Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,365,662	G→A	S371F (TCT→TTT)	dcuA ←	C4‑dicarboxylate transporter DcuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,365,662	0	G	A	100.0%	242.2 / NA	69	S371F (TCT→TTT)	dcuA	C4‑dicarboxylate transporter DcuA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/40);  total (29/40)

GGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTT  >  NC_000913/4365419‑4365903                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   ggATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAAc                                                                                                                                                                                                                                             <  1:284955/251‑1 (MQ=255)  gATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAAc                                                                                                                                                                                                                                             >  1:55687/1‑250 (MQ=255)        aGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATGCGGTTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGTGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAAcagcggtc                                                                                                                                                                                                                                      <  1:594992/251‑1 (MQ=255)        aGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAAcagcggta                                                                                                                                                                                                                                      <  2:63405/251‑2 (MQ=255)         gTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAAcagcggtca                                                                                                                                                                                                                                     <  1:170364/251‑1 (MQ=255)                 gaaagaaaGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAACCAAACGGCCAGGGCAACACCCCGAGAACCCGGGATGAAGGACGGATGGTTGAAGACGAATTTACCGATACGGGGAGTACCCGGGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGGAGGTCGGCAGAATGAACAGACCCGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGa                                                                                                                                                                                                                              >  2:443318/1‑250 (MQ=255)                    agaaagCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACg                                                                                                                                                                                                                          <  1:179557/251‑1 (MQ=255)                         gCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTGCCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAg                                                                                                                                                                                                                     <  1:287459/251‑1 (MQ=255)                                   aaCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGAGAAACGGTCAGTGCCAGAGcc                                                                                                                                                                                                           >  2:212684/1‑251 (MQ=255)                                    aCGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCTGTCAGCGGTGAAACGTTCAGTGCCAGAGca                                                                                                                                                                                                           >  2:25971/1‑249 (MQ=255)                                                 gATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGc                                                                                                                                                                                             >  1:16922/1‑251 (MQ=255)                                                  aTCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCt                                                                                                                                                                                            >  1:341573/1‑251 (MQ=255)                                                    cATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCttt                                                                                                                                                                                          <  1:235482/251‑1 (MQ=255)                                                           gCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGCTGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCGGTGCTTTTGTGGtt                                                                                                                                                                                   >  2:43782/1‑251 (MQ=255)                                                            cATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGTGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTg                                                                                                                                                                                  <  2:240091/251‑1 (MQ=255)                                                                      cccAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTgaga                                                                                                                                                                        <  2:174319/251‑1 (MQ=255)                                                                         aGCACGAAGCCGAATCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTa                                                                                                                                                                     <  2:299780/251‑1 (MQ=255)                                                                            aCGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTAcag                                                                                                                                                                  <  1:165290/251‑1 (MQ=255)                                                                            aCGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTAcag                                                                                                                                                                  <  2:467208/251‑1 (MQ=255)                                                                             cgaagccgaagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAAca                                                                                                                                                                                                                                            <  1:310126/176‑1 (MQ=255)                                                                             cgaagccgaagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAAca                                                                                                                                                                                                                                            >  2:310126/1‑176 (MQ=255)                                                                             cgaagccgaagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTAcagc                                                                                                                                                                 <  2:579797/251‑1 (MQ=255)                                                                              gaagccgaagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTAcagc                                                                                                                                                                 >  2:212461/1‑250 (MQ=255)                                                                                     aagcAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAgcagagcaga                                                                                                                                                         <  2:333681/251‑1 (MQ=255)                                                                                         aaaCGGCCAGGGCAACACACAGAGTACCCGGGATGAAGCACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGc                                                                                                                                                      >  1:501552/1‑250 (MQ=255)                                                                                          aaCGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCGGAGCAGAAGCaa                                                                                                                                                    <  2:573344/251‑1 (MQ=255)                                                                                          aaCGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCa                                                                                                                                                     >  1:221071/1‑250 (MQ=255)                                                                                                   ggCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAagaagaaga                                                                                                                                           <  1:212461/251‑1 (MQ=255)                                                                                                   ggCAACACCCAGAGGACCCGGGATGAAGAACGGATGGATGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCCGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAaagaagn                                                                                                                                              >  2:599108/1‑247 (MQ=255)                                                                                                     cAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATg                                                                                                                                         >  1:438331/1‑251 (MQ=255)                                                                                                      aaCACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCTGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTAcagcag                                                                                                                                                               <  1:303473/228‑1 (MQ=255)                                                                                                      aaCACACAGAGAACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTAcagcag                                                                                                                                                               >  2:303473/1‑228 (MQ=255)                                                                                                           ccAGAGTACCCGGGATGAAGACCGGATGGTTGAATACGAATTTCCCGATACGGGTAGTACCCTTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTGTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAATATGACGGcc                                                                                                                                   <  2:341573/251‑1 (MQ=255)                                                                                                            cAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCca                                                                                                                                  <  2:535558/251‑1 (MQ=255)                                                                                                                       ggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACgg                                                                                                                       >  1:493568/1‑250 (MQ=255)                                                                                                                          tGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATg                                                                                                                    >  1:327751/1‑250 (MQ=255)                                                                                                                           gaagaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGGGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAg                                                                                                                                                       <  1:594364/215‑1 (MQ=255)                                                                                                                           gaagaaCGGATGGCTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTAGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTAAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGaagaag                                                                                                                                                       >  2:594364/1‑215 (MQ=255)                                                                                                                            aagaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGAcc                                                                                                                 <  1:298253/251‑1 (MQ=255)                                                                                                                                aCGGATGGATGAAGACGAATTTACCGATCCGAGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCGGAGCAGAAGCAAAGAAGAAGATGACGGCCAGACA‑ACACGGATGACCCTg                                                                                                              >  1:532937/1‑250 (MQ=255)                                                                                                                                    aTGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATc                                                                                                          >  2:108289/1‑250 (MQ=255)                                                                                                                                      ggTTGGAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACCGACCAGACACCGCAGCGAAAAACGCAACAGCGGTCAGCGGTGAAACGTTCCGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACt                                                                                                       >  2:292491/1‑251 (MQ=255)                                                                                                                                      ggTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACt                                                                                                       <  1:397763/251‑1 (MQ=255)                                                                                                                                      ggTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACt                                                                                                       <  1:383303/251‑1 (MQ=255)                                                                                                                                          gAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCa                                                                                                    <  1:338853/250‑1 (MQ=255)                                                                                                                                           aaGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCAcc                                                                                                  <  1:275749/251‑1 (MQ=255)                                                                                                                                            aGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCAcc                                                                                                  >  1:647169/1‑250 (MQ=255)                                                                                                                                                gAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCgg                                                                                             >  1:121361/1‑251 (MQ=255)                                                                                                                                                  aTTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCTTCGGGTAGGTCGGCAGAATTAACAGACCAGACACCGCTGCGAAAAAAGCAACAGCGCTCAGCGGTGAAACGTTCATTTCCAGAGCCATCGGCATCAGTCCTTTTGCTGTTGCAGCCTGAGATTACAGCAGAGCAGAAGCAAAGAAGAAGATTACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTa                                                                                           <  1:417473/251‑1 (MQ=255)                                                                                                                                                     tACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATc                                                                                         <  1:177971/250‑1 (MQ=255)                                                                                                                                                          aTACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGAt                                                                                   >  1:486401/1‑251 (MQ=255)                                                                                                                                                           tACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATc                                                                                  >  2:228834/1‑251 (MQ=255)                                                                                                                                                            aCGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATcc                                                                                 >  2:115640/1‑251 (MQ=255)                                                                                                                                                             cgtgtagtaCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCGTCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCATAGCAGAAGCAAAGAAGAAGAGGACGGCCAG‑CATCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCa                                                                                <  1:87106/248‑1 (MQ=255)                                                                                                                                                                              atccatcTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGaaa                                                               <  1:199243/251‑1 (MQ=255)                                                                                                                                                                                      gTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGaa                                                           <  1:166626/247‑1 (MQ=255)                                                                                                                                                                                            cagcaACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGccc                                                 <  2:524096/251‑1 (MQ=255)                                                                                                                                                                                               caACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGc                                              <  2:283951/251‑1 (MQ=255)                                                                                                                                                                                                  ccAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCAGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGACCCAGTCGATGTTGTTGGAACCGAAAGTATCGCCCAGCCAc                                           <  2:103869/251‑1 (MQ=255)                                                                                                                                                                                                    aGCGTCGGGTAGGGCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGGGAAACATTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGACAG‑AAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGACCAGCCACGc                                         >  1:431292/1‑251 (MQ=255)                                                                                                                                                                                                            gTAGGGCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCa                                 <  2:327751/251‑1 (MQ=255)                                                                                                                                                                                                              aGGTCGGAAGAATGAACAGACCAGATATCGCAGCGAAAAAAGCATCAGCGGGCAGCGGTGAATCGGTCAGTGCCAGAGCCATCGGCTACAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATAGCCCAGCCACGCAACACCCAgg                               <  2:532937/251‑1 (MQ=255)                                                                                                                                                                                                               ggTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAgg                               >  2:117922/1‑250 (MQ=255)                                                                                                                                                                                                                  cGGCAGAATGAACAGACCAGACACCGCAGCGAAAAAGGCAACAGCGGTCGGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTGTTGCGGTTGCAGCGTGATAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAA‑CAGCCACGGATGACCGTGAATCTCTTCACCAGCGGTATCTTTGGTCCAGTCTATGTTGTAGGAAACGAAAGTATGGccgtgccaaggaattcccaggatac                           <  2:266615/251‑24 (MQ=255)                                                                                                                                                                                                                       gtaTGAACAGACCAGAGACCGAAGCGAAAAAAGCAACAGCGGTCAGCGGAGAAACGTTCAGGGCCAGAGCCATCGGCCTCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAACCGAAAGCAGCGCCCCGCCACGCAACACCCAGGATACAAAt                       >  2:634695/3‑250 (MQ=255)                                                                                                                                                                                                                             aCAGACCAGACACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGgcg                >  1:165744/1‑251 (MQ=255)                                                                                                                                                                                                                                 cccagacACCGCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCCTCAGTGCTTTTGCGGTTGCAGCCTGAGAGTTCAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACATCACCAGCGGTATCTTTGATCCAGGCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCa            <  2:501552/250‑1 (MQ=255)                                                                                                                                                                                                                                  ccagacACCGCAGCGAAAAAAGCCACAGCGGCCAGCGGGTAAAGGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTGGCGGTTGCAGCCAGAGAGTACAGCAGAGCAGATGCAAAGAAGAAGATTACGGCCAG‑CAGCCACGGATGCCCCTGAATCACGTCCCCAGCGGTATCTTTGATGCAGTCGATGTTGTCGGAAGCGAAAGCATCGCGCAGCCACACAACACCCAGGATACAAATACAGGCGCTCAt           <  2:284873/251‑1 (MQ=255)                                                                                                                                                                                                                                           gCAGCGAAAAAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCCTCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGAACCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACCCCCAGGATACAAATACAGGCGCTCATCCCTGCttt  <  2:512994/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   GGATGTTAGTCGGCGGGAAAGAAAGCGGGGCGTGAACGCCCCGCGATTGATCATTACAGCATGAAGCTACCCAGCACGAAGCCGAAGCAAACGGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCAGTGCCAGAGCCATCGGCATCAGTGCTTTTGCGGTTGCAGCCTGAGAGTACAGCAGAGCAGAAGCAAAGAAGAAGATGACGGCCAG‑CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTT  >  NC_000913/4365419‑4365903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑