Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,038	G→A	H97Y (CAT→TAT)	umpH ←	UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,038	0	G	A	93.8%	139.9 / 1.0	48	H97Y (CAT→TAT)	umpH	UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (22/23);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

GGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCAT  >  NC_000913/699792‑700284                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       ggcggggctTACCAACATAGATCGGTTTGCGCCCTGAGATTTGCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATc                                                                                                                                                                                                                                                    <  2:50381/247‑1 (MQ=255)           aCCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGAGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCtt                                                                                                                                                                                                                                          <  1:213940/251‑1 (MQ=255)            ccAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTc                                                                                                                                                                                                                                         <  2:465591/251‑1 (MQ=255)             cAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCCGGGTTCACATCGGTAATAGGGAAACCGGCTTTGTACAGTTCATAATTCAGTACGCCtt                                                                                                                                                                                                                                          >  2:297393/1‑249 (MQ=255)             cAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCg                                                                                                                                                                                                                                        <  1:78262/251‑1 (MQ=255)                                       aTTTTCTCAATCCCTGCACACAACGAGCCACAAGCGGGATAAAAACCGCGCCCGTAGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTc                                                                                                                                                                                                              >  1:392275/1‑251 (MQ=255)                                              caagcccTGCACACAACGCGCCACATGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGc                                                                                                                                                                                                         <  2:322890/245‑1 (MQ=255)                                                             aCGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCa                                                                                                                                                                                        <  1:225960/251‑1 (MQ=255)                                                               gcgcCACAAGCGTGATAAAAACCGCGCCCGTGGGTGTACGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGTGTTCACATCTGTAATAGTGAAACCGTCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGt                                                                                                                                                                                      <  1:63083/251‑1 (MQ=255)                                                                  cctcaAGCGGTATAAAAGCCGCGCCCGTGGGTGTCCGGATGTGTGGCGATAAATCGTGCACCGTTAGCGAGGAAATAGGCTGCTTTATGCATCATGTCCCAGTGTTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGTTTTGTACAGTTCATAAATCAGTGCGCCTACGCCCCCCACATACGCGTTCTTGCCTTCCTGGCGACGCAGGAAATCTGCAGTCGc                                                                                                                                                                                   <  2:14713/248‑1 (MQ=255)                                                                  ccACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGc                                                                                                                                                                                   >  1:161571/1‑251 (MQ=255)                                                                   cacaAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGAAGGAAATCGGCAGTCGcc                                                                                                                                                                                  <  1:43217/251‑1 (MQ=255)                                                                      aaGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATc                                                                                                                                                                               <  1:187137/251‑1 (MQ=255)                                                                            gATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCagag                                                                                                                                                                         <  1:300621/251‑1 (MQ=255)                                                                                 aaaCCGCGCCGGTGGGTGTCCGAATTGATAGCGATTAAACGTGCACCGTTAGCGACGAAATAGGCTCCTTTATGCATCCTGTACCAGTTCTAGGAACGCGTTTCGCAAACAATAACGCAATCAGGATTCTCATCGGTAATAGCGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTAGCCCACCACATACGCTTTCTCACAATACTAGCGACGCAGGAAAGCGGCAGACGCCCTCGCACAGGtac                                                                                                                                                                     >  2:192929/1‑249 (MQ=255)                                                                                       cgcCCGTGGGTGTACGGATTGGGTGCGATAAAACGTGCACCGTTAGCGATGAAATAGTCTACTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAAGCACGAAAGCAGGGTCCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCGTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAacac                                                                                                                                                              <  2:197693/251‑1 (MQ=255)                                                                                        gcCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGCCGCAGGAAATCGGCAGTCGCCATCGCAGAGGAATAAAACACg                                                                                                                                                             >  1:86905/1‑251 (MQ=255)                                                                                          ccGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGAGTTTCGCCAACAATCACGAAATCAGGTTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCt                                                                                                                                                           <  1:67240/251‑1 (MQ=255)                                                                                            gTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAAAAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGCAATCGGCAGTCGCCATCGCAGAGGTATAAACAACGCTGt                                                                                                                                                         >  2:118434/1‑251 (MQ=255)                                                                                              gggggtCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGAAGTCGCCATCGCAGAGGTATAAAACACGCTGTc                                                                                                                                                        <  1:254460/246‑1 (MQ=255)                                                                                              gggTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCcaccac                                                                                                                                                                                                                                >  1:470496/1‑178 (MQ=255)                                                                                              gggTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCAGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCcaccac                                                                                                                                                                                                                                <  2:470496/178‑1 (MQ=255)                                                                                                       gATTGGTGGCGATAAAACGTACACCGTTAGCGAAGCAATAGGCTGCTTTATGCATCATGTCCCAGTTGTCGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTACTAGTGAAACCGGCTTTGTACCGTTCATAAATCAGTGCGCCTTCGCCAACCACATACGCTTTCTTGCCTTCCTGGCGACGAAGGAAATCGGCCGTCGCCACCGCAGAGGTATAAAACACGCTGTCAGGGACATCg                                                                                                                                              >  2:463371/1‑251 (MQ=255)                                                                                                              ggCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCt                                                                                                                                        <  1:459030/250‑1 (MQ=255)                                                                                                                               cGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTc                                                                                                                      <  1:253323/251‑1 (MQ=255)                                                                                                                               cGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGGTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTc                                                                                                                      <  1:312884/251‑1 (MQ=255)                                                                                                                                        cgaAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTCCATCGACACCTGCGGGGGCAAAGCCGTTCGCCAGATCt                                                                                                             >  2:9936/1‑251 (MQ=255)                                                                                                                                           aaTAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGc                                                                                                          <  1:287152/251‑1 (MQ=255)                                                                                                                                                    ctTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGc                                                                                                 >  2:442980/1‑251 (MQ=255)                                                                                                                                                     tttATGCATACTGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTAAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCACCGCTGGAAATCGGCCGTCGCCATCGCAGAGGAATAAAACACGCTGGAAGGAAAATCGACACCTGCGGAGGCAACGCGGTTAGCCAGATCTTGCCCAGTCTGCg                                                                                                >  2:373452/1‑251 (MQ=255)                                                                                                                                                      ttATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCg                                                                                                <  1:351353/250‑1 (MQ=255)                                                                                                                                                       tATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGa                                                                                               >  2:180671/1‑250 (MQ=255)                                                                                                                                                        aTGCATCATGTCCCAGTTGTAAGAACGCCTTTCGACGACAATCAAGCAATCAGGGTTCACATCGGTAATAGAGAAACCGGCTTTGTACAGCTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGGCACAGGAAATAGGCAGTCGCCATCGCAGCGGTATCAAACACGCTGTCAGGTACATCGACACCTGCGGCGGCAAAGCGGTTCGCCAGATCtgacacagcctccgaa                                                                                              >  2:217766/1‑235 (MQ=255)                                                                                                                                                         tGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGACATCGGCAGTCGCCATCGCCAAGGTCTAAAACACGCTGTCAGGTAAATCGCCACCTGCGGTGGCAAAGCTGTTCGCCAGATCTTGCCCAGTCTGCGAAgg                                                                                            >  2:463960/1‑251 (MQ=255)                                                                                                                                                            atcatGTCCCAGTTGTAGGAACGAGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAACTCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGACGGAt                                                                                          >  2:63901/1‑250 (MQ=255)                                                                                                                                                               atGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCCGTCGCCATCGCAGAGGtcta                                                                                                                                                                    >  2:346321/1‑170 (MQ=255)                                                                                                                                                               atGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGtata                                                                                                                                                                    <  1:346321/173‑1 (MQ=255)                                                                                                                                                                       gtagtagtaACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGAACAGTTCATAAATCAGTGCGCCTTCGCCAACCACATACGCTTTCTTGCCTTCATGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAAGGACATCGTCCCCTGCGGTGGAAAAGAGGTTCGCCATATCTTGCCAAGTCTGCGCAGGAttataggacagc                                                                               >  2:169222/4‑239 (MQ=255)                                                                                                                                                                               aaCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGc                                                                      >  1:156528/1‑251 (MQ=255)                                                                                                                                                                                          ccAACAATCACGAAATCAGGGTTCACATCGTTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCttt                                                           <  1:88487/251‑1 (MQ=255)                                                                                                                                                                                                  cACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTTTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATa                                                    >  1:193422/1‑250 (MQ=255)                                                                                                                                                                                                                     tCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGc                                >  1:250722/1‑251 (MQ=255)                                                                                                                                                                                                                      cGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCt                               >  1:5988/1‑251 (MQ=255)                                                                                                                                                                                                                             aGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCAccc                         <  1:219229/250‑1 (MQ=255)                                                                                                                                                                                                                                 aaaCCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTAc                    <  1:118434/251‑1 (MQ=255)                                                                                                                                                                                                                                        cTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACg             >  1:31350/1‑251 (MQ=255)                                                                                                                                                                                                                                                aGTTCATAAATCAGTGCGCATTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGATGCACCCGGTACGGCGACGTTATCGTg     >  2:388737/1‑251 (MQ=255)                                                                                                                                                                                                                                                   tCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAACAATTCCGCTGCCCCCGGTACGGCGACGTTATCGTGCAt  >  2:336789/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGAACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCAT  >  NC_000913/699792‑700284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑