Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,032,423	G→A	V219V (GTC→GTT)	yedJ ←	putative HD superfamily phosphohydrolase YedJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,032,423	0	G	A	100.0%	178.0 / NA	56	V219V (GTC→GTT)	yedJ	putative HD superfamily phosphohydrolase YedJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/25);  total (31/25)

AGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACACGCGCG  >  NC_000913/2032185‑2032667                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     aGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATtc                                                                                                                                                                                                                                          <  2:286159/251‑1 (MQ=255)            ccTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTccccgcccgt                                                                                                                                                                                                                               >  2:356960/1‑245 (MQ=255)             cTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGt                                                                                                                                                                                                                              >  1:388716/1‑251 (MQ=255)                       aaGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTg                                                                                                                                                                                                                     >  1:218612/1‑250 (MQ=255)                       aaGCTGCGCCACCAACGTTTTAACATCATAAATTGGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTg                                                                                                                                                                                                                     <  1:423086/250‑1 (MQ=255)                        aGCTGCTCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTg                                                                                                                                                                                                                     >  1:564565/1‑249 (MQ=255)                            gcgcCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTc                                                                                                                                                                                                               <  1:149298/251‑1 (MQ=255)                                      cGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACt                                                                                                                                                                                                     >  1:469267/1‑251 (MQ=255)                                            aaCATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGACACTGAGCTTCGCCATAAACTCGACt                                                                                                                                                                                                >  1:82111/1‑250 (MQ=255)                                            aaCATCAAAAATTTGCAGCATTTTCACCAGCATTGCCTGGGCGACATTCCCGGTGCTGTATGAATCGGTTACTGAGATATTTGCCTGCATAGATGTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGAGGAAAACGCATCTATAACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACt                                                                                                                                                                                                <  1:30071/250‑1 (MQ=255)                                               atcatAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑CCAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTaaaa                                                                                                                                                                                            <  1:570043/251‑1 (MQ=255)                                                  atAAATTTGCAGCCGCTTGTCCAGAATTCCCTGTGCGTCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCCCCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACCGCCATTTAGGG‑CCACTCGCTCAGCGAGCGGATGCAGACGCATCTATAACCTTGTGGTCACCACCTTCACTATCCCCCGCCACTTCGGCCCTGAGCTTCGCCACACACTCGACTaaacaac                                                                                                                                                                                         >  2:33214/1‑247 (MQ=255)                                                     aaTTTGCAGCATTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGTTTACTGATATATTTTCCTGCATAGTTTTCACCGGCCTTCTGAGATGGCCG‑ACAGTTTAACATAATTCCAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACTCATCTATCACCTTGTGATAAACCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATgcg                                                                                                                                                                                      <  1:660814/251‑1 (MQ=255)                                                     aaTTTGCAGCAGTTTATACAGCATTGACTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTCCTGATATATTTTCCTCCATAGCTTTCACCGGCCATCTGAGATGGCAG‑AAAGGTTAACATAATTACAGCCATTTAGGG‑CCACACGCTCAGCCAGAGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCACCCTCCAGTTCGGCACTGAGCTTCACCACAAACTCGCCTAAACAATgcg                                                                                                                                                                                      >  2:418774/1‑251 (MQ=255)                                                      aTTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATgcgc                                                                                                                                                                                     >  1:14898/1‑251 (MQ=255)                                                                          cATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCGGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTGGTGACCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGGGCTGCGCCAgctgct                                                                                                                                                                 <  1:485511/251‑1 (MQ=255)                                                                                    gCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGc                                                                                                                                                       >  2:219304/1‑251 (MQ=255)                                                                                            cccGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGACATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGAACTGAGCTTCGCCATACACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGc                                                                                                                                                >  1:300019/1‑250 (MQ=255)                                                                                                 tGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCtt                                                                                                                                                                                                                                               <  2:112701/150‑1 (MQ=255)                                                                                                 tGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCtt                                                                                                                                                                                                                                               >  1:112701/1‑150 (MQ=255)                                                                                                  gctttATGAATCGTTTACTGATATTTTTTCCTGCATATATTTCACCGGCCATCTGATATGGCCG‑ACAGTTTAACATAATTCCAGCCATTTAGGG‑CCCCTCGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTGATCAACCCCTTCTTTCTCCCCCGCCAGTTCGTCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCTTTGTGCTGCGCCAGCTGCTTGCCTCTTGCTGTTTGCATGGtt                                                                                                                                          <  2:388716/246‑1 (MQ=255)                                                                                                             cGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTc                                                                                                                              >  1:314463/1‑251 (MQ=255)                                                                                                              ggTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTc                                                                                                                              >  2:99111/1‑250 (MQ=255)                                                                                                               gtgactaaaatatTTTCTTGCATAGATTTGCCCGGCCATCGGAGA‑GGCCGCACAGTTTATCATAATTACAGCC‑TTTAGGGGCCACGCTCTCCGTCAGCAGATAAAAACGCATCTGTCACCTGGGTATCAACCCCTGCATGCTCCCCCGCCAGTTCGGCAGTGAGCTTTGCCATAAACTCGACTAAAAAATGCGCGTTGTGAGGCGCCAGCTGCTTGCCTCTTTCGGTTTGGGTGGTTTGCGGTAGCTTCAg                                                                                                                            <  2:369113/242‑1 (MQ=255)                                                                                                                  aCTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCa                                                                                                                          >  1:193168/1‑250 (MQ=255)                                                                                                                  aCTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACACCCATTTAGGC‑CCTCTCGCTCATCCAGCGAATGAAAACGCATCTATCACCTTGTGATCAACTCCTCCATCCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTGTGCATGGTTTGCGGTAGCTTCAGCa                                                                                                                          <  1:652820/250‑1 (MQ=255)                                                                                                                     gATATATTTTCCTGCATAGATTCCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACt                                                                                                                       <  1:332167/250‑1 (MQ=255)                                                                                                                          atTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCt                                                                                                                 >  1:45769/1‑251 (MQ=255)                                                                                                                                     aaaaTTTCACCGGCCATCTGAGATGGCCG‑ACAGGTAAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATcc                                                                                                      <  2:45769/248‑1 (MQ=255)                                                                                                                                           tCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCCACTTAGTCTGGAAATGATCCAGcgcg                                                                                                >  1:574261/1‑251 (MQ=255)                                                                                                                                             aCCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGt                                                                                               >  1:261721/1‑250 (MQ=255)                                                                                                                                              ccGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAg                                                                                             <  1:259304/251‑1 (MQ=255)                                                                                                                                                         agaTGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTatcatc                                                                                  >  2:249837/1‑251 (MQ=255)                                                                                                                                                                ccgcaaGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGAGGAAACCCCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCgg                                                                            <  2:14898/246‑1 (MQ=255)                                                                                                                                                                         tAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATACAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCtg                                                                   >  2:425793/1‑251 (MQ=255)                                                                                                                                                                             aTAATTTCAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCg                                                               <  1:10011/251‑1 (MQ=255)                                                                                                                                                                                   aCAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACgg                                                          >  1:375734/1‑250 (MQ=255)                                                                                                                                                                                    cAGCCATTTAGGG‑CCACGCCCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGAt                                                        <  1:320231/251‑1 (MQ=255)                                                                                                                                                                                     cgCCATTTAGGG‑CCCCGCGCTCAGCCAGCGGATGAAAACGCTTCTATCCCCTTGGTATCAACCCCTCCATTCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATc                                                       <  2:314463/250‑1 (MQ=255)                                                                                                                                                                                       ccATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCtt                                                     <  1:370848/251‑1 (MQ=255)                                                                                                                                                                                             aGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTGATCAACCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCAt                                               <  1:219304/251‑1 (MQ=255)                                                                                                                                                                                               gg‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATc                                              >  2:285739/1‑250 (MQ=255)                                                                                                                                                                                                     cgcgCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCACGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGAGCGTTGAGCTGCGCCAGCTGCTTGCATCTTGCGGTTTGCAGGGTTTGCGGTATCTTCAGCAACTTAGTCTGGAAATGGTCCAGCGCGTAGTGTTTATCCTCAACCGGGCGATGCTGGGCGACTGGATCTTCACCATCAAAc                                          >  1:539676/1‑249 (MQ=255)                                                                                                                                                                                                        gcTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAgcgc                                     >  2:505666/1‑251 (MQ=255)                                                                                                                                                                                                          tCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGccc                                   >  1:480077/1‑250 (MQ=255)                                                                                                                                                                                                          ccagccagcGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCa                                   <  1:249837/250‑1 (MQ=255)                                                                                                                                                                                                              ccagcGGATGAAAACGCATCTATCACCTTGTGATCAACCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATg                                                                                                                                             <  2:315018/141‑1 (MQ=255)                                                                                                                                                                                                              ccagcGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATg                                                                                                                                             >  1:315018/1‑141 (MQ=255)                                                                                                                                                                                                               cagcGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGccc                              >  1:41915/1‑251 (MQ=255)                                                                                                                                                                                                                  cGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAAt                           >  1:575176/1‑251 (MQ=255)                                                                                                                                                                                                                   ggacgAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATg                          >  2:140081/5‑251 (MQ=255)                                                                                                                                                                                                                        aaaccGCATCTATCCCCTTGTGATCAACACCTTCATCCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCt                     <  2:261721/247‑1 (MQ=255)                                                                                                                                                                                                                                   aTCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCGTCACCATCAAACAGCGCCACGCCCAATGCCCCGGAAACgg              <  1:570119/247‑1 (MQ=255)                                                                                                                                                                                                                                        cTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCTCTGAGCTTCGCCATCAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACAcgc     >  2:580114/1‑251 (MQ=255)                                                                                                                                                                                                                                           gtgATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACAcgcgcg  <  2:375734/251‑1 (MQ=255)                                                                                                                                                                                                                                           gtgATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACAcgcgcg  >  1:243803/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     AGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCG‑ACAGGTTAACATAATTACAGCCATTTAGGG‑CCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACACGCGCG  >  NC_000913/2032185‑2032667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑