Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,6380TC97.7% 143.2 / ‑6.9 44intergenic (‑10/‑190)iclR/metHDNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (8/35);  minor base G (0/1);  total (8/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGT  >  NC_000913/4223399‑4223880
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
ccaaccttcctcaccaaaCCCTGCTGTTGCATCGGTTTTATCATGCGGTGAGTCGTGGAATTGGGTAACCCGGCTTGTTGCGTCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGATCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATTGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTAt                                                                                                                                                                                                                                         <  1:87656/236‑1 (MQ=255)
       gactgaCGAACCCCTGTTTTTTCATCGTGGTTAGCAGGCGGTGGGTCGGGGAATTGGGTAGCCCGGCTGGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGaa                                                                                                                                                                                                                                  <  2:207497/246‑1 (MQ=255)
       gACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGa                                                                                                                                                                                                                                   <  2:173641/250‑1 (MQ=255)
             cgagaccctgggtttGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAAGTGGGTAACCCGGCTTGGTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGGGGCAACGGCGGGTTTTCTGCCGCGTTTTGCGGGAATGGGTGCGACCAGG‑ACAGTCTCCTTTTTCTGTATCGTGGAAAtcatt                                                                                                                                                                                                                            <  2:410379/238‑1 (MQ=255)
              gaatcccTGCTGTTGCATTGTGGTTGGCAGGCGGTGGTGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACGGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAAtcattt                                                                                                                                                                                                                           <  2:26502/247‑1 (MQ=255)
                      gctgTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATcattttcatttt                                                                                                                                                                                                                    <  1:108048/250‑1 (MQ=255)
                         gtggCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTTCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGTGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTa                                                                                                                                                                                                                  <  2:380879/246‑1 (MQ=255)
                            tcATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGGGGTTTGCGGGGATGGGGGGGGCCATGGAC‑GGCTCCTTTTTTTTTATCGTGGAAAtcattttttttttttttttt                                                                                                                                                                                                             >  1:259532/2‑238 (MQ=255)
                             actcgttggtggcgggCGGTGGGACGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTACCGTTAGTGCCACACTGCCATTGGATTCGGGAATCCGCTCCAGTAATTTCGGGCCACGCGTTAAAGAGTGAACCTGTCCAGGCGCTGGTGCGGTGGCAACTGCGGGTTTTCTGCCGCGTTTTGCGGGAATGGATGCGACCATG‑CCAGGTTCCGTTTTCTTTATCGTGGAAATCATTTTCATTTTTATGGTTa                                                                                                                                                                                                            <  2:370350/237‑1 (MQ=255)
                                 gTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTAGCGTGGAAATCATTTTCATTTTTATTGTTAGCTa                                                                                                                                                                                                        <  1:163951/251‑1 (MQ=255)
                                          aGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAg                                                                                                                                                                                               <  1:343617/251‑1 (MQ=255)
                                                 gggTCGTGGAATTGGGTAACCCGGCTTGTTGCCCCAGTGTCTTGAGCGCCACACTGCCAATGGATTCGGCAATCCACTCGAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGGGGCAACGGCTGGTTTTCTGCTTTGTGTCGCGTGAATGGGTGCGATCATG‑TCAGTCTCGGTTGTCTGTATGGGGGAAATCATCTTCATTTTTATTGGTAggtacggcaatatttaatga                                                                                                                                                                                        <  2:281010/251‑20 (MQ=255)
                                                        ggAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGATGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCAGTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGAt                                                                                                                                                                                  <  1:112342/250‑1 (MQ=255)
                                                                  aaCCCGTCGTGTTGCGCCAGCTCCTTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCATTAATTTCAGGCCGCTCGTTAAAGGCTGATCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCTGGAATGGGTGCGTCCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGtt                                                                                                                                                                        <  1:401999/250‑1 (MQ=255)
                                                                       ggCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGtt                                                                                                                                                                  <  1:65328/251‑1 (MQ=255)
                                                                        gtttgttgCGCCAGTGCCGTGAGGGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCGGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCTTTTCGCGTGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCTTGGAAATCATTTTCATGTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTg                                                                                                                                                                 <  2:304897/249‑1 (MQ=255)
                                                                        gCTTGTGGCGCCAGTTCCTTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTg                                                                                                                                                                 <  2:467571/251‑1 (MQ=255)
                                                                           tgtttctccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCTCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGaa                                                                                                                                                               <  2:209497/243‑1 (MQ=255)
                                                                                 gcCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGTTTCGCGGGGATGGGTGGGGCCATG‑ACAGTCTCCTTTTTTTTTATCGTGGGAATCATTTTCATTTTTTTTTTTTGCTTATGCAATAGTTACTGAACTGATCCGCTGAGTTAATGTTGTACAAAtct                                                                                                                                                        >  1:198012/1‑251 (MQ=255)
                                                                                  ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTCGCGGGGAAGGGGGCGGCCCTG‑ACAGTCTCCTTTTTTTGTTTTGTGGGAATTATTTTCATTTTTTTTGTTAGCTAAATCAATAGTTACTGAACTGTTCCGATGAGATAATGTTGTACAAAtttc                                                                                                                                                       >  1:247572/1‑248 (MQ=255)
                                                                                   cAGTTCCGTGAGTGCCCCACTGCCATTGTATTCGGCCATCCTCTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAGCCTGTCCCGTCGTTGGTGCGGTGGCAACGGCTGGTTTTCTGCCTCGTTTCGCTGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCTTTCTCATTTTTATTGTTATCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAtctct                                                                                                                                                      <  2:267720/251‑2 (MQ=255)
                                                                                         cGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGc                                                                                                                                                <  1:375913/251‑1 (MQ=255)
                                                                                             aGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGGTTTGCGGGGATTGGGGCGGCCCAG‑ACAGACTCCTTTTTTTTTATCGTGGGAATCATTTTCATTTTTTTTTTTAGCTTATTCAATAATTTCTGAACTGATACGATGAGTTAATGtttaaaaaaaatcatattgcgggg                                                                                                                                            >  1:320115/1‑229 (MQ=255)
                                                                                                                  tcgtcAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGAt                                                                                                                        <  1:423470/246‑1 (MQ=255)
                                                                                                                   cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTGTACCACAGATGc                                                                                                                      <  1:380240/251‑1 (MQ=255)
                                                                                                                               ccAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATTCGATGAGTTAATGTTGAACAATTCTCATGTTGCGTGGTTGGCGCTTTTACCACAGATGCGTTTATGcta                                                                                                            <  1:114984/249‑3 (MQ=255)
                                                                                                                                  gTAATTTCAGTGCACGCGTTAATGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCCACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCGGTAGCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAAGGTTGAATAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATg                                                                                                       <  2:244988/251‑1 (MQ=255)
                                                                                                                                    aatctCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTTCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGt                                                                                                     <  2:208754/247‑1 (MQ=255)
                                                                                                                                         cAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTTGCGGGGATGGGGGGGGCCCTG‑ACAGGCTCCTTTTTTTGTATCGTGGGAATAATTTTTATTTTTATTTTTATCTAATTCAATAGTTACTGAACTGATCCGATGGGGTAATGTTTAACAAATTTCATGTTTCGTGGGGGTGGCTTTTTCCACCAAAGCGTTTTTGCCAGTATgggagagt                                                                                                >  1:208754/1‑245 (MQ=255)
                                                                                                                                             ccACGCGTTAAAGACGGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAAt                                                                                            <  2:21196/251‑1 (MQ=255)
                                                                                                                                                      aaaGTCTGAACCTTTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCTTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTATCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTaa                                                                                   <  1:161494/251‑1 (MQ=255)
                                                                                                                                                      aaaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTttatta                                                                                    <  1:372194/250‑1 (MQ=255)
                                                                                                                                                                cctttCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCTCGGGAAGGGGTTCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTTAACTGATCCGATTAGTTAATGTTGAACAAATCTCATTTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTg                                                                         <  1:28296/247‑1 (MQ=255)
                                                                                                                                                                       aGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTTCCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCgtgt                                                                  <  2:366635/251‑1 (MQ=255)
                                                                                                                                                                       aGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCgtgt                                                                  <  2:198012/251‑1 (MQ=255)
                                                                                                                                                                          cGCTGGTGCGTTGGCAACGGCGGGTGTGCTGTCGCGTTTGTCGGGAATGGGTTCGACCATG‑TCAGTCTCCTTTTTCTGTATCGTGGAAATCAGTTTCATTTTTATTGTGAGCTAATGCAATAGTTACTGATCTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCTCAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGGCgg                                                               <  2:129723/251‑1 (MQ=255)
                                                                                                                                                                                   ggTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAgtg                                                      <  1:127617/251‑1 (MQ=255)
                                                                                                                                                                                            ggcggGTATTCTGGAGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTGCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTTATGCAATAGTTACTGAACTGATCCGATGTGTTAATTTTGAAAAAATCTCATGTTGTGTTGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTCTGGTTTGTTGGATTTTTATTATATCTGGGTTGAGCGTGTCGGGAGCAATTGTGtgcagc                                              >  2:68042/1‑250 (MQ=255)
                                                                                                                                                                                                   tttCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTgg                                       <  1:451640/250‑1 (MQ=255)
                                                                                                                                                                                                     tCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGaa                                     <  1:392466/250‑1 (MQ=255)
                                                                                                                                                                                                        gCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGaaca                                   <  1:14437/249‑1 (MQ=255)
                                                                                                                                                                                                                    gggAATGGGTGCGACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCACGTGTGAGCAGCAAAGTGGAACAACTGCGTgcgccgt                     >  2:88637/1‑248 (MQ=255)
                                                                                                                                                                                                                                gACCATG‑ACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTa         <  2:259532/251‑1 (MQ=255)
                                                                                                                                                                                                                                        aCAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGt  >  2:77488/1‑251 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
CCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATG‑ACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGT  >  NC_000913/4223399‑4223880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑