Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,524,922	G→A	A30V (GCC→GTC)	yjhH ←	putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,524,922	0	G	A	100.0%	166.2 / NA	50	A30V (GCC→GTC)	yjhH	putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/30);  total (20/30)

TTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGT  >  NC_000913/4524679‑4525150                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                     ttCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACtt                                                                                                                                                                                                                                <  1:657101/250‑1 (MQ=255)     agtagtAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGAATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCgcgc                                                                                                                                                                                                                           >  1:406992/1‑251 (MQ=255)      gtagtaGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCa                                                                                                                                                                                                                          <  2:494836/251‑1 (MQ=255)        agtagGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCAtt                                                                                                                                                                                                                        <  2:207979/251‑1 (MQ=255)        agtagGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCAtt                                                                                                                                                                                                                        <  1:420227/251‑1 (MQ=255)             gggTTGAAGGCGACGATACCATCAGCGGCGTAGGCGTGCGCATTCTGCGCCAGTTTGGCCGCATCGTCAGGGGCAGGGGAACCGACGCCAATCAATACCGGCACTCGGCCGTCGGCAAGGGTTACAGCCTCTTCGGCGAGTGCCCTGCGCCGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCtt                                                                                                                                                                                                                   <  1:104682/251‑1 (MQ=255)                               ccATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTGCGTCAGTGGAAGGGGAACCGACGCCAATCAAGACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTc                                                                                                                                                                                                 <  1:88062/251‑1 (MQ=255)                                cATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCa                                                                                                                                                                                                <  2:299299/251‑1 (MQ=255)                                     gcgcCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCTCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGTAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATg                                                                                                                                                                                           <  1:405321/251‑1 (MQ=255)                                         cGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGCACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGCACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCCTTGACTTTTTATCAAGGGTTCCGTCACGATGAAAc                                                                                                                                                                                       >  2:311322/1‑251 (MQ=255)                                          gTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACg                                                                                                                                                                                      >  1:580647/1‑251 (MQ=255)                                                 tGCGCATGCTGCGCCAGGTTGGCCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCACGGCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTACGTAACGATGAAACGTGCTgg                                                                                                                                                                                <  2:119261/250‑1 (MQ=255)                                                  gcgcATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGAt                                                                                                                                                                              <  2:419473/251‑1 (MQ=255)                                                     cATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATAcc                                                                                                                                                                           <  1:535376/251‑1 (MQ=255)                                                      aTGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCGGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGTAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGAGCTGGATACCg                                                                                                                                                                          >  1:384450/1‑251 (MQ=255)                                                           gcgcCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGa                                                                                                                                                                     <  1:135419/251‑1 (MQ=255)                                                                             cGTCAGTGGAAGGGGAACCGACTCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAAtttt                                                                                                                                                   <  1:447183/251‑1 (MQ=255)                                                                                cAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGt                                                                                                                                                                                     <  2:462171/214‑1 (MQ=255)                                                                                cAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGt                                                                                                                                                                                     >  1:462171/1‑214 (MQ=255)                                                                                    ggAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCAtt                                                                                                                                             >  2:372423/1‑250 (MQ=255)                                                                                        ggggAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAAtt                                                                                                                                                     >  2:464672/1‑238 (MQ=255)                                                                                        ggggAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAAtt                                                                                                                                                     <  1:464672/238‑1 (MQ=255)                                                                                           gAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGt                                                                                                                                     <  2:159300/251‑1 (MQ=255)                                                                                           gAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGt                                                                                                                                     >  2:408118/1‑251 (MQ=255)                                                                                                  cGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCCTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAA‑TTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATTCCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCtttt                                                                                                                             <  1:308512/251‑1 (MQ=255)                                                                                                       atcaatACCGGCACTCGCCCGTCGAAAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCTCCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTa                                                                                                                         <  2:384450/251‑1 (MQ=255)                                                                                                           atACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGGTACCGGTGGCATAATGCCGCGGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTg                                                                                                                     >  1:427211/1‑251 (MQ=255)                                                                                                                      cGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGTCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACTCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTAGCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATGACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTc                                                                                                            <  1:612568/249‑1 (MQ=255)                                                                                                                         ccGNCGACAATTGTTACAGCTTCTTCGGCGCGGGCGATGCGCTGTGCTGTATGCATGTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCTTCGACCTCTTTAGTAATCAGTAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATAAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATGACCTCAGTTTGCTTGTCGTAAGTTCTCATTCTCGTTTc                                                                                                       <  2:589385/251‑1 (MQ=255)                                                                                                                               agaaTGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATc                                                                                                 <  2:27658/249‑1 (MQ=255)                                                                                                                                 aaTGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAATCGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATccc                                                                                               <  2:547506/251‑1 (MQ=255)                                                                                                                                   tGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATccc                                                                                               >  1:151736/1‑249 (MQ=255)                                                                                                                                        aCAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCGTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCa                                                                                         <  1:546005/250‑1 (MQ=255)                                                                                                                                                  cGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTAAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATAtttc                                                                              >  1:195317/1‑250 (MQ=255)                                                                                                                                                             aTGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGCCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCAtt                                                                   >  2:570363/1‑251 (MQ=255)                                                                                                                                                                        tGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTg                                                                                                                                                                                 <  2:224192/130‑1 (MQ=255)                                                                                                                                                                        tGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTg                                                                                                                                                                                 >  1:224192/1‑130 (MQ=255)                                                                                                                                                                        tGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAAt                                                        >  2:32375/1‑251 (MQ=255)                                                                                                                                                                         gTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAg                                                                                             >  1:432409/1‑213 (MQ=255)                                                                                                                                                                         gTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAg                                                                                             <  2:432409/213‑1 (MQ=255)                                                                                                                                                                                 ttGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATa                                                <  1:298076/250‑1 (MQ=255)                                                                                                                                                                                  ttgCTAAATTCACCACCGGTCCCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAAGAAGTCGAAAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACTATGAAACGTGCTGGATACCTGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAATTGCTCATTTTTGTTTCTGTATCCCAGCCCTTATGATATTTGCTCATCTCATTTCCGATGCAATTCCATATAg                                               <  2:274206/248‑1 (MQ=255)                                                                                                                                                                                    gCTAAATTCACCACCGGTACCCAGATAAAACATCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTg                                            <  2:340712/251‑1 (MQ=255)                                                                                                                                                                                       aaaTTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCt                                         >  1:111891/1‑251 (MQ=255)                                                                                                                                                                                                accGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTAttt                                >  1:609644/1‑251 (MQ=255)                                                                                                                                                                                                     tACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTg                            >  2:535220/1‑250 (MQ=255)                                                                                                                                                                                                          aGATAAAACAGCCCGTCGACCCCTTTATTCATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATaa                       >  1:509560/1‑250 (MQ=255)                                                                                                                                                                                                                          cGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAg       <  1:349839/250‑1 (MQ=255)                                                                                                                                                                                                                              cccTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGt  >  1:393414/1‑251 (MQ=255)                                                                                                                                                                                                                              cccTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGt  <  2:13955/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                     TTCCAGTAGTAGGGGTTGATGGCGACGATACCATCAGCGCCGTAGGCTTGCGCATGCTGCGCCAGTTTGACCGCTTCGTCAGTGGAAGGGGAACCGACGCCAATCAATACCGGCACTCGCCCGTCGACAATGGTTACAGCTTCTTCGGCGAGTGCCATGCGCTGGGCTGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGT  >  NC_000913/4524679‑4525150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑