| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 92,843 | A→G | P477P (CCA→CCG) | ftsI → | peptidoglycan DD‑transpeptidase FtsI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 92,843 | 0 | A | G | 93.2% | 164.8 / ‑1.7 | 59 | P477P (CCA→CCG) | ftsI | peptidoglycan DD‑transpeptidase FtsI |
| Reads supporting (aligned to +/- strand): ref base A (4/0); new base G (30/25); total (34/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-01 | |||||||||||
AACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCCATCAT > NC_000913/92599‑93073 | aaCGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTAAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGgg‑cggc > 2:414171/1‑251 (MQ=255) gaagtagCTTATGTCCTCAACAACAACGGTGGACTGACATAGAGAGGGCCACATTCTCTTTCGGCTACGAGCTAATGGTCACACCGTTACAGTTAGAGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCCAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGACCGCACCGAGGTGCACATGATGGCAAACGAGGCGCGACCAgcgggggac > 2:452830/7‑243 (MQ=255) tataTCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGATACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGa > 1:113239/1‑249 (MQ=255) ccTCAAAAACAACGGTGGTCTGAAATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAggcgg < 1:96474/250‑1 (MQ=255) cacAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCGTCCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCGCCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGgcggcg < 2:158866/249‑1 (MQ=255) aaaaCAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTa < 1:41040/251‑1 (MQ=255) aaaCAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCCACTATCGGCAGCTACGGCCTTTATCGCCCCCTGTCGCTTACCAAAGTTCACCCCCCGGTTCCCGGTGACCGTGTCTTCCCGGAATCCATTGTCCGCCCCGTGGGGCATTTGATGGAAAGCGGGGCGCTACCGGG‑ATGCGGCGGCGTGAcggcggggctta > 2:90969/1‑244 (MQ=255) acGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACAACGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGACCGTGTCTTCCCGGAATCCATTGACCGCACCGTGGTGCCTATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCt > 2:446989/1‑251 (MQ=255) ggtCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgt > 1:364789/1‑251 (MQ=255) atagGGCCACCCTCTCTTTCGGCTACGGGCTAATGGTAACCCCGTTACAGTTAGCGCGAGTCTACGCAACTATCGTCAGCTACGGCATTTAGCGCCCACTGGCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaaa < 2:352400/249‑1 (MQ=255) gagGGCCACCTTCTCTTTCGGCTACGCGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTGCCCGGTGAACGTGTCTGCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaaaa < 1:19411/251‑1 (MQ=255) gCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTATACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGCCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCgg > 2:406496/1‑251 (MQ=255) ccACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGAGAGTCTAAGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCACGGTGAAAGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGAGCCTATGATGGAAAGCCTGGCGCT‑CCAGGGAGGCGGCGGCGTGAAGGCGGCGATTAAAGGAGACCGTATCACCCTTAAAAcagg > 2:133663/1‑247 (MQ=255) cccTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAc < 1:414171/250‑1 (MQ=255) gCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTc < 2:362128/251‑1 (MQ=255) cTACGGGCTACTGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAGGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAGAGGCTATCGTATCGCCCTTAAAACCGGTAC‑C‑GCGAAACAGGTCg > 2:4484/1‑251 (MQ=255) cTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCg > 1:314107/1‑251 (MQ=255) gggCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGcc < 1:363148/251‑1 (MQ=255) gggCTAAGTGTAACACCGGTACTGTTAGCGCGAGGCTACGCAACTATCGTCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGcc < 2:20532/251‑1 (MQ=255) gCTAATGGTAACAACGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTCTCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCACGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGAATAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑A‑GCGAAAAAGGTCGGGCCgg > 1:62683/1‑251 (MQ=255) tAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGCTTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGGGGTGCATATGATGGAAAGAGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTAGCGTATCGCCATTAAAACCGG‑AC‑CAGCGAAAAAGGGCGGGCCGGAc > 2:1803/1‑251 (MQ=255) tAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGCATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTCAAACCGGTAC‑C‑GCGGAACAGGGCGGGCCgtcc > 2:206291/1‑248 (MQ=255) tGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCACACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGGGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGGGGCGCTGCCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGCAC‑C‑GCGAAAAAgggcgggcagggaggg > 2:175186/1‑243 (MQ=255) aaCACCGTTTCAGTTAGCGCGAGTCTACGCAACTACCGGCAGCTACGGCATTTATCGCCCACTGTCGATTTCCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAACCCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCt < 2:354930/251‑1 (MQ=255) aaCACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGc < 1:160286/250‑1 (MQ=255) acacCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAATTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGGGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCTGCGATTAAAGGCTATCGTATCGCCATTAAACCCGGTAC‑C‑GCGAAAAAGGTAGGGCCCGACgggccctc > 2:179159/1‑245 (MQ=255) caccgtttcaggtAGCGCGAGTCTACGCACCTATCGGCAGCTACGGCAGTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTGCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGGGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGTTCGGGCCGGACGGGCGCTAc < 2:416168/239‑1 (MQ=255) ccgttACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACAt < 1:203751/248‑1 (MQ=255) gTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGAATTTATCGCACACTGGCGATTACCACAGTTGGCCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAATGCGTGGCGCTACAGGG‑CGGAGACGGCGTGGAGGCGGCGATTAAGGGCTATCGTATCGCCATTAGAACCGGTACAC‑G‑GAAAAAGGTCGGAACGGAAGGTCGGTACCTCaataaat > 2:406084/1‑251 (MQ=255) tAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGGCGGGCCGGACGGTCGCTACATCAATAAatat > 2:98664/1‑251 (MQ=255) cTATCGGCAGCTACGGCATTTATCGACCACTGTCGATTACCACAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCTCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGc > 1:133950/1‑251 (MQ=255) cTACGGCATTTATCGCCCACTGTCGATTCCCAAAGTTGACCCCCCGGTTCCCGGTGCACGTGTCTTCCCGGAATCCATTGTCCGCCCCGTGGTGCGTATGATGGAAAGCGTGGCGCTACCGGA‑CGGCGCCGGCGTGAAGGCGGCGATTAACGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGACCACGGTCGAGCCGGACGGTCGTTACATCATTATAAACATTGATTATACCGCAGGCGTTGCGCCTg > 2:107860/1‑251 (MQ=255) tACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCg > 2:38795/1‑193 (MQ=255) tACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCg < 1:38795/193‑1 (MQ=255) cATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCTCTACCGGT‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCAGTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTc < 1:305758/251‑1 (MQ=255) aCTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAAACCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTcg > 2:468299/1‑251 (MQ=255) atGTCGATTACCACAGTTGACCCCCCGGATCCCGCGGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCACATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTACAGGCTATCGAATCGCCATTAGAACCGGAAC‑C‑GCGTGACGGGTAGGGCCCGACGGGGGCTACATCCGTAAATATATAGGTTAAAACGCAGGCCATGCGccgtggggtccacagcacgtcac > 2:259890/2‑230 (MQ=255) gTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCgcgc < 1:243068/251‑1 (MQ=255) aaaGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGgttgttgt > 1:436574/1‑251 (MQ=255) cccGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATc < 1:361780/251‑1 (MQ=255) ccGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATcc > 1:144835/1‑251 (MQ=255) cGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTTTCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTTCTTATCCCGCAGGCGTTGCGCCTGCGCGTCCGCCGCGCATCGCGCTGGTTGTTGTTATCAACGATCCg > 2:111370/1‑251 (MQ=255) tCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAgg > 1:283990/1‑251 (MQ=255) ggTGAACGTGTCTTCCCGGAATCCATTGTCCGCACCGGGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCggg < 1:4484/251‑1 (MQ=255) gtCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTgc < 2:24739/185‑1 (MQ=255) gtCTTCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTgc > 1:24739/1‑185 (MQ=255) tCCCGGAATCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGTCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAAtactac < 1:198396/248‑1 (MQ=255) cGGAAGCCAGTGGCCGCACCGTGGGGAATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGTCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTAGACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACggcggc < 2:174636/251‑1 (MQ=255) gAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTAGCGCTACCAGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAATGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGACAGCCGCGCTTCGCGCTGGTTGTTGTTTTCAACGATCCGCAGGCggggacataccaacgccgggg > 2:338162/1‑233 (MQ=255) aaTCCATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGcc < 1:206291/251‑1 (MQ=255) cATTGTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGACTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTaaaatcttcggaggagcccgtt > 1:19009/1‑232 (MQ=255) gTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAAttataaggagggggcgttttccg > 1:405883/1‑229 (MQ=255) gTCCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCCGTTTCcgc < 1:394048/251‑1 (MQ=255) ggcaaaggtgggAAATCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTAACGCCATTAAAACCGGTCC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGTAGGCGGGTAAATACTACGGCGGCGTCGTTTCCGCGCGGGTCTTTGGt < 2:58602/241‑1 (MQ=255) atatGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGAAAAACTACGGCGGGGCCCGTTCCACGCCCGTCttttgggcc > 1:347024/1‑245 (MQ=255) tatGATGGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACCGCGGCGCCGGTTTCTCGCCgggcgtttgggacc > 1:458412/1‑239 (MQ=255) atgatgGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCca < 1:98664/250‑1 (MQ=255) gatgGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGAAATACCACGCCGGCGCCGGTTCCGCGCCgggcgtttggggcatcc > 1:194240/1‑236 (MQ=255) atgGAAAGCGTGGCGCTACCGGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCCGGTTTCGCGCCGGTCttttggtcgctcat > 1:288161/1‑240 (MQ=255) | AACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGG‑CGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTAC‑C‑GCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCCATCAT > NC_000913/92599‑93073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |