Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	76,700	A→G	D200D (GAT→GAC)	sgrR ←	DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	76,700	0	A	G	97.9%	139.0 / NA	49	D200D (GAT→GAC)	sgrR	DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base G (18/29);  minor base T (0/1);  total (19/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCA  >  NC_000913/76459‑76935                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            ggttatttggttgtgtGCGGATCACCGCATACGGTCCTGTGCCGATGGGATGGCTGGCAAAGGTACTGAGGTTTTACCATTCGCGCGGCAGGATCATCGCCGGAACTTGCACCAGCCATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGTCGATCACGTCGTcc                                                                                                                                                                                                                                       <  2:430590/233‑1 (MQ=255)    gATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTcc                                                                                                                                                                                                                                 <  1:274075/251‑1 (MQ=255)             tGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACCCAATGTCAGCAATATGCGAGTAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACg                                                                                                                                                                                                                         >  2:419916/1‑250 (MQ=255)                 gcggaTCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCAt                                                                                                                                                                                                                    >  2:159434/5‑251 (MQ=255)                   ggaTCACCGCATACGGTCCGGTGCCGATGGGATAGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGGCCATTTCCAGTTCACGACCATgg                                                                                                                                                                                                                  >  1:390768/3‑251 (MQ=255)                    gaTCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATgat                                                                                                                                                                                                                 >  1:437594/2‑249 (MQ=255)                     aTCACCGCATACGGACCGGGGCCTAGGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGTTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAAGGTCAGCAATATGCGAATAGAGCGGCTGCGTTGTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATgatg                                                                                                                                                                                                                <  1:456780/251‑4 (MQ=255)                             aTACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGa                                                                                                                                                                                                        <  1:55675/251‑1 (MQ=255)                               aCGGACCGGTGCCGGTGGTATTGCTGGCAAAGTTACTGAGGGTTTCCCAATCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACTTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACt                                                                                                                                                                                                      <  1:22310/251‑1 (MQ=255)                                     cGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGa                                                                                                                                                                                                <  1:15395/251‑1 (MQ=255)                                           cGATGGGATGTCTGTAAAAGTTACTGTGGGTATCCCCTTCGCGGTGCAGGAAACTTGCCAGATCTTGCCCCTGCATTAACTGTAACCAGCGGTCCGGTTGCGTGATATGGATATCCAGCGTCCAGGGCGTCGGCGACACATTTTCAGCAATCTGCGAATAGATCGTCAGCGTATTGATTCGTTTTAAAGAGGCGATCTCGTCGTCCATTTCCAGTTCACGACCATGATGAAAATTGAcctttgtacgcaaa                                                                                                                                                                                          <  2:358390/251‑14 (MQ=255)                                             atgggatggCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGCTTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCaaaa                                                                                                                                                                                         >  1:3380/1‑250 (MQ=255)                                               gggatggCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGa                                                                                                                                                                                      <  1:279018/251‑1 (MQ=255)                                                  atggCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCCGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGCTATCCAGCGCCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGAGATCCCGTCGTCCCTTTCCAGTTCACGACCATAATGAAAAAGGAATCCTTGACGCAAAAAGGAAc                                                                                                                                                                                   >  2:331804/1‑251 (MQ=255)                                                                   tGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGat                                                                                                                                                                  >  1:361705/1‑250 (MQ=255)                                                                     aGGGTTTCCCATTCACGCGGCAGGATCATCGCCCGAACTTGCCCCAGCAATAACGGTAACCAGCGTTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGCCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGTACCCGTCGACCGTTTCACGTTCACGACCTTGATTAAAATGGAATACTGGACGCAAACAGCAAAGCCAGTGAAGCggagtaat                                                                                                                                                                >  2:391418/1‑245 (MQ=255)                                                                            cccATTCGCGCGGCAGGATCATCGCCGAAACCTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGGATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGACAGTGAAGCGGAGATATTtgctgc                                                                                                                                                         >  1:130720/1‑251 (MQ=255)                                                                             ccATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTATCCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGcc                                                                                                                                                        <  1:470950/251‑1 (MQ=255)                                                                                 gcgcgcgGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAgt                                                                                                                                                     <  1:419916/249‑1 (MQ=255)                                                                                     gcgGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGtggtgg                                                                                                                                                <  2:361705/251‑1 (MQ=255)                                                                                     gcgGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGtggtgg                                                                                                                                                <  1:393026/251‑1 (MQ=255)                                                                                         cAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACCCAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTTGGCg                                                                                                                                             >  2:283805/1‑250 (MQ=255)                                                                                               caccgccgaaccTTGCCCCATCAATAACGGGAACCAGCGGTCCTGTTGCTTGCGATGGATATCCAGCGTCCAGGGCGTCGGCGACTCAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTGTAAAGAGGCGATCACGTCGTCCATTTCCAGAGCTCGACCATGATGAAAATGGTCGTCTGTACGCAAAAAGAAACGCCGGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTg                                                                                                                                      <  2:281737/240‑1 (MQ=255)                                                                                                      ggAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGAACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGAGGTCTGCTTcc                                                                                                                                 >  2:183770/1‑249 (MQ=255)                                                                                                        aaCTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTc                                                                                                                                                                                                     >  1:289776/1‑179 (MQ=255)                                                                                                        aaCTTGCCCCAGCAAGAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCATCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTc                                                                                                                                                                                                     <  2:289776/179‑1 (MQ=255)                                                                                                              ccccAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCAt                                                                                                                       <  1:159434/251‑1 (MQ=255)                                                                                                                ccAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCAttt                                                                                                                     <  1:294943/251‑1 (MQ=255)                                                                                                                  agcaaTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTc                                                                                                                   >  2:178345/1‑251 (MQ=255)                                                                                                                     aatAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTc                                                                                                                <  1:377362/249‑1 (MQ=255)                                                                                                                              aaCCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATgcg                                                                                                       >  1:94464/1‑251 (MQ=255)                                                                                                                              aaCCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATgcg                                                                                                       <  1:333349/251‑1 (MQ=255)                                                                                                                                                gTGAGATGTATATCCAGCGTCCAGGTCGTCGGCGACACAATGACAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGAt                                                                                     <  1:251846/251‑1 (MQ=255)                                                                                                                                                       ggATATCCAGCGGCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCgg                                                                              <  1:346208/251‑1 (MQ=255)                                                                                                                                                                    tccgggCGTCGGCGACACAATGTCCGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCTTGTCGGCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAATAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATGTATGCGCGTTAGCGAACTGATTATTTGCCGGGCTATATGGGtt                                                                  <  2:339837/247‑1 (MQ=255)                                                                                                                                                                    ccAGGGCGTCGGCGACACAATGTCAGCAATATGCGCATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCCGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTACTTCCAGTTCCCCATTTTCCTCCTTTATGCGCGTTAGCGACCTGAAGATTTGCCGGGCAATATGGGttt                                                                 >  2:361037/1‑251 (MQ=255)                                                                                                                                                                     cagggcgtagtgcacctcaggtaaGCAATATGCGAATAGAGCGGCAACGTATTGATTCTTTTTAAAGAGGCGACCACGTCGTTCATTCCCAGATCACGACCATGATGAAAATTGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTTCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCCTTTTCCTCATTGATGCGCGTTAGCAAACTGAAGATTTGCCGGCCAATATGGGttgc                                                                <  2:409853/228‑3 (MQ=255)                                                                                                                                                                      aggtcGTCGGCGAAATAATGTAAGCAATATGGGAATAGGGCGGCCGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAATAAACGCCAGTGAATCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTGGCCTGGCAATTTGGGTTTCg                                                               <  2:209247/247‑1 (MQ=255)                                                                                                                                                                           gTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCAATATGGGTTTCGGAACg                                                          <  1:285955/251‑1 (MQ=255)                                                                                                                                                                                       aTGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGGTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGGCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGTCGGGCAATAGGGGTTGCGGAACGGCGCAATGCGCt                                              <  1:363977/251‑1 (MQ=255)                                                                                                                                                                                            aGCAATATGCGCATAGAGCGGCAGCGTATTGATTAGTTTTAACGAGGCGATCACGTCGTCCATTTCCAGTTCACGCCCATGATGAAAATGGTCTCCTGGACGCACAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGCTTTGCCGGGCAATATGGGTTTCGGCACGGCGCACTGCGCTGcc                                           >  2:64232/1‑249 (MQ=255)                                                                                                                                                                                             gCAATATGCGCATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAATTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAAGTCGCTGCCAgg                                        >  2:440658/1‑251 (MQ=255)                                                                                                                                                                                             gCAATATGCGAATAGAGCGGCAGCTGATTGATGAGTTTTAAAGAGGCGATCCCGTCGGCCAGTGCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGcctgg                                        <  2:3380/251‑4 (MQ=255)                                                                                                                                                                                               aaTATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTa                                      <  1:112855/251‑1 (MQ=255)                                                                                                                                                                                                       aaTAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACGTCGTCCATTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGAtt                               >  1:75692/1‑250 (MQ=255)                                                                                                                                                                                                                          gattgggtttAAAGAGGCGTTCACGTCGTCCTATGCCAGTTTACGACCATGATGAAAAGGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGGGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCAATATGGGTGTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTaga           <  2:306916/244‑1 (MQ=255)                                                                                                                                                                                                                             tgtttttAAAGAGGCGATCACGTCGTCCTGTTCCAGTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGc         <  2:345590/247‑1 (MQ=255)                                                                                                                                                                                                                                gtttAAAGAGGCGATCAAGTCGTCAATTTCAAGTTCACGACCATGAGGAAAATGGACTCCTGGGCGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCACGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTGGCGAACGGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCGAGGTAGCAGCTAACGCAACGGACGATAATAGAGCAcgc     >  2:231564/2‑251 (MQ=255)                                                                                                                                                                                                                                    aaaGAGGCGATCACGTCGTCCATTTCCATTTCACGACCATGATGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGATATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGTCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCa  <  1:168711/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            GTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGGATATCCAGCGTCCAGGGCGTCGGCGACACAATGTCAGCAATATGCGAATAGAGCGGCAGCGTATTGATTCGTTTTAAAGAGGCGATCACATCGTCCATTTCCAGTTCACGACCATGGTGAAAATGGACTCCTGGACGCAAAAAGAAACGCCAGTGAAGCGGTGAAATTTGCTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCATTTATGCGCGTTAGCGAACTGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCA  >  NC_000913/76459‑76935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑