Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,301,9920AT93.5% 80.1 / ‑3.0 32N271Y (AAT→TAT) oppAoligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (17/12);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

TTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAA  >  NC_000913/1301751‑1302235
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   
ttCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGc                                                                                                                                                                                                                                             >  1:68199/1‑250 (MQ=255)
      ggTGCCAAAATGCGCTATCGAGAAATTCGGCGAAAAACGGACCCAGCCTGGTAATATCGGCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTACCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGGATAACAGCATGCCGa                                                                                                                                                                                                                                      <  2:166707/251‑1 (MQ=255)
           cAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGaa                                                                                                                                                                                                                                 <  1:150071/251‑1 (MQ=255)
                      aTCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAAACAGGTAACCTATTTGACTATTGCTTCTGAAGTTACAGATGTCAACAGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGa                                                                                                                                                                                                                       >  2:27085/1‑250 (MQ=255)
                                  ggCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaaaaa                                                                                                                                                                                                             >  1:164337/1‑248 (MQ=255)
                                    cGAAAAATGGACCCAGCCTGGTAGTCTCGTCACAAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGAACTACTGGGACAACGCGAAAACCGTTATTAACCAGGGAACATATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGACTTGTGCCAGAGGTTGATAAAag                                                                                                                                                                                                           >  2:67732/1‑248 (MQ=255)
                                    cGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAag                                                                                                                                                                                                           >  1:344336/1‑248 (MQ=255)
                                     gAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAag                                                                                                                                                                                                           >  1:366720/1‑247 (MQ=255)
                                             gACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACg                                                                                                                                                                                               >  1:425228/1‑251 (MQ=255)
                                                     cTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCAc                                                                                                                                                                                       >  1:202668/1‑251 (MQ=255)
                                                     cTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCAc                                                                                                                                                                                       >  2:396399/1‑251 (MQ=255)
                                                                    ccAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtg                                                                                                                                                                        <  1:24088/251‑1 (MQ=255)
                                                                           gtccTATAACTTAAAAGATTGGGTCGTAAATGACCGAATCGTTCTTGATCGCAGCCCGACCTATTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCGTCTGAAGTTACCGATGTCAACCGCTACCTTAGGGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATTCCTACCTGTGCACtt                                                                                                                                                                 <  2:52285/249‑1 (MQ=255)
                                                                             ccTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTAc                                                                                                                                                               >  1:36043/1‑251 (MQ=255)
                                                                                ataCCTTAAAAGATTGGGTCGTAAACGAACGATTCGTTCTTGAACGCAGCACGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGAGGTGACATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCGGAAAAAAGAAATCCCGGACGACGTTCACGTTGATCCAAACCTGTGCACTTACTAt                                                                                                                                                            >  2:330430/1‑251 (MQ=255)
                                                                                ataCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCATCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTTAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTa                                                                                                                                                             <  1:222335/250‑1 (MQ=255)
                                                                                                 gTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACTAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCa                                                                                                                                           <  1:186648/251‑1 (MQ=255)
                                                                                                      aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGCCGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTTCTATTACGAAATTAACAACCAGAAAc                                                                                                                                      >  2:229946/1‑251 (MQ=255)
                                                                                                        acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCg                                                                                                                                    <  1:149137/251‑1 (MQ=255)
                                                                                                                            cGCAGACCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATGGCTTCTGAAGTTACTGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGCCTTATAACAGCATGCCTATCGAATGGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAATTTCACGTTGATCCATACCTTGGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgt                                                                                                                <  1:426974/251‑1 (MQ=255)
                                                                                                                                         attggaccaccgcgAAACCCGTTATAATCCAGTTCACCTATTTGCCGATTGCTGCGAAAGTTACCGATGTACACCGCTACCGGAGTGGTGAAATCGATAAGACGTATAACAGCATGCCGAGCGAATTTTTCCAGAAGCTGAAAAAAGAAATCCCGTACGAAGTCCACGTTGATCCATACCTGTGCACTTACTTTTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTg                                                                                                   <  2:378993/241‑1 (MQ=255)
                                                                                                                                              aacaacGCGAAAACCGTTATTAACCAGGTAACCTATTTACCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACt                                                                                              >  1:132580/1‑251 (MQ=255)
                                                                                                                                                      gAAAACAGTTCTTACCCAGGGAGCCTATTTGCATATTGATTCTGAAGTTACCGATGTCAACCGCTACAGTAGTGGTGAAATAGACATGACGAATAACAGCATGCCGAACGAATTGTTCCAGAAGCTGAAAAAAGAGATACCGGACGAAGTTCACGTTGAGCCATACGTGTGCACTTACTATTACGCAATTAACACCCAGAAACCGCAATACTACGATGTGCGTGTACGAACCGCACTGCACCTAGGTATgg                                                                                      >  2:178636/1‑251 (MQ=255)
                                                                                                                                                               tagtaACCAGTTATCCAATTTGCCATTTGCTCTTGAATTTACCGATTACAACCGCTACCGGAGGGGTGAAAACGACATGACGTATAAAAGCATGCCGATCGAATTGTTCCAGAAGATGAAAAAAGAGATCCCGGACGAAGTTCACGTTGACCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACGGAAACTAGGTATGGACCGCGat                                                                              <  2:366720/247‑1 (MQ=255)
                                                                                                                                                                            aCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGATAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaata                                                                 >  1:440928/1‑250 (MQ=255)
                                                                                                                                                                                     ccTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCa                                                                                                                                                                    >  2:395913/1‑142 (MQ=255)
                                                                                                                                                                                     ccTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCa                                                                                                                                                                    <  1:395913/142‑1 (MQ=255)
                                                                                                                                                                                      cTATTGCTTCTGAAGTTACAGATGTCAACAGCTACCGTAGTGGTGAACTCGACATGACGTATAACAGCATGCAGATAGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAGGTTCACGATGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCAATTCAACGATATGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCCTTGTTAATAAAGTGAAAgcg                                                      >  2:252606/1‑251 (MQ=255)
                                                                                                                                                                                          tGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAg                                                   <  1:337935/250‑1 (MQ=255)
                                                                                                                                                                                           gCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtg                                                                                                               >  2:140464/1‑189 (MQ=255)
                                                                                                                                                                                           gCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtg                                                                                                               <  1:140464/189‑1 (MQ=255)
                                                                                                                                                                                                                                           aTGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACAGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTTATGGCGCaa  >  2:435872/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   
TTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAA  >  NC_000913/1301751‑1302235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: