Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,089	C→A	intergenic (+57/‑545)	cra → / → mraZ	DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,089	0	C	A	95.5%	118.5 / ‑2.7	44	intergenic (+57/‑545)	cra/mraZ	DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (15/27);  total (15/29)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

TTTGGCGATAACGAACTGCTCGACTTCTTACAGTGT‑CCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACATCAATATGCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGCCGATTTAACAAACACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCG  >  NC_000913/88844‑89334                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      tttGGCGATAACGAACTGCTCGACTTCTTACAGTGT‑CCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCCGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGCGTACGGGAATGGAc                                                                                                                                                                                                                                                  >  1:22565/1‑251 (MQ=255)     gCGAGAACTAACTGCTCGCATTCTGACAGTGT‑CCGGTGCTGGGAGGGGCTCAACGTCTCCGCGAGGGCGCAGAGCTTGTGCTGGAGATTGTCCCGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAAGCTCGATCGCCGCGGCGGGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGGGAATTCCCCGCGGGCGCGTAGAGTACGGGAATGGtcatca                                                                                                                                                                                                                                              <  2:168229/251‑1 (MQ=255)      cGATAACGAACTGCTCGACTTCTTACAGTGT‑CCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCa                                                                                                                                                                                                                                              >  1:46942/1‑250 (MQ=255)               aCTGCTCGACTTCTTACAGGGT‑CCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGTAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGGACGGGAATGGACATCAatagccttaa                                                                                                                                                                                                                                    <  2:450194/251‑8 (MQ=255)                  gCTCGACTTCTTACAGTGT‑CCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGTCATCAATATCCTTAAAGt                                                                                                                                                                                                                                 <  1:340214/251‑1 (MQ=255)                  gCTCGACTTCTTACAG‑GTGCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTaaa                                                                                                                                                                                                                                   <  2:120843/249‑1 (MQ=255)                   cgcGACTTCTTACAGGGT‑CCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACATCAATATGCTTAAAGTa                                                                                                                                                                                                                                <  2:193678/249‑1 (MQ=255)                       aCTTCTTACAGTGT‑CCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGAGTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGGAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATa                                                                                                                                                                                                                            <  2:19027/251‑1 (MQ=255)                             tactgtgt‑CCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGTACATCAATATCCTTAAAGTAAATAAGa                                                                                                                                                                                                                         <  2:133245/244‑1 (MQ=255)                                      cgggTGCTGGCAGTGGCTCAACGTCACAGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCGGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAAATAGACTATTCCTGAc                                                                                                                                                                                                              <  2:313047/249‑1 (MQ=255)                                          ggCTGGCAGTGGTTCAACTTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCGGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACtatt                                                                                                                                                                                                          <  2:96791/250‑1 (MQ=255)                                           gCTGGCAGTGGCTCAACGTCTCCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTTGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACtatt                                                                                                                                                                                                          <  1:315085/250‑1 (MQ=255)                                                  gTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATaa                                                                                                                                                                                                  <  1:261498/251‑1 (MQ=255)                                                                ccGCGATGTCGCAGAGCGTGTTCTGGAGATTGTCCTGGCAAACCTGGACGAACCGCGTAAGCCAAAACCTGTTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCTAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCTCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAAccc                                                                                                                                                                                    <  1:326744/251‑1 (MQ=255)                                                                 cgcgATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAAcccg                                                                                                                                                                                   <  1:201874/251‑1 (MQ=255)                                                                       gTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGt                                                                                                                                                                              <  1:234524/250‑1 (MQ=255)                                                                            agggcttgtgCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCTTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAAGACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGTACATAAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGttaatta                                                                                                                                                                        <  2:213883/245‑1 (MQ=255)                                                                                    tgCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCAcc                                                                                                                                                                 >  1:246101/1‑250 (MQ=255)                                                                                                  cTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATACGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTcaca                                                                                                                                                   >  2:2098/1‑250 (MQ=255)                                                                                                   tGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACaat                                                                                                                                                 >  1:85940/1‑251 (MQ=255)                                                                                                      caaaccTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTAAAACCCGCACGTTAATTAACACACCAGCTGAAATTTACaataat                                                                                                                                              >  2:194169/5‑251 (MQ=255)                                                                                                      cAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACaatact                                                                                                                                              >  1:283587/1‑249 (MQ=255)                                                                                                                     cgcgTAAGCCAAAACCTGGTTTAACGCGAATTAAACGTAATCTCTACCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACa                                                                                                                               <  2:196249/251‑1 (MQ=255)                                                                                                                         tAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAgcgc                                                                                                                           >  1:290522/1‑251 (MQ=255)                                                                                                                                                gcATTAAACGAAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAATAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGCCTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCCGCTGAAATTCACAATAATTAAGTGATATCGACAGCCAGTTTTTGCATTATTTGGTTACAt                                                                                                    >  1:59063/1‑251 (MQ=255)                                                                                                                                                  aTTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATCTCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTACGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATgc                                                                                                  >  1:420946/1‑251 (MQ=255)                                                                                                                                                     aaaCGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAACCGCGAACAAAACTACGAGCCAGGTGCATTTCCCTCTGGCGCGTAGAGGACGTGAATGGACATCAATATCCTTAAAGAAAATAAACCTATTCCTGACTATTATTGATATGTTCTTTTAAACCAGCCCGGGAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATAGCGCCCTCGCGTTTTTGCATTATTTTGTAACagtc                                                                                                  >  2:35983/1‑245 (MQ=255)                                                                                                                                                           aaTCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGACGCGAAAAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGa                                                                                          <  2:430056/250‑1 (MQ=255)                                                                                                                                                             ccgctATCGCCGCGGCGTGTTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAATTCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCCTTATTTTGTTACATGCAGAGATGAAtt                                                                                       <  2:238568/248‑1 (MQ=255)                                                                                                                                                                  aTCGCCGCGGCGTGCTTAGCCGTATCTAAGCCGTGAACAAAAATACGCGTCAGGTTAATGTTCCTCTGGCGCGTAGAGTACGTGAATGGACTGCAATATCCGTAAATTAAATAAGACTATTCCTGACTATTATTGAGAAGTTCTATTAAACCCGCCCGTTAAGTAACTCATCAGCTGAAATTCACAATAATTAATGGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGcc                                                                                    <  2:336737/249‑1 (MQ=255)                                                                                                                                                                     gccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGCACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCCTTATTTTGTTACATGCAGAGATGAATTGCCGAttt                                                                               >  1:282891/1‑251 (MQ=255)                                                                                                                                                                                  cAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACttt                                                                   <  1:309885/250‑1 (MQ=255)                                                                                                                                                                                       gTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTCTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTg                                                             <  1:363889/251‑1 (MQ=255)                                                                                                                                                                                         aGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACATCAATATGCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGTCGATTTAACAAACACTTTTCTTTGCt                                                           <  2:3501/251‑1 (MQ=255)                                                                                                                                                                                          gCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCtt                                                          >  2:159098/1‑251 (MQ=255)                                                                                                                                                                                           cTAAGCCGCGCACACAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGAACTTTTCTTTGCttt                                                         >  2:36265/1‑251 (MQ=255)                                                                                                                                                                                                      aCAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAAccc                                              <  2:214742/251‑1 (MQ=255)                                                                                                                                                                                                           aaTACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTgg                                         <  2:290522/251‑1 (MQ=255)                                                                                                                                                                                                              aCGCGCCATGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTGAAAGTAAATAATACTATTCCTGACGATTATTGATAAGTTCGTTGAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATGAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCATAGATGAAGTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCAt                                      <  2:282891/251‑1 (MQ=255)                                                                                                                                                                                                                             ttCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAg                        <  2:150198/250‑1 (MQ=255)                                                                                                                                                                                                                                       ggcgTAGAGTACGTGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACaggg             <  2:354659/250‑4 (MQ=255)                                                                                                                                                                                                                                             gagTACGGGAATGGAAATCAATATCCTCAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGtt       <  2:326671/251‑1 (MQ=255)                                                                                                                                                                                                                                             gagTACAGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAAGTAACTCACCAGCTGAAATTCTCAATAATTAAGTGATAGCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAAGTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGtt       <  2:393297/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCAACAGCTGAAATTCCCAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGCGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCg  >  2:402020/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      TTTGGCGATAACGAACTGCTCGACTTCTTACAGTGT‑CCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACATCAATATGCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGCCGATTTAACAAACACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCG  >  NC_000913/88844‑89334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑