Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,800,435	G→A	G97D (GGT→GAT)	ygaM →	DUF883 domain‑containing protein YgaM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,800,435	0	G	A	92.6%	69.8 / NA	29	G97D (GGT→GAT)	ygaM	DUF883 domain‑containing protein YgaM
Reads supporting (aligned to +/- strand):  ref base G (2/0);  major base A (12/13);  minor base C (2/0);  total (16/13)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CAGGATATCCAGAATGATGTCAATCAATTAGCTGACAGCCTCGAATCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGAC  >  NC_000913/2800188‑2800669                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           cAGGATATCCAGAATGATGTCAATCAATTAGCTGACAGCCTCGAATCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGatat                                                                                                                                                                                                                                         <  1:447829/251‑1 (MQ=255)                                       ccTCGAATCCGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGt                                                                                                                                                                                                   <  2:242886/251‑1 (MQ=255)                                              tCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCCGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGAAGATATTTTTCTCGGCGCACTGTTAAGCATGCGCAAATCGTGGTGCaaaa                                                                                                                                                                                             >  2:420537/1‑250 (MQ=255)                                                        aGTCCTGGGGCAGCGACGCCAAAGGGGACGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCCGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGTGCGATGCCGTTGGCTGCGCGGCTTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGTCGCACGGTTAAGCATGCGCCAATCGTAGTGCAAAAATTAtaataa                                                                                                                                                                                   >  2:44461/1‑250 (MQ=255)                                                         gTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCCTTGCTGAAAGAAACCATGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGAGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTCGTGCATAAATGAtaataact                                                                                                                                                                                 >  1:70620/1‑249 (MQ=255)                                                              ggggCAACGACGCCCAAGGGGAAGCTGAAGCCGCAAGCAGTAAAGCTCATGCATTGCTGAAAGAAACCAGGGCGCGAATGCAGGGTCTTATTCGTGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCTCACTGTTAAGCATGCGCAAATCGTAGTTCAAAAATGATAATAAATAcgc                                                                                                                                                                             <  2:250712/250‑1 (MQ=255)                                                               tggCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGt                                                                                                                                                                           >  1:352260/2‑251 (MQ=255)                                                                   aGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCttt                                                                                                                                                                       >  1:146779/1‑251 (MQ=255)                                                                     cGACGCCAAAGGGGAAGCTTAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGa                                                                                                                                                                     <  2:376544/251‑1 (MQ=255)                                                                                   aaGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCACGGATTCTTTTGTTCGTGACAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTCACCCCGAAGCCTGTc                                                                                                                                                       >  1:32138/1‑251 (MQ=255)                                                                                    aGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGAGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGGTACGCATGCGCAAATCGTAGGGCAAAAATGATAATAAATAAGAGTCTTTGACCCCGAAGCCTgcct                                                                                                                                                      >  2:233960/1‑248 (MQ=255)                                                                                        gAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTc                                                                                                                                                    <  1:178437/249‑1 (MQ=255)                                                                                             cgcacgcaGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCgggggtt                                                                                                                                             >  1:72833/1‑248 (MQ=255)                                                                                              gccagtaGTAAAGCACAGTCATTACTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGTTTCCTGTAAGCCGCGCGCGATGTCGTTGGCTGCGCGTATTATTTTGTTCGTGAAAGACCCTTGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCCGGCGCAAATCGTAGTGCAAAAATGTTAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTc                                                                                                                                            <  2:349028/245‑1 (MQ=255)                                                                                                  gcaGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtttt                                                                                                                                        <  2:352260/251‑1 (MQ=255)                                                                                                             cAGGCATTGCTGAAAGAAACAAGGGCGCGAATGCATGGTAGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCATTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTATAGCGTGGGTACAGCAGCTGCGGAAGCTATTTTTATCGGAGCACGGATAAGCATTCGCAAATCGTAGAGCAAAAATACTAATAAATACGCGTCTTTGCCCCCGAAGCCTGTCATCGGGGTTTCTTTTTACCTGGtta                                                                                                                              >  2:373770/1‑248 (MQ=255)                                                                                                                gCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGAGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGactcc                                                                                                                          >  2:157797/1‑247 (MQ=255)                                                                                                                    tGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaa                                                                                                                       >  1:209927/1‑250 (MQ=255)                                                                                                                     gCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa                                                                                                                      <  1:384167/250‑1 (MQ=255)                                                                                                                               aCCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCATGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAc                                                                                                           >  1:98603/1‑251 (MQ=255)                                                                                                                                    ggCGCGAATGCATGTGCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGt                                                                                                      <  2:144689/251‑1 (MQ=255)                                                                                                                                         gAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAATGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGGCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGc                                                                                                 >  1:6945/1‑251 (MQ=255)                                                                                                                                                 ggTCTTACTCGCGTCCAGCAAGCCGCGCGCGATGTCGTTGGCTGCGCGGATTCTTTTGTGCGTGAAAGACCCTGGTGTAGCGTTGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTGCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGACACGCTCATATc                                                                                          <  2:374327/250‑1 (MQ=255)                                                                                                                                                       aCTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGCTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCCTATCCAGGGt                                                                                    >  1:430369/1‑250 (MQ=255)                                                                                                                                                          cgcgTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAAt                                                                                 <  1:125456/250‑1 (MQ=255)                                                                                                                                                                                              gcgGATTCTTTTGTTCGTGAAAGCACCTGGTGTAGCGTGGGTACAGCATCTGCGGTAGATATGTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGGCTTCGGTGTTTCTTTTTTCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATAGCCAGGGTAATTACGACCACTATTTGCTATATATTGGGTGTTTGAATc                                            <  2:456318/251‑1 (MQ=255)                                                                                                                                                                                                ggATTCTTTTGTTCGTGAAAGACCCTGGGGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATcgt                                          <  2:98270/251‑3 (MQ=255)                                                                                                                                                                                                          gTTCGTGAAAGACCCTGGAGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCACCCTACCCCGTCACGCTAATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACt                                 >  1:117280/1‑250 (MQ=255)                                                                                                                                                                                                           ttCGTGAAAGACCCTGATGTAGCGTGGGTACAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAACTAAGCGTCTTTGACCCCGATGCCTGTCTTCGGGGTTTATTTTTGCCTGGTGAATCACAAAAATCCCACTACCCCGTAACGCTCATATCCAGGGTAATTTCGACCACTATTTACTATATATTGTGTGGTGGAATCTTTTTTCAACTa                                >  2:405174/1‑250 (MQ=255)                                                                                                                                                                                                                                        aCAGCAGCTGCGGTAGATATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGGCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGAc  <  1:153597/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           CAGGATATCCAGAATGATGTCAATCAATTAGCTGACAGCCTCGAATCTGTATTGAAGTCCTGGGGCAGCGACGCCAAAGGGGAAGCTGAAGCCGCACGCAGTAAAGCTCAGGCATTGCTGAAAGAAACCAGGGCGCGAATGCATGGTCGTACTCGCGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTCTGTATCAACAGAGAGAC  >  NC_000913/2800188‑2800669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑