Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,811	G→T	intergenic (‑31/+44)	polB ← / ← araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,811	0	G	T	100.0%	93.7 / NA	33	intergenic (‑31/+44)	polB/araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/22);  total (11/22)

GCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAAC  >  NC_000913/65579‑66032                                                                                                                                                                                                                                           |                                                                                                                                                                                                                              ggggTGCCAGGCGAACGCCTTTTTCACCCTGCAAAAAAGGCTGAGCGCGGGGACCCTGATCGGGGGGAATAAACGCCACGGACCC‑TTGCGGGGCAAGCGTATCCTGCAACGGCCCGTTGCCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGttt                                                                                                                                                                                                             <  2:285959/249‑1 (MQ=255)    gtgtCAGGCGAACGCCTTGTTCACCCTGCAAAACTTGCTGAGCGCGGGGACCCTGATCGGCGGGAATAAACGCCACGTACTC‑TTGCGGTGCAAGCGTAACCGGCAACGGCCCGTTGTCCGCCGCCAGCCAGAAGGAGACTTCTGTCCTTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATCGCCTGGTTTCGTTTGAt                                                                                                                                                                                                          <  1:103446/251‑1 (MQ=255)        cAGGCGATCGCCTTGTTCCCCCGGCAAAATTTGCGGGGCGCGGGGAACCTGTACGGCGGTAATAACCGCCCCGGACTC‑TTGCGGTGCAAGCGTAACCGGCAGCGGCCCGTTGCCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCGCGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGc                                                                                                                                                                                                      <  2:221559/251‑1 (MQ=255)             gaacgCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAAACTGCAAAGGCCCGGTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAAACTGCCTGCGGCCCGCTGTAAATTCATCAAAAAAACAGGCTTGGTTATAGCCTGGTTTCGTTTGGTTGGCTGTgg                                                                                                                                                                                                 >  1:246746/5‑251 (MQ=255)                         cACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAAAAAACGCCACGGACTC‑TTGCGGGGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑GTAAGGTAAAAACTGCCCGCGCCACGCTGTAATTCCCTTAAAAAACCAGGCTTGGTTATAGCCTTGTTTTGGTTGATTTGCTGTGGGTTTATATAGtt                                                                                                                                                                                     >  1:393670/1‑250 (MQ=255)                                   aaTATGTTGAGCCCGGGGACCCTGCCCGGCGGGAATAACCGCCACGGACTC‑TGGGGGGGCAAGCTTACCCTGCAACGGCCGTTTGTCCGCCGCCAGCCAAAAGGAGATTTCTGTCCCTTGCGGGGTTCCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGcct                                                                                                                                                                           <  2:72002/251‑2 (MQ=255)                                        gCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAGAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTaa                                                                                                                                                                       <  1:107258/250‑1 (MQ=255)                                           gAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCCCGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAata                                                                                                                                                                     <  1:143898/249‑1 (MQ=255)                                                 ggggACCCGGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTACCCTGCACCGGCCCGTTGTCCGTCCCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTcg                                                                                                                                                             <  1:229693/251‑1 (MQ=255)                                                  gggAACCTGATCGGCGGGAATAACCGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTgg                                                                                                                                                                                                 <  1:110015/214‑1 (MQ=255)                                                  gggAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCGCCAGTGTCGGG‑GTAAGATAAAAACTGCCTTCGCCACGCTGGAATTCCATCAAAAAACCAGGCTTGATTTTAGCCTGGGTTCGTTTGGTTGGCTGTGGTTTTTTACAATCAtttattcccggaaaattgctttgc                                                                                                                                                            >  1:323831/1‑229 (MQ=255)                                                   ggAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGGCCCGCCAGTGTCGGG‑TTTAGATAAAACCTGCCTGCGCCACCCTGGAATTCCCTCAAAAAAACAAGCTTGATTATAACCTGGGTTCGTTTTGTTGGATGTGGGTTTTTACAATCAtttcttccccgaaaatgcctttccc                                                                                                                                                           >  1:122855/1‑228 (MQ=255)                                                     aaCCTGATCGGCGGGAATAAACGCCACGGGCTC‑TTGCGGTGCAAGAGTAACCTGCCACGGCCCGGTGTCCGTCGCCAACCAGAAGGAGAATTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGGTTAA‑ATAAAAACTGCCTGCGCCACGCCGTAATTCCATCCAAAAACCAGGCTTGTTTTTAGCCTGGTTTTGTTTGGTTTGCTGGGGTTTTATAAAGTCATTAATGCCCGGAATATTCCTTCGCGcc                                                                                                                                                         >  1:358278/1‑251 (MQ=255)                                                         ataccgcgcgtaaTAAACCCCTCGGACCC‑TGGCGGTGCAAGCGTACCCTGCACCGGCCCGTGGGCCGCCGCCATCCAGAAGGAGACTGGTGTCCCTTGTGGGTGGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCGGTAATTCCATCAAAAAACCAGGCTTGATGATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGc                                                                                                                                                     <  2:64069/240‑1 (MQ=255)                                                                ggggAAAAAACGCCACGG‑CTCCTTGCGCGGCAAGCGTACCCTGCAACGGCCTGTGTTCCGTCGCAAGCCAGAAGGAGACTTCGGCCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCa                                                                                                                                              <  2:323831/250‑1 (MQ=255)                                                                 gggAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCTGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGc                                                                                                                                               >  1:334298/1‑249 (MQ=255)                                                                                  aCTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGGGTTTATTcagc                                                                                                                             >  1:415002/1‑250 (MQ=255)                                                                                         gCGGTGCAAGCGTAACCGTCAACGCCCCGTTGTCCGTCGCCAGCCATAAGGAGCCTTCTGTCCCTTGCGGGGTTTCACGTCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACATTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGttt                                                                                                                      <  1:276648/251‑1 (MQ=255)                                                                                                           gCAACGGCCCGTGGTCCGTCGACAGCCAGAAGGAGCTTTCTGTCCCTTGCGGGGTGTTCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCAACAAAAATCCAGGCTTGATTATAGCCTGGTGTCGTTTGATTGGCTGTGGTTTTATACAGGCATTACTGCCCGTAATATGTCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGt                                                                                                        <  1:282102/247‑1 (MQ=255)                                                                                                                    cGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCCCGCCATGCTTACGCAGGTAGTTTTTTTTTAGCAGCGTTTGCTTCCTATCCGGTACATGCGGCGCt                                                                                            >  2:12742/1‑250 (MQ=255)                                                                                                                     gTTGTCCGCCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTaa                                                                                          <  1:460820/251‑1 (MQ=255)                                                                                                                          cggTCGCCAGCAAGAAGGAGACTTCTGCCCCTTGCGGGGTGTTCCGCCAGTGTCGGG‑TAAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCACAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAc                                                                                     <  1:415011/249‑1 (MQ=255)                                                                                                                                            agaCTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑GTAAGATTAAACCTGCCTGCGCCCCGCCGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCCTAATATGCCTTCGCGCCATGCTTACGCCGATAGGGTTTTTTCAGCAGCGTTTGCTTGATATCCGGGAAATGCGGGGCTAACTTACGGCAGAATACCCCCatct                                                                   >  2:47674/1‑249 (MQ=255)                                                                                                                                                      cccTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACctc                                                         <  1:354308/251‑1 (MQ=255)                                                                                                                                                             agggTGTCCCGCCAGTGTCGGG‑TTAAAATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGGTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATTCCTTCGCGCCATTCTTACGCAGAGTGTGTTTTTTTCGAAGCGTTTGATGCCTATCCGGTAACTTGGGCGCTAACCGACGGCAGAATATTCCCATATaaacctcctcctcccgc                                                  >  2:379824/2‑236 (MQ=255)                                                                                                                                                                      cGCCAGTGTCGGG‑TTAAAATAAAACCTGCCTGCGCCACGCTGTAATTCCATCCAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCCTCGCGCCATGCTTACGCCGATAGTGTTTATTCAGCCGCGTTTGCTGCATATCCGGTAACTTCGGGGCTAACTTGCGGCCGAATTTACCCATATAAGCGACCTCTTCCCGCACGATGGCg                                         >  2:128284/1‑251 (MQ=255)                                                                                                                                                                          aGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTAt                                     <  1:23030/251‑1 (MQ=255)                                                                                                                                                                             gtCGGT‑TTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCa                                  <  2:378128/251‑1 (MQ=255)                                                                                                                                                                                      aaGAGAAAACCTGCCTGCGCAACGCTGTAATTCCATCAAAAAACCAGTCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCTTTACTGCCCGTAATATGCCTTCGCGCCATGTTTACTCAGATAGTGTTTATTCAGCATCGTGTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATAGCCCCATATAATCGACCTCTTCCAGCACGATGGCGTTATGCTCCGCagct                          <  1:425539/251‑4 (MQ=255)                                                                                                                                                                                             aaCCGGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTTGTGTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCGTCGCGCCATGCTTACGCAGATAGTGTTTATTCATCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAt                   <  1:433134/251‑1 (MQ=255)                                                                                                                                                                                                  gcctgcGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAtttt                <  1:12953/249‑1 (MQ=255)                                                                                                                                                                                                         ccACGCTGTAAATCCAGCAAAAAACCAGGCTTGATTTTAGCCTGGTTTCGTTTGATTGGCGGTGGTTTTATACAGTCATTACTTCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGTCCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGTCATTTTTGcccatgc       <  2:83784/251‑5 (MQ=255)                                                                                                                                                                                                             gctgtaattCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCaaat  <  1:163546/243‑2 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                              GCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTC‑TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGG‑TTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAAC  >  NC_000913/65579‑66032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑