Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 553,161 G→A intergenic (‑61/+57) purE ← / ← lpxH N(5)‑carboxyaminoimidazole ribonucleotide mutase/UDP‑2,3‑diacylglucosamine diphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913553,1610GA95.5% 116.6 / ‑3.2 44intergenic (‑61/+57)purE/lpxHN(5)‑carboxyaminoimidazole ribonucleotide mutase/UDP‑2,3‑diacylglucosamine diphosphatase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (23/19);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

CCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCT‑GATGTTTTTCCATCGCA  >  NC_000913/552916‑553398
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                
ccGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAag‑a                                                                                                                                                                                                                                            <  1:89512/251‑1 (MQ=255)
           ggCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGGCGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGGGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGaa                                                                                                                                                                                                                                  >  2:359735/1‑250 (MQ=255)
                     gCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGt                                                                                                                                                                                                                       >  1:424188/1‑251 (MQ=255)
                             gAACAGTTTAGCGGGGGTGGGGTGAGCAGAAACCACTTCAACGGGGTGCGGGACAGTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGGCGTAAAACTCCTGT‑GGGGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGa                                                                                                                                                                                                              <  2:120452/251‑1 (MQ=255)
                              aaCAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGaa                                                                                                                                                                                                             <  1:108146/251‑1 (MQ=255)
                                    gtattgggggTGCGGTGAGCAGAAACGCCGTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGTCGGCGAACTGCATGGTAGCCCAGTCGCGTTGGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGGCTTAAAACTCCTGT‑GGTGCACAACGCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑TGCTGTGAAATCAg                                                                                                                                                                                                        <  2:315724/245‑1 (MQ=255)
                                        cGGGGTTGCGGTGAGCAGAAACCACTTCAACGTTGTGCGGGACATGCATGAGTTCGAAGATTTCGGCGGCGAACTGCATGGTATCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAg                                                                                                                                                                                                   <  2:197120/251‑1 (MQ=255)
                                          ggggAGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGCTTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGCGGGCACAGAGAATAGCACGACAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGtt                                                                                                                                                                                                 >  1:431933/1‑251 (MQ=255)
                                               gCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCggg                                                                                                                                                                                            >  1:111311/1‑251 (MQ=255)
                                                           aacaaCTTCAACGTGGAGCGGGACATGCAGGATTTCGAAGATTTCGGAGGAGAACTGCATGGAAGCCCAGTCGCTTTTGGACCCCATCACGATGGAAACAAGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTACAGT‑GGAGCACAACTCTCGGCTTTCGAGGGCAAAGAGAAGAGCACGAAAAT‑AGAGCCAGGAAAAAGAATGAGT‑GGCTGAGAAAACAGCAACGATGCAGGTTTTTTAAAACg                                                                                                                                                                                >  2:262082/5‑251 (MQ=255)
                                                                     aCGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTCGCGGGTTTTTTAAAACGGAAAATGAAt                                                                                                                                                                      <  1:359735/251‑1 (MQ=255)
                                                                          gtgCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGACCTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAAATCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGATGTGAAATCAGAAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATc                                                                                                                                                                     >  1:59280/1‑247 (MQ=255)
                                                                                gACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGt                                                                                                                                                           <  1:74963/251‑1 (MQ=255)
                                                                                   aTTCAGGATTTCGAAGAATTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACCGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGGAACTGCAACAGCTCCACGtaat                                                                                                                                                        >  2:429201/1‑248 (MQ=255)
                                                                                      cAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATcc                                                                                                                                                      <  2:276563/250‑1 (MQ=255)
                                                                                                     ttCGGCGGCGAACTGCCTGGTAACCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGACAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTACAACGGAAAATGAATCAGCTAAACGTCATCCGCCGTGACTTTCAca                                                                                                                                       >  1:119959/1‑249 (MQ=255)
                                                                                                       cggcggCGAACTGCAGGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCAtt                                                                                                                                    <  1:461351/251‑1 (MQ=255)
                                                                                                            gcgAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAAcc                                                                                                                               >  1:100598/1‑251 (MQ=255)
                                                                                                              gAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCa                                                                                                                                                                                                         <  1:18999/175‑1 (MQ=255)
                                                                                                              gAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCa                                                                                                                                                                                                         >  2:18999/1‑175 (MQ=255)
                                                                                                                              cAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGAATGCCAGGCAcc                                                                                                             >  1:406346/1‑251 (MQ=255)
                                                                                                                                   gCTTTTGGACCCCATCACGATGGCGACACGCGCCGGGTTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGt                                                                                                         >  2:171172/1‑250 (MQ=255)
                                                                                                                                   gCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACAGGCGTCTTAAAACCCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTGGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTa                                                                                                        <  1:83747/251‑1 (MQ=255)
                                                                                                                                              ccATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGGGaaa                                                                                              >  2:96397/1‑250 (MQ=255)
                                                                                                                                              ccATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGG‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCTCGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGaaaa                                                                                             <  1:415771/251‑1 (MQ=255)
                                                                                                                                                aTCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGc                                                                                           >  1:374209/1‑251 (MQ=255)
                                                                                                                                                     gATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGATGTGAATTCAGCAAAGTTGCGGGTTTTTTACAACGGAAAATGAATCAGCTAAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCCGTACCCCGCGCAAAGAAgggt                                                                                      >  2:388922/1‑249 (MQ=255)
                                                                                                                                                      agggCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCCAAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGAAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTg                                                                                     <  1:77159/249‑1 (MQ=255)
                                                                                                                                                             cacGCGCCGGATTGTTAGGGGAAGACATGGTTGTTAAAGCTT‑TGTTGGTGCAAAACTTTCGCCTTTAGAGGGCACAGAGAATCGGA‑GGAAAGGAGAGCAAGGAAAGCGGTT‑CGTTGGCTGTGAAATCAGTAAAGTGGCGGGTTTTTTAAAACGTAAAATGAATAAGTTCAACTTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTg                                                                              <  1:438433/251‑1 (MQ=255)
                                                                                                                                                                    cGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATa                                                                        >  1:145120/1‑250 (MQ=255)
                                                                                                                                                                              ggggAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAAAAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCAc                                                             <  1:117330/250‑1 (MQ=255)
                                                                                                                                                                                       aTGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCg                                                    >  2:130550/1‑251 (MQ=255)
                                                                                                                                                                                        tGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCa                                                                                                                                                                    <  2:331403/138‑1 (MQ=255)
                                                                                                                                                                                        tGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCa                                                                                                                                                                    >  1:331403/1‑138 (MQ=255)
                                                                                                                                                                                                         gt‑ggggCACAACTCCCGGTTTTAGATGGCACAAAGAATAGCAGGAAAAG‑AGAGCAGGGAAAACGTTTGCGT‑GGTTGGGAAATCAGCAAAGTGGCGGTTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGAt                                  <  1:313682/251‑1 (MQ=255)
                                                                                                                                                                                                               gCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGAGGGGTATGCCCGAGGATAAGcc                             >  1:171590/1‑251 (MQ=255)
                                                                                                                                                                                                                cacaACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGAACCACGCGAAAAGCAGGATGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGAGAGGTATGCCCGTGGATCAGcc                             >  1:93888/1‑250 (MQ=255)
                                                                                                                                                                                                                 acaACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCAt                           >  1:225041/1‑251 (MQ=255)
                                                                                                                                                                                                                   aaCTCTCGGCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTg                         >  2:240075/1‑251 (MQ=255)
                                                                                                                                                                                                                         cGGCTTTAGAGGGCACAGAAAATAGCACGAAAAG‑AGAGCAAGGAAAACGGTTGCGG‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAACCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCT‑g                  <  1:191919/251‑1 (MQ=255)
                                                                                                                                                                                                                          ggCTTTAGAGGGCACAGAGAATAGCACGAAAAG‑AGAGCAAGGCAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGACAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCT‑g                  >  1:418528/1‑250 (MQ=255)
                                                                                                                                                                                                                                agagGACAAAGAGAATAGCACGAAAAG‑AGAGCAAGGAAATCGGTTGCGT‑GGCTGTGAAATCAGCAAATTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCACCGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTCCCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCT‑GATGtttt           <  1:171172/251‑1 (MQ=255)
                                                                                                                                                                                                                                    ggcccaacaaaTAGCACAAAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GTCTGGGAAATCAGCAAAGTTGCGGTTTTTTTAAACCGGAAAATGAACCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGTCTCCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATTCCCGTGGATCAGCCATTGCACCT‑GATGTTTTTCCa       <  1:311372/242‑1 (MQ=255)
                                                                                                                                                                                                                                         agagAATAGCACGAAATG‑AGAGCAAGGAAAACGGTTGCGA‑GGCTGTAAAATCAGCAAAGTTGCGGGATTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACATTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGCTATGTTCATGCCACGCCGGGCGATGGGTATGCCCGTGGATAACACATT‑CACCCCGATGTTTTTCCATCgac  >  2:467956/1‑249 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                
CCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGT‑GGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAG‑AGAGCAAGGAAAACGGTTGCGT‑GGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCT‑GATGTTTTTCCATCGCA  >  NC_000913/552916‑553398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑