Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,031	A→G	N250N (AAT→AAC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,031	0	A	G	87.9%	153.3 / 9.4	59	N250N (AAT→AAC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (4/3);  new base G (33/18);  total (37/22)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

CGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTC  >  NC_000913/64800‑65270                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                cgcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAAAGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGccccagccgc                                                                                                                                                                                                                               >  1:386715/1‑243 (MQ=255)  gcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAg                                                                                                                                                                                                                             <  1:43893/251‑1 (MQ=255)        ttATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCCCACTGTTATCGCGCCCCAGCCGCAGCGGaat                                                                                                                                                                                                                       >  1:64654/1‑250 (MQ=255)              tCGGCGAAACGGCGGTCAATTTCGTCCATTCGAACCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTGTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGt                                                                                                                                                                                                                  <  1:315894/250‑1 (MQ=255)               cGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCCCCCCCGCCGCAGCGGAATACGGTa                                                                                                                                                                                                                 >  1:229418/1‑250 (MQ=255)                   gAAACGGCGGTCAATTTCCTCCCTTCGTTCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATTGAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAACCGACCTTTAGCC‑TGGGCAAAAAAAACGCCGTTTTTAAAGCCGTGCTCGCGCCCCTCCCGCTCACTGTTATCGCGCCCCCGCCGCCGCGGAATACGGTAACGcc                                                                                                                                                                                                            >  2:58536/1‑250 (MQ=255)                    aaaCGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTc                                                                                                                                                                                                           <  1:248861/251‑1 (MQ=255)                        ggcggTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGc                                                                                                                                                                                                        >  1:384918/1‑250 (MQ=255)                               aaTTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGttttt                                                                                                                                                                                                >  2:375269/1‑251 (MQ=255)                                 tttCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATCCGGTAACGCTCGGCATGTTTTTgc                                                                                                                                                                                              >  1:167010/1‑251 (MQ=255)                                  ttCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgc                                                                                                                                                                                              <  1:159008/250‑1 (MQ=255)                                            cGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGCCCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                                                                                                                                                     >  1:68758/1‑249 (MQ=255)                                                   aCGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCCGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAAc                                                                                                                                                                            >  1:224245/1‑251 (MQ=255)                                                    cGGGTTATCGCTAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGCAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTACCGCTCGGCCTGTTTTTgaagaat                                                                                                                                                                                         >  2:436815/1‑231 (MQ=255)                                                    cGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTCCCAGCGAGAATGAAGAGAAATTCCAGAACGCTGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCAt                                                                                                                                                                                         <  1:436815/237‑1 (MQ=255)                                                     agattATAGATAGATTTTCCTTCGCATAATAACTCCGGGGCGACGGTTTACAGAGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGGGCCTCGAGACCGTCGATAATTAGACGACCTTGAGAC‑TGGGCAACAAAGACGGCGTTTTTAAAGCCGTGCTCGCGCTACTCCAGCACACTGATATCGCGCACCAGCCGCAGCGGAATCCGGTAACGCTAGGCATGTTATTGCAGACTTCGCAGCTAGAACtc                                                                                                                                                                          >  2:276648/4‑250 (MQ=255)                                                        ttATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGcac                                                                                                                                                                       >  2:86426/1‑251 (MQ=255)                                                          aTCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTGGCAGCATGCGCAGATCGAACGGcacc                                                                                                                                                                      <  1:129183/250‑1 (MQ=255)                                                                         ttCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCAAACTGCACCACGTTCCACCCgatg                                                                                                                                                       >  2:31334/1‑250 (MQ=255)                                                                          tCGCCTAATAACTCCTGAGCTACTGTTGCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATCCCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACTCCGTTTTTAAAGCCGTGCTCGCGCCACTCTAGCTCACTTTTAGCGCGCCCCAGCCGCAGCGGAATACGGTAATGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCgatga                                                                                                                                                      <  1:373119/250‑1 (MQ=255)                                                                            gCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcc                                                                                                                                                   >  1:27931/1‑250 (MQ=255)                                                                            gCCTAATAACTCCTGAGCGACGGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATca                                                                                                                                                   <  1:246427/251‑1 (MQ=255)                                                                                                      ccAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCa                                                                                                                          <  1:58536/250‑1 (MQ=255)                                                                                                        aGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGc                                                                                                                       >  2:140129/1‑251 (MQ=255)                                                                                                          cGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTACCGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGCTGATCACATCAGGATCGTAGTTGGCAAACCAGGCGt                                                                                                                     >  2:395723/1‑251 (MQ=255)                                                                                                                     agaAATTCCAGAACGCGGATTTCAGCGCCGCGATACCGCCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTcc                                                                                                          <  1:433952/251‑1 (MQ=255)                                                                                                                                 aCGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTGTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTgcggg                                                                                              <  1:113202/251‑1 (MQ=255)                                                                                                                                            aGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGAc                                                                                   <  1:140129/251‑1 (MQ=255)                                                                                                                                                ccTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAACGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGCCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGCCGTAt                                                                               >  1:132493/1‑251 (MQ=255)                                                                                                                                                         cGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTca                                                                      <  1:195725/251‑2 (MQ=255)                                                                                                                                                            cGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACt                                                                                                                                                                           <  1:234706/147‑1 (MQ=255)                                                                                                                                                            cGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACt                                                                                                                                                                           >  2:234706/1‑147 (MQ=255)                                                                                                                                                             gATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTCTTTAAAGCCGTGCACGCGCCACTCCACCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCACCCGATGATCACCTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTCCCAGTAGCTGCGGGCGGCTGCCGACggcttcacgctcaaaat                                                                  >  2:311905/1‑235 (MQ=255)                                                                                                                                                               tAATTAGCCGCCCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGCCGTATTCCAGTTCAAAATcg                                                                >  2:361300/1‑250 (MQ=255)                                                                                                                                                                aaTTAGCCGCCCTTTAGCC‑TGGGCAAAAAAGTCGCCGTTTTAAAACCCGTGCTCGCGCCACGCCAGCTCACTGTTAGCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATcg                                                                <  2:224245/250‑2 (MQ=255)                                                                                                                                                                 aatagtctaCCTGTAGCCGTGGCCAAAAAAGCTGCGGTTTTTAA‑GCCGTGCCCGCGCCACCCCAGCTCTCTGTTAGCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGCTTTGGCAGCAGTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACAGCAGGATCGTATTTGGCAAACCAGGCGTTGATTTCTGCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAggtcacaatcggg                                                              <  2:426816/243‑13 (MQ=255)                                                                                                                                                                        gcccTTTAGCC‑TGGGCAAAAAAGCCGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCGAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcggat                                                       <  2:64654/249‑2 (MQ=255)                                                                                                                                                                        gACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcgg                                                         >  2:92475/1‑246 (MQ=255)                                                                                                                                                                        gACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACGGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcggag                                                       <  1:243382/251‑1 (MQ=255)                                                                                                                                                                          ccTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcac                                                                                                                                                  >  1:425008/1‑158 (MQ=255)                                                                                                                                                                          ccTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGGGCTCGCGCCACGCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATcac                                                                                                                                                  <  2:425008/158‑1 (MQ=255)                                                                                                                                                                              aaGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGAGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAAAGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCAACACGATCCAACCGATGATCACATCAGGATCGTAGATGGCAAAACAGGAGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAATTCGAGCGCGGAGGCGTCg                                                 >  1:455459/2‑251 (MQ=255)                                                                                                                                                                                  c‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCCTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACAGATTCCAGTTCAAAATCGAGCGCGGAGGCGTAGCCAt                                             >  2:241127/1‑251 (MQ=255)                                                                                                                                                                                    tGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTAGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACAAGGCGTTGAGTTTTTCCAGGAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATAGAGCGCGGAGGCGTCGCCAtt                                            >  1:57941/1‑251 (MQ=255)                                                                                                                                                                                      ggCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtct                                          >  1:304728/1‑251 (MQ=255)                                                                                                                                                                                       gCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCAAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATtctc                                         <  1:31334/251‑1 (MQ=255)                                                                                                                                                                                        cAAAAAAGACGCCGTTTTTAAAGCAGTGCTCGCGCCACTCAAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctct                                        >  1:392228/1‑250 (MQ=255)                                                                                                                                                                                               gACGCCGTTTTTAAAGCAGTGCTCGCGCAACTCAAGCTGGCTATTATCGCGCCAAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTAGCAGATCGAACTGCACCACGTTCCAACAGATGATCACATCAGGATCGTAGTTGGCAAACAAGGCGTTGAGTTTTTCCAGCAATTGCGGGCGGCTGGCGACGTATTACAGTTCGAAATCAAGCGAGGAGGCGTCGCAATTCTCCGGcacc                                  >  1:150908/1‑247 (MQ=255)                                                                                                                                                                                                  gCCGTTTTTAAAGCCGTGCTCGCGCCAATCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGc                               >  1:359698/1‑250 (MQ=255)                                                                                                                                                                                                        tttAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCATTAGCTGCGGGCGGCTGGCGACTTATTCCAGTTCGAAATCGAGCGAGGAGGCGTCGCCATTCTCTGGCCCCAGAATATaa                         >  2:340101/1‑250 (MQ=255)                                                                                                                                                                                                        tttAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATaa                         >  1:294193/1‑250 (MQ=255)                                                                                                                                                                                                         ttAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGCGTTTTTCCAGTAGCTGCGGGCGGCTGGCGTCGTATTCCAGTTCAAACTCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCGGCATATAAAc                       >  2:386700/1‑251 (MQ=255)                                                                                                                                                                                                          tAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAAc                       <  1:371396/250‑1 (MQ=255)                                                                                                                                                                                                                         gcCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGc         >  1:67694/1‑249 (MQ=255)                                                                                                                                                                                                                           cACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCCCCATTCTCTGGCCCCAGCATATAAACCATCCGCTGCCCGCAGcc     >  2:237215/1‑251 (MQ=255)                                                                                                                                                                                                                              tCCAGCTCGCTATTATCGCGCCCAAGACGCCGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATCTAAACAATGCGCTGCCCGCAGCCTTc  >  2:171719/1‑251 (MQ=255)                                                                                                                                                                                                                              tCCAGCTCACTGTTATCGCGCCCCAGCCGCCGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAACATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAACCGATGCGCTGCCCGCAGCCTTc  >  2:366417/1‑251 (MQ=255)                                                                                                                                                                                                                              tCCAGCTCACTGTTATCGCGCCCCAACCGCCGCGGAATACGGTCACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATCTAAACGATGCGCTGCCCGCAGCCTTc  >  1:362278/1‑251 (MQ=255)                                                                                                                                                                                                                              cccAGCTCCCTGTTATCGCGCCCCAGTCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCGATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTc  <  1:126160/250‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                CGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCC‑TGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTC  >  NC_000913/64800‑65270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑