Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 66,245 G→A T102T (ACC→ACT)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091366,2450GA97.3% 109.4 / ‑6.4 37T102T (ACC→ACT) araDL‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (17/19);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACC  >  NC_000913/65998‑66488
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    
gCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGGGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGCCTGGCCCGCCTGCGCCGAGAtagtgg                                                                                                                                                                                                                                                  >  2:413474/1‑251 (MQ=255)
         ccttcgtcattttGGCCCATGCAAATGGGCCGTGAGAATTGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTTCCGTTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGgcgc                                                                                                                                                                                                                                          <  1:272575/242‑1 (MQ=255)
           tCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGTCCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCCCTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGTAATCGACTGGCCCGCCTGCGCCCAGATAGtggcgtggcg                                                                                                                                                                                                                                           <  1:364440/247‑1 (MQ=255)
            cGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATCGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAg                                                                                                                                                                                                                                       <  1:206653/250‑1 (MQ=255)
                aTTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCCGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAgtggcgtggagggagggggt                                                                                                                                                                                                                                  >  1:443435/1‑240 (MQ=255)
                       gcccATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCTTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAAGGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTGGCTGGAATCGTCTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGca                                                                                                                                                                                                                             <  2:334298/249‑1 (MQ=255)
                            tGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCAGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTgtgggtgcgcaatgc                                                                                                                                                                                                                       >  2:155069/1‑237 (MQ=255)
                              aaaatgggCCGGGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATTCCATGTTTTGCGACGGTTTCTACGATGACGTTACCGGTTTCCCACTCAAATTCTCCTGTGATTTCTGCGTCGGTCATTTCGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGTTTGCTGGAACCGACGGGTCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATgccg                                                                                                                                                                                                                     <  1:71902/246‑1 (MQ=255)
                                      ccGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGGGTGTGTGCACAATGCCGCCCATgg                                                                                                                                                                                                              >  1:260598/1‑250 (MQ=255)
                                            gAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATGCCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCCCAATGCCGCCAATGGACGGGa                                                                                                                                                                                                        >  1:418595/1‑250 (MQ=255)
                                                ggACCAGGACGCCGGGCATTTGCGCTTCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTGCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGcc                                                                                                                                                                                                   <  1:335307/251‑1 (MQ=255)
                                                           ccGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAAAGCCGCCAATGGACGGGAATGCCTGATAGagc                                                                                                                                                                                          >  1:193923/1‑249 (MQ=255)
                                                                        cTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGGTGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGCCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGCATCGACTGGCCCGCCTGCGCCCAGATGGTGGCGAGGCGGGAGTGTGTGTGCACAATGCCGCCAATGTACGGGCATTCCTGATAGAGCAGTCGGTGAGTTggg                                                                                                                                                                           >  1:259780/1‑250 (MQ=255)
                                                                            aTCGATTCCCTGTTTTTCGAAGGTTTCTACGATGACTTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCgtat                                                                                                                                                                       <  1:128284/251‑3 (MQ=255)
                                                                            aTCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGTTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGGGCACAATGCCCCCAATGGACGGGAATGCCTGATAGAGGCGGCGGTGAGTTggggtat                                                                                                                                                                       >  2:198964/1‑246 (MQ=255)
                                                                               gATACCCTTTTTGTCGAAGTTTTCTACGATGCCTGTACCGTTTTCACACTCAGCTGCACCGTTGATTGCTGCGTCGGTTATTGTGCGGGTGCAGGGAATGGTGCCGTAGAAAGAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGGGGCGTGGCGCGAGTGTGTGGGCACAATGCCGTCAATGGACTGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCgt                                                                                                                                                                    <  2:36664/251‑2 (MQ=255)
                                                                                        tttttCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGGGGCGGGCt                                                                                                                                                           >  1:429037/1‑251 (MQ=255)
                                                                                               aaGGTTTCTTCGATGACGTTTCCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGTTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCT‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCgc                                                                                                                                                    <  1:324286/251‑2 (MQ=255)
                                                                                                        aCGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAAc                                                                                                                                           >  1:385792/1‑251 (MQ=255)
                                                                                                                                       caccGTTGATTGCTGCGTCGTTCATTTGGCGGGGGCAGGGAATGGTTCCGTAGAAATAGTCGGCGGTGGG‑GGTGCCGGTTGCTGGAATCGCCTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGCCGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGAc                                                                                                            <  2:170752/248‑1 (MQ=255)
                                                                                                                                        accGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGGGCACAATGCCGCCAATGGACGGGAATGCCTGGTAGAGCAGTCGGTGAGGTGGCGTATCGGGGGAGGGCTTTTTAGCACCTTCAACCCCTT‑CACCGGTTTCGGTGCTAaccaccaccc                                                                                                          >  2:90375/2‑250 (MQ=255)
                                                                                                                                        accGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGGGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATCGAGCAGGCGGTGAGTTGGCGGATCGGGGGGGGGCTTTTTCGCACCTTCAACCACTT‑CCCCGGTTTCGATGCTAACCACGAcc                                                                                                           >  2:420630/2‑250 (MQ=255)
                                                                                                                                         ccGTTGGTTTCTGCGTCAGTCATTTTGCGGGTGCAGGGGATGATGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGTCCGCCTGCGCCCAGATAGAGGCGTGGCGCGCGGGTGTGTGGACCATGCCGCCAATGGACGGGAATGCCTGATAGAGGAGTCGGTGAGGTGGCCTATCGGAGGAGGGGTTTTTCGCACCTTCAACCACTTTCACGGGTTT‑GATGcgaaacccggccc                                                                                                          >  2:80916/1‑238 (MQ=255)
                                                                                                                                         ccGTTGATTTCTGGGTCGGTCATTTTGCGGGGGCAGGGAATGGTGCCTTAGAAAGAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAAGCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCat                                                                                                         <  1:289008/251‑1 (MQ=255)
                                                                                                                                              gATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAggg                                                                                                                                                             >  1:443060/1‑195 (MQ=255)
                                                                                                                                                              aTTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGGATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGGGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGCTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCt                                                                                    >  1:157203/1‑251 (MQ=255)
                                                                                                                                                                          cAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGcc                                                                        <  1:124492/251‑1 (MQ=255)
                                                                                                                                                                                     gCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGa                                                             <  1:413474/251‑1 (MQ=255)
                                                                                                                                                                                     gCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGa                                                             <  1:198964/251‑1 (MQ=255)
                                                                                                                                                                                           gAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATTGACGCCGGAAGGTTTGATcaca                                                        >  1:133066/1‑250 (MQ=255)
                                                                                                                                                                                            aaaTAGTCGGCT‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACaaa                                                      <  1:379824/251‑1 (MQ=255)
                                                                                                                                                                                                       g‑tgggtggTGCCGGTTGTTGGATGCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGTCGCGAGGGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCCCCTTCAACCACTT‑CACCGGTTTCTATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCt                                           <  1:423751/251‑1 (MQ=255)
                                                                                                                                                                                                                tgCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGAAGCCGCGCTCGCGATc                                    >  1:293713/1‑250 (MQ=255)
                                                                                                                                                                                                                           gaaaccgcttggcccGCTTGCGCCCGGATAGTGGCGTGGCGTGAGTGTGTGTGCACAATGCCGCCAATGGACGGTAATGCCTGATAGAGCAGTCGGTGAGGTGGCGTATCGGAGGAGGGCTTTTTCGCAGTTTCAACCACTT‑CACCGTGTTCGATGCTAACCACGTCCATAGCGTCATCGCTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGAc                        <  1:427298/239‑1 (MQ=255)
                                                                                                                                                                                                                                   ctggcccGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGcccc                >  2:160563/5‑251 (MQ=255)
                                                                                                                                                                                                                                          gCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAg         <  1:41979/251‑1 (MQ=255)
                                                                                                                                                                                                                                               cgcCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGa    <  1:160563/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   cAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATAGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGCTCAACGGCGCTGACGTTTCCCCATGTGAGCGTGAcc  >  1:88820/1‑249 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    
GCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACC  >  NC_000913/65998‑66488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑