Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,629,921 C→T E228K (GAG→AAG)  yhiI ← putative membrane fusion protein YhiI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,629,9210CT95.1% 179.5 / ‑1.2 61E228K (GAG→AAG) yhiIputative membrane fusion protein YhiI
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (26/32);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01

CATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGC  >  NC_000913/3629679‑3630153
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
cATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCACAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGGCATATAGACGTCGCTGAGATCGACCATATTCAACACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAAccc                                                                                                                                                                                                                                     >  1:225572/1‑251 (MQ=255)
     gTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGGAGAAGGTCATATAGACGTCGCTGAGATCGACCATTTTCAGCACCCGACCGACTGCCGCAAGCCCTTCCCCTGGCTTGGCACCCCGTTAc                                                                                                                                                                                                                                >  2:213661/1‑251 (MQ=255)
      tttCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAAccagctcgg                                                                                                                                                                                                                               >  2:126249/1‑244 (MQ=255)
      tttCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAAcc                                                                                                                                                                                                                                      <  1:126249/244‑1 (MQ=255)
        tCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGCTGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCCCTTCGACTGGCTTGGCAACCCGATACTGc                                                                                                                                                                                                                             >  2:413092/1‑251 (MQ=255)
              gTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGcgt                                                                                                                                                                                                                       >  1:209880/1‑251 (MQ=255)
                 caccGCTGGTTCCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATCCGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGcgtcc                                                                                                                                                                                                                     <  1:244796/247‑1 (MQ=255)
                       tGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTAGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCAGTCAcgc                                                                                                                                                                                                              >  1:359232/1‑251 (MQ=255)
                        ggTTTCGAGGGTTTTTGGCGGAAATTGGGCGCCATGGGTGACAAAACTGATGGTGGCGGAAATCGGCAGATCTGGCGCGGC‑TCGGGGGTCCAGCCGGGCTGCACCGCCCATTTCCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCAcgcg                                                                                                                                                                                                             <  2:192933/251‑1 (MQ=255)
                          tttCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGAGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGACAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCggg                                                                                                                                                                                                           >  1:417304/1‑251 (MQ=255)
                           ttCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGTAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATCATGCACGCCTCCTTCACGCgggg                                                                                                                                                                                                          >  2:146110/1‑251 (MQ=255)
                            tCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGc                                                                                                                                                                                                         >  2:411969/1‑251 (MQ=255)
                            tCGACGGTTTTTGGCGTGAACGGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCgggg                                                                                                                                                                                                          <  1:307929/250‑1 (MQ=255)
                               aCGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCttt                                                                                                                                                                                                      >  2:30376/1‑251 (MQ=255)
                                        ggCGTGACCGGGTCGACACTGGCGACAAAACTGAGGGTGGCAGGATTACGCAGATCTTGCGCGGCAGCGAGGATC‑AGCCGGGCTTCCCCGCCTATTTTCGGCGTGCCCGCCTGTTCGGTTGGCAGGAATAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCt                                                                                                                                                                                             <  1:343344/251‑1 (MQ=255)
                                                   ggcgacccgtgcgccAAACCTGTTGGTTGCCGGAATACGCAACTCGGGCCGGGCATCGAGGATC‑AGCCGGGCTTCCCCGCCCAGTTCCAGCGTCCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGACCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGatat                                                                                                                                                                                  <  1:15260/237‑1 (MQ=255)
                                                    gggacgcgggggacaaaacggtgggTTGCAGGAATACGGAGATCGGGCGGGGCATCGAGTATC‑GGCCGGGCTTCACCCCCCATGTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGGCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGGCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATc                                                                                                                                                                                 <  2:432769/228‑1 (MQ=255)
                                                       acacTGGGGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGACC‑AGCCGGGCTTCACCGCCCATTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGc                                                                                                                                                                              <  1:30952/251‑1 (MQ=255)
                                                        cacTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCg                                                                                                                                                                             >  1:130595/1‑251 (MQ=255)
                                                          cTGGCGACAAAACTGATGGTTGCAGGAATCCGCAGATCTGGCGCGGCATCGAGAATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCCTCGATATCTGCGGc                                                                                                                                                                           >  1:328565/1‑251 (MQ=255)
                                                                            gTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGACCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCAGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGc                                                                                                                                                         >  1:61968/1‑251 (MQ=255)
                                                                               gCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTCCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTtg                                                                                                                                                      <  1:259201/251‑1 (MQ=255)
                                                                                       aCGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCtt                                                                                                                                             >  1:248880/1‑251 (MQ=255)
                                                                                       aCGCAGATCTGGCGAGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGACGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGCTACTGCCCGCGTCCGACACGCGGGGCTTTCAGTTCGCTGTCATCGATATCGTCGGAACTGCGCCGTGCCGTGGCTTG‑GGCCGCtt                                                                                                                                             >  2:108069/1‑251 (MQ=255)
                                                                                          cAGATCGGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGa                                                                                                                                          <  1:411969/251‑1 (MQ=255)
                                                                                           aGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGCAAGTCATATAGACGTCGCTGCGATCGACCATATTCAGCACCCGACCACCTGCCGCCCGCACTTCGCCTGGCTTGGCAACCCGATCCTGCACGCGTCCGTCCAGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGCGTCTTG‑TGGCGCCTCggc                                                                                                                                         >  2:100515/1‑249 (MQ=255)
                                                                                           aGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCCTCGCTATCTGCGGCAATGAGCCGTTCAGTGGCTTGGTGCCgttccac                                                                                                                                          >  2:192937/1‑245 (MQ=255)
                                                                                                gggCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCggg                                                                                                                                    <  1:30376/250‑1 (MQ=255)
                                                                                                 ggCGCGGCATCGAGGATC‑AGCCGGGCTCCCCCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCgg                                                                                                                                     <  1:221808/249‑1 (MQ=255)
                                                                                                  gcgcGGCACCGAGGACC‑AGCCGGGCTTCACCGCCCATTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGtt                                                                                                                                  <  1:151172/251‑1 (MQ=255)
                                                                                                   ggcgGCTTCGAGGATC‑AGCCGGGCTTCCCCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGttt                                                                                                                                 <  1:385223/250‑1 (MQ=255)
                                                                                                   cgcgGCATCGAGGATC‑AGCCGGGCTTCCCCGCCCAGTTTCAGCGGTCCCGCCTGTTCGTTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGTCCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGttt                                                                                                                                 <  1:72426/251‑1 (MQ=255)
                                                                                                           cGAGGATC‑AGCCGGGCTTACCCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGa                                                                                                                        <  1:463404/251‑1 (MQ=255)
                                                                                                            gAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGTCGCTTCTCCGCGGGGTTGCGCC‑Tgg                                                                                                                        >  1:355630/1‑249 (MQ=255)
                                                                                                                  c‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGatat                                                                                                                 >  1:203557/1‑251 (MQ=255)
                                                                                                                    aGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTAACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCGTCTACGCGGGTTTGCGGC‑TGGATGAAAtt                                                                                                                >  1:34464/1‑251 (MQ=255)
                                                                                                                      ccGGGCTTCCCCCCCCATTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCTCC‑TGAATGATATTgg                                                                                                              <  1:367082/251‑1 (MQ=255)
                                                                                                                                ccGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGAGCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTTCGCC‑TGAATGATATTTGTT‑CGTGCCGc                                                                                                   >  1:245079/1‑251 (MQ=255)
                                                                                                                                  ccgatgttccggcgtgccgtcttGTTCGGTTGGAAGGAAGAAAGTCATAATGACGCCGCTGAGATCGACCAAATTCAGCCCCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGTTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGGG‑CGTGCCGCtt                                                                                                 <  2:425084/229‑1 (MQ=255)
                                                                                                                                      attctcgcctgccccGCTTGGTCGGTTGGCAGGAAGAAAGTAATATAGCCGTCGCTGAGATCGACCATATTCAGCCCCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCtat                                                                                             <  2:151055/239‑1 (MQ=255)
                                                                                                                                         ttCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAg                                                                                           <  1:93798/250‑1 (MQ=255)
                                                                                                                                          tctcctggCCCGCCGGTTCGGTTGGCAGGAAGAAAGCCATATAGACGTCGCTGAGATCGCCCATATCCAGCACCCGACCGCCTGCGGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTGCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGc                                                                                          <  2:400014/243‑1 (MQ=255)
                                                                                                                                          tCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCCTCGACGCGGGGTTGCGCA‑TGCATGatgttagggcggtcctcttatatagcc                                                                                          >  2:163800/1‑226 (MQ=255)
                                                                                                                                          tCAGCGTGCCCGCCTGTTCGGTAGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCCCGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCACTGCGCCGTTCCGTGGCTTG‑TGCCGCTTCCGCGCGGGGTTGAGCC‑CTCATGCTCATGGTG‑CGTGCCGCtttcttagcc                                                                                         >  2:303354/1‑243 (MQ=255)
                                                                                                                                            aGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGCTATTGGTG‑CGTGCCGCTTCTCTGGCCGc                                                                                       >  1:62074/1‑251 (MQ=255)
                                                                                                                                                         gTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATTGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGGGCCGCTTCTATAGCCGCTTTAGAAGcctgt                                                                          >  1:69922/1‑248 (MQ=255)
                                                                                                                                                           tCGGTTGGCAGTAAGAAAGTCATATAGACGTCGCTGAGATCGCCCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACGGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCAGCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATa                                                                         <  1:395701/250‑1 (MQ=255)
                                                                                                                                                             ggTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCT‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCt                                                                      <  1:288543/251‑1 (MQ=255)
                                                                                                                                                              gTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCCGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACCCGGGTTTTCGCCCTGA‑TGATATTG‑TGTCGGGCCGCTTCTTTAGACGCCTTATAAACCGATaactt                                                                     >  2:170349/1‑247 (MQ=255)
                                                                                                                                                                tGGCAGGAAGAAAGGCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCGGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGa                                                                    <  1:207820/250‑1 (MQ=255)
                                                                                                                                                                               cATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGCCCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TTTCGCTTCGAAGCGGGGTTGCGCC‑TGAATGGAATTGGTG‑CGTGCCGCTTCTATAGCCGCCTTTGAAGCCggaaccagagcctttgccgcttacc                                                    >  2:277926/1‑227 (MQ=255)
                                                                                                                                                                                       cGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCt                                            <  1:326863/251‑1 (MQ=255)
                                                                                                                                                                                              gagaTCGCCCATATTCAGCCCCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTGCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGgcg                                      <  2:138601/250‑1 (MQ=255)
                                                                                                                                                                                                      ccATATTGAGCACCCGCCCGCCTGCCGCAGGCCCTTCGCCTGTTGTGGCAACCCGACTGGGCCTGTGTCCGTGAGGCGGGGCTTTCAGTTCGATGGCGTGGATATCTGCGGCAATTCGCTGTTCAGGGGGTTG‑GGGCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑GGTGCCGCTGGTATAGCCGCGTTAGAAGCCGATACCTGAGGTTTCGCCGATTCCAGCGCAGCTCGGGCGCGCTCAgcg                             <  2:229559/251‑1 (MQ=255)
                                                                                                                                                                                                                 ccccGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATc                  <  1:410692/250‑1 (MQ=255)
                                                                                                                                                                                                                 cccagcccgcccgcCCCCCGCACTTCGCCGGGCTTGGTAACCCGATTCGGCCAGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCACCGATATCTGCGGCAAGTCGTCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCTGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATc                  <  2:86654/245‑1 (MQ=255)
                                                                                                                                                                                                                   ccGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGt                <  1:71787/251‑1 (MQ=255)
                                                                                                                                                                                                                      cccGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCAt             <  1:78722/250‑1 (MQ=255)
                                                                                                                                                                                                                        cGCCTGCCGCCAGCACTCCGCCTGGCTTGTCAACCCGATACTGCACGCGGCCGGCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGGCGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATcc           <  1:53300/251‑1 (MQ=255)
                                                                                                                                                                                                                         cccTGCCCCCAGCACCCCGCCGGGTTTGGCAACCCGATCCGGCGCGCGTCCTCCGCGCGGGGCTTTCAGTTCGCTGTCTTCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGGTTGCGCC‑TGAAGGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTGTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCa          <  2:407283/250‑1 (MQ=255)
                                                                                                                                                                                                                                 ccAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TTAATTATATTTGTG‑CGTGCCGCTTCTATAGCCGCTTTTGACGCCGATACCTTAGCTTTCGCCGATTCCAGCGCCGCTCGGGCGCTCTCAGCGGCCGCCCGATCGTCATCCAGCTGtttc  >  1:86804/1‑249 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
CATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCGTGAACTGGGCGACACTGGCGACAAAACTGATGGTTGCAGGAATACGCAGATCTGGCGCGGCATCGAGGATC‑AGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTG‑TGCCGCTTCGACGCGGGTTTGCGCC‑TGAATGATATTGGTG‑CGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGCAGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGC  >  NC_000913/3629679‑3630153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑