Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 84,251 G→T intergenic (‑543/‑117) leuL ← / → leuO leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091384,2510GT92.9% 63.6 / ‑0.1 28intergenic (‑543/‑117)leuL/leuOleu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (16/10);  total (17/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

ATTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCG  >  NC_000913/84005‑84494
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
aTTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAAtt                                                                                                                                                                                                                                                 <  1:70306/251‑1 (MQ=255)
aTTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAAtt                                                                                                                                                                                                                                                 >  1:348733/1‑251 (MQ=255)
       ttGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTc                                                                                                                                                                                                                                           <  1:383162/250‑1 (MQ=255)
                 tttGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCtttt                                                                                                                                                                                                                                >  1:17692/1‑251 (MQ=255)
                    ggTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCt                                                                                                                                                                                                                             <  2:443637/251‑1 (MQ=255)
                                  aTCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCAt                                                                                                                                                                                                               <  1:398714/251‑1 (MQ=255)
                                             ttAAGAATTAATGCATTAAATATATAAATTAATTCTTAAATAAGCACATTTAATCCATTTTGTAGTTGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCAACGGCAATGGATTATGTTTTTATCAGAACCCGTATATTTATGTTTTCCTAATTTTACTCATTTTCCTTTTGCTTATTTTATATGCATGTTAAATCatt                                                                                                                                                                                                    >  2:325724/1‑250 (MQ=255)
                                             ttAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCAAATTTAATCCATTTTGTAGATGATTGAGTATTCGAGGTAGTATTGATTAGATTGTTTTCGCAATAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCAACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGTTAAATCat                                                                                                                                                                                                     >  2:38513/1‑250 (MQ=255)
                                                                                  aaaTAAGCACATTTAAGTCATTGTGTAGATGATTGAGTATTCGCGGTAGTTATGATTATATTGTTTTCGCAACACAAACATTATGTATTATTATGCTTTGGTAAATGACTCATTCCACGGTAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACGCATGTTCCTTTGTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTACTCTGCATTCCAATaagtgat                                                                                                                                                               <  2:435853/251‑5 (MQ=255)
                                                                                   aaTAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATaagggaa                                                                                                                                                               >  1:275104/1‑250 (MQ=255)
                                                                                   aaTAAGCAAATTTACTCAATTTTGTAGGTGATTGAGTATTCGCGGAAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTCAATGGCTCATTCCACGACAATGACTTCTGTTTTTATCAGAACCCGGAGCTTTATATTTTCCTAATTTTACTCATTTTACGTTTTCATATTTTAGATGCATAATAAATCTTATACTCGAGGATTATTTCTATGCATTCCACTaagggaa                                                                                                                                                               >  2:136496/1‑250 (MQ=255)
                                                                                                       tttGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGTATTATTTCTCTGCATTCCATTAAGGGAAAGGGTGTTAAGTGTGACAGTg                                                                                                                                          >  2:18207/1‑251 (MQ=255)
                                                                                                           tAGATGATTGAGTATTAGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGAAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGt                                                                                                                                      >  2:182726/1‑251 (MQ=255)
                                                                                                                                                                       gATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTT‑ATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATTAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCt                                                                         >  1:89813/1‑251 (MQ=255)
                                                                                                                                                                           attaaGCTGTTGTAAATGACTCATTCCACGGCAATTGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAACCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTAc                                                                       <  2:172288/250‑1 (MQ=255)
                                                                                                                                                                             tatGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAAACCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGc                                                                     >  1:240277/1‑250 (MQ=255)
                                                                                                                                                                              atGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATaagggaa                                                                                                                                                               <  1:329010/159‑1 (MQ=255)
                                                                                                                                                                              atGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATaagggaa                                                                                                                                                               >  2:329010/1‑159 (MQ=255)
                                                                                                                                                                                gCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATgg                                                                 >  1:115746/1‑251 (MQ=255)
                                                                                                                                                                                              cTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACtt                                                    >  1:266369/1‑250 (MQ=255)
                                                                                                                                                                                                   tCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGAcgg                                                                                               >  2:333649/1‑202 (MQ=255)
                                                                                                                                                                                                   tCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGAcgg                                                                                               <  1:333649/202‑1 (MQ=255)
                                                                                                                                                                                                                  ttggTTTTTACCAGAACCCGGTTCTTTATTTTTTCCTAATTTTCCTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCGCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGTTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATgtcg                                <  2:249260/247‑4 (MQ=255)
                                                                                                                                                                                                                     gTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCATAGGTACAAACAGATCATCCAGAGACGGCGTAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGAt                            <  2:145684/251‑1 (MQ=255)
                                                                                                                                                                                                                                       aTCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATAATATTCTCGAGGTTTATTTCTCTGCATTCCAATATGGGAAAGGAAGTTAAGTGTGACAGTGGAGTTAAGTATGCAAGAGGTACAAACAGATCATCCAGAGCCGGCGGAGTTAAGCAAGCCACAACTACGCATGGTCAACCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGGGCAAAACATTat          >  2:25029/1‑250 (MQ=255)
                                                                                                                                                                                                                                              tGTTTTCCTAATTTTACTCATTTTCCTTTTTTTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGGGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGt                                           <  2:354066/211‑1 (MQ=255)
                                                                                                                                                                                                                                              tGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGt                                           >  1:354066/1‑211 (MQ=255)
                                                                                                                                                                                                                                               gTTTTCCTAATTTTACTCCTTTTCCTTTTTCTTATTTTATATGCTTGATAAATCATCTTCTCGAGGATTCTTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGCGACGGCGGCGTTAAGCAAGCCACCGCTACGCATGGTCGATCTCAACTTCTTAACCGTTTTCGATGCCGTGATGCAGGGGCAAAACATTACTCGTGCCg  >  2:438287/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
ATTTTTTTTGATATTGATTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCG  >  NC_000913/84005‑84494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑