Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,143,3770CT98.2% 163.7 / ‑4.5 57S283L (TCA→TTA) frwCputative PTS enzyme IIC component FrwC
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (25/31);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

ATGCTGATTTGCGTTGCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCC  >  NC_000913/4143139‑4143623
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      
aTGCTGATTTGCGTTGCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGCTTGGTCGTAAAAATTTCTCCGCCGAAGAGCGCGAAACCGGCAAAGCGGCACTGGGGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGc                                                                                                                                                                                                                                            >  1:25585/1‑251 (MQ=255)
       attGCGTTGCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGAAATGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCAGCTGCGTGTCCTTCCTTCCATCATGGTCGGTTTTTTTTGTGGTGCAGTag                                                                                                                                                                                                                                       >  2:101942/2‑248 (MQ=255)
               gCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCt                                                                                                                                                                                                                             >  1:381579/1‑251 (MQ=255)
               gCTCAGAGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTACTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGAGCAGTAACGGCGGAGCt                                                                                                                                                                                                                             >  2:152703/1‑251 (MQ=255)
                     ggTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCgg                                                                                                                                                                                                                       >  1:66965/1‑251 (MQ=255)
                              aCCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGGCGGAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCAATGGGGATGGGCTGTGTTGGCGTTACTGTAGGGGCGATTGCTTTCGCTGCTGCCGATCCGCTGCGCGTCATTCCTTCCCTCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTg                                                                                                                                                                                                              >  1:450984/1‑251 (MQ=255)
                              aCCGTGGTGGCCATCGCTGCCGTGGGAATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTg                                                                                                                                                                                                              >  1:148117/1‑251 (MQ=255)
                                cGTGGTGGCCATAGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAACATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGCAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGGGGTGCAGTAAATGAGGCGAGGGTCggggggcggtgct                                                                                                                                                                                                            >  2:266946/1‑240 (MQ=255)
                                         cATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGCTGGGGCTGGCGCCGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGCTTCCTTTCGCCGCTGCCGATCCGCTGCGTGGCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTACCGGCGGCGCTGGTCGGTGCGCGGTGCTCGGCAGGcg                                                                                                                                                                                                   >  2:432028/1‑250 (MQ=255)
                                          aTCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTg                                                                                                                                                                                                  <  1:243462/251‑1 (MQ=255)
                                              cTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTggggg                                                                                                                                                                                              >  1:362940/1‑250 (MQ=255)
                                                         aTTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATtgt                                                                                                                                                                                   <  1:95293/251‑1 (MQ=255)
                                                          tttGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATtgt                                                                                                                                                                                   <  2:410846/250‑1 (MQ=255)
                                                          tttGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGGCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATtgt                                                                                                                                                                                   <  1:128015/250‑1 (MQ=255)
                                                                  ccgccgCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCggt                                                                                                                                                                          <  1:201174/251‑1 (MQ=255)
                                                                       gcTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTg                                                                                                                                                                     <  1:303747/251‑1 (MQ=255)
                                                                             gagggggCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCa                                                                                                                                                               <  2:11216/248‑1 (MQ=255)
                                                                                    cTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTggg                                                                                                                                                        >  1:245534/1‑251 (MQ=255)
                                                                                      ggCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGtt                                                                                                                                                      >  1:370742/1‑251 (MQ=255)
                                                                                      ggCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGtt                                                                                                                                                      <  1:447214/251‑1 (MQ=255)
                                                                                      ggCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGGGATGGGCTGGGTGGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGtt                                                                                                                                                      <  1:268804/251‑1 (MQ=255)
                                                                                       gcgtctcggATTGGTCGTAAACATTTCGCCGCAGCATAGCGCGAAACCGGCAAAGCTGCACTTGTGATGGGTGGTGGTGGCGTTGCTGAAGGGGCTAGTCCTGGCGCCGCTGCCGATCCGCTGCGTGTCATTCCGTCCATCATTGTCGGGTTAGTGTGTGGTGCAGTAGCTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTATGATTGTGCTGCCGGTGTTTGAAGGCATGCTGGGtgg                                                                                                                                                     <  2:364122/243‑3 (MQ=255)
                                                                                                aTTGGTCGTAATAATTTCTCCGCAGAAGAGCGCGAAACCGGCACAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCAGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGGGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTTGATCgcagc                                                                                                                                            >  2:19298/1‑251 (MQ=255)
                                                                                                 ttGGTCGTAAAAATTTCTCCGCAGAATAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTTCTGAGGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCGGGGGTGGTCGGATTGtgc                                                                                                                                                                                 <  2:216929/213‑1 (MQ=255)
                                                                                                   ggTCGTAAAAATTTCTCCGCAGAAGAGCGCGATACCGGCAAAGCGGCACTGGTGCTGGGCTGTGTTGGCGTTACTGCAGGGGCGATTCCTTTCGCCGCTGCCGTTCCGGTGAGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTTCAGGACCTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTAGTGCCGGTGGTTGAAGGCACGCTgggctagttaccagcatg                                                                                                                                         >  2:153837/1‑236 (MQ=255)
                                                                                                   ggTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGt                                                                                                                                         <  1:242672/251‑1 (MQ=255)
                                                                                                         aaaaaTTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGTTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGTCTGt                                                                                                                                   >  1:50732/1‑251 (MQ=255)
                                                                                                          aaaaTTTCTCCGCAGAAGAGCGCGAAACCGGCCAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTc                                                                                                                                  <  1:373694/251‑1 (MQ=255)
                                                                                                          aaaaTTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTc                                                                                                                                  <  1:291488/251‑1 (MQ=255)
                                                                                                            aaTTTCTCCGCAGAAGAGCGCGAAAACGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTGTGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGtggg                                                                                                                                >  1:348359/1‑248 (MQ=255)
                                                                                                                 ctcCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGCTGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTACTGCCGGTGGTTGACGGCAAGCTGGGTTTTATCGAAGCAGTGGCTGGCGGAgg                                                                                                                             >  2:120795/1‑248 (MQ=255)
                                                                                                                        gaagaGCGCGAAACCGGCAAAGCGGCACTGGTGCTGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCTGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAggggggac                                                                                                                    >  1:268977/1‑244 (MQ=255)
                                                                                                                         aagaGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACg                                                                                                                   <  1:154421/251‑1 (MQ=255)
                                                                                                                          agagCGCGAACCCGGTCAAGCGGGACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGAGCCGCTGCGTGTCAGTCCTGCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCGGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACgg                                                                                                                  <  2:117146/251‑1 (MQ=255)
                                                                                                                          agagCGCGAAACCGACAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGAGATTCCTTTCGCCGCTGCCGATCCGCTGCGAGTCATTCCTACCATCCTGGTCGGGTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGATATGCAGGCTGGGGTGGGCTGATTGGTCTGACGGTGGTTGAATGCAAGCTGGGTTGTATCGCAGCAGTGGCTGTTGGAgacgggggaaagg                                                                                                                  >  2:306223/1‑239 (MQ=255)
                                                                                                                             gcgcGAATCCGGCATAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGACCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGTTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTg                                                                                                               <  1:333309/251‑1 (MQ=255)
                                                                                                                                                gCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGGCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGGTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCGGaa                                                                                            <  1:432028/251‑1 (MQ=255)
                                                                                                                                                    tggtggtggGATGTGGGGGCGTTACTGAAGGGGCGATTCGTTTCGCCGCTTCCTAGCCGCTGCGTGTCAGTCCTGCCAGCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCGGGTCGGTGCGGAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAg                                                                                         <  2:466897/250‑1 (MQ=255)
                                                                                                                                                      gTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGtt                                                                                                                                                                      <  2:148445/171‑1 (MQ=255)
                                                                                                                                                                  ggtggcgttgttggagtggCGATTCCTGTCGCCGTTGCCGGTCCGCGGCGTTTCATTCCTGCCATCATGGTCGGTTTAGTTTGTGGTGCAGTATCTGCGGCGCTGGTCGGGGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGGGTCTGGCGGAGCAGGGGTGACGGTTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTaaat                                                                           <  2:437033/233‑2 (MQ=255)
                                                                                                                                                                         ttACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGCTTGTGCTGCCGGGGGTTGAAGGCAAGCTGGGTTATATCGCAGCCGTGGCTTTCGGCGCAGTGGTGGCGGGTGTTTGTGGGAACGGGCTGGAAAGGCTGGCGCGTAAAAATGGGttt                                                                   >  2:330359/1‑249 (MQ=255)
                                                                                                                                                                               aaGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACt                                                             <  1:411649/251‑1 (MQ=255)
                                                                                                                                                                                 ggggCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTTGGGAGGTCTGATTGTGCTGCAGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGGGACGGCTGTTTGTGTGAACGTGCTGAAAAGACTGGGGCGTAAAAAAGGGACTTCGACtg                                                            >  1:33631/1‑250 (MQ=255)
                                                                                                                                                                                       aTTCCTTTCGCCGAAGCCGATAAGCTGCGTGTCATTACTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGGGATGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCCGTGGCTGTCGGAGGAGTGGTGACCGCTTTTTGTGTGAAAGGGCTGAAAAGCATGGCGCGGAAAAATGGGATTTCGACtgagtaga                                                      >  2:449855/1‑245 (MQ=255)
                                                                                                                                                                                              gcgccgcTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTGTGTGGTGCAGTAACTGCGGCGCTGTTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAgac                                              <  1:376969/250‑1 (MQ=255)
                                                                                                                                                                                                 ccgcGGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTGGTGGGGCAGGAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTGCGACTGATGAAAAAGAAgacgac                                           <  2:398535/251‑1 (MQ=255)
                                                                                                                                                                                                         gATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCtggattt                                   <  1:396478/251‑1 (MQ=255)
                                                                                                                                                                                                                 gCGTGTCATTCCTTCCGTCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTggatttggattt                             <  1:349472/249‑1 (MQ=255)
                                                                                                                                                                                                                       cATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGAGACGGCTGGTTGGGTGAACGGGCTGAAAAGTCCGGCGGGTAAAAATTGGGCTTCGTCTGGTGAAAAAGAAGACGACCTGGGTGTGGATTTTGTaatt                      >  2:196490/1‑250 (MQ=255)
                                                                                                                                                                                                                        aTTCCTTCCATCATGGTCGGTTTAGGTTGTGGGGCTGTAACTGCGGCGCTGGTCGGTGCGCAGGGCTATGCAGGCGGGGGTGGTCGGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCCGTGGCTGTCGGAGCAGTGGTGACGGCTGGTTGTGTGAACGTGCTTAAAAGTCTGGCTCGGAAAAATGGGTCTACGTCTGATGAAAAAGAAGACGACCTGGATTTGGAATTTGAaattat                    <  2:66965/251‑2 (MQ=255)
                                                                                                                                                                                                                        aTTCCTTCCAGCAGGGTCGGTTTAGTTTGGGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCGTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaa                    <  2:148117/251‑1 (MQ=255)
                                                                                                                                                                                                                         ttccttccATCATGGTCGGTTTAGTTTGTGGTGCAGTAACGGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCGGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGGCTGGCGCGTAAAAATGGTTCTACGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaa                    <  2:5872/250‑1 (MQ=255)
                                                                                                                                                                                                                                catcatGGGCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTTGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGa            <  2:108495/251‑1 (MQ=255)
                                                                                                                                                                                                                                     tGGTCGGTTTAGTTTGTGGGGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGACAAAGAAGACGACCTGGGTTTGGATTTTGAAATTAATTAATTAAGGaa        >  1:252100/1‑250 (MQ=255)
                                                                                                                                                                                                                                      ggTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGTCTGGGGTGTTCTGATTGTGCTGACGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGTCTGTTTGTGTGAACGTGCTGAAAAGTCGGGCGCGTAAAAATGGGTCTTCGATTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGatcg      <  1:294032/251‑4 (MQ=255)
                                                                                                                                                                                                                                         cGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGGCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGGCCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGc   >  1:145480/1‑251 (MQ=255)
                                                                                                                                                                                                                                          ggTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGGCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGcc  >  2:78179/1‑251 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      
ATGCTGATTTGCGTTGCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCC  >  NC_000913/4143139‑4143623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: