Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,594	A→C	F63V (TTT→GTT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,594	0	A	C	100.0%	61.4 / NA	23	F63V (TTT→GTT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (3/20);  total (3/20)

TCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGC  >  NC_000913/65347‑65836                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   ccggtaTGCGGTTTCAGAAGGGCATTAACGATAGCGCCTTTGGGCATATCACCCTCGACCCCCCCCGGTGAGGTGATAATCCGCTCCATCATATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCGTCACGCAGGCGCGTTTCGTAATTCATCAATTGGCGATGGTCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGcc                                                                                                                                                                                                                                                 <  2:359698/246‑1 (MQ=255)    gtagtcGGTTTCAGACGGGTATTAACGATAGCGCAATTGTGCAGATCCCCCCCGACCCACACCGGTGAGGTGATAAACTGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAAGTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCACCGGTGTCAGGCGAACGCCTTg                                                                                                                                                                                                                                              <  2:4662/246‑1 (MQ=255)             ttCAGACGGGCATTAACGATAGCGCCATTGTTCATACCACCCGCGACCCACCCCGGTGAGGTGAGAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCCGGCGCTGTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCGGGCGGTGAAAATCCGTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCt                                                                                                                                                                                                                                      <  2:67694/250‑1 (MQ=255)             ttCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTg                                                                                                                                                                                                                                     >  1:352382/1‑251 (MQ=255)                     ggCATTAATGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGt                                                                                                                                                                                                                                             <  1:149253/235‑1 (MQ=255)                             cGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAgcg                                                                                                                                                                                                                     <  2:45993/251‑1 (MQ=255)                              gATAGCGCCCTTGTGCTTATCACCCGCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAgcgc                                                                                                                                                                                                                    <  2:167010/251‑1 (MQ=255)                                            gCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATc                                                                                                                                                                                                       <  1:311905/250‑1 (MQ=255)                                               tatCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTTCGGCGAACGCCCTTTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATcggcg                                                                                                                                                                                                   >  1:72002/1‑251 (MQ=255)                                                    cccTCGCCCCACACCGGTGAGGTGCTAAACCGCTCCATCAGTTATCGTACTGGCGGGCGCACATCGGCCTCGGAGACGGTAACGCCACCTTCACTCAGGCGCTTATCGTAATTCAGCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCTGCGGGAAt                                                                                                                                                                                              <  2:86203/251‑1 (MQ=255)                                                                  cGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGAct                                                                                                                                                                                <  1:40164/251‑1 (MQ=255)                                                                     ggaggtgaTAAACCTCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCGCGGAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAAGTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATCCACCGGCGGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGTGGAACCTGATCGGCGGGAATAAACGCCACGGAGTCtt                                                                                                                                                                             <  2:304728/250‑1 (MQ=255)                                                                      gaggtgaTAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTg                                                                                                                                                                            <  1:237215/251‑1 (MQ=255)                                                                                      ccATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAc                                                                                                                                                             <  1:284149/250‑1 (MQ=255)                                                                                                                                         cACCTTCACGCAGGCGCTTTTCGTAATTCATCAATGGGCGAGGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGc                                                                                                         <  2:461315/251‑1 (MQ=255)                                                                                                                                                           tttCGTAATTCATCAATGGGGGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTAAAAATCCTTTAACGCCAGGGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCCCGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAgtg                                                                                        <  2:352382/250‑1 (MQ=255)                                                                                                                                                                                        cgACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGATAAACGCCACGGACTCTTGCGGGGCAAGCGGAAACTGCAACGGCCCGTTTTCCGGCGCCAGCCAGACGGAGACTTTTGTCCCTTTGGGGGGGTCCCGCCCGCGTCGGGGTAAAGTAAAAACTGCCCTCGCCAc                                                          >  1:36664/1‑251 (MQ=255)                                                                                                                                                                                                          cccGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCaaaa                                        <  1:239095/250‑1 (MQ=255)                                                                                                                                                                                                             ggCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCaaaaaaa                                     <  1:386700/251‑2 (MQ=255)                                                                                                                                                                                                                     gTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGACCCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTg                             <  1:340101/251‑1 (MQ=255)                                                                                                                                                                                                                                         ggggTGCCAGGCGAACGCCTTTTTCACCCTGCAAAAAAGGCTGAGCGCGGGGACCCTGATCGGGGGGAATAAACGCCACGGACCCTTGCGGGGCAAGCGTATCCTGCAACGGCCCGTTGCCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGttt         <  2:285959/249‑1 (MQ=255)                                                                                                                                                                                                                                            gtgtCAGGCGAACGCCTTGTTCACCCTGCAAAACTTGCTGAGCGCGGGGACCCTGATCGGCGGGAATAAACGCCACGTACTCTTGCGGTGCAAGCGTAACCGGCAACGGCCCGTTGTCCGCCGCCAGCCAGAAGGAGACTTCTGTCCTTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATCGCCTGGTTTCGTTTGAt      <  1:103446/251‑1 (MQ=255)                                                                                                                                                                                                                                                cAGGCGATCGCCTTGTTCCCCCGGCAAAATTTGCGGGGCGCGGGGAACCTGTACGGCGGTAATAACCGCCCCGGACTCTTGCGGTGCAAGCGTAACCGGCAGCGGCCCGTTGCCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCGCGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGc  <  2:221559/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   TCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGC  >  NC_000913/65347‑65836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑