Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,641	T→A	R380R (CGA→CGT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,641	0	T	A	94.4%	40.9 / NA	18	R380R (CGA→CGT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (9/8);  minor base C (1/0);  total (10/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACC‑GAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCT  >  NC_000913/64402‑64878                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                gagaACCAGGCATCGAGAAAACCTTCGGTACTGGGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAAGCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATa                                                                                                                                                                                                                                      <  2:218783/251‑1 (MQ=255)     aCCAGGCATCGAGAAAACCTTGGGTACTGGGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGGGCGGATTATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAgaga                                                                                                                                                                                                                                  <  2:399455/251‑1 (MQ=255)                  aaaaCCTTCGGTACTGTGATCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACgccg                                                                                                                                                                                                                     >  1:198567/1‑251 (MQ=255)                                          aacAGGCTGCGCCATGCCGTCCACCAGCCCGGCGGGATCAATCAGAAAGGGGCGGATGAACGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTcta                                                                                                                                                                                            <  2:171170/249‑3 (MQ=255)                                               gCTGCGCAAGGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGAGGAGCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAAGCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTTGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGTTCTACCGGc                                                                                                                                                                                       <  2:429252/251‑1 (MQ=255)                                                    gcCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCCGCACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGACAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGCTGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGcc                                                                                                                                                                                  >  1:59492/1‑251 (MQ=255)                                                      cATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGCTGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGACGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTTGGCGCGACATAACCAGCGCGGTGCATCAGAGGAAAATAGAGATGACCCAACGCC‑CAACCCGATCCCCAGTGTCGGGCTACCGGCAGGCCgg                                                                                                                                                                                >  2:195725/1‑250 (MQ=255)                                                              ccaccaGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCGTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtc                                                                                                                                                                        <  2:167099/251‑2 (MQ=255)                                                                                     aGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGAGGTGAATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGGGGCGGGCTACCGGCAGGCCGGTCACCGTCGGCCGTTCGAGGAAAAAAGGCAt                                                                                                                                                 >  2:41186/1‑251 (MQ=255)                                                                                                                     tttaaagtccggacccagtaccgAATCATAAAGCCCGGGCCGTGAATCCATCACGTGGCCGCCAGGGCTGGCGTGGGGCGGCAATTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGGTCGAGTAAAAATGGCATGATTTCAGTGTTATGGAAAATCTGCGTCAcc                                                                                                                  <  2:220103/228‑1 (MQ=255)                                                                                                                                   ccagtaccgAATCATAAAGCCCTGGCCGTGAATCCATCAAGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCCGCGCGGTGCATACGAGGAAAATAGCGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCAACCGGCAGGCCGTTCACCGTCGATCGTTAGAGTAAAAATGGCATGATTTCAGTTTTATGGGAAATCTGAGTCACCAGCT‑CGCAATCTTTc                                                                                                  >  1:324921/9‑251 (MQ=255)                                                                                                                                                  aaaGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGGGTAAAAATAGCCTGATTTCCGTTTTATGGAAAATCTTCGTCACCAGCT‑CGCAATCTTTTCGGTTTTAAGTCGcc                                                                                   >  2:45512/1‑251 (MQ=255)                                                                                                                                                    aGCCCGGGCCGTGAATCCATCCCGTAGCCGCCAGGGCTGTCGTGTGGCGGCACTTCGCCGAGAGCAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAg                                                                                 <  2:174818/251‑1 (MQ=255)                                                                                                                                                             cGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGttt                                                                        <  2:451883/251‑1 (MQ=255)                                                                                                                                                               tGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCAACCGGCAGGCCGGTCACCGTCGGTCCTTCGGGTGAAAATGGCAGTATTTCAGTTTTATGGAAAATTTGCGTCACCATCC‑CGCTCACTTTCAGGCTATAAGtaccccacgccggtttat                                                                      >  2:371396/1‑234 (MQ=255)                                                                                                                                                                             aGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGGGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCCATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAAcgg                                                        >  1:242779/1‑251 (MQ=255)                                                                                                                                                                               ccgccAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGGCTACCGGCAGGGCGTTCACCGTCGCACGTTCGAGTAAAAATTGCATGATTTTAGTTTTATGGGAAATATTCGTCCACAGCTACG‑AAACTTTCAGGTTATCAGTCGCCCGCGCAGGTTTTTATTTGGCGAAAcggcg                                                      >  2:248861/1‑251 (MQ=255)                                                                                                                                                                                                                                   cAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGAATGATTTCAGTTTTATGGCAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCACCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTAAATTTTGTCCAATCGGACCCCCGGGTTATCGAGAGATTTTTCTTTGccc  >  1:421477/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                GAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACC‑GAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCT  >  NC_000913/64402‑64878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑