Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,373	C→T	T103T (ACC→ACT)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,373	0	C	T	100.0%	162.0 / NA	56	T103T (ACC→ACT)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/20);  total (36/20)

GCCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGC  >  NC_000913/4396128‑4396606                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             gCCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGaaaa                                                                                                                                                                                                                                         <  1:185125/251‑1 (MQ=255)  ccGCGGCGCGCTCTGATATGCAGGGTTCTAACGGTAATCAACGTGCGCGGATATCTTTGAGTTTTAGTTGCGATCCTGAGTAGGCGGTTAGCCATCAAAGCAAATGCACCGGGGCGCTTGATAGCAAACAAACGGGCGTGATTCAGTGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCGCTGGAACGCCTAAAGAGTCACAAACGCTACGGTTGTTGGTCGATCGTACTGAAAAc                                                                                                                                                                                                                                        <  2:292215/251‑1 (MQ=255)       aCGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGTTGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTa                                                                                                                                                                                                                                    >  2:427395/1‑250 (MQ=255)        cGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTaaa                                                                                                                                                                                                                                  <  1:147138/251‑1 (MQ=255)              ctGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGa                                                                                                                                                                                                                             >  1:261629/1‑250 (MQ=255)                      cAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGa                                                                                                                                                                                                                     >  1:250388/1‑250 (MQ=255)                       aGGTTTCTAACGGGAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAg                                                                                                                                                                                                                   <  2:463916/251‑1 (MQ=255)                        ggattCTAACGGTAATCAACAGGCGCGGATCACGTTGCGTTTTATTGGCGCTCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGCCTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGACGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGGGGTCGATATTCCTGAAAACGGTAAAAACGAAGCGGAGAAgg                                                                                                                                                                                                                  >  2:54357/4‑250 (MQ=255)                                  ggTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACg                                                                                                                                                                                                                                       <  1:371139/220‑1 (MQ=255)                                  ggTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACg                                                                                                                                                                                                                                       >  2:371139/1‑220 (MQ=255)                                      aTCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGGGAAGCGGCAGAATGgcagg                                                                                                                                                                                                    >  1:38658/1‑250 (MQ=255)                                         aaCAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAAt                                                                                                                                                                                                 <  1:407451/251‑1 (MQ=255)                                         aaCAGGCGCGGATAAAGTGGAGTTTTATTGGCGATCCTGAGTATGCGTTTAGACATAAAAGAAAAAGCGCCGTGGCGCTCGATATCAAAAAAAAGGGCGTGATTCAGGGACTGCCGTTGTTGTGCAGAGGCAAAAAACTGGTGAAGGCGATTCGCGCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGt                                                                                                                                                                                                                       <  1:152862/229‑1 (MQ=255)                                                                       gCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GAt                                                                                                                                                                  >  1:153896/1‑251 (MQ=255)                                                                        cGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGCTTAACGCC‑gctg                                                                                                                                                                 >  2:83721/1‑248 (MQ=255)                                                                           tCCTGATTATGCGTTTAGCCAACAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAGGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAACCGGTAAAACCGAAGCGGGGAAGCGGCAGAATGGCAGCAATTCAACTGCCGTCTTTACGGTTAAAGcccggtggg                                                                                                                                                               >  2:250879/1‑244 (MQ=255)                                                                              tGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGCACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAAAGCC‑GATGTg‑ccgc                                                                                                                                                          >  1:55322/1‑251 (MQ=255)                                                                                          tAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGCAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCCCCCCCGcctcccc                                                                                                                                              >  2:107248/1‑249 (MQ=255)                                                                                          tAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGGGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCCCCGCcccctc                                                                                                                                              >  2:336133/1‑251 (MQ=255)                                                                                                 cAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCgccgcc                                                                                                                                       >  1:53893/1‑251 (MQ=255)                                                                                                  aaaGCAAACGCACCGTGGCGCTCGATATCTCACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGTTTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGTTCTTACTGAACACGGTAACACCGAAGCGGTGGAGCGGCGGACTGGCAGTAATTACACTGACGTCTTTCCGATTTAAGCC‑GTTGGT‑CCGCCAccccaccctccgaccccc                                                                                                                                      >  2:75757/1‑235 (MQ=255)                                                                                                   aaGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACCCTGCCGTCTTTACGATTAACGCC‑GAT‑TGTCCGCCCCCACCCCCTCCcccgccc‑g                                                                                                                                    >  2:356579/1‑251 (MQ=255)                                                                                                        aaCGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGGAAAACCGAAGCGGTGTAGCGGCAGGATGGCAGCAATTACACTGTCTACTTTACGATTAACGCC‑GATTTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGtt                                                                                                                               >  1:369119/1‑251 (MQ=255)                                                                                                         aCGCACCGTGGCGCTCGATATCACACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCCGA‑GTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGttt                                                                                                                              >  2:79373/1‑250 (MQ=255)                                                                                                         aCGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTg                                                                                                                              <  1:109530/251‑1 (MQ=255)                                                                                                         aCGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTg                                                                                                                              >  1:348704/1‑251 (MQ=255)                                                                                                                    cgcTCGATATCAAACAAACGGGCGTGATTGAGGTACTGCCGTTGTGTTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAACCGTTAAAACCGAAGCGGTGAAGCGGAAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGtt                                                                                                                   <  1:90343/251‑1 (MQ=255)                                                                                                                       tCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCCGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTACAACCGAAGCGGTGAAGCGGCAGAATTGCAGCAATTACCCTTTCGTCTTTACGCTTAACGCC‑GGTGTG‑CCGCCACCGCCTCCTCCGCACCCC‑GCGGTTGCGAACAGCGTTGa                                                                                                                 >  2:52488/1‑250 (MQ=255)                                                                                                                                 acaaacGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAAAGCCTGCgg                                                                                                      >  1:355177/1‑251 (MQ=255)                                                                                                                                            gggtttctggacgccagttgttgtgCAGCTGCTATCATCTGGTTACGGCGCTCTGCTCTGTCACGCCTATAGATGCACAAATGCGCCGGCTTGGGTTCGATCTTACTGAAAACGGTAAAACTGAAGCGTGGAAGCGTCAGAATGCCAGCACTTTCACTTCCGTCTTTTCGGTTAACGCA‑GGTGTG‑CCTCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACTCCTGAGGttcacgcactg                                                                                           <  2:155158/235‑10 (MQ=255)                                                                                                                                                 gaGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCCCAAACGCTACGGCTGGGGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGt                                                                                      <  1:173445/250‑1 (MQ=255)                                                                                                                                                    ggACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCCCCCCCC‑GTGGTTGCGAAACGCGTTGAAACGCCGGCGGttttgccaccccgcgccac                                                                                   >  1:354910/1‑234 (MQ=255)                                                                                                                                                    ggACTGACGTTGTTGTTCAGAGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTCAAGATGCACAAACGCTACGGATGGTGGTCGAACTCACTGAAAACGGTAAAAACGAAACGGTGGCTCGGCCAAACGGCAGCAATTACCCTATCGGCTTTCCGATTAACGCC‑GATATG‑CCGCCACCGCCTCCTCCGCCGCCCCGTG‑TTGCGCAACGCGTTGACACGCCTGCCATTGTCGCACcgcactccac                                                                                   >  2:282979/1‑244 (MQ=255)                                                                                                                                                           cGTTGTTGTTCAGCGGAAATAATCTGGTGAAGGGGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAATCGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCg                                                                            <  2:235286/251‑1 (MQ=255)                                                                                                                                                              tgttgttCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACACACGCTACGGATGGCGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGCCTTCACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGGTGTCGCACCCCGAGTCAGCGAACCGgcg                                                                         >  1:27097/1‑251 (MQ=255)                                                                                                                                                                     cAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGaa                                                                                                                                                                                                                    <  2:141438/108‑1 (MQ=255)                                                                                                                                                                     cAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGaa                                                                                                                                                                                                                    >  1:141438/1‑108 (MQ=255)                                                                                                                                                                      aGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATcc                                                                 >  1:241576/1‑251 (MQ=255)                                                                                                                                                                                atCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACg                                                       >  1:435199/1‑251 (MQ=255)                                                                                                                                                                                atCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACg                                                       >  1:389079/1‑251 (MQ=255)                                                                                                                                                                                  atGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGTTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGAAGAATGGCCGCAATTAAACCGTCGTCTTTACGATTAACGCC‑GATATG‑CCGCCAACGCCAACTCCGCCACCC‑GTGGGTGCGAAACACGGTGCAAAGCCTGCGGGTGTCGAACCGCGCGCCAGCGAACCGGCACGCacgacgtttaacaagg                                                      >  2:344304/2‑235 (MQ=255)                                                                                                                                                                                         aGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAAc                                              >  1:191494/1‑251 (MQ=255)                                                                                                                                                                                           gCGATTCGCTCTGGAACGCCTAAGGATGCACAAACGATACGGCTGGTGGTCGCTCTTACTGAAAACGGAAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGGATTTACGATTAACGCC‑GATGGG‑CCGCCACCGACTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTTTCGCACCGCGCGTCCACGACCCGGCGAGCAATCCGTTTACAACggtcagacaccg                                            >  2:207771/1‑241 (MQ=255)                                                                                                                                                                                             gATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGc                                           >  1:341072/1‑250 (MQ=255)                                                                                                                                                                                              aTTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCAc                                         <  1:83721/251‑1 (MQ=255)                                                                                                                                                                                                          aCGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGACTGGCAGCAATTACAATGTCGTCTTTACGATTAACGCC‑GATGAG‑CCGCCACCGCCCCCTCCGCCGCCC‑GTGGGTGCGAAACGCGTTGAAACTCCTGCGGGTGTCGCCCCCGGCGTCAGCGAACCGGCTCGACATCCGTTTTACACGGAAATGAAACACACTACGGGGGTTAt                             >  2:123933/1‑251 (MQ=255)                                                                                                                                                                                                          aCGCCCAAAGAGTCACAACCGCTACGGCTGGTGGTCAATCTTTCTGAAATCGTTAAAACCGAAGCAGTGAATCGGCAGAATGGCATCATTTACACTGTCTGCTTTACTATTCACTCC‑GATGTG‑CCGCCTCCGCCTCCTCCGCCGCCC‑GTGGTTGCGAATCGCGTTGATACTCCTGCTGTTGTCGCACCGCGCGTCAGCGAACCGGCGCTCAATCCGTTTAAAACGGAAAGTAACCGCCCTACTGGTGTTAt                             <  2:454030/251‑1 (MQ=255)                                                                                                                                                                                                                aaaGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATcagca                        <  1:336133/250‑1 (MQ=255)                                                                                                                                                                                                                   gATGCACAAACGCTACGGCTGGGGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACCCTGGCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTaa                    <  1:137542/251‑1 (MQ=255)                                                                                                                                                                                                                      gCACAAACCCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACAGAAGCGGTGAAGCGGAAGAATGTCAGCAATTACACTGTCGTCTTTCCGATTAACGCC‑GAGGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACTGGTGTTATCAGCAGTAATa                  <  2:369720/250‑1 (MQ=255)                                                                                                                                                                                                                       cacaAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACg                <  1:186168/251‑1 (MQ=255)                                                                                                                                                                                                                             cGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAAcg          >  1:236155/1‑251 (MQ=255)                                                                                                                                                                                                                              gCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTACAACGGAAAGGAACCGCACTACGGGTGTTATCAGCAGTAATACGGTCAcgc         >  2:171808/1‑251 (MQ=255)                                                                                                                                                                                                                              gCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAAcgc         >  1:235257/1‑251 (MQ=255)                                                                                                                                                                                                                                   ggCTGGTGGTCGTTCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCg    <  1:318864/251‑1 (MQ=255)                                                                                                                                                                                                                                      tggtggTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGc  >  1:425666/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             GCCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCC‑GATGTG‑CCGCCACCGCCTCCTCCGCCGCCC‑GTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGC  >  NC_000913/4396128‑4396606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑