Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,057	G→T	L242I (CTT→ATT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,057	0	G	T	89.7%	137.0 / 11.7	58	L242I (CTT→ATT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base T (31/21);  total (35/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01

TCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCAC  >  NC_000913/64813‑65293                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             tCGGCGAAACGGCGGTCAATTTCGTCCATTCGAACCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTGTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGt                                                                                                                                                                                                                                         <  1:315894/250‑1 (MQ=255)  cGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCCCCCCCGCCGCAGCGGAATACGGTa                                                                                                                                                                                                                                        >  1:229418/1‑250 (MQ=255)      gAAACGGCGGTCAATTTCCTCCCTTCGTTCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATTGAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAACCGACCTTTAGCC‑TGGGCAAAAAAAACGCCGTTTTTAAAGCCGTGCTCGCGCCCCTCCCGCTCACTGTTATCGCGCCCCCGCCGCCGCGGAATACGGTAACGcc                                                                                                                                                                                                                                   >  2:58536/1‑250 (MQ=255)       aaaCGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTc                                                                                                                                                                                                                                  <  1:248861/251‑1 (MQ=255)           ggcggTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGc                                                                                                                                                                                                                               >  1:384918/1‑250 (MQ=255)                  aaTTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGttttt                                                                                                                                                                                                                       >  2:375269/1‑251 (MQ=255)                    tttCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATCCGGTAACGCTCGGCATGTTTTTgc                                                                                                                                                                                                                     >  1:167010/1‑251 (MQ=255)                     ttCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgc                                                                                                                                                                                                                     <  1:159008/250‑1 (MQ=255)                               cGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGCCCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                                                                                                                                                                            >  1:68758/1‑249 (MQ=255)                                      aCGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCCGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAAc                                                                                                                                                                                                   >  1:224245/1‑251 (MQ=255)                                       cGGGTTATCGCTAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGCAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTACCGCTCGGCCTGTTTTTgaagaat                                                                                                                                                                                                                >  2:436815/1‑231 (MQ=255)                                       cGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTCCCAGCGAGAATGAAGAGAAATTCCAGAACGCTGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCAt                                                                                                                                                                                                                <  1:436815/237‑1 (MQ=255)                                        agattATAGATAGATTTTCCTTCGCATAATAACTCCGGGGCGACGGTTTACAGAGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGGGCCTCGAGACCGTCGATAATTAGACGACCTTGAGAC‑TGGGCAACAAAGACGGCGTTTTTAAAGCCGTGCTCGCGCTACTCCAGCACACTGATATCGCGCACCAGCCGCAGCGGAATCCGGTAACGCTAGGCATGTTATTGCAGACTTCGCAGCTAGAACtc                                                                                                                                                                                                 >  2:276648/4‑250 (MQ=255)                                           ttATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGcac                                                                                                                                                                                              >  2:86426/1‑251 (MQ=255)                                             aTCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTGGCAGCATGCGCAGATCGAACGGcacc                                                                                                                                                                                             <  1:129183/250‑1 (MQ=255)                                                            ttCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCAAACTGCACCACGTTCCACCCgatg                                                                                                                                                                              >  2:31334/1‑250 (MQ=255)                                                             tCGCCTAATAACTCCTGAGCTACTGTTGCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATCCCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACTCCGTTTTTAAAGCCGTGCTCGCGCCACTCTAGCTCACTTTTAGCGCGCCCCAGCCGCAGCGGAATACGGTAATGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCgatga                                                                                                                                                                             <  1:373119/250‑1 (MQ=255)                                                               gCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcc                                                                                                                                                                          >  1:27931/1‑250 (MQ=255)                                                               gCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATca                                                                                                                                                                          <  1:246427/251‑1 (MQ=255)                                                                                         ccAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCa                                                                                                                                                 <  1:58536/250‑1 (MQ=255)                                                                                           aGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGc                                                                                                                                              >  2:140129/1‑251 (MQ=255)                                                                                             cGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTACCGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGCTGATCACATCAGGATCGTAGTTGGCAAACCAGGCGt                                                                                                                                            >  2:395723/1‑251 (MQ=255)                                                                                                        agaAATTCCAGAACGCGGATTTCAGCGCCGCGATACCGCCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTcc                                                                                                                                 <  1:433952/251‑1 (MQ=255)                                                                                                                    aCGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTGTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTgcggg                                                                                                                     <  1:113202/251‑1 (MQ=255)                                                                                                                               aGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGAc                                                                                                          <  1:140129/251‑1 (MQ=255)                                                                                                                                   ccTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAACGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGCCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGCCGTAt                                                                                                      >  1:132493/1‑251 (MQ=255)                                                                                                                                            cGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTca                                                                                             <  1:195725/251‑2 (MQ=255)                                                                                                                                               cGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACt                                                                                                                                                                                                  >  2:234706/1‑147 (MQ=255)                                                                                                                                               cGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACt                                                                                                                                                                                                  <  1:234706/147‑1 (MQ=255)                                                                                                                                                gATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTCTTTAAAGCCGTGCACGCGCCACTCCACCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCACCCGATGATCACCTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTCCCAGTAGCTGCGGGCGGCTGCCGACggcttcacgctcaaaat                                                                                         >  2:311905/1‑235 (MQ=255)                                                                                                                                                  tAATTAGCCGCCCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGCCGTATTCCAGTTCAAAATcg                                                                                       >  2:361300/1‑250 (MQ=255)                                                                                                                                                   aaTTAGCCGCCCTTTAGCC‑TGGGCAAAAAAGTCGCCGTTTTAAAACCCGTGCTCGCGCCACGCCAGCTCACTGTTAGCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATcg                                                                                       <  2:224245/250‑2 (MQ=255)                                                                                                                                                    aatagtctaCCTGTAGCCGTGGCCAAAAAAGCTGCGGTTTTTAA‑GCCGTGCCCGCGCCACCCCAGCTCTCTGTTAGCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGCTTTGGCAGCAGTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACAGCAGGATCGTATTTGGCAAACCAGGCGTTGATTTCTGCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAggtcacaatcggg                                                                                     <  2:426816/243‑13 (MQ=255)                                                                                                                                                           gcccTTTAGCC‑TGGGCAAAAAAGCCGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCGAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcggat                                                                              <  2:64654/249‑2 (MQ=255)                                                                                                                                                           gACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcgg                                                                                >  2:92475/1‑246 (MQ=255)                                                                                                                                                           gACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACGGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcggag                                                                              <  1:243382/251‑1 (MQ=255)                                                                                                                                                             ccTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcac                                                                                                                                                                         >  1:425008/1‑158 (MQ=255)                                                                                                                                                             ccTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGGGCTCGCGCCACGCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcac                                                                                                                                                                         <  2:425008/158‑1 (MQ=255)                                                                                                                                                                 aaGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGAGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAAAGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCAACACGATCCAACCGATGATCACATCAGGATCGTAGATGGCAAAACAGGAGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAATTCGAGCGCGGAGGCGTCg                                                                        >  1:455459/2‑251 (MQ=255)                                                                                                                                                                     c‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCCTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACAGATTCCAGTTCAAAATCGAGCGCGGAGGCGTAGCCAt                                                                    >  2:241127/1‑251 (MQ=255)                                                                                                                                                                       tGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTAGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACAAGGCGTTGAGTTTTTCCAGGAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATAGAGCGCGGAGGCGTCGCCAtt                                                                   >  1:57941/1‑251 (MQ=255)                                                                                                                                                                         ggCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtct                                                                 >  1:304728/1‑251 (MQ=255)                                                                                                                                                                          gCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCAAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATtctc                                                                <  1:31334/251‑1 (MQ=255)                                                                                                                                                                           cAAAAAAGACGCCGTTTTTAAAGCAGTGCTCGCGCCACTCAAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctct                                                               >  1:392228/1‑250 (MQ=255)                                                                                                                                                                                  gACGCCGTTTTTAAAGCAGTGCTCGCGCAACTCAAGCTGGCTATTATCGCGCCAAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTAGCAGATCGAACTGCACCACGTTCCAACAGATGATCACATCAGGATCGTAGTTGGCAAACAAGGCGTTGAGTTTTTCCAGCAATTGCGGGCGGCTGGCGACGTATTACAGTTCGAAATCAAGCGAGGAGGCGTCGCAATTCTCCGGcacc                                                         >  1:150908/1‑247 (MQ=255)                                                                                                                                                                                     gCCGTTTTTAAAGCCGTGCTCGCGCCAATCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGc                                                      >  1:359698/1‑250 (MQ=255)                                                                                                                                                                                           tttAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCATTAGCTGCGGGCGGCTGGCGACTTATTCCAGTTCGAAATCGAGCGAGGAGGCGTCGCCATTCTCTGGCCCCAGAATATaa                                                >  2:340101/1‑250 (MQ=255)                                                                                                                                                                                           tttAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATaa                                                >  1:294193/1‑250 (MQ=255)                                                                                                                                                                                            ttAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGCGTTTTTCCAGTAGCTGCGGGCGGCTGGCGTCGTATTCCAGTTCAAACTCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCGGCATATAAAc                                              >  2:386700/1‑251 (MQ=255)                                                                                                                                                                                             tAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAAc                                              <  1:371396/250‑1 (MQ=255)                                                                                                                                                                                                            gcCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGc                                >  1:67694/1‑249 (MQ=255)                                                                                                                                                                                                              cACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCCCCATTCTCTGGCCCCAGCATATAAACCATCCGCTGCCCGCAGcc                            >  2:237215/1‑251 (MQ=255)                                                                                                                                                                                                                 tCCAGCTCGCTATTATCGCGCCCAAGACGCCGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATCTAAACAATGCGCTGCCCGCAGCCTTc                         >  2:171719/1‑251 (MQ=255)                                                                                                                                                                                                                 tCCAGCTCACTGTTATCGCGCCCCAGCCGCCGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAACATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAACCGATGCGCTGCCCGCAGCCTTc                         >  2:366417/1‑251 (MQ=255)                                                                                                                                                                                                                 tCCAGCTCACTGTTATCGCGCCCCAACCGCCGCGGAATACGGTCACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATCTAAACGATGCGCTGCCCGCAGCCTTc                         >  1:362278/1‑251 (MQ=255)                                                                                                                                                                                                                 cccAGCTCCCTGTTATCGCGCCCCAGTCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCGATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTc                         <  1:126160/250‑1 (MQ=255)                                                                                                                                                                                                                           tgtctccgcgCCCCAGCCGCAGCGGAATACGGTAACGCCCGGCATGGGTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGAGCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGAt                <  2:386715/244‑1 (MQ=255)                                                                                                                                                                                                                                        cagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGAATTCGCAGATCGAACTGCACCCCGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCAc  <  2:384918/247‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             TCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCAC  >  NC_000913/64813‑65293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑