Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,232	C→T	P183P (CCG→CCA)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,232	0	C	T	93.6%	135.5 / ‑0.6	47	P183P (CCG→CCA)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (21/23);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 6.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCC  >  NC_000913/64989‑65466                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           gACGCCGTTTTTAAAGCAGTGCTCGCGCAACTCAAGCTGGCTATTATCGCGCCAAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTAGCAGATCGAACTGCACCACGTTCCAACAGATGATCACATCAGGATCGTAGTTGGCAAACAAGGCGTTGAGTTTTTCCAGCAATTGCGGGCGGCTGGCGACGTATTACAGTTCGAAATCAAGCGAGGAGGCGTCGCAATTCTCCGGcacc                                                                                                                                                                                                                                      >  1:150908/1‑247 (MQ=255)    gCCGTTTTTAAAGCCGTGCTCGCGCCAATCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGc                                                                                                                                                                                                                                   >  1:359698/1‑250 (MQ=255)          tttAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCATTAGCTGCGGGCGGCTGGCGACTTATTCCAGTTCGAAATCGAGCGAGGAGGCGTCGCCATTCTCTGGCCCCAGAATATaa                                                                                                                                                                                                                             >  2:340101/1‑250 (MQ=255)          tttAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATaa                                                                                                                                                                                                                             >  1:294193/1‑250 (MQ=255)           ttAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGCGTTTTTCCAGTAGCTGCGGGCGGCTGGCGTCGTATTCCAGTTCAAACTCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCGGCATATAAAc                                                                                                                                                                                                                           >  2:386700/1‑251 (MQ=255)            tAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAAc                                                                                                                                                                                                                           <  1:371396/250‑1 (MQ=255)                           gcCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGc                                                                                                                                                                                                             >  1:67694/1‑249 (MQ=255)                             cACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCCCCATTCTCTGGCCCCAGCATATAAACCATCCGCTGCCCGCAGcc                                                                                                                                                                                                         >  2:237215/1‑251 (MQ=255)                                tCCAGCTCGCTATTATCGCGCCCAAGACGCCGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATCTAAACAATGCGCTGCCCGCAGCCTTc                                                                                                                                                                                                      >  2:171719/1‑251 (MQ=255)                                tCCAGCTCACTGTTATCGCGCCCCAGCCGCCGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAACATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAACCGATGCGCTGCCCGCAGCCTTc                                                                                                                                                                                                      >  2:366417/1‑251 (MQ=255)                                tCCAGCTCACTGTTATCGCGCCCCAACCGCCGCGGAATACGGTCACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATCTAAACGATGCGCTGCCCGCAGCCTTc                                                                                                                                                                                                      >  1:362278/1‑251 (MQ=255)                                cccAGCTCCCTGTTATCGCGCCCCAGTCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCGATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTc                                                                                                                                                                                                      <  1:126160/250‑1 (MQ=255)                                          tgtctccgcgCCCCAGCCGCAGCGGAATACGGTAACGCCCGGCATGGGTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGAGCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGAt                                                                                                                                                                                             <  2:386715/244‑1 (MQ=255)                                                       cagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGAATTCGCAGATCGAACTGCACCCCGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCAc                                                                                                                                                                               <  2:384918/247‑1 (MQ=255)                                                                       cGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTc                                                                                                                                                               <  2:294193/251‑1 (MQ=255)                                                                         gTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGACGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCa                                                                                                                                                              >  1:345117/1‑250 (MQ=255)                                                                             cGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTCGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCCTATAAACGATGCGATGCCCGCAGCCTTCCAGGCCGATGCAGTACTGCTCACCGTGGCggcagggtttcacatt                                                                                                                                                         >  2:283422/1‑237 (MQ=255)                                                                               cTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCactcacc                                                                                                                                                        >  2:40164/1‑244 (MQ=255)                                                                                 cGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAACCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGCTGCAGTACAGCTCCCCGTGGCggggggggttcattttcat                                                                                                                                                      >  2:107258/1‑234 (MQ=255)                                                                                   gCATGTTGTTGCAGCATTCGCAGAACGAACTGCACCGCGATCCAACCGATTATCACGTCAGGAGCGTTGTTGGCAAACCCAGCGTTGAGTGTTTCCAGTAGCTGCGTGCGGCTGGCGACTTAGTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGTCCCAGCATAGAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGTTGGTGGTTTCAAGATCaata                                                                                                                                                     <  2:330391/249‑1 (MQ=255)                                                                                     aTGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGaaa                                                                                                                                                 <  1:449900/251‑1 (MQ=255)                                                                                           ttGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAATATcaa                                                                                                                                                       >  1:388899/1‑237 (MQ=255)                                                                                           ttGCAGCATCCGCATATCGAACTGCACCCCTTGCCAACCGAGGATCCCATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATcaa                                                                                                                                                       <  2:388899/239‑3 (MQ=255)                                                                                                gcaTTCGCAGATCGCACTGCACCACGTTCCAACCGATCATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGCTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCAGGGGGGTTCGATATCAATAAAAACCCACTTggac                                                                                                                                      >  1:149321/1‑248 (MQ=255)                                                                                                    tCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTTACTATCACTAGAAAcaccctttcgcggcg                                                                                                                                  >  2:423131/1‑237 (MQ=255)                                                                                                                      cacGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCg                                                                                                                <  1:297404/251‑1 (MQ=255)                                                                                                                       ccGTTCCAACCGATGATCCAATCAGGATCGTAGTTGGAAAACCAGGCGTTGAGTTTTTCCAGTAGCGGCGGGCGGCTTGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCGCACCGTGGCGGGTGGTTTCAATAGCAATATAAACCCACTTGAGCGGCGGACGATAGTCGTGATGCgg                                                                                                               <  2:242779/250‑1 (MQ=255)                                                                                                                            ccAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTc                                                                                                           <  1:86426/250‑1 (MQ=255)                                                                                                                                 cggtgatCACCTCAGGTTCGTAGTTGGCAAACCAGGGGTGGAGTGTTTCCAGTAGCTGCGGGCGGTGGGCGATGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCTTTCCAGTCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACgg                                                                                                     <  2:362278/248‑1 (MQ=255)                                                                                                                                         acacaaGGATCGTAGTTGGCAAACCAGGCGTTCAGTTTTTCCAGAAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAAc                                                                                             <  2:124193/246‑1 (MQ=255)                                                                                                                                               ggATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCCATATCAATAGAAACCCCCTTGAGCGGCGGACGGTAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAgg                                                                                       >  1:107414/1‑250 (MQ=255)                                                                                                                                                gATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAgcg                                                                                      <  1:395723/251‑3 (MQ=255)                                                                                                                                                    gTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCAt                                                                                  <  1:171719/251‑1 (MQ=255)                                                                                                                                                         gtgCAAACCAGGGGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATAGCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGc                                                                             <  2:229418/249‑1 (MQ=255)                                                                                                                                                            cAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGTCATTAACGATAGCGCCATTGTGCat                                                                           >  1:355551/1‑250 (MQ=255)                                                                                                                                                             aaaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCatat                                                                         <  1:81881/251‑1 (MQ=255)                                                                                                                                                             aaaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCatat                                                                         <  1:241127/251‑1 (MQ=255)                                                                                                                                                             aaaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGAGGCGGGTTCCGACGGGACTTAAAGATAACGCAATTGTGCatat                                                                         >  1:64069/1‑251 (MQ=255)                                                                                                                                                                  aGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGGAGCGGTTTCCGACGGGCATTAACGATATCGCCATTGTGCctattcccc                                                                    >  1:461315/1‑246 (MQ=255)                                                                                                                                                                                  cagtagcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAATAGAAAACCACTTGAGCGGCGGACGAAAGGCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCCTTGTGCCTATCACCCTCGAccccacccggtg                                                    >  1:4662/7‑242 (MQ=255)                                                                                                                                                                                  cagtagcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGGCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGTCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCggtg                                                    <  2:355551/245‑1 (MQ=255)                                                                                                                                                                                       gcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAAGCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCggtgaggtg                                               <  1:283422/250‑1 (MQ=255)                                                                                                                                                                                                                cattacaaaatcgcgcgcggtggCGTCGCCATTCTCTGGACCCAGCATATAAACGCTGCGCTGCCCGCAGCCTTCCAGGCCTATGCAGTCCAGCTCACCGTGGCGGGTGGTTGCAATAGCGATAGAAGCCCAGTTGAGCGGCGGACGATAGTCGGGATGCTGTTTCAGACGGGCATTAACGAGAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGtt                      <  2:132493/230‑1 (MQ=255)                                                                                                                                                                                                                   ttcaaaaTCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAATAGAAACCCACCTTAGCGGCGGACGATAGGCGGGATGCGGTTTTAGACGGGCATTAAAGCAAGCGCCATTTTGCCTATCAACCTCTACCCACACCGGGGGGGGGAATAACCGCTCCCTCAGATAACGTTCTg                   >  1:83784/5‑251 (MQ=255)                                                                                                                                                                                                                           cgaGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCgggcg            <  1:32868/248‑4 (MQ=255)                                                                                                                                                                                                                                gcggaggCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATc      <  1:92475/249‑1 (MQ=255)                                                                                                                                                                                                                                    aggCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGcc  <  1:366417/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           GACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCC  >  NC_000913/64989‑65466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑