| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 750,845 | C→T | S442N (AGC→AAC) | ybgQ ← | putative fimbrial usher protein YbgQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 750,845 | 0 | C | T | 98.7% | 246.4 / NA | 76 | S442N (AGC→AAC) | ybgQ | putative fimbrial usher protein YbgQ |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (34/41); minor base A (1/0); total (35/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
GTCACCAATATCAACATTAAAACCTGCTGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCAT > NC_000913/750598‑751088 | gTCACCAATATCAACATTAAAAACTGCTGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGGAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGATGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAAAGACGATCTGAGAAGCGATAAGAGGCGAGCGAAATCGtgttgt > 1:497467/1‑251 (MQ=255) cAATATCAACATTAAAACCTGCTGTGATGTTGGCGGTCGGGGAGGCGTCCACAATCCACAGGGCTTGATGTAGCAGGGTGGCGTACAGATTGAGGTTTAGTGGGGTAATCGGCTGGCCCACGGATAAGCTGATCGTCTGCTTTTCGTCCTGCGCCTCGCTGTCGTTGTATTTGTGATCCCGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGACATCGTGATGAtggtg > 1:598431/1‑251 (MQ=255) cAATATCAACATTAAAACCTGCTGTGATGTTGGCGGTCGAGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGGAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGACCCAAGGATAAGCTGATCGTCTGTTTTTCGACCTGCGCATCGCTGGAGTTGTATTTGTGATCCAGGTAGTTGGCGTAGCTGTGAACATGACGATCTGGGAAGCGATAAGCGGCGTGCGAAATCGTGTTGTtggtg > 1:654315/1‑251 (MQ=255) cAACATTAAAACCTGCTGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCAttc < 2:225031/251‑3 (MQ=255) aaCATTAAAACCTGCTGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCAttc > 2:287446/1‑248 (MQ=255) aaaaCCTGCTGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGt < 1:229488/250‑1 (MQ=255) ccTGCTGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCt < 1:118383/251‑1 (MQ=255) gATGTTGGCGGTCGTGGAGGCGTCTTCATTCCACCAGGTTTGATGTAGCAGGTTGGCTTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTaa < 1:79573/251‑1 (MQ=255) ggTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGc < 1:327254/251‑1 (MQ=255) gTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTa > 2:611902/1‑250 (MQ=255) tGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTa > 1:515207/1‑249 (MQ=255) ggAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGc > 1:216907/1‑251 (MQ=255) aGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGccc > 1:632536/1‑251 (MQ=255) ggCGTATGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGACGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGTCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAACTCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAACACGGTAGCTTAAGcccc > 2:268643/1‑251 (MQ=255) gCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCg < 1:525306/249‑1 (MQ=255) cATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGAGTGAGGTTTAGTGGGGTAATCGGTGGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTGGTTTTCTGTAATTAAAACGGTAGCTTAAGCTCCGCTCGTca < 2:616363/251‑2 (MQ=255) ccaccaGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCctgct < 1:332366/251‑1 (MQ=255) accaGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATGCACTTGTTTGCTGTAATTAAAACTGTAGCTTAAGCCCCGCTCGTCCTGCTGa < 1:74249/251‑1 (MQ=255) aacaGGTTTGATGTAGCAGGATGGCGTAAAGATTGAGGTTTAGTAGGGTAATCGGATGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCctgctgg > 2:466719/3‑250 (MQ=255) caGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGt > 1:402257/1‑251 (MQ=255) ttGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCa > 1:338967/1‑250 (MQ=255) aTGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAAt > 2:520928/1‑251 (MQ=255) tGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCACAATGGCTACTGGcc > 2:484689/1‑251 (MQ=255) aaaGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTg < 1:95763/251‑1 (MQ=255) gTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAgg < 2:220694/251‑1 (MQ=255) aggggggTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGGTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAg < 2:233028/248‑1 (MQ=255) aGTGGGGTAATCTGTTGGCCCACGGATAAGCTGGTCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAg < 2:659753/251‑1 (MQ=255) gTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCTTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGt < 2:417124/251‑1 (MQ=255) gTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGt < 1:370172/251‑1 (MQ=255) ggggTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTgc < 1:558012/251‑1 (MQ=255) ggggTAATCGGGTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACGTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTg < 1:98582/250‑1 (MQ=255) gggTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTgcg > 1:506142/1‑251 (MQ=255) gTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTCCTGAGTATCAAAATGGCTACTGGCCCAGGCGACATCAAAGGACAGTGCGcc > 2:184364/1‑251 (MQ=255) aTCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAg < 1:559096/251‑1 (MQ=255) ggTTGGCCCACGGATAATCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGCCATCAAAGGACAGTGCGCCAAGccc > 2:95012/1‑250 (MQ=255) cccACGGATAAGCTGAGCGTCTGTTGTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGAGCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAAGTAAAACGGTAGCTTAAGCCCCGCTCGGCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCat < 1:85180/251‑1 (MQ=255) ggATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATAtttt < 1:611902/251‑1 (MQ=255) aaGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATGGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTTGCATTCACTTGTTTGCTGTAATTAAAACGGTTGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGcc < 2:546829/250‑1 (MQ=255) aTCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATAc > 1:349634/1‑250 (MQ=255) cGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATAccc < 1:184364/250‑1 (MQ=255) cGTCCTGCGCATCGCTGTCGTTGTACTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGaa < 1:35827/251‑1 (MQ=255) cGTCATGCGCATCGCTGTCGTTGTATGTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCCAGTGAAATCGTGTTGTTGGTGGCATTCCCTTGTTTGCTGTAATTAAACCGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAACGGCTACTGGCCCAGGTGACATCAAAGTACAGTGCCACAAGCCCCAGCATATTTTGCCCGATCCCCATTGCGGCAGa < 2:539761/250‑1 (MQ=255) ccTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATgg > 1:12319/1‑251 (MQ=255) gcgcATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTAACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGAGCCAAGCCACAGCATATTTTGCCCGATACCCATTGAGGCAGACTGGt > 1:287546/1‑249 (MQ=255) cATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTATATGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAGTCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAACGGACAGTGCGCAAAGCCACAGCGTATTTTGCACGATACCCATTGCGGCAGAATGGTAGtc > 1:34878/1‑250 (MQ=255) cTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGtcatcatc > 1:158160/1‑251 (MQ=255) cTGTCGTTGTATTTGTGATACAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGtcatcatc > 1:354420/1‑251 (MQ=255) gTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGtcatcatc > 2:54124/1‑249 (MQ=255) tGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATc > 1:450454/1‑251 (MQ=255) tATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATc > 1:204545/1‑249 (MQ=255) aTTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATcag < 2:506142/250‑1 (MQ=255) gATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGgccg < 2:402257/251‑1 (MQ=255) gATCCAGATAGTGGGCGTAGCTGTGAAAATGACGATCTGAGAAGAGATAAGCGGCGAGCGAAATCGTGTGGTTGGTTGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTACTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGAGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGgccg < 2:505787/251‑1 (MQ=255) aGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGt > 1:300293/1‑250 (MQ=255) ggCGTAGCTGTGAAAAGGCAGATCTGAGAAGGGATAAGAGTATAGCTAAATCGTGTTGTTGGGGGCATTCTCTTGTTTGATGTAAATAAAGCGGTAGCTTAAGCCCCTCACGTCCGGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGTGCCAAGCCACAGGATATTTTGCCCGATACCCAGTGCGGCAGAATGGTAGTGATCATCAGAAAGGAGCAGGCTGGCGTACAGCGAgg < 2:59357/251‑1 (MQ=255) cGTAGCTGTGCCAATGACGATCTGAGCAGCGATAAGCGGCGAGAGAAATCGAGTTGTTGATGGCATTCACTTGTTTGCTGTAATTAAAAAGGGAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAACTGGATACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATAGCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGAGAnggg > 2:586441/1‑249 (MQ=255) aGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGttt < 2:547591/251‑1 (MQ=255) gtgAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCAAAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTg > 1:6420/1‑248 (MQ=255) tgAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATAACCATTGCGGCCGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGATTgag > 1:646453/1‑249 (MQ=255) tGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATccc > 2:510817/1‑251 (MQ=255) gACGATCTGAGAAGCGATAAGCGGCGAGTGAAATCGTGTTGTTGGTGGCATTCACTTGTGTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTTGCCCAGGTTACATCAAAGGACAGTGCGCCAAGCCACAGTATATTTTGCCCGATTCCCATTGCGGCAGAATGGTATTCATCATCAGAAATCAGCAGGCCGCTGTACAGCGAGGTGTTTGAGAGCATtccc < 2:497467/251‑1 (MQ=255) acgcacttccgatcggATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATcccc < 2:562119/236‑1 (MQ=255) gagaAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGa < 2:528062/248‑1 (MQ=255) gagaAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAAc < 2:543957/251‑1 (MQ=255) gagaAGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAAc < 2:355935/251‑1 (MQ=255) aaGCGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTc < 1:22317/251‑1 (MQ=255) cGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCTCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCAtt < 2:216907/251‑1 (MQ=255) cGATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCAtt > 1:570540/1‑251 (MQ=255) gATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATcccc > 1:562119/1‑236 (MQ=255) gATAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACGTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTg < 2:570960/251‑1 (MQ=255) tAAGCGGCGAGCGAAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCt > 1:538868/1‑251 (MQ=255) gcgagcgaAATCGTTTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCTGGTGACATCAAAGGACAGTGCTCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAACCTTCATTGCTaaaaa < 1:24843/250‑1 (MQ=255) gagcgaAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTg < 1:355524/241‑1 (MQ=255) gagcgaAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTg > 2:355524/1‑241 (MQ=255) agcgaAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGt < 2:570540/251‑1 (MQ=255) cgaAATCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAACCGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGNttt > 2:592509/1‑251 (MQ=255) aaTCGTGTTGTTGGTGGCATTCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCAt < 1:520928/251‑1 (MQ=255) | GTCACCAATATCAACATTAAAACCTGCTGTGATGTTGGCGGTCGTGGAGGCGTCTGCATTCCACCAGGTTTGATGTAGCAGGTTGGCGTAAAGATTGAGGTTTAGTGGGGTAATCGGTTGGCCCACGGATAAGCTGATCGTCTGTTTTTCGTCCTGCGCATCGCTGTCGTTGTATTTGTGATCCAGATAGTTGGCGTAGCTGTGAAAATGACGATCTGAGAAGCGATAAGCGGCGAGCGAAATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCAT > NC_000913/750598‑751088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |