Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,115,639	C→T	intergenic (‑149/+23)	mdtG ← / ← lpxL	efflux pump MdtG/lauroyl acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,115,639	0	C	T	100.0%	109.5 / NA	33	intergenic (‑149/+23)	mdtG/lpxL	efflux pump MdtG/lauroyl acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/17);  total (16/17)

CAGACTGAAGGCGGCACCGGTAAGAAAACAGCCTAGCCAGGCGACGATCAGGTTTCGTTTCCAGTTTATAGGGGTGTCATTTTCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGC  >  NC_000913/1115395‑1115883                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     cAGACTGAACTCGGCACCGGTAAGAAAACAGCCTAGCCAGGCGACGTTAAGGTTTCGTTTCAAGTTTATAGGGGTGTCATTTTCACAGGGTGACATAGAAATCCGCTGTTGATGCGCCAGGCGAGGTGAACATATGATGAACAGATACGCCCACTAATTATGCGCCCGACTACCCGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGAGATTTGCTGTGGTAATGACGGCTGCCATTTTGAAGc                                                                                                                                                                                                                                                >  2:257799/1‑251 (MQ=255) cAGAATGAAGGCGGCACCGGTAAGAAAACAGCTTAGCTAGGAGACGCTCAGGTTTCGTTTTCAGTTTATAGGGGTGTCATTTTCACAGGGTGACATAGAAATCCGCTGATGGTGAGCCAGGCGCGGGGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGGAATCCAGAGAGATTTTATCGCTAAATCAGGGGGCTTTGCTGAGGTAATGCCGGCAGCCATTTTGAAGc                                                                                                                                                                                                                                                >  1:282633/1‑251 (MQ=255)                              aGCCTAGCCAGGCGACGATCAGGATTCGTTTCCAGTTTATAGGGGTGTCATTTTCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGCGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGAATTCCAGGGAGCGCAATCCAGAGAGATTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGACGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGa                                                                                                                                                                                                                    >  2:501017/1‑250 (MQ=255)                                        ggcggcGATCAGGTTTCGTTTCCAGTTTATAGGGGTGTCATTTTCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGGTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCtt                                                                                                                                                                                                         <  2:453907/251‑1 (MQ=255)                                                  aGGTTTCGTTTCCAGTTTATAGGGGTGTCATTTTCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTGTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCgtgt                                                                                                                                                                                               <  2:61730/251‑1 (MQ=255)                                                             ccAGTTTATAGGGGCGTCATTTTCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGCGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAc                                                                                                                                                                                    >  2:319335/1‑251 (MQ=255)                                                                gTTTATAGGGGTGTCATTTTCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGgt                                                                                                                                                                                 <  1:531204/251‑1 (MQ=255)                                                                   tataGGGGTGTCATTTTCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGCCGgtgt                                                                                                                                                                               <  2:338018/250‑1 (MQ=255)                                                                      aGGGGTGTCATTTTCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAAcc                                                                                                                                                                           <  1:30472/251‑1 (MQ=255)                                                                                  ttCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGct                                                                                                                                                               >  1:400297/1‑251 (MQ=255)                                                                                            gACATAGCAATCCGCTGTTGGTGCGCCAGGCGCTGTGAACATAAGAAGTAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGAGGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCca                                                                                                                                                      <  1:651730/250‑1 (MQ=255)                                                                                            gACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCcat                                                                                                                                                     <  2:170222/251‑1 (MQ=255)                                                                                                                    gcCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACttt                                                                                                                             <  1:120723/251‑1 (MQ=255)                                                                                                                       aGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGtt                                                                                                                          <  1:73795/251‑1 (MQ=255)                                                                                                                             ggTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCa                                                                                                                                                                                                                                               >  1:137268/1‑128 (MQ=255)                                                                                                                             ggTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCa                                                                                                                                                                                                                                               <  2:137268/128‑1 (MQ=255)                                                                                                                             ggTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCa                                                                                                                    <  1:267885/251‑1 (MQ=255)                                                                                                                                 aaCATAAGAAGAAAAGATAAGCACACTAATTATGCGTCCGACTTCCAGGGGGCGCAATCCAGTGAGCTTTTATCGCTAAATCAGGGGGATGTGCTTTGGTAATGCCGGATGCCAGTTTGAAGCAGCCGGCGTGGGAGATGTTATAGCGTGATGGAACTCCTTCCGGGCGGGTTTGAAAGCGGCAGTGTAACCAGATATCCTGTTCTGGTGTCCGCAGGATGCATTTTTCGACCGCTTTGTTCATCCAcgtg                                                                                                                <  2:547549/251‑3 (MQ=255)                                                                                                                                                    aGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGcatcat                                                                                             >  1:601072/1‑251 (MQ=255)                                                                                                                                                              ttATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACatat                                                                                                                                                                     <  2:69243/169‑1 (MQ=255)                                                                                                                                                              ttATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACatat                                                                                                                                                                     >  1:69243/1‑169 (MQ=255)                                                                                                                                                                     cccGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAacac                                                                            <  2:273882/251‑1 (MQ=255)                                                                                                                                                                     ccagACTTGCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCCGGGGGATTTGCTGTGGGAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAAGAGCGTGAAGGAACGCCTTCCGTGCGTGTTTTAGAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTGCGACCACTTTGTTCATCCACACGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAacac                                                                            <  2:531324/248‑1 (MQ=255)                                                                                                                                                                      ccGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGCTGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACAct                                                                           >  2:332427/1‑251 (MQ=255)                                                                                                                                                                                             agagagCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTg                                                     >  1:414568/1‑250 (MQ=255)                                                                                                                                                                                              gagagCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCAGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGATCTGGTGCCATCATGATGCATTTTTCGACCCCTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACCCTGGCGGCCGCATAATCAATTGAt                                                   >  2:617049/1‑251 (MQ=255)                                                                                                                                                                                                 agCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATAcc                                                >  1:243689/1‑251 (MQ=255)                                                                                                                                                                                                    ttttATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGGGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGACAACTCTGGCGGCAGCATAATCAATTTATACCCtt                                             >  2:523000/1‑251 (MQ=255)                                                                                                                                                                                                          cGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGAGGCGGAGAACACTCCGGCGGCAGCATAATCAATTGATAACCGTTGccct                                       >  2:459188/1‑249 (MQ=255)                                                                                                                                                                                                                  cAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCtt                                >  1:575783/1‑250 (MQ=255)                                                                                                                                                                                                                              cTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTg                                                                        >  1:203044/1‑198 (MQ=255)                                                                                                                                                                                                                              cTGTGGTAATGCCGGATGCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTg                                                                        <  2:203044/198‑1 (MQ=255)                                                                                                                                                                                                                                               gCCATTTTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATgc  <  1:211036/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     CAGACTGAAGGCGGCACCGGTAAGAAAACAGCCTAGCCAGGCGACGATCAGGTTTCGTTTCCAGTTTATAGGGGTGTCATTTTCACAGGGTGACATAGCAATCCGCTGTTGGTGCGCCAGGCGCGGTGAACATAAGAAGAAAAGATAAGCACACTAATTATGCGCCCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGC  >  NC_000913/1115395‑1115883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑