Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,637,739	G→A	S15F (TCT→TTT)	gnsB ←	protein GnsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,637,739	0	G	A	93.8%	43.7 / NA	16	S15F (TCT→TTT)	gnsB	protein GnsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (6/9);  minor base C (1/0);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGCATTGGTTTAATAAAAACCATCAACAATTGTGATTTAGATATTCGGAACCATTCAAATATAACAAAACCCCGTAAAAACGAGGTTTATGGATAAATTTTATTATTGAATACATCAGATTAAATTAATCTTGACATCATAGCTTTCAAGACCCGTCATTTTTTCCCGTGCGGTAAACTGAATACTGGTAACTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAGAAATATCTGCTTCTGCTTTTGTTTTTAAGTTTTCAATATTCATCATTTCCTCTTTTAGTCTGTTATGACTTTCCAGTTACACAGTAAGTCGATTATATGGTGCAAACGTGTAAAAGATAAGATGAAACATCGCAATAATCAACATACGATAGTCTAAATTTTACACAAACAGACAAAGAGAATTTTCCTGAATTATCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTC  >  NC_000913/1637493‑1637980                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  aCTGCATTTGTTTAATAAAAACCATCAACAATTTTGATTTAGATTTTCGGAACCATTCAAATATAACAAAACCCCGTAAAAACGAGGTTTATGGATAAATTTTATTATTGAATACATCAGATTAAATTTATCTTGACATCATAACTTTCAATTCCCGTCATTTTTTCCCGTGCTGTAAACTTAATACTGGTAACTTCTTTCCCTGTCTTTTTCTTAATTTCAATAATTTTTTTTGTTATATATTCAAAAat                                                                                                                                                                                                                                               <  1:609785/251‑1 (MQ=255)    gCATTGGTTTAATAAAAACCATCAACAATTGTGATTTAGATATTCGGAACCATTCAAATATAACAAAACCCCGTAAAAACGAGGTTTATGGATAAATTTTATTATTGAATACATCAGATTAAATTAATCTTGACATCATAGCTTTCAAGACCCGTCATTTTTTCCCGTGCGGTAAACTGAATACTGGTAACTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATAtc                                                                                                                                                                                                                                            <  2:629213/251‑1 (MQ=255)        tGGTTTAATAAAAACCATCAACAATTGTGATTTAGATATTCGGAACCATTCAAATATAACAAAACCCCGTAAAAACGAGGTTTATGGATAAATTTTATTATTGAATACATCAGATTAAATTAATCTTGACATCATAGCTTTCAAGACCCGTCATTTTTTCCCGTGCGGTAAACTGAATACTGGTAACTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATAtctgct                                                                                                                                                                                                                                        >  2:546065/1‑251 (MQ=255)            ttAATAAAAACCATCAACAATTGTGATTTAGATATTCGGAACCATTCAAATATAACAAAACCCCGTAAAAACGAGGTTTATGGATAAATTTTATTATTGAATACATCAGATTAAATTAATCTTGACATCATAGCTTTCAAGACCCGTCATTTTTTCCCGTGCGGTAAACTGAATACTGGTAACTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATAtctgcttct                                                                                                                                                                                                                                     <  1:17235/250‑1 (MQ=255)              aataaAAACCATCAACAATTGTGATTTAGATATTCGGAAACAATCAAATATAACAAAACCCCGTAAAAACGAGGTTTATTGATAAATTTTATTATTGAATACATCAGATTAAATTAATCTTGACATCATAGCTTTCAAGACCCGTCATTTTTTCCCGTGCGGTAAACTGAATACTGGTAACTTCTTTCCCGGTCTTTTTCTGAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATAtctgcttctgct                                                                                                                                                                                                                                  <  2:539915/251‑1 (MQ=255)                    aaCCATCAACAATTGTGATTTAGATATTCGGAACCATTCAAATAAAACAAAACCCCGTAAAAACGAGGTTTATGGATAAATTTTATTATTGAATACATCAGATTAAATTAATCTTGACATCATAGCTTTCAAGACCCGTCATTTTTTCCCGTTCGGTAAACTGAATACTGGTACCTTCTTTCCCGGTCTTTTTCTTCAGTTCAATAATTTTTTTTGTTATATATTCACAAATCTCTGCTTCTGCTTTTGtt                                                                                                                                                                                                                            >  2:365680/1‑251 (MQ=255)                               aTTGTGATTTAGATATTCGGAACCATTCAAATATAACAAAACCCCGTAAAAACGAGGTTTATGGATAAATTTTATTATTGAATACATCAGATTAAATTAATCTTGACATCATAGCTTTCAAGACCCGTCATTTTTTCCCGTGCGGTAAACTGAATACTGGTAACTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATATCTGCTTCTGCTTTTGTTTTTAAGTTTTc                                                                                                                                                                                                                 <  1:195630/251‑1 (MQ=255)                                                               ataACAAAACCCCGTAATAACGAGGTTTATGGATAAATTTTATTATTGAGTTCATCAGATTAAATTAATCTTGACATCATAGCTTTCAATACCCGTCATTTTTTCCCGTGCGGTAAACTAAATACTGGTACCTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATCTCTGCGTCTGCTTTTGTTTTTAAGTTTTCAATATTCATCATTTCCTCTTTTAGTCTGTTa                                                                                                                                                                                  <  2:484884/250‑1 (MQ=255)                                                                      aaCCCCGTAAAAACGAGGTTTATGGATAAATTTTATTATTGAATACATCAGATTAAATTAATCTTGACATCATAGCTTTCAAGACCCGTCATTTTTTCCCGTGCGGTAAACTGAATACTGGTAACTTCTTTCACGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATATCTGCTTCTGCTTTTGTTTTTAAGTTTTCAATATTCATCATTTCCTCTTTTAGTCTGTTATGACTTTc                                                                                                                                                                          >  1:231525/1‑251 (MQ=255)                                                                        ccccGTAAAAACGAGGTTTATGGATAAATTTTATTATTGAATACATCAGATTAAATTAATCTTGACATCATAGCTTTCAAGACCCGTCATTTTTTCCCGTGCGGTAAACTGAATACTGGTAACTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATATCTGCTTCTGCTTTTGTTTTTAAGTTTTCAATATTCATCATTTCCTCTTTTAGTCTGTTATGACTTTcc                                                                                                                                                                         >  1:182094/1‑250 (MQ=255)                                                                                                                                taaTCTTGACATCATAGCTTTCAAGACCCGTCATTTTTTCCCGTGCGGTAAACTGAATACTGGTAACTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATATCTGCTTCTGCTTTTGTTTTTAAGTTTTCAATATTCATCATTTCCTCTTTTAGTCTGTTATGACTTTCCAGTTACACAGTAAGTCGATTATATGGTGCAAACGTGTAAAAGATAAGATGAAACATc                                                                                                                >  1:362462/1‑251 (MQ=255)                                                                                                                                                             gTCATTTTTTCCCGTGCGGTAAACTGAATACTGGTAACTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATATCTGCTTCTGCTTTTGTTTTTAAGTTTTCAATATTCATCATTTCCTCTTTTAGTCTGTTATGACTTTCCAGTTACACAGTAAGTCGATTATATGGTGCAAACGTGTAAAAGATAAGATGAAACATCGCAATAATCAACATACGATAGTCTAAAtt                                                                                   <  1:539305/251‑1 (MQ=255)                                                                                                                                                                                 aaaCTGAATACTGGTAACTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATATCTGCTTCTGCTTTTGTTTTTAAGTTTTCAATATTCATCATTTCCTCTTTTAGTTTGTTATGACTTTCCAGTTACACAGTAAGTCGATTATATGGTGCAAACGTGTAAAAGATAAGATGAAACATCGCAATAATCAACATACGATAGTCTAAATTTTACACAAACAGACAAaga                                                                >  2:135334/1‑250 (MQ=255)                                                                                                                                                                                      gAATACTGGTAACTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAAAAATATCTGCTTCTGCTTTTGTTTTTAAGTTTTCAATATTCATCATTTCCTCTTTTAGTCTGTTATGACTTTCCAGTTACACAGTAAGTCGATTATATGGTGCAAACGTGTAAAAGATAAGATGAAACATCGCAATAATCAACATACGATAGTCTAAATTTTACACAAACAGACAAAGAGAAtt                                                           <  2:8858/250‑1 (MQ=255)                                                                                                                                                                                                                                  atTTTTTTTGTTATATATTCAAAAATATCTGCTTCTGCTTTTGTTTTTAAGTTTTCAATATTCATCATTTCCTCTTTTAGTCTGTCATGACTTTCCAGTTACACAGTAAGTCGATTATATGGTGCAAACGTGTAAAAGATAAGATGAAACATCGCAATAATCAACATCCGATATTCTAAATTTTACACAAACAGACAAAGAGAATTTTCCCGAATTATCAATGCAATAGCATCAAATCAACTCAAGAGCCt              <  2:231525/251‑1 (MQ=255)                                                                                                                                                                                                                                              atatatTCAAAAATATCTGCTTCTGCTTTTGTTTTTAAGTTTTCAATATTCATCATTTCCTCTTTTAGTCTGTTATGACTTTCCAGTTACACAGTAAGTCGATTATATGGTGCAAACGTGTAAAAGATAAGATGAAACATCGCAATAATCAACATACGATAGTCTAAATTTTACACAAACAGACAAAGAGAATTTTCCTGAATTATCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTc  >  1:587820/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  ACTGCATTGGTTTAATAAAAACCATCAACAATTGTGATTTAGATATTCGGAACCATTCAAATATAACAAAACCCCGTAAAAACGAGGTTTATGGATAAATTTTATTATTGAATACATCAGATTAAATTAATCTTGACATCATAGCTTTCAAGACCCGTCATTTTTTCCCGTGCGGTAAACTGAATACTGGTAACTTCTTTCCCGGTCTTTTTCTTAAGTTCAATAATTTTTTTTGTTATATATTCAGAAATATCTGCTTCTGCTTTTGTTTTTAAGTTTTCAATATTCATCATTTCCTCTTTTAGTCTGTTATGACTTTCCAGTTACACAGTAAGTCGATTATATGGTGCAAACGTGTAAAAGATAAGATGAAACATCGCAATAATCAACATACGATAGTCTAAATTTTACACAAACAGACAAAGAGAATTTTCCTGAATTATCAATGCAATAGCATCAAATCAACTCAAGAGCCTTATTGCTGCTTC  >  NC_000913/1637493‑1637980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑