breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 45,854 C→T H16H (CAC→CAT yaaU → putative transporter YaaU
RA 51,712 C→T G240E (GGA→GAA)  rsmA ← 16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase
RA 52,812 C→T R202H (CGC→CAC)  pdxA ← 4‑hydroxythreonine‑4‑phosphate dehydrogenase
JC JC 57,276 IS2 (+) +5 bp intergenic (‑167/‑84) lptD ← / → djlA lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA
RA 71,134 G→A G250S (GGT→AGT)  araC → DNA‑binding transcriptional dual regulator AraC
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 269,161 C→T intergenic (‑201/+128) ykfA ← / ← perR putative GTP‑binding protein YkfA/putative transcriptional regulator PerR
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
JC JC 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE
RA 631,633 G→A W580* (TGG→TGA cstA → pyruvate transporter CstA
RA 727,115 C→T A540T (GCA→ACA)  kdpA ← K(+) transporting P‑type ATPase subunit KdpA
RA 727,117 C→T G539D (GGC→GAC)  kdpA ← K(+) transporting P‑type ATPase subunit KdpA
RA 728,330 C→T V135M (GTG→ATG)  kdpA ← K(+) transporting P‑type ATPase subunit KdpA
RA 749,268 C→T G147E (GGA→GAA)  ybgP ← putative fimbrial chaperone YbgP
RA 750,845 C→T S442N (AGC→AAC)  ybgQ ← putative fimbrial usher protein YbgQ
RA 750,858 C→T D438N (GAT→AAT)  ybgQ ← putative fimbrial usher protein YbgQ
RA 759,426 G→A E241K (GAG→AAG)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 759,576 G→A G291S (GGC→AGC)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 784,462 C→T G121D (GGC→GAC)  zitB ← Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter
RA 787,273 C→T G108D (GGC→GAC)  gpmA ← 2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase
RA 790,185 C→T G282D (GGC→GAC)  galT ← galactose‑1‑phosphate uridylyltransferase
RA 793,306 C→T E161E (GAG→GAA modF ← ABC family protein ModF
RA 797,186 C→T G211G (GGC→GGT modC → molybdate ABC transporter ATP binding subunit
RA 802,526 C→T L214L (CTG→TTG)  ybhI → putative tricarboxylate transporter
RA 811,317 C→T L313F (CTT→TTT)  bioF → 8‑amino‑7‑oxononanoate synthase
RA 812,699 C→T L144L (CTG→TTG)  bioD → dethiobiotin synthetase
RA 816,122 C→T A176T (GCG→ACG)  ybhK ← putative transferase YbhK
RA 831,431 C→T S187F (TCC→TTC)  rhlE → ATP‑dependent RNA helicase RhlE
RA 850,617 C→T D56D (GAC→GAT ompX → outer membrane protein X
RA 869,690 G→A E94K (GAA→AAA)  gsiB → glutathione ABC transporter periplasmic binding protein
RA 874,129 C→T A384V (GCG→GTG)  pdeI → putative c‑di‑GMP phosphodiesterase PdeI
RA 884,712 G→A G347D (GGT→GAT)  mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 885,116 G→A P39S (CCT→TCT)  ybjH ← uncharacterized protein YbjH
RA 889,829 C→T intergenic (‑10/‑260) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 890,342 C→T P85L (CCG→CTG)  ybjM → putative inner membrane protein
RA 891,909 C→T intergenic (+3/‑58) nfsA → / → rimK NADPH‑dependent nitro/quinone reductase NfsA/ribosomal protein S6 modification protein
RA 896,433 C→T L100L (CTC→CTT potH → putrescine ABC transporter membrane subunit PotH
RA 904,702 C→T A37V (GCC→GTC)  ybjQ → putative heavy metal binding protein YbjQ
RA 908,889 C→T E136K (GAA→AAA)  ltaE ← low‑specificity L‑threonine aldolase
RA 910,080 C→T E324K (GAA→AAA)  poxB ← pyruvate oxidase
RA 915,852 C→T V66I (GTC→ATC)  aqpZ ← water channel AqpZ
RA 917,753 C→T R427R (CGC→CGT ybjD → DUF2813 domain‑containing protein YbjD
RA 921,399 C→T A351A (GCC→GCT macB → ABC‑type tripartite efflux pump ATP binding/membrane subunit
RA 921,756 C→T N470N (AAC→AAT macB → ABC‑type tripartite efflux pump ATP binding/membrane subunit
RA 937,458 C→T S29S (AGC→AGT lolA → outer membrane lipoprotein carrier protein
RA 943,967 C→T P185L (CCC→CTC)  dmsB → dimethyl sulfoxide reductase subunit B
RA 969,683 C→T L99F (CTT→TTT)  ycaQ → interstrand DNA crosslink repair glycosylase
RA 972,831 G→A P191S (CCG→TCG)  elyC ← envelope biogenesis factor
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 1,041,693 C→T R359R (CGC→CGT appA → periplasmic phosphoanhydride phosphatase/multiple inositol‑polyphosphate phosphatase
RA 1,057,826 C→T Q43Q (CAG→CAA torR ← DNA‑binding transcriptional dual regulator TorR
RA 1,069,024 C→T G272G (GGG→GGA rutG ← pyrimidine:H(+) symporter
RA 1,072,484 C→T G127D (GGC→GAC)  rutB ← ureidoacrylate amidohydrolase
RA 1,076,800 C→T A695T (GCG→ACG)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,077,916 C→T G323R (GGG→AGG)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,078,308 C→T G192D (GGT→GAT)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,086,382 C→T W46* (TGG→TGA pgaD ← poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaD
RA 1,095,379 C→T R20H (CGC→CAC)  insE4 ← IS3 element protein InsE
RA 1,098,239 C→T R118R (CGC→CGT ghrA → glyoxylate/hydroxypyruvate reductase A
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,111,819 C→T R319R (CGC→CGT opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,115,639 C→T intergenic (‑149/+23) mdtG ← / ← lpxL efflux pump MdtG/lauroyl acyltransferase
RA 1,116,975 C→T R57* (CGA→TGA)  trhO → tRNA U34 hydroxylase TrhO
RA 1,121,634 C→T S325N (AGC→AAC)  pyrC ← dihydroorotase
RA 1,125,203 C→T G42S (GGC→AGC)  mdtH ← multidrug efflux pump MdtH
RA 1,125,597 C→T R12R (CGC→CGT rimJ → ribosomal‑protein‑S5‑alanine N‑acetyltransferase
RA 1,128,392 C→T P185L (CCG→CTG)  murJ → lipid II flippase MurJ
RA 1,134,882 C→T P52S (CCG→TCG)  flgG → flagellar basal‑body rod protein FlgG
RA 1,135,255 C→T T176I (ACC→ATC)  flgG → flagellar basal‑body rod protein FlgG
RA 1,135,448 C→T Y240Y (TAC→TAT flgG → flagellar basal‑body rod protein FlgG
RA 1,164,563 G→A G157R (GGA→AGA)  nagZ → beta‑N‑acetylglucosaminidase
RA 1,167,940 C→T G114G (GGC→GGT ycfJ → PF05433 family protein YcfJ
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,255,644 G→A A103A (GCC→GCT ycgV ← putative autotransporter adhesin YcgV
RA 1,290,881 C→T L214F (CTT→TTT)  rssB → regulator of RpoS
MC JC 1,294,035 Δ141 bp [tdk] [tdk]
MC JC 1,299,499 Δ1,199 bp insH21 insH21
JC 1,303,073 Δ12 bp coding (175‑186/921 nt) oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,353,758 T→A D254V (GAT→GTT)  sapC ← putrescine ABC exporter membrane protein SapC
RA 1,494,388 Δ1 bp coding (684/924 nt) ydcI ← DNA‑binding transcriptional dual regulator YdcI
RA 1,517,508 G→T V40V (GTG→GTT ortT → orphan toxin OrtT
RA 1,564,294 C→T K480K (AAG→AAA dosP ← oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
RA 1,580,143 C→T T216T (ACG→ACA ydeM ← putative anaerobic sulfatase maturation enzyme YdeM
RA 1,591,418 C→T E253E (GAG→GAA hipA ← serine/threonine‑protein kinase toxin HipA
RA 1,593,422 C→T intergenic (‑980/+5195) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,598,229 C→T intergenic (‑5787/+388) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,605,649 C→T S200L (TCG→TTG)  lsrB → Autoinducer‑2 ABC transporter periplasmic binding protein
RA 1,627,000 C→T G127G (GGG→GGA dcp ← peptidyl‑dipeptidase Dcp
RA 1,628,875 C→T S175F (TCT→TTT)  rspR → DNA‑binding transcriptional repressor RspR
RA 1,637,739 G→A S15F (TCT→TTT)  gnsB ← protein GnsB
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,887 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,693,035 G→A A410V (GCC→GTC)  uidB ← glucuronide:H(+) symporter
RA 1,716,110 G→A S371S (TCC→TCT tyrS ← tyrosine‑‑tRNA ligase
RA 1,723,394 T→C V425A (GTG→GCG)  ydhK → putative transporter YdhK
RA 1,744,751 Δ1 bp coding (1295/1374 nt) mdtK → multidrug efflux pump MdtK
RA 1,784,535 G→A R520Q (CGG→CAG)  fadK → short chain acyl‑CoA synthetase
RA 1,789,888 G→A P453S (CCT→TCT)  selO ← protein adenylyltransferase SelO
RA 1,792,138 G→A intergenic (‑118/+129) rflP ← / ← nlpC anti‑FlhDC factor RflP/NlpC/P60 family lipoprotein NlpC
JC JC 1,792,772 IS2 (–) +5 bp intergenic (‑41/+33) nlpC ← / ← btuD NlpC/P60 family lipoprotein NlpC/vitamin B12 ABC transporter ATP binding subunit
RA 1,814,697 C→T F277F (TTC→TTT katE → catalase HPII
RA 1,834,800 G→A intergenic (+7/‑8) ydjY → / → ydjZ 4Fe‑4S ferredoxin‑type domain‑containing protein YdjY/DedA family protein YdjZ
RA 1,841,889 G→A A134T (GCG→ACG) 
L83L (CTG→TTG) 
nudG →
ynjH ←
5‑hydroxy‑CTP diphosphatase
DUF1496 domain‑containing protein YnjH
RA 1,844,327 G→A A182V (GCC→GTC)  ynjI ← DUF1266 domain‑containing protein YnjI
RA 1,890,220 G→A L105L (CTG→TTG)  yeaY ← Slp family lipoprotein YeaY
RA 1,911,287 G→A A338T (GCT→ACT)  yebQ → putative transporter YebQ
RA 1,914,184 G→A F211F (TTC→TTT prc ← tail‑specific protease
RA 1,918,760 G→A Q417Q (CAG→CAA letB → lipophilic envelope spanning tunnel
RA 1,944,191 G→A A260T (GCC→ACC)  znuB → Zn(2(+)) ABC transporter membrane subunit
RA 1,953,879 C→T H146H (CAC→CAT cmoB → tRNA U34 carboxymethyltransferase
RA 1,954,628 C→T G794S (GGC→AGC)  torZ ← trimethylamine N‑oxide reductase 2
RA 1,959,513 C→T E112K (GAA→AAA)  yecM ← putative metal‑binding enzyme YecM
RA 1,962,183 C→T A71V (GCT→GTT)  yecT → protein YecT
RA 1,964,451 C→T G200G (GGG→GGA flhA ← flagellar biosynthesis protein FlhA
RA 1,969,912 C→T G358D (GGC→GAC)  tap ← methyl‑accepting chemotaxis protein Tap
RA 1,970,990 C→T intergenic (‑6/+40) tap ← / ← tar methyl‑accepting chemotaxis protein Tap/methyl‑accepting chemotaxis protein Tar
RA 1,973,377 C→T A650T (GCG→ACG) 
A650T (GCG→ACG) 
cheA ←
cheA ←
chemotaxis protein CheA
chemotaxis protein CheA
RA 1,978,243 G→T intergenic (‑46/+275) flhD ← / ← insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
RA 2,003,264 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,242,121 A→G S283P (TCG→CCG)  yeiB ← DUF418 domain‑containing protein YeiB
JC JC 2,269,876 IS5 (+) +4 bp intergenic (+309/‑100) lpxT → / → mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
JC JC 2,319,850 IS2 (+) +5 bp coding (23‑27/2850 nt) rcsC ← histidine kinase RcsC
RA 2,397,444 G→A Q908* (CAA→TAA)  nuoG ← NADH:quinone oxidoreductase subunit G
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
JC JC 2,666,074 IS1 (+) +9 bp coding (640‑648/1281 nt) csiE → stationary phase‑inducible protein CsiE
RA 2,713,696 G→A W267* (TGG→TGA srmB → ATP‑dependent RNA helicase SrmB
RA 2,821,231 G→A R261C (CGC→TGC)  alaS ← alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
RA 3,018,330 C→T G757G (GGC→GGT ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,021,885 C→T C190C (TGC→TGT xdhD → fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,022,725 C→T F470F (TTC→TTT xdhD → fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,022,890 C→T N525N (AAC→AAT xdhD → fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,023,453 C→T S713F (TCC→TTC)  xdhD → fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,025,706 G→A Q452Q (CAG→CAA xanQ → xanthine:H(+) symporter XanQ
RA 3,036,530 C→T G526D (GGC→GAC)  recJ ← ssDNA‑specific exonuclease RecJ
RA 3,037,220 C→T R296Q (CGA→CAA)  recJ ← ssDNA‑specific exonuclease RecJ
RA 3,045,508 C→T G132S (GGC→AGC)  ygfF ← putative oxidoreductase YgfF
RA 3,047,801 C→T S414N (AGC→AAC)  gcvP ← glycine decarboxylase
RA 3,054,549 C→T A98T (GCC→ACC)  pepP ← Xaa‑Pro aminopeptidase
RA 3,061,743 C→T S298S (AGC→AGT scpA → methylmalonyl‑CoA mutase
RA 3,062,669 C→T A607V (GCC→GTC)  scpA → methylmalonyl‑CoA mutase
RA 3,079,623 C→T intergenic (‑293/+21) cmtB ← / ← tktA mannitol‑specific PTS enzyme IIA component CmtB/transketolase 1
RA 3,083,186 C→T V204V (GTG→GTA speB ← agmatinase
RA 3,088,390 T→C I36T (ATT→ACT)  galP → galactose:H(+) symporter
RA 3,099,793 G→A G312G (GGC→GGT ansB ← L‑asparaginase 2
RA 3,099,992 G→A S246F (TCC→TTC)  ansB ← L‑asparaginase 2
RA 3,100,526 G→A A68V (GCG→GTG)  ansB ← L‑asparaginase 2
RA 3,113,731 C→T D208N (GAT→AAT)  pppA ← prepilin peptidase
RA 3,115,541 G→A P1191L (CCG→CTG)  yghJ ← putative lipoprotein YghJ
RA 3,115,607 G→A T1169I (ACC→ATC)  yghJ ← putative lipoprotein YghJ
RA 3,116,497 G→A H872H (CAC→CAT yghJ ← putative lipoprotein YghJ
RA 3,117,082 G→A I677I (ATC→ATT yghJ ← putative lipoprotein YghJ
RA 3,118,246 G→A G289G (GGC→GGT yghJ ← putative lipoprotein YghJ
RA 3,120,989 G→A S97S (AGC→AGT glcA ← glycolate/lactate:H(+) symporter GlcA
RA 3,123,938 G→A G98G (GGC→GGT glcG ← putative heme‑binding protein GlcG
RA 3,124,449 G→A S337S (AGC→AGT glcF ← glycolate dehydrogenase, putative iron‑sulfur subunit
RA 3,125,051 G→A L137L (CTG→TTG)  glcF ← glycolate dehydrogenase, putative iron‑sulfur subunit
RA 3,126,605 G→A P473S (CCC→TCC)  glcD ← glycolate dehydrogenase, putative FAD‑linked subunit
RA 3,127,230 C→T L264L (CTG→CTA glcD ← glycolate dehydrogenase, putative FAD‑linked subunit
RA 3,133,041 G→A L58L (CTG→TTG)  yghR ← putative ATP‑binding protein YghR
RA 3,135,565 G→A N269N (AAC→AAT pitB ← metal phosphate:H(+) symporter PitB
RA 3,173,797 C→T E534K (GAG→AAG)  parE ← DNA topoisomerase IV subunit B
RA 3,191,118 C→T L163L (CTA→TTA)  yqiI → putative fimbrial protein YqiI
RA 3,227,310 G→A Q359Q (CAG→CAA ygjI → putative transporter YgjI
RA 3,239,627 G→A intergenic (+82/‑317) alx → / → sstT putative membrane‑bound redox modulator Alx/serine/threonine:Na(+) symporter
RA 3,268,970 A→G T186A (ACA→GCA)  yhaC → uncharacterized protein YhaC
RA 3,338,426 G→A I12I (ATC→ATT mlaD ← intermembrane phospholipid transport system, substrate binding protein MlaD
RA 3,395,215 G→A G348G (GGC→GGT yhdP ← phospholipid transport factor YhdP
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
MC JC 3,682,261 Δ34 bp coding (1154‑1187/1287 nt) dctA ← C4 dicarboxylate/orotate:H(+) symporter
JC JC 3,737,137 IS2 (+) +5 bp coding (37‑41/825 nt) bax ← putative glycoside hydrolase Bax
RA 3,782,932 C→T A244T (GCG→ACG)  gpsA ← glycerol‑3‑phosphate dehydrogenase
RA 3,809,561 C→T A341V (GCA→GTA)  waaA → KDO transferase
RA 3,849,102 C→T Q345Q (CAG→CAA uhpB ← sensory histidine kinase UhpB
RA 3,861,543 C→T intergenic (+367/+444) yidL → / ← glvB putative DNA‑binding transcriptional regulator YidL/putative PTS enzyme II component GlvB
RA 3,862,227 C→T G82G (GGG→GGA glvB ← putative PTS enzyme II component GlvB
RA 3,865,439 C→T D279N (GAT→AAT)  yidE ← putative transport protein YidE
RA 3,884,604 C→T R38C (CGC→TGC)  rnpA → RNase P protein component
RA 3,884,734 C→T P81L (CCG→CTG)  rnpA → RNase P protein component
RA 3,892,044 C→T P144S (CCA→TCA)  mdtL → efflux pump MdtL
RA 3,900,687 C→T E512K (GAA→AAA)  bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,921,759 C→T S98N (AGC→AAC)  atpB ← ATP synthase Fo complex subunit a
RA 3,923,235 C→T S149N (AGC→AAC)  rsmG ← 16S rRNA m(7)G527 methyltransferase
RA 3,928,607 C→T G333S (GGC→AGC)  viaA ← protein ViaA
RA 3,929,881 C→T G405S (GGC→AGC)  ravA ← regulatory ATPase RavA
RA 3,943,846 C→T noncoding (143/2904 nt) rrlC → 23S ribosomal RNA
RA 3,950,830 C→T intergenic (+273/‑1371) ilvX → / → ilvM uncharacterized protein IlvX/acetolactate synthase II subunit IlvM
RA 3,958,585 C→T L206L (CTG→TTG)  ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,985,672 G→A N112N (AAC→AAT aslA ← putative sulfatase AslA
RA 4,004,371 C→T L109L (CTG→CTA yigI ← putative thioesterase YigI
RA 4,012,224 C→T R198Q (CGA→CAA)  metR ← DNA‑binding transcriptional dual regulator MetR
RA 4,017,393 C→T L21L (CTG→TTG)  rmuC → putative recombination limiting protein RmuC
RA 4,042,505 C→T L31F (CTT→TTT)  srkA → stress response kinase A
RA 4,048,632 C→T S556F (TCT→TTT)  polA → DNA polymerase I
RA 4,060,522 C→T P26S (CCG→TCG)  yihL → putative DNA‑binding transcriptional regulator YihL
RA 4,078,229 C→T P261S (CCG→TCG)  fabY → putative fatty acid biosynthesis enzyme FabY
RA 4,099,855 C→T G224D (GGC→GAC)  rhaT ← rhamnose/lyxose:H(+) symporter
RA 4,100,529 C→T intergenic (‑4/‑281) rhaT ← / → sodA rhamnose/lyxose:H(+) symporter/superoxide dismutase (Mn)
RA 4,102,105 C→T A139V (GCT→GTT)  kdgT → 2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
JC JC 4,123,923 IS2 (–) +5 bp coding (530‑534/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
RA 4,129,874 C→T L14L (CTG→TTG)  metL → fused aspartate kinase/homoserine dehydrogenase 2
JC JC 4,135,180 IS2 (–) +5 bp coding (1346‑1350/2181 nt) katG → catalase/hydroperoxidase HPI
RA 4,146,389 (T)6→7 coding (132/879 nt) pflC → putative pyruvate formate‑lyase 2 activating enzyme PflC
RA 4,163,984 C→T L116F (CTT→TTT)  btuB → cobalamin outer membrane transporter
RA 4,187,918 C→T L857F (CTC→TTC)  rpoC → RNA polymerase subunit beta'
RA 4,192,374 C→T A88T (GCG→ACG)  thiG ← 1‑deoxy‑D‑xylulose 5‑phosphate:thiol sulfurtransferase
RA 4,199,021 C→T L78F (CTT→TTT)  nfi → endonuclease V
RA 4,231,162 G→A G270D (GGC→GAC)  rluF → 23S rRNA pseudouridine(2604) and tRNA(Tyr) pseudouridine(35) synthase
RA 4,232,133 C→T T367T (ACG→ACA lysC ← aspartate kinase III
RA 4,232,947 G→A T96I (ACT→ATT)  lysC ← aspartate kinase III
RA 4,233,152 G→A L28F (CTT→TTT)  lysC ← aspartate kinase III
RA 4,245,238 G→A I394I (ATC→ATT malE ← maltose ABC transporter periplasmic binding protein
RA 4,254,504 G→A L655L (CTG→TTG)  plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,264,068 G→A P55L (CCA→CTA)  qorA ← putative quinone oxidoreductase 1
RA 4,267,804 G→A A231T (GCC→ACC)  tyrB → tyrosine aminotransferase
RA 4,269,058 G→A intergenic (‑46/‑356) yjbS ← / → aphA uncharacterized protein YjbS/acid phosphatase/phosphotransferase
RA 4,269,459 G→A A16T (GCT→ACT)  aphA → acid phosphatase/phosphotransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,345,404 G→A A52V (GCC→GTC)  yjdF ← DUF2238 domain‑containing inner membrane protein YjdF
RA 4,346,287 G→A P347L (CCA→CTA)  fumB ← fumarase B
RA 4,349,573 G→A R154R (CGC→CGT dcuR ← DNA‑binding transcriptional activator DcuR
RA 4,356,135 C→T G93S (GGT→AGT)  dtpC ← dipeptide/tripeptide:H(+) symporter DtpC
RA 4,362,225 G→A intergenic (‑291/+326) cadC ← / ← pheU DNA‑binding transcriptional activator CadC/tRNA‑Phe
RA 4,363,123 G→A V62V (GTC→GTT yjdC ← putative DNA‑binding transcriptional regulator YjdC
RA 4,364,860 G→A Y61Y (TAC→TAT dsbD ← thiol‑disulfide exchange protein DsbD
RA 4,365,662 G→A S371F (TCT→TTT)  dcuA ← C4‑dicarboxylate transporter DcuA
RA 4,367,038 G→A I430I (ATC→ATT aspA ← aspartate ammonia‑lyase
RA 4,372,336 G→A V438M (GTG→ATG)  groL → chaperonin GroEL
RA 4,372,474 G→A E484K (GAA→AAA)  groL → chaperonin GroEL
RA 4,395,899 C→T C105C (TGC→TGT tsaE → N(6)‑L‑threonylcarbamoyladenine synthase, TsaE subunit
RA 4,397,434 C→T P8L (CCA→CTA)  mutL → DNA mismatch repair protein MutL
RA 4,397,478 C→T P23S (CCT→TCT)  mutL → DNA mismatch repair protein MutL
RA 4,405,925 C→T I413I (ATC→ATT purA → adenylosuccinate synthetase
RA 4,417,140 C→T P63P (CCC→CCT yjfP → carboxylesterase
RA 4,442,824 C→T S148F (TCC→TTC)  tamA → translocation and assembly module subunit TamA
RA 4,445,219 C→T Q370* (CAG→TAG)  tamB → translocation and assembly module subunit TamB
RA 4,447,409 C→T L1100F (CTT→TTT)  tamB → translocation and assembly module subunit TamB
RA 4,454,328 C→T A249V (GCC→GTC)  yjfF → galactofuranose ABC transporter putative membrane subunit YjtF
RA 4,456,542 C→T T253I (ACC→ATC)  mpl → UDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase
RA 4,472,059 C→T A113T (GCG→ACG)  pyrB ← aspartate carbamoyltransferase catalytic subunit
RA 4,486,822 C→T S202F (TCC→TTC)  lptF → lipopolysaccharide transport system protein LptF
RA 4,492,537 C→T intergenic (‑12/+50) idnT ← / ← idnO L‑idonate/5‑ketogluconate/gluconate transporter/5‑keto‑D‑gluconate 5‑reductase
RA 4,493,716 C→T A231T (GCG→ACG)  idnD ← L‑idonate 5‑dehydrogenase
RA 4,509,072 C→T pseudogene (64/471 nt) insO → IS911B regulator fragment
RA 4,510,361 C→T intergenic (+568/+329) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,510,711 C→T R249R (AGG→AGA fecE ← ferric citrate ABC transporter ATP binding subunit
RA 4,515,901 C→T E259E (GAG→GAA fecA ← ferric citrate outer membrane transporter
RA 4,517,868 C→T G123D (GGC→GAC)  fecI ← RNA polymerase sigma factor FecI
RA 4,519,033 C→T intergenic (+231/+305) insA7 → / ← yjhU IS1 family repressor protein InsA/putative DNA‑binding transcriptional regulator YjhU
RA 4,521,873 C→T V50I (GTC→ATC)  yjhF ← putative transporter YjhF
RA 4,524,922 G→A A30V (GCC→GTC)  yjhH ← putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase
RA 4,525,090 G→A L238L (CTC→CTT yjhI ← putative DNA‑binding transcriptional regulator YjhI
RA 4,529,317 C→T L309L (CTG→CTA sgcC ← putative PTS enzyme IIC component SgcC
JC JC 4,536,236 IS5 (+) +4 bp intergenic (‑2183/‑358) topAI ← / → ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
RA 4,542,683 Δ1 bp intergenic (+50/‑432) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit
MC JC 4,549,192 Δ39 bp coding (385‑423/903 nt) fimH → type 1 fimbriae D‑mannose specific adhesin
RA 4,552,055 C→T I140I (ATC→ATT uxuA → D‑mannonate dehydratase
RA 4,552,344 C→T P237S (CCG→TCG)  uxuA → D‑mannonate dehydratase
RA 4,560,376 C→T G102D (GGT→GAT)  yjiH ← uncharacterized protein YjiH
RA 4,566,967 C→T E94K (GAA→AAA)  yjiN ← DUF445 domain‑containing protein YjiN
RA 4,574,257 G→A intergenic (+326/+2655) yjiT → / ← mcrC putative uncharacterized protein YjiT/Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit
RA 4,579,530 C→T G104E (GGA→GAA)  symE ← nucleoid‑associated protein SymE

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196259 1211514 15256 9 [8] [8] 9 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270–1978503 9–776 45 [0] [0] 34 insB5–insA5 insB5,insA5

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)69 (1.090) 61/484 0.2 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 382183 NA (NA)noncoding (924/1331 nt) IS2 repeat region
* ? NC_000913 382179 =NA (NA)58 (0.920) 51/480 0.3 NA noncoding (920/1331 nt) IS2 repeat region
?NC_000913 = 382590 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 56677458 (0.910)25 (0.400) 16/480 2.2 31.0% pseudogene (91/93 nt) renD protein RenD
?NC_000913 568036 = 54 (0.860)pseudogene (2/213 nt) renD protein RenD
* ? NC_000913 = 1397238NA (NA)45 (0.710) 40/484 0.6 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)34 (0.530) 31/484 1.0 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL