Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	76,364	G→A	A312A (GCC→GCT)	sgrR ←	DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	76,364	0	G	A	97.8%	137.7 / NA	46	A312A (GCC→GCT)	sgrR	DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/22);  minor base C (1/0);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGG  >  NC_000913/76119‑76610                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       tttATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGGTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTTGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCCCCCTGTGGACCTTTTAGCATCAGACCTCCagctg                                                                                                                                                                                                                                                    <  1:449290/250‑1 (MQ=255)              cATGGTGCCAACGGGGGATCAGTCCAGAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGAGTAGTGGGAGAAAGCACATAGCTTACCCAGTCCCTGACTGGCTGATTCGCCCCGCGAGGGGTGCGGCTGTCGAATATTAAATAGTATCAACCTTCCTCCAGGCGGCTTGCAATCTCTCTATCCTCACCCTGGGGACCTTGTAGCATCATACCTCCAGCTGTCTCGGCTGCAAtt                                                                                                                                                                                                                                      <  2:443901/251‑1 (MQ=255)                      cAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAg                                                                                                                                                                                                                              <  1:72597/251‑1 (MQ=255)                         cgtgggAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGCTTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAAc                                                                                                                                                                                                                           >  1:138917/4‑251 (MQ=255)                               aGCAGTCCATAAGCCGGGCACCACAGTTGCTGTGACTGTTCCTCAGCGAAATAGACCCGATTAAGTGGAGAATGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGAGCGGCTGTCGCATAGTAAATAGTAGCACCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCGTCACCCTGTTGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCAGCAATTTCCTGCAGTACCCAGAc                                                                                                                                                                                                                     >  2:15395/1‑251 (MQ=255)                                                                 ccgtctcatCAGCGAAATATACCACATTTGTTGGAGAAAGCTCATTGCTTTCCCAGGCCCTGACTTTATGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCCGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGTCCTTTTAGCATCAGACCTCCAGCTGGCTCTTCTGAAATTTCCGTCAGAACCCGCACGTTATCTGCGTCGATTAATGCCCGGTAACCgaa                                                                                                                                                                                    <  2:463043/242‑1 (MQ=255)                                                                    gTTCCTCAGCGAAATAGACAAGATTAGTTGGAGAAAGCACATAGCTTTCCCAGTCCCTGACTTGCAGATTCGCCCCGCGAGGGGTGCGGCTGTCGAAGAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTACAAGCTCTTTTTCCTCTCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAATTTCGACGATTAATGCCCGGTAACCgaagaa                                                                                                                                                                                 <  2:292930/250‑1 (MQ=255)                                                                          cAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCTATTAATGCCCGGTAACCGAAGAAGTCAt                                                                                                                                                                            <  1:334826/249‑1 (MQ=255)                                                                              gAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAAAGCCCGGTAACAGAAGAAGTCATCGAATg                                                                                                                                                                      >  2:83186/1‑251 (MQ=255)                                                                               aaaTAGACCAGATTAGTTGGAGAAAGCAAATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTTAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTAGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGAGTAATGCCCGGTAAGCAaagaagcaaacgaatgc                                                                                                                                                                     >  1:281737/1‑240 (MQ=255)                                                                                aaTAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGcc                                                                                                                                                                    <  1:199667/251‑1 (MQ=255)                                                                                 aTAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCCTCAGAACTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGCCGTTAACTTCGTCGGTTAATGCCCGGAAACCGAAGACGTCATCGAATGCCt                                                                                                                                                                   >  2:274075/1‑251 (MQ=255)                                                                                 aTAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTAGTCGGCACTTTCCGGCAGAACCCAGACGTTAACTTAGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCt                                                                                                                                                                   >  1:209247/1‑251 (MQ=255)                                                                                            tAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGt                                                                                                                                                        >  1:256690/1‑251 (MQ=255)                                                                                             aGTTGGAGAAAGCCCATAGCTTACCCAGTCCCTGACTTGCTGAATCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGGCATCGAATGCCTGAATTTTCAGt                                                                                                                                                        <  2:171929/250‑1 (MQ=255)                                                                                             aGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGtt                                                                                                                                                       >  1:433052/1‑251 (MQ=255)                                                                                                               gCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGACCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCgg                                                                                                                                      >  2:42548/1‑249 (MQ=255)                                                                                                                    cccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCAcc                                                                                                                                <  1:11980/251‑1 (MQ=255)                                                                                                                     ccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCg                                                                                                                               >  1:8375/1‑251 (MQ=255)                                                                                                                       agacccTGACTTGCTGATTCGCCCCGCGATGGTTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCACTCTCTTTTTCCTCACCCTGTGGACCTTTTAGCCTCAGACCTCCAGCTGGCTCGTCGGCCATTTCCGACAGAACCCAGACGTTAACTTCGTCGTTTAATGCCCGGTCACCGAAAAAGTCCTCAAATGCCTGCATTTTCCGTTGATTGGTGAGGTTGCggtacacagcc                                                                                                                             >  2:377362/4‑241 (MQ=255)                                                                                                                        gTCCCTGACTTGCTGATTCGTCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTGCAGTTGATTGGTGCTGTTGCGGATCACCGCAt                                                                                                                            <  2:246735/251‑1 (MQ=255)                                                                                                                        gTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCAt                                                                                                                            <  1:299426/251‑1 (MQ=255)                                                                                                                         tCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTa                                                                                                                                                                                                    <  2:355615/178‑1 (MQ=255)                                                                                                                         tCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTa                                                                                                                                                                                                    >  1:355615/1‑178 (MQ=255)                                                                                                                             tGACTTGCTGATTCGCCCCGCGATGGGTGAGGCTGACGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCCTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACggg                                                                                                                       >  1:339837/1‑250 (MQ=255)                                                                                                                              gACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGAc                                                                                                                      >  1:306916/1‑251 (MQ=255)                                                                                                                                       aTTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCg                                                                                                              >  1:455905/1‑250 (MQ=255)                                                                                                                                                                tCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGCTTGGTGCTGTTGCGGATCCCCGCATACGGACCGGTGCCGCTGGGATGGCTGGCAAAGTTACTg                                                                                      >  2:293906/1‑249 (MQ=255)                                                                                                                                                                 cGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCCCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCCTCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTAGCGGATCACCGACTACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGa                                                                                     >  2:312258/1‑249 (MQ=255)                                                                                                                                                                 cGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAg                                                                                    <  1:289332/250‑1 (MQ=255)                                                                                                                                                                         aaaTAGTAGCATCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCGCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTCCGTCGATTAATGCCCGGTAACCGAAGAAGTCATTGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTccc                                                                            <  1:33916/250‑1 (MQ=255)                                                                                                                                                                          aaTAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTGTCCGCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCGCGTCGGCAATTTCCGGCAGAACCCAGACGTTATCTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTCCCCAt                                                                          <  2:469820/251‑1 (MQ=255)                                                                                                                                                                                  cAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCgg                                                                  <  1:174369/251‑1 (MQ=255)                                                                                                                                                                                  cAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCGTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTGTCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAAGTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCgg                                                                  <  2:82413/251‑1 (MQ=255)                                                                                                                                                                                     ccTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAg                                                               <  1:320847/251‑1 (MQ=255)                                                                                                                                                                                              aGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGCTTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGAACGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGc                                                      >  1:345590/1‑251 (MQ=255)                                                                                                                                                                                                        cAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCa                                                                                                                                  <  1:417592/165‑1 (MQ=255)                                                                                                                                                                                                        cAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCa                                                                                                                                  >  2:417592/1‑165 (MQ=255)                                                                                                                                                                                                                    ccTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGCCCGGTGCCGATGGGATGGCTGGCAAAGTTACTGCGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAAc                                >  2:285955/1‑251 (MQ=255)                                                                                                                                                                                                                          ccTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAAc                          >  1:394285/1‑251 (MQ=255)                                                                                                                                                                                                                              tgGACCTTTTAGCATCAGACCTCCCGCTGGCTCGTCGGCAATTTCCGGCTGAAACCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCATGAATTTTCAGTTGCTTGGTGCTGATGCGGATCACCGCATACGGACCGGTGACGATGGGATGGCTGGCAAAGTTCCTGAGGGTTTCCCATTAGCGCGGCAGGATCATCGCCGGCACTTGCCCAAGCAATACAGGTAACCAGc                      >  2:279018/1‑251 (MQ=255)                                                                                                                                                                                                                                      ttAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCgg               <  1:42548/250‑1 (MQ=255)                                                                                                                                                                                                                                        agctgctgaccTCCAGCTGGCTCGTCGGCAATTTACGGCAGTACCCCGGCGTTAACTTCGGCGATTAATGCCTGGTAACCGATGAAGTTATCGAATGTCTGAATTTTCAGTTGATTTGTGCTGTTGCTGATCCCCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTTAGGTTTTCCCTTTCGCGCTGCAGGATCATCGCTGGAACTTGCCCCTGCAATACCGGTATCCAGCTGTCCGGtt             <  2:324942/241‑1 (MQ=255)                                                                                                                                                                                                                                        aGCATCAGCCCTCCAGCTGGCTCGTCGGCAAATTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGtt             <  2:8375/250‑1 (MQ=255)                                                                                                                                                                                                                                             cagaccTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTg        <  2:433052/246‑1 (MQ=255)                                                                                                                                                                                                                                                 ccTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGCATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGCTGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAAAGGTAACCAGCGGCCAGGTTGCGGGAGATg   >  2:294943/1‑251 (MQ=255)                                                                                                                                                                                                                                                  cTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTGAGATgg  >  1:151376/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       TTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGG  >  NC_000913/76119‑76610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑