| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 65,788 | A→T | intergenic (‑8/+67) | polB ← / ← araD | DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 65,788 | 0 | A | T | 94.3% | 101.3 / ‑6.0 | 35 | intergenic (‑8/+67) | polB/araD | DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (10/23); total (11/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
ACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATG > NC_000913/65548‑66027 | cccGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCaaaa < 1:239095/250‑1 (MQ=255) ggCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCaaaaaaa < 1:386700/251‑2 (MQ=255) gTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGACCCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTg < 1:340101/251‑1 (MQ=255) ggggTGCCAGGCGAACGCCTTTTTCACCCTGCAAAAAAGGCTGAGCGCGGGGACCCTGATCGGGGGGAATAAACGCCACGGACCC‑TTGCGGGGCAAGCGTATCCTGCAACGGCCCGTTGCCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGttt < 2:285959/249‑1 (MQ=255) gtgtCAGGCGAACGCCTTGTTCACCCTGCAAAACTTGCTGAGCGCGGGGACCCTGATCGGCGGGAATAAACGCCACGTACTC‑TTGCGGTGCAAGCGTAACCGGCAACGGCCCGTTGTCCGCCGCCAGCCAGAAGGAGACTTCTGTCCTTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATCGCCTGGTTTCGTTTGAt < 1:103446/251‑1 (MQ=255) cAGGCGATCGCCTTGTTCCCCCGGCAAAATTTGCGGGGCGCGGGGAACCTGTACGGCGGTAATAACCGCCCCGGACTC‑TTGCGGTGCAAGCGTAACCGGCAGCGGCCCGTTGCCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCGCGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGc < 2:221559/251‑1 (MQ=255) gaacgCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAAACTGCAAAGGCCCGGTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAAACTGCCTGCGGCCCGCTGTAAATTCATCAAAAAAACAGGCTTGGTTATAGCCTGGTTTCGTTTGGTTGGCTGTgg > 1:246746/5‑251 (MQ=255) cACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAAAAAACGCCACGGACTC‑TTGCGGGGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑GTAAGGTAAAAACTGCCCGCGCCACGCTGTAATTCCCTTAAAAAACCAGGCTTGGTTATAGCCTTGTTTTGGTTGATTTGCTGTGGGTTTATATAGtt > 1:393670/1‑250 (MQ=255) aaTATGTTGAGCCCGGGGACCCTGCCCGGCGGGAATAACCGCCACGGACTC‑TGGGGGGGCAAGCTTACCCTGCAACGGCCGTTTGTCCGCCGCCAGCCAAAAGGAGATTTCTGTCCCTTGCGGGGTTCCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcct < 2:72002/251‑2 (MQ=255) gCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAGAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTaa < 1:107258/250‑1 (MQ=255) gAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCCCGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAata < 1:143898/249‑1 (MQ=255) ggggACCCGGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTACCCTGCACCGGCCCGTTGTCCGTCCCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTcg < 1:229693/251‑1 (MQ=255) gggAACCTGATCGGCGGGAATAACCGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTgg < 1:110015/214‑1 (MQ=255) gggAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCGCCAGTGTCGGG‑GTAAGATAAAAACTGCCTTCGCCACGCTGGAATTCCATCAAAAAACCAGGCTTGATTTTAGCCTGGGTTCGTTTGGTTGGCTGTGGTTTTTTACAATCAtttattcccggaaaattgctttgc > 1:323831/1‑229 (MQ=255) ggAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGGCCCGCCAGTGTCGGG‑TTTAGATAAAACCTGCCTGCGCCACCCTGGAATTCCCTCAAAAAAACAAGCTTGATTATAACCTGGGTTCGTTTTGTTGGATGTGGGTTTTTACAATCAtttcttccccgaaaatgcctttccc > 1:122855/1‑228 (MQ=255) aaCCTGATCGGCGGGAATAAACGCCACGGGCTC‑TTGCGGTGCAAGAGTAACCTGCCACGGCCCGGTGTCCGTCGCCAACCAGAAGGAGAATTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGGTTAA‑ATAAAAACTGCCTGCGCCACGCCGTAATTCCATCCAAAAACCAGGCTTGTTTTTAGCCTGGTTTTGTTTGGTTTGCTGGGGTTTTATAAAGTCATTAATGCCCGGAATATTCCTTCGCGcc > 1:358278/1‑251 (MQ=255) ataccgcgcgtaaTAAACCCCTCGGACCC‑TGGCGGTGCAAGCGTACCCTGCACCGGCCCGTGGGCCGCCGCCATCCAGAAGGAGACTGGTGTCCCTTGTGGGTGGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCGGTAATTCCATCAAAAAACCAGGCTTGATGATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGc < 2:64069/240‑1 (MQ=255) ggggAAAAAACGCCACGG‑CTCCTTGCGCGGCAAGCGTACCCTGCAACGGCCTGTGTTCCGTCGCAAGCCAGAAGGAGACTTCGGCCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCa < 2:323831/250‑1 (MQ=255) gggAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCTGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGc > 1:334298/1‑249 (MQ=255) aCTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGGGTTTATTcagc > 1:415002/1‑250 (MQ=255) gCGGTGCAAGCGTAACCGTCAACGCCCCGTTGTCCGTCGCCAGCCATAAGGAGCCTTCTGTCCCTTGCGGGGTTTCACGTCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACATTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGttt < 1:276648/251‑1 (MQ=255) gCAACGGCCCGTGGTCCGTCGACAGCCAGAAGGAGCTTTCTGTCCCTTGCGGGGTGTTCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCAACAAAAATCCAGGCTTGATTATAGCCTGGTGTCGTTTGATTGGCTGTGGTTTTATACAGGCATTACTGCCCGTAATATGTCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGt < 1:282102/247‑1 (MQ=255) cGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCCCGCCATGCTTACGCAGGTAGTTTTTTTTTAGCAGCGTTTGCTTCCTATCCGGTACATGCGGCGCt > 2:12742/1‑250 (MQ=255) gTTGTCCGCCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTaa < 1:460820/251‑1 (MQ=255) cggTCGCCAGCAAGAAGGAGACTTCTGCCCCTTGCGGGGTGTTCCGCCAGTGTCGGG‑TAAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCACAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAc < 1:415011/249‑1 (MQ=255) agaCTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑GTAAGATTAAACCTGCCTGCGCCCCGCCGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCCTAATATGCCTTCGCGCCATGCTTACGCCGATAGGGTTTTTTCAGCAGCGTTTGCTTGATATCCGGGAAATGCGGGGCTAACTTACGGCAGAATACCCCCatct > 2:47674/1‑249 (MQ=255) cccTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACctc < 1:354308/251‑1 (MQ=255) agggTGTCCCGCCAGTGTCGGG‑TTAAAATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGGTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATTCCTTCGCGCCATTCTTACGCAGAGTGTGTTTTTTTCGAAGCGTTTGATGCCTATCCGGTAACTTGGGCGCTAACCGACGGCAGAATATTCCCATATaaacctcctcctcccgc > 2:379824/2‑236 (MQ=255) cGCCAGTGTCGGG‑TTAAAATAAAACCTGCCTGCGCCACGCTGTAATTCCATCCAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCCTCGCGCCATGCTTACGCCGATAGTGTTTATTCAGCCGCGTTTGCTGCATATCCGGTAACTTCGGGGCTAACTTGCGGCCGAATTTACCCATATAAGCGACCTCTTCCCGCACGATGGCg > 2:128284/1‑251 (MQ=255) aGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTAt < 1:23030/251‑1 (MQ=255) gtCGGT‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCa < 2:378128/251‑1 (MQ=255) aaGAGAAAACCTGCCTGCGCAACGCTGTAATTCCATCAAAAAACCAGTCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCTTTACTGCCCGTAATATGCCTTCGCGCCATGTTTACTCAGATAGTGTTTATTCAGCATCGTGTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATAGCCCCATATAATCGACCTCTTCCAGCACGATGGCGTTATGCTCCGCagct < 1:425539/251‑4 (MQ=255) aaCCGGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTTGTGTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCGTCGCGCCATGCTTACGCAGATAGTGTTTATTCATCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAt < 1:433134/251‑1 (MQ=255) gcctgcGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAtttt < 1:12953/249‑1 (MQ=255) ccACGCTGTAAATCCAGCAAAAAACCAGGCTTGATTTTAGCCTGGTTTCGTTTGATTGGCGGTGGTTTTATACAGTCATTACTTCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGTCCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGTCATTTTTGcccatgc < 2:83784/251‑5 (MQ=255) | ACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATG > NC_000913/65548‑66027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |