Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,635	A→G	H382H (CAT→CAC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,635	0	A	G	100.0%	56.6 / NA	20	H382H (CAT→CAC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/10);  total (10/10)

ATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACC‑GAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCT  >  NC_000913/64389‑64878                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      aTGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGGGCTCTGGATCAGGATGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCa                                                                                                                                                                                                                                                   <  2:340605/251‑1 (MQ=255)     ttttCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCAtac                                                                                                                                                                                                                                                <  1:276107/250‑3 (MQ=255)              gagaACCAGGCATCGAGAAAACCTTCGGTACTGGGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAAGCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATa                                                                                                                                                                                                                                      <  2:218783/251‑1 (MQ=255)                  aCCAGGCATCGAGAAAACCTTGGGTACTGGGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGGGCGGATTATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAgaga                                                                                                                                                                                                                                  <  2:399455/251‑1 (MQ=255)                               aaaaCCTTCGGTACTGTGATCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACgccg                                                                                                                                                                                                                     >  1:198567/1‑251 (MQ=255)                                                       aacAGGCTGCGCCATGCCGTCCACCAGCCCGGCGGGATCAATCAGAAAGGGGCGGATGAACGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTcta                                                                                                                                                                                            <  2:171170/249‑3 (MQ=255)                                                            gCTGCGCAAGGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGAGGAGCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAAGCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTTGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGTTCTACCGGc                                                                                                                                                                                       <  2:429252/251‑1 (MQ=255)                                                                 gcCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCCGCACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGACAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGCTGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGcc                                                                                                                                                                                  >  1:59492/1‑251 (MQ=255)                                                                   cATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGCTGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGACGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTTGGCGCGACATAACCAGCGCGGTGCATCAGAGGAAAATAGAGATGACCCAACGCC‑CAACCCGATCCCCAGTGTCGGGCTACCGGCAGGCCgg                                                                                                                                                                                >  2:195725/1‑250 (MQ=255)                                                                           ccaccaGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCGTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtc                                                                                                                                                                        <  2:167099/251‑2 (MQ=255)                                                                                                  aGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGAGGTGAATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGGGGCGGGCTACCGGCAGGCCGGTCACCGTCGGCCGTTCGAGGAAAAAAGGCAt                                                                                                                                                 >  2:41186/1‑251 (MQ=255)                                                                                                                                  tttaaagtccggacccagtaccgAATCATAAAGCCCGGGCCGTGAATCCATCACGTGGCCGCCAGGGCTGGCGTGGGGCGGCAATTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGGTCGAGTAAAAATGGCATGATTTCAGTGTTATGGAAAATCTGCGTCAcc                                                                                                                  <  2:220103/228‑1 (MQ=255)                                                                                                                                                ccagtaccgAATCATAAAGCCCTGGCCGTGAATCCATCAAGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCCGCGCGGTGCATACGAGGAAAATAGCGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCAACCGGCAGGCCGTTCACCGTCGATCGTTAGAGTAAAAATGGCATGATTTCAGTTTTATGGGAAATCTGAGTCACCAGCT‑CGCAATCTTTc                                                                                                  >  1:324921/9‑251 (MQ=255)                                                                                                                                                               aaaGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGGGTAAAAATAGCCTGATTTCCGTTTTATGGAAAATCTTCGTCACCAGCT‑CGCAATCTTTTCGGTTTTAAGTCGcc                                                                                   >  2:45512/1‑251 (MQ=255)                                                                                                                                                                 aGCCCGGGCCGTGAATCCATCCCGTAGCCGCCAGGGCTGTCGTGTGGCGGCACTTCGCCGAGAGCAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAg                                                                                 <  2:174818/251‑1 (MQ=255)                                                                                                                                                                          cGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGttt                                                                        <  2:451883/251‑1 (MQ=255)                                                                                                                                                                            tGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCAACCGGCAGGCCGGTCACCGTCGGTCCTTCGGGTGAAAATGGCAGTATTTCAGTTTTATGGAAAATTTGCGTCACCATCC‑CGCTCACTTTCAGGCTATAAGtaccccacgccggtttat                                                                      >  2:371396/1‑234 (MQ=255)                                                                                                                                                                                          aGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGGGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCCATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAAcgg                                                        >  1:242779/1‑251 (MQ=255)                                                                                                                                                                                            ccgccAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGGCTACCGGCAGGGCGTTCACCGTCGCACGTTCGAGTAAAAATTGCATGATTTTAGTTTTATGGGAAATATTCGTCCACAGCTACG‑AAACTTTCAGGTTATCAGTCGCCCGCGCAGGTTTTTATTTGGCGAAAcggcg                                                      >  2:248861/1‑251 (MQ=255)                                                                                                                                                                                                                                                cAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGAATGATTTCAGTTTTATGGCAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCACCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTAAATTTTGTCCAATCGGACCCCCGGGTTATCGAGAGATTTTTCTTTGccc  >  1:421477/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACC‑GAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCT  >  NC_000913/64389‑64878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑