Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	68.8 / NA	24	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/11);  total (13/11)

AACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCG  >  NC_000913/1306497‑1306962                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                   aaCTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGc                                                                                                                                                                                                                         >  1:224195/1‑251 (MQ=255)     ggagACCTATGATGAGGTCTACCCAAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCAGATCTGGAGCAGAAAAAAACCATACAGTTACTGGGAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTGCCGTAACAGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGCGTTTCAGACGCGCCGTTTCTTGCCTg                                                                                                                                                                                                                      >  2:23907/4‑250 (MQ=255)               gatgaGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCACCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGATAATGGAAGGGGAATTACCGTCGCCGATCAACCCGCATTCCGGATGAGTTTTGCGTACCCGTTGCCAGATTGCCGGTCCAGAGTGC‑GCCAAAAAACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATcc                                                                                                                                                                                                           >  1:19900/1‑251 (MQ=255)                    ggTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCttt                                                                                                                                                                                                      >  1:330545/1‑251 (MQ=255)                                           cTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCTGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGtt                                                                                                                                                                               >  1:466796/1‑251 (MQ=255)                                                                                   cccGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCg                                                                                                                                       <  1:310810/250‑1 (MQ=255)                                                                                           gggAGAAAAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatga                                                                                                                               <  1:317899/250‑1 (MQ=255)                                                                                                             cccAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGAt                                                                                                             <  1:387366/250‑1 (MQ=255)                                                                                                                   tACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCttttt                                                                                                       >  1:114132/1‑251 (MQ=255)                                                                                                                         aaGGGGAATTACCGACGCCGATCAACCAGCAATCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGACGGTCCAGAGTGC‑GCAAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGATTCTTGACTGAAAGGCGATCCGCTTTAAAAACAATAAGGGCTGAAAGTTGTCAACACTTTTTCACACTAAAAGCGATTATTTATTCCCGCCAGATGATATGACAACATTTGTGCTCTTTTTCGCGGc                                                                                                 >  1:356682/1‑251 (MQ=255)                                                                                                                                       tCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCCTAACAGAATGCgg                                                                                   >  1:174671/1‑251 (MQ=255)                                                                                                                                                aaCCCGCCTTCCGTTTGTGTTTTCCGTCCCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACa                                                                          <  1:46085/251‑1 (MQ=255)                                                                                                                                                 gcccGCCTCCCGGTTGTGGTTTCCGCACCCTTGGCCCGATTTCCGGCCCATA‑TGCCGCCAAACCACGTCCTGTGTGGGAGGGGAGTTCCAGACACGGCGTTGCGTGCCTGAAATGCGATCCGCTTTAAAAACAATAAGGGTTGACAGTTGTCAGTCCTGGTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTGTTGCGTGGTATAACAGAATGCGTGCaaaa                                                                             <  2:278610/246‑1 (MQ=255)                                                                                                                                                  cccGCCTTCCGGTTGTGTTTTCCGTACCCGTAGCCCGATTACCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGCGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAATGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCCCCAGATGATATGGCAAAGTTTGCGATCTTTTTCACGGCATAACAGAAAGAGGGCCAAAAAATc                                                                        >  2:335144/1‑251 (MQ=255)                                                                                                                                                     gCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATTCGGGCAAAAACATCGtt                                                                     >  2:174640/1‑251 (MQ=255)                                                                                                                                                                  tttttCCGTCCCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATaa                                                                                                                                                                                           <  2:466821/119‑1 (MQ=255)                                                                                                                                                                  gtTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATaa                                                                                                                                                                                           >  1:466821/1‑120 (MQ=255)                                                                                                                                                                  gtTTTCCGCACCTGTTGCCCTATTGCCGGTCCTGAGTGC‑GCCAAAACCCGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGGTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCCCCATCt                                                        <  1:295908/251‑1 (MQ=255)                                                                                                                                                                           aCCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTc                                                                                                                                                                            <  1:457283/126‑1 (MQ=255)                                                                                                                                                                           aCCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTc                                                                                                                                                                            >  2:457283/1‑126 (MQ=255)                                                                                                                                                                           aCCCGTTGCCCGATTGCCGGTCCAGAGGGC‑GCCAAAACCCGTCCTGTTTTGGATGGGAGTTTCAGCCACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTcc                                               <  2:330545/251‑1 (MQ=255)                                                                                                                                                                                            cGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGCCCAATcaccac                              >  2:135560/1‑251 (MQ=255)                                                                                                                                                                                                                   cGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTc        <  2:174671/251‑1 (MQ=255)                                                                                                                                                                                                                         gttcgggcggggAGTTCCATACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGCCCGCTTTTTTCCACGCTAAAAGCGATTATTTATTCCCGCTAGATGATATGGCACAGTTTGTGATCTTTTTCGCGGCCTAACAGAATGCGGGCAAAAACACCGTTGATTTCACCATCTTCACTGGCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCg  <  1:280222/243‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                   AACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGC‑GCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCG  >  NC_000913/1306497‑1306962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑