Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,947	C→A	intergenic (‑242/+669)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,947	0	C	A	100.0%	98.9 / NA	31	intergenic (‑242/+669)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/20);  total (11/20)

ACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAA  >  NC_000913/3707700‑3708184                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              aCGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCcacaaaca                                                                                                                                                                                                                                            <  1:393285/251‑1 (MQ=255)       tattCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTGTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGAt                                                                                                                                                                                                                                       <  1:390847/250‑1 (MQ=255)                        gATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTGTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAACCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAg                                                                                                                                                                                                                     <  2:115177/251‑1 (MQ=255)                                   ttttAACCCTTTGCAGTGGGTTGTCGCTGCCGGACCTTGTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGTGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgcag                                                                                                                                                                                                           <  2:165015/250‑1 (MQ=255)                                     ttAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgcagca                                                                                                                                                                                                         <  1:163152/250‑1 (MQ=255)                                             ttgtagcgggTTGTAGCTGCATGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGCTGCCGTACTAATTAAAATCAGTGTGACCGTGCGGACGCTCCATAAAATGCCCCTCGTGCACTACAATCTGTCAACAGAATGCGACAACGTAAATACAGCCCACCGTGGTTTACACAAACGGTTAGAATCCACAACCTACGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAttt                                                                                                                                                                                                >  2:445632/8‑251 (MQ=255)                                                  gTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCt                                                                                                                                                                                           >  1:78242/1‑251 (MQ=255)                                                            gCTGCCTGACCGTTTTTGTTTTTTGCCCTGTCGGGTCAACGTTATGATGTGGTGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACAt                                                                                                                                                                                 <  1:101074/251‑1 (MQ=255)                                                               tccTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGAACCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGGGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTTCTACTCCAACATGa                                                                                                                                                                               <  2:379320/249‑1 (MQ=255)                                                               gccggACCTGTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATg                                                                                                                                                                                <  1:423247/245‑1 (MQ=255)                                                                ccTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCACCGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGAcc                                                                                                                                                                             >  1:445902/1‑251 (MQ=255)                                                                  ggACCTTTTTTTTTTTTTGCCCGTGCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTTTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTTCCCCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGATAAATTTGTGCTGCTCCACCATGACCAg                                                                                                                                                                           <  2:179524/250‑1 (MQ=255)                                                                                        ggTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGCGAAATTTGTGCTACTcc                                                                                                                                                                                      >  1:262880/1‑218 (MQ=255)                                                                                           cGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCGTAATATTTAGCAAc                                                                                                                                                  <  1:87206/251‑1 (MQ=255)                                                                                             ggTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACAt                                                                                                                                                <  1:198178/251‑1 (MQ=255)                                                                                                cAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTg                                                                                                                                             >  1:236514/1‑251 (MQ=255)                                                                                                          aGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGt                                                                                                                                   <  1:75087/251‑1 (MQ=255)                                                                                                             gggggATGCCGTACTAATTAACAGCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATGTACTCCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCATAGAAATTTGTGCTACGCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGAATAAGttgt                                                                                                                                <  2:236514/250‑3 (MQ=255)                                                                                                                agATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATACACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACATTCAGCTTAATATTTAGAACCATTTGGTGATTAAGTTTTATg                                                                                                                             >  2:97188/2‑251 (MQ=255)                                                                                                                     ccGTACTAATTAACATCAGTGTAACAGTGCGGATCATCCATAAAATGCACCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTGTTATGCAttt                                                                                                                        <  2:44951/251‑1 (MQ=255)                                                                                                                     ccGTACTAATGAACATCATTGTAACAGTTCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTTTCAACAGAAGGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAAAAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGATCAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCAttt                                                                                                                        <  2:370072/251‑1 (MQ=255)                                                                                                                                   aTCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCt                                                                                                          >  1:284282/1‑251 (MQ=255)                                                                                                                                                    gATCCTCCATAAAATGCCCCTCGTGACCTACAATATGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGatat                                                                                         <  2:108905/251‑1 (MQ=255)                                                                                                                                                              aaaaTGCCCATCGTGACCTACAATCTGTCAACAGAATGTGAAAACATCAATACAGCCAAACGGGATTTACACCAAAGGTGAGAATCCACAAACAACGATTAAAAAAACTTCAAACAGCTATTTGAAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAAAAATAAGCTTAATATTTAGCAACATTTGGTAATAAAGTTTTATGCATTTAATGAAAAAAATCTTAGGAAACGGTGGAAATCTGAGCGAt                                                                                >  1:29785/1‑250 (MQ=255)                                                                                                                                                                    ccccTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATc                                                                                                           >  1:398084/1‑217 (MQ=255)                                                                                                                                                                    ccccTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATc                                                                                                           <  2:398084/217‑1 (MQ=255)                                                                                                                                                                         cGTGACCAACAATCTGTAAAAAGACTGTGAAACCGTCAATACAGACAACCGGGATTTATACCAAAGGTGAGAATCCACAATCAAAGCTTAAAAAAACTTCAAACAGCTATTTGACGCAGAGAACTCTGTGCTAATCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTATTATGCATTTAATGAATAAAATCAGCTGAACAGGTGTCTATCTGAGAGATTAATACCATGa                                                                     >  2:412180/1‑250 (MQ=255)                                                                                                                                                                            gACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCGACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACg                                                                  <  1:44239/250‑1 (MQ=255)                                                                                                                                                                                                        aaCGTCACTACAGCGAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGc                                                                                                                                                                 >  2:193419/1‑127 (MQ=255)                                                                                                                                                                                                        aaCGTCAATACAGCGAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGc                                                                                                                                                                 <  1:193419/127‑1 (MQ=255)                                                                                                                                                                                                                     ccAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTaa                        <  1:381518/251‑1 (MQ=255)                                                                                                                                                                                                                                           gTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAAGCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCaaa  <  2:299957/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              ACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAA  >  NC_000913/3707700‑3708184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑