Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,593	C→T	P396P (CCG→CCA)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,593	0	C	T	96.2%	62.9 / NA	27	P396P (CCG→CCA)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (13/12);  minor base G (0/1);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACC‑GAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCG  >  NC_000913/64349‑64826                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            cgcgCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCggcggc                                                                                                                                                                                                                                       <  1:1429/251‑1 (MQ=255)   cgcCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGCTCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGACAGGTGCGGATGCTCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCCCGTAGCCGCCCGGGCCGGCGTGTggcggccc                                                                                                                                                                                                                                     >  2:129183/1‑249 (MQ=255)             aGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCg                                                                                                                                                                                                                              >  1:376570/1‑248 (MQ=255)                   tAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGTTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAgg                                                                                                                                                                                                                      <  1:128054/250‑1 (MQ=255)                              cccGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCAGCGAGAAACCCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAAcc                                                                                                                                                                                                          <  2:146533/250‑1 (MQ=255)                                 tgcaggcaATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAgc                                                                                                                                                                                                       >  1:68618/2‑251 (MQ=255)                                         aTGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGGGCTCTGGATCAGGATGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCa                                                                                                                                                                                               <  2:340605/251‑1 (MQ=255)                                             ttttCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCAtac                                                                                                                                                                                            <  1:276107/250‑3 (MQ=255)                                                      gagaACCAGGCATCGAGAAAACCTTCGGTACTGGGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAAGCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATa                                                                                                                                                                                  <  2:218783/251‑1 (MQ=255)                                                         aaCCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCgag                                                                                                                                                                                                                            >  1:195651/1‑206 (MQ=255)                                                         aaCCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCgag                                                                                                                                                                                                                            <  2:195651/206‑1 (MQ=255)                                                          aCCAGGCATCGAGAAAACCTTGGGTACTGGGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGGGCGGATTATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAgaga                                                                                                                                                                              <  2:399455/251‑1 (MQ=255)                                                                       aaaaCCTTCGGTACTGTGATCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACgccg                                                                                                                                                                 >  1:198567/1‑251 (MQ=255)                                                                                               aacAGGCTGCGCCATGCCGTCCACCAGCCCGGCGGGATCAATCAGAAAGGGGCGGATGAACGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTcta                                                                                                                                        <  2:171170/249‑3 (MQ=255)                                                                                                    gCTGCGCAAGGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGAGGAGCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAAGCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTTGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGTTCTACCGGc                                                                                                                                   <  2:429252/251‑1 (MQ=255)                                                                                                         gcCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCCGCACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGACAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGCTGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGcc                                                                                                                              >  1:59492/1‑251 (MQ=255)                                                                                                           cATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGCTGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGACGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTTGGCGCGACATAACCAGCGCGGTGCATCAGAGGAAAATAGAGATGACCCAACGCC‑CAACCCGATCCCCAGTGTCGGGCTACCGGCAGGCCgg                                                                                                                            >  2:195725/1‑250 (MQ=255)                                                                                                                   ccaccaGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCGTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGtc                                                                                                                    <  2:167099/251‑2 (MQ=255)                                                                                                                                          aGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGAGGTGAATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGGGGCGGGCTACCGGCAGGCCGGTCACCGTCGGCCGTTCGAGGAAAAAAGGCAt                                                                                             >  2:41186/1‑251 (MQ=255)                                                                                                                                                                          tttaaagtccggacccagtaccgAATCATAAAGCCCGGGCCGTGAATCCATCACGTGGCCGCCAGGGCTGGCGTGGGGCGGCAATTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGGTCGAGTAAAAATGGCATGATTTCAGTGTTATGGAAAATCTGCGTCAcc                                                              <  2:220103/228‑1 (MQ=255)                                                                                                                                                                                        ccagtaccgAATCATAAAGCCCTGGCCGTGAATCCATCAAGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCCGCGCGGTGCATACGAGGAAAATAGCGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCAACCGGCAGGCCGTTCACCGTCGATCGTTAGAGTAAAAATGGCATGATTTCAGTTTTATGGGAAATCTGAGTCACCAGCT‑CGCAATCTTTc                                              >  1:324921/9‑251 (MQ=255)                                                                                                                                                                                                       aaaGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGGGTAAAAATAGCCTGATTTCCGTTTTATGGAAAATCTTCGTCACCAGCT‑CGCAATCTTTTCGGTTTTAAGTCGcc                               >  2:45512/1‑251 (MQ=255)                                                                                                                                                                                                         aGCCCGGGCCGTGAATCCATCCCGTAGCCGCCAGGGCTGTCGTGTGGCGGCACTTCGCCGAGAGCAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAg                             <  2:174818/251‑1 (MQ=255)                                                                                                                                                                                                                  cGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGttt                    <  2:451883/251‑1 (MQ=255)                                                                                                                                                                                                                    tGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCAACCGGCAGGCCGGTCACCGTCGGTCCTTCGGGTGAAAATGGCAGTATTTCAGTTTTATGGAAAATTTGCGTCACCATCC‑CGCTCACTTTCAGGCTATAAGtaccccacgccggtttat                  >  2:371396/1‑234 (MQ=255)                                                                                                                                                                                                                                  aGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGGGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCCATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAAcgg    >  1:242779/1‑251 (MQ=255)                                                                                                                                                                                                                                    ccgccAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACC‑GATCCCCCGTGGCGGGCTACCGGCAGGGCGTTCACCGTCGCACGTTCGAGTAAAAATTGCATGATTTTAGTTTTATGGGAAATATTCGTCCACAGCTACG‑AAACTTTCAGGTTATCAGTCGCCCGCGCAGGTTTTTATTTGGCGAAAcggcg  >  2:248861/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            CGCGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACC‑GAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCG  >  NC_000913/64349‑64826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑