Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,850	G→A	noncoding (11/77 nt)	argQ ←	tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,850	0	G	A	90.3%	74.6 / ‑3.0	31	noncoding (11/77 nt)	argQ	tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (12/16);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGA  >  NC_000913/2817609‑2818072                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                tttAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGCAGGTTACGGAGAATATGATGCATCCGGGAGGATTCGAACCTCCGCCCGCTCGGTTCGAAGCCGCGTACTATATCCAGATGAACTACGGATg                                                                                                                                                                                                                        >  2:276472/1‑251 (MQ=255)   tAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGc                                                                                                                                                                                                                       <  1:164957/250‑1 (MQ=255)      aaaTGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCg                                                                                                                                                                                                                   >  1:136077/1‑251 (MQ=255)        aTGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCggg                                                                                                                                                                                                                 <  1:154720/251‑1 (MQ=255)          gagaTATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGGTAATACCGCTATCTCTTCGATAACTTAATGGCTGAAGGGTACGGCGAATATGGTGCATCCGGGAGGATTCGAAGCTCCAACCGCTCGGGTAGTAGTCGTGTACTCTATCCAGTTGCACTACGGATGCCTCGGGa                                                                                                                                                                                                                >  2:375381/1‑250 (MQ=255)                      aaGGAAATTTTTTGGAGAAGATGGTGTATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTAc                                                                                                                                                                                                   <  1:240790/251‑1 (MQ=255)                             ttttttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGGGCATCCGGGAGGATTCGAAACTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCCGCTGAACTACGGATGCATCGGGAAATCtttctttactcctgtt                                                                                                                                                                                            >  2:304134/1‑237 (MQ=18)                                 ttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGACCTCTATCCAGCAGAACTACGGCGGCATCGGGAAACCTTATTCTACT‑GCTGaaatgg                                                                                                                                                                                       >  1:380225/1‑246 (MQ=21)                                            ggTGAATCCGGGATGATGACTCACGGCCGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCGGGGGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATAGGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCGATCCAGCGGAACTACGGATGCATCGGGAAAACTTATTCGACT‑GCTGATATTGATACCGctact                                                                                                                                                                            <  2:210321/251‑1 (MQ=18)                                              tGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAATTTCTTCAACT‑CCTGATATTGCTACCGctaata                                                                                                                                                                           >  1:457156/1‑247 (MQ=18)                                               gCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTGGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGATTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTaaa                                                                                                                                                                         <  1:27878/251‑1 (MQ=255)                                                cATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTTAACTTCGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAg                                                                                                                                                                        <  1:206457/251‑1 (MQ=255)                                                 aTCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGc                                                                                                                                                                       <  1:281859/251‑1 (MQ=255)                                                                tCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTCCGGATGCATCGGGAAAACTTATTCTACT‑GCGGATATTGCTACCGCTACTAAAGCCATATCAATTAag                                                                                                                                                          >  1:319844/1‑249 (MQ=255)                                                                  aCTGACGTTCGCCCTTCGGGCAGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACATATTCTACG‑TCTGATATTGAAACGGctataaatgcagtgacaatttcgaaat                                                                                                                                                      >  2:319638/1‑227 (MQ=18)                                                                           cGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCACTACTAAAGCCATATCAATTAAGAGATGGTGCATcc                                                                                                                                             <  1:459528/251‑1 (MQ=255)                                                                            gCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCg                                                                                                                                            <  1:347719/251‑1 (MQ=255)                                                                                 tCGGGCCGTTGCTGGCGCAACGTTATACTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATGGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATGCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGATATGGTGCATCCgtga                                                                                                                                         <  2:60143/249‑4 (MQ=255)                                                                                                 gcAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGAATCCGGTAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACAACGGCTGCATCGGGAAACCTTATTCTTCT‑GATGATATTAATACCGATACTAAAGCACTATCAATTAATAAATTGCACATCCGGGAGGATTTCTCGCCtttcgc                                                                                                                       >  1:214715/1‑247 (MQ=17)                                                                                                   acagTTATGCTTCCTGGTGCCTGCGGTTGATGACGCTATCTCTGCAATACCTTCATTGGTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAGCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATGTTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGGTGGTGCATCCGGGAGGATTACTCGCCTGGCGGCt                                                                                                                     <  2:163586/248‑1 (MQ=17)                                                                                                              tcctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGCGTACTATTTCCAGCTGAACTACGGATGCATCGGGAAAATTTATTC‑ACTTGCTGTTATTGAACCCACTACTAGAGCCCACTAAATTAAGAGAAGTGGCACCCGGGAGGATAACGCGCCTTGcagtccccccttcggg                                                                                                          >  2:435556/1‑236 (MQ=9)                                                                                                              tcctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCg                                                                                                            <  1:164705/249‑1 (MQ=255)                                                                                                                  ggTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGATGCATCCGGGAGGATTACTCGCCTAGCGGCTCGCCCTTTGTGCCGt                                                                                                      >  1:362745/1‑251 (MQ=255)                                                                                                                      ccTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGCGGTTTACTCGCCTTGCGGCTCGCCCtt                                                                                                              >  2:266111/1‑239 (MQ=255)                                                                                                                      ccTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCtt                                                                                                              <  1:266111/239‑1 (MQ=255)                                                                                                                                      tgagctctTCTATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGAATCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCGAGCCAGCTGAACTACGGATGCAACGGGAAAACTTa                                                                                                                                                                                                         <  2:243278/129‑1 (MQ=14)                                                                                                                                      cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTa                                                                                                                                                                                                         >  1:243278/1‑133 (MQ=255)                                                                                                                                            ctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCt                                                                              <  1:276472/249‑1 (MQ=255)                                                                                                                                                                            aaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTTCTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATa                                            <  1:288655/251‑1 (MQ=255)                                                                                                                                                                                                   cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCgg                      <  1:11239/250‑1 (MQ=255)                                                                                                                                                                                                                       tCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGgga  <  1:161820/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                TTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACT‑GCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGA  >  NC_000913/2817609‑2818072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑