAATTTTTCATTGTTT‑AGCAGCTACATCAATACGCCTGACAGAAAAGCAATTTCAGTACCGGAACGAATAGGGGCATATTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGATGATCGATCACAATCAGCTTCGCGCCTTTGTGAATTTTgg < 1:419954/249‑1 (MQ=255) cgtcgtAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCAGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTA‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGGCAGTACCGGAACGGATAGGGGCATAGGAGTCAGCTACCGCCGCCGTAAGCGTAAAGCGAGGAGCGATCACAATCAGCTTCGCGACGTTGTGAATTTTGGCTTCCATc > 1:435354/1‑251 (MQ=255) tcgtAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGACTAGGGGCATAGTAGTCAGCTACCGCCGCCGGACGCGGAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGc > 2:274436/1‑251 (MQ=255) tAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCCAAGTCAGTACCGGAACGGATAAGGGCATAGTAGTCAGCTAACGCCGCCGTACGCGTAAAGCGAGGGTCGGTCACAAGCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATAGcccc > 2:295999/1‑250 (MQ=255) tAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGAATAGGCTTCGGTCTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAACTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGcccc > 1:66183/1‑250 (MQ=255) gaGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCa < 1:309768/250‑1 (MQ=255) agccgtggAACAGGCCATTGT‑CAAAGCCGTAATCCTCACGCACGATCTGGCTGGCGGTGGTATAGGCTTCGGTTTATTCGCGGTTGAATTTTTCAT‑GTTCCAGCAGGAACAGCAATACGCCTGACAGGAAATCAATGTCAGTACCGGATCGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCa < 2:127483/243‑1 (MQ=255) ggTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCCTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCCGTACCGGAACGAATAGGGGCATAGTAGGTAACTACCGCCGCCGCAAGCGGAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGGTGTGAATTTTGGCTTTCATCGCCCAGCgg > 2:172652/1‑250 (MQ=255) ggAACATGCCATCTT‑CAAAGCCGTAATCCTCCCGCTGGATCAGGCTGGCGGTGGTAGTGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATCCGCCTGACAGGAAAGCAATGTAAGTACCGGAACTAATAGGGGCATATTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAAc < 2:378849/250‑1 (MQ=255) gAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAAc < 1:363485/250‑1 (MQ=255) gTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCTCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAgcgg > 1:316395/1‑251 (MQ=255) tAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAAAGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGACCAGCGGAACCCGACCAGGTGAGCTTCAgcggg > 1:225012/1‑250 (MQ=255) aacccTCCCGCACGATCTGGCGGGCGGTGGTATAGGCTGCGGTGTATTCGCGGTTGAATTTTTCATTTTTC‑AGCAGGTACAGAAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACAGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAgcggc < 2:354833/247‑1 (MQ=255) aaTCCTCACGCACGATCAGGCTGGCGGTGGGATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATTTCAGTACCGGAAAGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGAACGCGTAAAGCGAGGAGAGATACCAAACAGATTCGCGCCGTTGGGAATTTTGACTTCCATCGCCCAGCGGAACCCGCCCGGGGGGGCTTCaacagtg > 2:349287/1‑245 (MQ=255) aTCCTCACTCACTTTCGGGCTGGCGTGTGTATAGGTTCCTGTGTATTCTCGGTTGAATTTTTCATTGTCC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTCCGGGAACGAATAGGGGCATAGTAGTCATCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGt < 2:388363/251‑1 (MQ=255) gATCAGTCTGGCGGTTGAATAGGCTTCGGTGAATTCGCGGTTGAATTTGTCATTATTC‑AGCAGGTACAGCAATCCGCCTGACAGGAAAGCAATGTCAGTTCCGGAACGAATAGGGGCATAGTAGTCAGCTCCCTCCTCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATca < 1:133123/251‑1 (MQ=255) ggCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGAATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCacg < 1:295999/251‑1 (MQ=255) | GGAACGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTT‑CAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTC‑AGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACG > NC_000913/4084661‑4085145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |