Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 91,904 G→A E164E (GAG→GAA)  ftsI → peptidoglycan DD‑transpeptidase FtsI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091391,9040GA92.3% 102.2 / 1.1 39E164E (GAG→GAA) ftsIpeptidoglycan DD‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (15/21);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

GAGCGTGCCGGTAAAAGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGG‑CTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCG  >  NC_000913/91664‑92149
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     
gggCGTGCCGGTAAAAGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGG‑CTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGtt                                                                                                                                                                                                                                             <  2:13200/249‑1 (MQ=255)
         ggTAAAAGCGATTGGGGCTGCCCCTAAAGAAGGGCAGGGCGTTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGG‑CTACCGCGCGCAATATTCCGCTGGAGCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAATAACTGAAACTGCCGGGGTGTCATCTGCGTGAAGAATCTCGCCGTTATTAtccat                                                                                                                                                                                                                                    <  2:121601/251‑3 (MQ=255)
                                  aaataagTGCATGAAGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGG‑CTACCGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAACGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCAt                                                                                                                                                                                                           <  2:368017/247‑1 (MQ=255)
                                     gaagTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGG‑CTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCAttgg                                                                                                                                                                                                        >  1:82771/1‑248 (MQ=255)
                                         ggcactccGCTGGCGGTATCAGCGTCGGTGACAGCTGGAAGGCGCTGG‑CTCACTCTCTCAATATCCCGCTGGATCAGCTTTCCGCCCGCATTAACGCCAACCCGAAAGGTCGTTGTATTTATCTGGCGCGTCAGTTGACCCCTGACATTGCTGACTCCATCAAAAAAATGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCAGCCGGCGAAGTGACTGCTCACCTTATTGGCttt                                                                                                                                                                                                    <  2:190604/244‑1 (MQ=255)
                                                   gtgtgtttgcagagccGGTGACCGCTGGAAGGCG‑TGGTCTCACGCGCTCAATATTCCGCTGGTTCAGCTTTCATACCGTATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGGCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCCTCCGGCGAAGTGACTGCGCTCCTCATTGGCTTTACTAACGTc                                                                                                                                                                                           <  2:221609/235‑1 (MQ=255)
                                                                          gCTGGAAGGCGCTGG‑CTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCAGTAACGCCAACCCGAAAGGGCGCTTTATGTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACGGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGtt                                                                                                                                                                   <  2:461516/251‑1 (MQ=255)
                                                                            gggAAGGCGCTTG‑CTAACGCGCTCAATATTCCGCTTGATCAGCTTTCAGCCCTCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTCACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCTTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGTTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTga                                                                                                                                                                 <  1:420124/250‑1 (MQ=255)
                                                                               aaGGCGCTGG‑CTAACGAGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGACGTTCTTATCCATCCGGCGAAGTGACAGCTCTCCTCATTGGCTTTACTAACGTCGTTAGTCCAGGGATTGAGGGCGTTgaga                                                                                                                                                               >  1:163090/1‑250 (MQ=255)
                                                                                            aaCGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGAt                                                                                                                                                    <  1:337149/249‑1 (MQ=255)
                                                                                                  cTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCt                                                                                                                                            >  1:362644/1‑251 (MQ=255)
                                                                                                   tCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCAGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATc                                                                                                                                                                                                          >  2:66756/1‑188 (MQ=255)
                                                                                                   tCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCAGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATc                                                                                                                                                                                                          <  1:66756/188‑1 (MQ=255)
                                                                                                    cAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGCCTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATACATGGCtt                                                                                                                                           >  2:42944/1‑250 (MQ=255)
                                                                                                     actatTCCGCTGGAACAGCTTTCAGCCCGCATTAAGGCCAAACCGTCAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCAGTTATTATCCATCCGGCGAAATGACTGCTCACCTCATTGGCTTACCTACCGCCGATAGTCAAGGGGTTGAGGGCGTTCagaagtatatcgataacaggctaa                                                                                                                                          >  2:161372/3‑231 (MQ=255)
                                                                                                           ccGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCa                                                                                                                                   <  1:28649/251‑1 (MQ=255)
                                                                                                                           cAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCAtt                                                                                                                   >  1:199488/1‑251 (MQ=255)
                                                                                                                           cAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTAATTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTAAATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTAGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGACTTACCGGGCAGCCGGGTGCGGGCAtt                                                                                                                   >  2:314244/1‑251 (MQ=255)
                                                                                                                            aGCCCGCATTAACACCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGTGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAATAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATtg                                                                                                                  <  1:204362/251‑1 (MQ=255)
                                                                                                                              cccGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGGCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATtgtg                                                                                                                <  1:215398/251‑1 (MQ=255)
                                                                                                                                  cATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTa                                                                                                            <  1:176689/251‑1 (MQ=255)
                                                                                                                                      aaCGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTaa                                                                                                           >  1:59638/1‑248 (MQ=255)
                                                                                                                                                 gAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTc                                                                                             >  1:397548/1‑251 (MQ=255)
                                                                                                                                                            ttatttatCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGa                                                                                   <  1:42944/250‑1 (MQ=255)
                                                                                                                                                                             cAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGCCTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTg                                                                  >  2:272427/1‑250 (MQ=255)
                                                                                                                                                                                 tGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGc                                                             >  1:426016/1‑251 (MQ=255)
                                                                                                                                                                                 acgctcttccgatctcGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGAc                                                                                                       >  1:361199/16‑209 (MQ=255)
                                                                                                                                                                                 acgctcttccgatctcGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGAc                                                                                                       <  2:361199/194‑1 (MQ=255)
                                                                                                                                                                                    aCCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAg                                                          <  1:195099/251‑1 (MQ=255)
                                                                                                                                                                                         gACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGctg                                                      <  1:51037/250‑3 (MQ=255)
                                                                                                                                                                                               gCGGAATACATCAAACAACTGAAACCGCCGGGGTTTCATCTGCGTGAAGAATCTCTCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTTCTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACaa                                               <  2:121433/251‑1 (MQ=255)
                                                                                                                                                                                                   aCTACATCAAAAAACGGAAACTGCCGGGGATTCATCTGCGGGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAAGTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTg                                           <  2:397548/251‑1 (MQ=255)
                                                                                                                                                                                                                    aaactgCCGGGGATTCATCTGCGTGAAGAATCGCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCCCAACCTGGCGCTGAGTATtgatga                          <  2:355140/251‑1 (MQ=255)
                                                                                                                                                                                                                      actgCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGCGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATtgatgacc                        >  2:22779/1‑249 (MQ=255)
                                                                                                                                                                                                                         gCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATACATGGCTTACCGGGCAACCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTAGCGTAATTGAAGATATTTCTTCAACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGcc                     >  2:251837/1‑251 (MQ=255)
                                                                                                                                                                                                                         gCCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACg                       <  2:603/249‑1 (MQ=255)
                                                                                                                                                                                                                                  tCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGgcgc            >  1:73602/1‑251 (MQ=255)
                                                                                                                                                                                                                                        gCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGATTATTGATGAACGCCTGCAGGCGCTGGttt      <  1:381720/251‑1 (MQ=255)
                                                                                                                                                                                                                                          gTGAAGAATCTGGCCGTTATTATCCATCCGGCGAAGTGACTGCTAGCCGCAATGGCTTTACTAACGGCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGGCAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTAt    <  2:425796/251‑1 (MQ=255)
                                                                                                                                                                                                                                            gaagaaTCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATcg  >  1:1269/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     
GAGCGTGCCGGTAAAAGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGG‑CTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCG  >  NC_000913/91664‑92149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑