Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,794	T→C	A329A (GCA→GCG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,794	0	T	C	95.5%	61.3 / ‑3.6	22	A329A (GCA→GCG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (11/10);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

AAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTC  >  NC_000913/64547‑65021                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    aaaGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGGGTAAAAATAGCCTGATTTCCGTTTTATGGAAAATCTTCGTCACCAGCT‑CGCAATCTTTTCGGTTTTAAGTCGcc                                                                                                                                                                                                                                  >  2:45512/1‑251 (MQ=255)   aGCCCGGGCCGTGAATCCATCCCGTAGCCGCCAGGGCTGTCGTGTGGCGGCACTTCGCCGAGAGCAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAg                                                                                                                                                                                                                                <  2:174818/251‑1 (MQ=255)            cGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGttt                                                                                                                                                                                                                       <  2:451883/251‑1 (MQ=255)                            aGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGGGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCCATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAAcgg                                                                                                                                                                                                       >  1:242779/1‑251 (MQ=255)                              ccgccAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGGCTACCGGCAGGGCGTTCACCGTCGCACGTTCGAGTAAAAATTGCATGATTTTAGTTTTATGGGAAATATTCGTCCACAGCTACG‑AAACTTTCAGGTTATCAGTCGCCCGCGCAGGTTTTTATTTGGCGAAAcggcg                                                                                                                                                                                                     >  2:248861/1‑251 (MQ=255)                                                                                  cAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGAATGATTTCAGTTTTATGGCAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCACCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTAAATTTTGTCCAATCGGACCCCCGGGTTATCGAGAGATTTTTCTTTGccc                                                                                                                                                 >  1:421477/1‑250 (MQ=255)                                                                                                 gaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCg                                                                                                                                  <  2:276758/249‑1 (MQ=255)                                                                                                                                     cgccgTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTAGCGTAAAAATGGCATGATTTCAGTTTTGTGGAGGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGGCCATTCGGTCCCACGGGGTATCGATTGATTTTCCTTCGCCTAATAGCTCCTGCGGGACAGGTTCCAGCGAGAaagaaggataactccagaa                                                                                               >  2:43893/1‑237 (MQ=255)                                                                                                                                                     cGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGCCAATTTCGTCCCTTCGATCCCCCGGGTGTTCGATAAATTTTCCTTTGCCTAATAACTCCTGGGCGACGGGTTCCCGAGAGAAAGAAGAGAAAATCCAAAAcgagggttttagacccc                                                                              >  2:246427/1‑236 (MQ=255)                                                                                                                                                                    cgtcgctcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATaa                                                               <  1:41186/244‑1 (MQ=255)                                                                                                                                                                               cGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCG‑CGCAATCTTTCAGGGTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCt                                                    <  2:68618/251‑1 (MQ=255)                                                                                                                                                                                 aGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTTAGGTTATAAGGCGCCAGCGCATGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCttt                                                  <  1:268797/251‑1 (MQ=255)                                                                                                                                                                                     aaaaTGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAAATCCTGAGCGACGGTTTCCAGCGCGAATGAAGAGAAATTCCAGAACGCGGATTTAAGCGCCTCGCAACCGTCACTAATTAGCCGCCCTTTaacc                                              >  1:330391/1‑248 (MQ=255)                                                                                                                                                                                      aaaTGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTc                                                                                     <  1:187497/211‑1 (MQ=255)                                                                                                                                                                                        aTGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTgg                                           <  1:341097/251‑1 (MQ=255)                                                                                                                                                                                         tGGCATGGTTTCAGATTTATGGCAAATCTGCGTCACCAGCT‑CGCAATATTTCAGTTTATAAGTCGCCAGCGAAGGTTTATCTTCGGCGAAACGGCGGTCAATTTAGTCCCTTCGATCCCACGGGTTCTCGATCGATTTTCCTTTGCCTAATTACACCTGTGCGCCGGTTTCCAGGGAGACTGTAGATAAAATCCAGGAGGCGGATTTCAGCGCCACGATACCGTTCATGATTACACGACCTTTATCCtagg                                          >  2:373119/1‑248 (MQ=255)                                                                                                                                                                                          ggCATGATTTAAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAACCGGCGGTCACTTTCGTCCATTCGCTCCCACGGGGTATCGGAAGATTTTCCTTCGCCTAATAACTCCTGCGCGACGGTTTCCCGCGAGAATGAAGAGAAATTCCTGAACGCGGAGTTCATCGCATCGAGACCGTCGATAATTAACCGTCCtttcgcatgcgc                                         >  2:81881/1‑242 (MQ=255)                                                                                                                                                                                               gATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTAGATAATTAGCCGACCTTTAGCCTGGGCaaaa                                     <  1:45512/250‑1 (MQ=255)                                                                                                                                                                                                       ttttATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGATAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGTGCAAAAAAGACGcc                             <  1:137962/250‑1 (MQ=255)                                                                                                                                                                                                        tttatgGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGCCGGTTTCCAGCGCGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAACAAGACGCCGt                           >  1:124193/5‑251 (MQ=255)                                                                                                                                                                                                                       cGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGACACGGCGGGCAATTTACTCCACTCGATCCCACGGGTTATCGTAAGATTTTCCTTCGCCTCATAACTCCTGAGCGCCGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTTAGCGCCCCGTTACCGTCGATAATTCTCCGCCCTTTATCCTGGGCCGACAAGACGCCGTTTTTAAAGCCGTGcc            >  2:315894/1‑250 (MQ=255)                                                                                                                                                                                                                                 t‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCCTTCGATCCCACGGGGTATCGTTAGATTTTCCTTCGCCTAATAACTCCTGAGCGAAGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGCTTTCAGAGCCCCGATACCGTCGATAATTAACCGACCCTTAGCCTGGGCAAAAAAGACTCCGTTTTTAAAACCCTGCTCGCGCCAccc  >  2:32868/1‑249 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    AAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTC  >  NC_000913/64547‑65021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑