Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,509,072	C→T	pseudogene (64/471 nt)	insO →	IS911B regulator fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,509,072	0	C	T	100.0%	211.6 / NA	64	pseudogene (64/471 nt)	insO	IS911B regulator fragment
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/28);  total (36/28)

GAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGGTGTGGTGCGGTGATGTGACCTATATC  >  NC_000913/4508834‑4509317                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       gAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGcc                                                                                                                                                                                                                                            >  2:540066/1‑251 (MQ=255)         aaTCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCTGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTATGATGCCATTCTAGTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGtta                                                                                                                                                                                                                                     <  1:308302/250‑2 (MQ=255)            cGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGcc                                                                                                                                                                                                                                 >  2:572852/1‑251 (MQ=255)                           cTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATc                                                                                                                                                                                                                  >  2:66598/1‑251 (MQ=255)                             gATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCg                                                                                                                                                                                                                 <  1:214446/250‑1 (MQ=255)                                  aaaGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGCGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCcaca                                                                                                                                                                                                            >  2:81272/1‑250 (MQ=255)                                  aaaGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCcaca                                                                                                                                                                                                            >  2:167639/1‑250 (MQ=255)                                            ttCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAAccc                                                                                                                                                                                                  <  1:418037/250‑1 (MQ=255)                                                ataaCCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGGTAAACTCAGGCTTCCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCAGCGTCACAATGGCAACCCGtaga                                                                                                                                                                                             <  2:106637/249‑1 (MQ=38)                                                     cGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCt                                                                                                                                                                                         >  2:617108/1‑250 (MQ=255)                                                      gTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTAc                                                                                                                                                                                       <  1:167639/251‑1 (MQ=255)                                                        tCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTAcc                                                                                                                                                                                      >  1:20819/1‑250 (MQ=255)                                                          tGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTAcc                                                                                                                                                                                      >  2:158352/1‑248 (MQ=255)                                                           gACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGAt                                                                                                                                                                                  <  1:128304/251‑1 (MQ=255)                                                                                tttAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCa                                                                                                                                                             <  2:172367/250‑1 (MQ=255)                                                                                 ttAGAGTAACGTTGGGTCTTTCCCATCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGCAGTGTCCGTTAATATCAGGCTACCTCAGGAAAAACCATCCTGAATAACCAGACGGAAGACGGGCTGTATTACGCAGTAAGGAAATTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGAAAGAAGCATCGCAACAATGGCAACCAGGAGACGCTACGAGATGGGCAC‑CTGGCCTGCTGGCacg                                                                                                                                                           >  2:421794/1‑249 (MQ=255)                                                                                    gagTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACACTGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTc                                                                                                                                                        >  2:402512/1‑251 (MQ=255)                                                                                      gTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCAt                                                                                                                                                      >  2:151200/1‑251 (MQ=255)                                                                                        aaCGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATAAGCCACGGTTCGGACGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCTGCTGGG‑ACGCTGGCTCGCTGGCAGGCTCATgg                                                                                                                                                    >  2:58194/1‑250 (MQ=255)                                                                                           gTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGaaa                                                                                                                                                  >  2:505092/1‑250 (MQ=255)                                                                                              gggTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGc                                                                                                                                              <  1:81272/251‑1 (MQ=255)                                                                                                  ttttCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCGCATTAAAGAGCAggg                                                                                                                                          >  2:339131/1‑251 (MQ=255)                                                                                                        gCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACTCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCt                                                                                                                                       <  1:111622/248‑1 (MQ=255)                                                                                                        gCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCt                                                                                                                                       <  1:558282/248‑1 (MQ=255)                                                                                                                 taGCTCTTTGTGATGCCATTCTATTTCAGGAAGGAGTGTTCGTTAAACTCAGGCTACACCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTACTACGCAGTCAGCTACTTGAGCTATATGGCATCAGCCACTGTTCGCCCGTCGCAAGAAGCATCGCCACAATGGCAACACGGAGAGGCTACCAGATGGG‑AAGCTGGCTTGCTGGCAGGCTCATGAAAGAGCCGGGGCTGTTCAGCTGTc                                                                                                                            >  2:359876/1‑250 (MQ=255)                                                                                                                  aGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACCCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGTTCAGCTGTcag                                                                                                                          <  1:400286/251‑1 (MQ=255)                                                                                                                          cTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGAc                                                                                                                  >  1:379626/1‑251 (MQ=255)                                                                                                                             aTGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTc                                                                                                                >  2:377705/1‑250 (MQ=255)                                                                                                                                    tCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGta                                                                                                         <  1:266236/250‑1 (MQ=255)                                                                                                                                           cAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAACGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACggg                                                                                                 >  2:332233/1‑249 (MQ=255)                                                                                                                                           cAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACgtg                                                                                                 >  2:453668/1‑251 (MQ=255)                                                                                                                                              gaaggaGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACgtggtg                                                                                              <  1:377705/251‑1 (MQ=255)                                                                                                                                                aggaGTTTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCTCCGTTATAAACGTggtggt                                                                                            <  2:482043/251‑1 (MQ=255)                                                                                                                                                aggaGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTggtggt                                                                                            >  2:385824/1‑251 (MQ=255)                                                                                                                                                        ccGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAAc                                                                                     >  1:527784/1‑250 (MQ=255)                                                                                                                                                        ccGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACa                                                                                    <  1:370511/251‑1 (MQ=255)                                                                                                                                                        ccGTTAAACTCAGGCTACCTAAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAAc                                                                                     >  2:584322/1‑250 (MQ=255)                                                                                                                                                         cGTTAAACGCAGGCGACCGCAGGAAAAACCGTCCTGAAAAACCAGACGGGAGACGGGCTGTATTTCGCAGTGAGGTACTTGAGCTATATGGCATCAGCCTCGGTTTGGCCGTATCATGAAGCATCGTCACCATGGCAACCCGGATAGGCTACCAGATGGG‑ACGCTCGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGCCAGCAGCCGACTCACCGGCATAAACGTGGTGGTCATGAACAt                                                                                   <  2:414798/251‑1 (MQ=255)                                                                                                                                                              aaCTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGGCATGAACATGTTGc                                                                              >  1:172197/1‑251 (MQ=255)                                                                                                                                                               aCTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCt                                                                             <  1:594094/251‑1 (MQ=255)                                                                                                                                                               aCTCAGGCTACCACAGGAAAAACCGTACTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACCCAACGGGATAAACGTGGTGGTCTTGAACATGTTGCt                                                                             >  2:447528/1‑251 (MQ=18)                                                                                                                                                                cTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCt                                                                             >  1:285429/1‑250 (MQ=255)                                                                                                                                                                  cAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATc                                                                          <  1:572852/251‑1 (MQ=255)                                                                                                                                                                       tACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGAAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCACCCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACCCACCGGCATAAACGTGGTGGTCATGAACCTGTTGCTATCCCTa                                                                      >  2:516513/1‑250 (MQ=255)                                                                                                                                                                        aCCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTa                                                                      <  2:172197/249‑1 (MQ=255)                                                                                                                                                                            cAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTAcc                                                                >  2:33503/1‑251 (MQ=255)                                                                                                                                                                                         ccTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGtt                                                   <  1:540066/251‑1 (MQ=255)                                                                                                                                                                                            gAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGGGGGGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGc                                                >  2:461815/1‑251 (MQ=255)                                                                                                                                                                                              aaaaCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCg                                              <  1:151200/251‑1 (MQ=255)                                                                                                                                                                                                  ccAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGAc                                          >  2:611206/1‑251 (MQ=255)                                                                                                                                                                                                   cagacggcagacggGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGAcc                                         <  1:402512/251‑1 (MQ=255)                                                                                                                                                                                                      acggcagacggGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAg                                      <  1:1042/251‑1 (MQ=255)                                                                                                                                                                                                        ggcagacggGCTGTATTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGCGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGACTCATGAAAGCGCTGAGGCTGGTCAGCTGTCAGCAGCCGACTCCCCGGtat                                                                                                        >  1:600417/1‑183 (MQ=17)                                                                                                                                                                                                        ggcagacggGCTGTATTACGCAGTCAGGTACTTGAGCTATAGGGCATCAGCCACGGTGCGTCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCATATGGG‑ACGCTGGCTTGCTGGCATGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCATCAGCCGACTCACCGGtat                                                                                                        <  2:600417/183‑1 (MQ=17)                                                                                                                                                                                                                   tGTATTACGCAGTCAGCTACTTGAGCTATGTGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCGGATGGG‑ACGCTGGCTTGCTGAAAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGGGGGCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGgtgg                         >  1:208891/1‑250 (MQ=21)                                                                                                                                                                                                                      aTTACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGGTgtggt                      >  1:23597/1‑251 (MQ=255)                                                                                                                                                                                                                      aTTACGCAGTCAGGCACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGGTgtggt                      >  1:525543/1‑251 (MQ=255)                                                                                                                                                                                                                        tACGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGGTGTGGTGc                    >  1:227086/1‑251 (MQ=255)                                                                                                                                                                                                                          cGCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGGTGTGGTGCgg                  >  2:571741/1‑251 (MQ=255)                                                                                                                                                                                                                           gCAGTCAGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGGTGTGGTGCGGt                 <  2:223525/251‑1 (MQ=255)                                                                                                                                                                                                                             aGTCAGGTACTTGAGCTCTATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGGTGTGGTGCGGTg                >  1:507723/1‑250 (MQ=255)                                                                                                                                                                                                                                 aGGTACTTGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGGTGTGGTGCGGTGAtgtg           <  1:505092/251‑1 (MQ=255)                                                                                                                                                                                                                                        tGAGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGGTGTGGTGCGGTGATGTGACCta      <  1:33503/249‑1 (MQ=255)                                                                                                                                                                                                                                          aGCTATATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGGTGTGGTGCGGTGATGTGACCTATATc  <  1:267242/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       GAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGGTTTTTCTGCTCATTATTAGCTCCTTCTGATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGG‑ACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTACCTTGAAAGGCAGTTCGCCGTGACCGAGCCAAATCAGGTGTGGTGCGGTGATGTGACCTATATC  >  NC_000913/4508834‑4509317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑