Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,529,317	C→T	L309L (CTG→CTA)	sgcC ←	putative PTS enzyme IIC component SgcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,529,317	0	C	T	100.0%	215.9 / NA	63	L309L (CTG→CTA)	sgcC	putative PTS enzyme IIC component SgcC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/32);  total (31/32)

GCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATCAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCA  >  NC_000913/4529073‑4529551                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           gcgcACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGc                                                                                                                                                                                                                                      >  2:319959/1‑251 (MQ=255)       tttCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCg                                                                                                                                                                                                                                >  1:531791/1‑251 (MQ=255)            ccGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AAAGCTGGCCAGAATTAACATAATGGGTATTAGAAGCAGACCGACAGc                                                                                                                                                                                                                           >  1:166925/1‑251 (MQ=255)             cGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGGTCAGCAGGACGGTAATCATCGCGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAGAGAACGGTGCTACCGGCAGGGCTGCGAGGGGTAACACTGTGGTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGGCCGACAGCa                                                                                                                                                                                                                          <  2:625912/251‑1 (MQ=255)             cGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AAAGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGc                                                                                                                                                                                                                           >  2:580723/1‑250 (MQ=255)                   aaCTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAAtggtg                                                                                                                                                                                                                    <  1:189733/251‑1 (MQ=255)                    aCTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGAAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAAAATAATGGGTATTAGTAGCAGAACGACAGAAAtggtgg                                                                                                                                                                                                                   >  1:107705/1‑251 (MQ=255)                            cGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGGCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCCATGGTGGTTGGATGc                                                                                                                                                                                                           >  2:136444/1‑251 (MQ=255)                                 ttGAGCGCCATTTCAGAGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGcccc                                                                                                                                                                                                        <  2:5782/249‑1 (MQ=255)                                  tGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGAACGACAGAAATGGGGGTTGGATGCCCCAGc                                                                                                                                                                                                     >  2:349525/1‑251 (MQ=255)                                   gAGCGCCATTTCAGGGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCTTAGCTATACAGATAAAGAACGGTGCTTCCGGCAGGGCTGCGAGTGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCg                                                                                                                                                                                                    <  2:415128/251‑1 (MQ=255)                                     gcgcCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTc                                                                                                                                                                                                  >  2:174683/1‑251 (MQ=255)                                       gcCATTTCAGTGAAGTAAGGCGCGAACTGGGGGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTGTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACAAAATGGGTATTAGTAGACGACCGACAGCCATGGTGGTTgggggcaccagcggcac                                                                                                                                                                                                >  2:497933/1‑236 (MQ=255)                                          aTTTCAGTGACGCAAGGAGCGAACTGTGTGGCGATCAGCAGAACGGCAGTCATAACAATGACCCCGCTTATACACGTCCTAACCCGATATCCGCGGTCGATAACCGTAGCCATACAGATAAAGAACGGTGCTACTGGCAGGTTTGCGGGGGGTCACCCTTTGCTTCCCGGTAATAAT‑CTGGCCAGAATTAAACTAATGCGTCTTAGTAACAGAACGACAGCAATGGTTGTTTGAttgcctgccgtcaccgc                                                                                                                                                                                             >  2:296015/1‑235 (MQ=255)                                                gTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATc                                                                                                                                                                                       <  2:317798/251‑1 (MQ=255)                                                       aaGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGAcc                                                                                                                                                                                 <  1:197549/250‑1 (MQ=255)                                                            gcgAACTGGGTGGCGATCAGCAGGGCGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGAtata                                                                                                                                                                           <  1:112872/251‑1 (MQ=255)                                                            gcgAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGGGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGatat                                                                                                                                                                            >  2:543287/1‑250 (MQ=255)                                                             cgAACTGGGTGGCGAGCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCACAGCGTCACAGCAGTATCCAGACCGATATac                                                                                                                                                                          <  2:230812/251‑1 (MQ=255)                                                             cgAACTGGGTGGCGACCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGAtata                                                                                                                                                                           <  2:211110/250‑1 (MQ=255)                                                              gAACTGGGAGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGACCCAGCGTGACAGCAGTATCCAGACCGATATac                                                                                                                                                                          >  1:319504/1‑250 (MQ=255)                                                                             cagcagGACGGTAATCATCACGATGACCACGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTg                                                                                                                                                           <  1:445409/250‑1 (MQ=255)                                                                                cagGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAat                                                                                                                                                       <  1:436063/251‑1 (MQ=255)                                                                                    aCGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCCGACCGATATACACTTCACGTCCTTTGAAATAttt                                                                                                                                                   <  1:426652/251‑1 (MQ=255)                                                                                     cGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTc                                                                                                                                                  <  2:661650/251‑1 (MQ=255)                                                                                         aaTCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGga                                                                                                                                              <  1:57451/251‑1 (MQ=255)                                                                                         aaTCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGACTGCGAGGGGGAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGGAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGAGTGACAGCAGTATCCAGACCGATATAAACTTCACGTCCTTTGAAATATTTCTgg                                                                                                                                               >  1:611140/1‑250 (MQ=255)                                                                                            catcaccatgtacccGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGGCAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGgaaga                                                                                                                                           <  2:612031/239‑1 (MQ=255)                                                                                            catcaCGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGgaaga                                                                                                                                           <  1:341194/251‑1 (MQ=255)                                                                                             atcaCGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTTCGAGGGGTAACACTTTGTTTCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGGCCTTTGAAATATTTCTGgaaga                                                                                                                                           >  2:448924/1‑250 (MQ=255)                                                                                               caCGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGGAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAAAGGTGGGTGGATGCCCCAGCGTCAAAGCAGGATCCAGACCGAAATACACATCACGTCCTTTGAAATATTTCTGGAAAAACt                                                                                                                                        >  1:281064/1‑251 (MQ=255)                                                                                                 cGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACttt                                                                                                                                      <  2:335833/251‑1 (MQ=255)                                                                                                 cGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGGAAAACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGGATTAGTAGCAGACCGACAGCAATTGTGGTTGGATGCCCCAGCGGCACAGCAGTATCCAGACCGATTTACACTTCCCGTCCTTTGAAATATTTCTGGAAAAACttt                                                                                                                                      >  2:556785/1‑251 (MQ=255)                                                                                                     gACCCAGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCATACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCtt                                                                                                                                   <  1:169254/250‑1 (MQ=255)                                                                                                          cGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCg                                                                                                                             >  1:23665/1‑251 (MQ=255)                                                                                                              tATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCg                                                                                                                          <  1:634877/250‑1 (MQ=255)                                                                                                               aTCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGGCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCgg                                                                                                                         >  1:450332/1‑250 (MQ=255)                                                                                                                   aCGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATg                                                                                                                    <  1:505661/251‑1 (MQ=255)                                                                                                                     gTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGGGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATggg                                                                                                                  >  1:347629/1‑251 (MQ=255)                                                                                                                       ccTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGgca                                                                                                                <  1:174683/251‑1 (MQ=255)                                                                                                                        cTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGgc                                                                                                                 >  1:444227/1‑249 (MQ=255)                                                                                                                           aCCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGcagcag                                                                                                            <  1:136444/251‑1 (MQ=255)                                                                                                                                gTCTCCGGGGTGGATAACCGTAGCCATACAGATAAAGAACGGGGCTACCGGCAGGTGTTCGGGGGGGAACAACTTGTTGGCCGGGAA‑AATGCTGGCCAGAATTAACATAAAGGGTTTTAATGGGAAACCCACAGCAATGGGGGGTGGGTGCCCCAGCGTGACCGCAGGATCCAAACCCATATAGACTTCACATCCTTTGAAATATTTTTGGAAGAACTTTCTTTCACCGGCGGAGATGGgcagccagcccc                                                                                                       >  2:576497/1‑244 (MQ=255)                                                                                                                                         gTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTTTTGCCCGGTAA‑ACTGCTCGCCAGAATTAACATAATGGGGTTTTGTAGCAGACCGACAGCCATGGGGGTTTGGTGCCCCACCGGCACAGCAGTATCCAGCCCCATATACACTTCACGTCCTTTGAAATATTTTTGGAAGAACTTTCTTGCCCCGTCGGAGATGGGCAGCAGGCCCTcaacaaac                                                                                               >  2:481514/1‑248 (MQ=255)                                                                                                                                         gTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCa                                                                                              <  1:5033/251‑1 (MQ=255)                                                                                                                                            gATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGt                                                                                                                            <  1:181102/218‑1 (MQ=255)                                                                                                                                            gATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGGCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCCGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGGATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGt                                                                                                                            >  2:181102/1‑218 (MQ=255)                                                                                                                                                  cGTAGCCATACAGATAAAGAACGGAGCTACCGGCAGGTCTGCGCGGGGAAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGGATTAGAAGCAGACCGACAGCAATGGGGGTTGGATGCCCCAGAGTCAAAGCAGGATCCAGACCGATATACACTTCAAGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGGGATGGGCAGCAGGCCCTCAACAATCAggaggagc                                                                                      >  1:380376/1‑248 (MQ=255)                                                                                                                                                        cATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGcgcg                                                                               <  1:497933/251‑1 (MQ=255)                                                                                                                                                                           gCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGGCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCTCTCAACAATCAGGCGGATCATGCGCGGGAACAACCCCATGATTg                                                             <  2:58530/250‑1 (MQ=255)                                                                                                                                                                                 ggCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGCGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGTAACAACACCATGATTGCCGCaa                                                       >  2:371771/1‑250 (MQ=255)                                                                                                                                                                                        cTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatc                                               >  1:21317/1‑251 (MQ=255)                                                                                                                                                                                            gAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcatc                                            >  1:182014/1‑250 (MQ=255)                                                                                                                                                                                              ggggTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGa                                         <  2:60965/251‑1 (MQ=255)                                                                                                                                                                                               gggTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGAc                                        <  2:348030/251‑1 (MQ=255)                                                                                                                                                                                                           gttgCCCGGTAA‑AATGCTGGTCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCGTGCACCGTCGGAGAGGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACCGTAATCATCAGACTGGCGTAACCtt                            <  2:422918/251‑1 (MQ=255)                                                                                                                                                                                                               cccGGTAA‑AATGCTGGTCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGATCGATATACACTTCACGTCCGTGGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGTGCAGCAGGCCCTCAACAAGCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCGGACTGGCGCAACCTTTaaa                        <  2:158059/251‑1 (MQ=255)                                                                                                                                                                                                                    tAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAat                                                <  1:253038/222‑1 (MQ=255)                                                                                                                                                                                                                    tAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAat                                                >  2:253038/1‑222 (MQ=255)                                                                                                                                                                                                                    tAA‑AATGCTGGCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCtt                   >  2:169851/1‑251 (MQ=255)                                                                                                                                                                                                                              ggCCAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgccg          >  2:350476/1‑251 (MQ=255)                                                                                                                                                                                                                                 cAGAATTAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgccgcc        >  1:524125/1‑250 (MQ=255)                                                                                                                                                                                                                                       tAACATAATGGGTATTAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCa  <  1:82170/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           GCGCACTTTCACCGGCAAAACTGAAACCGCCTTTGAGCGCCATTTCAGTGAAGTAAGGCGCGAACTGGGTGGCGATCAGCAGGACGGTAATCATCACGATGACCCCGCTTATCAACGTCCTGACCAGGTCTCCGCGGTGGATAACCGTAGCCATACAGATAAAGAACGGTGCTACCGGCAGGTCTGCGAGGGGTAACACTTTGTTGCCCGGTAA‑AATGCTGGCCAGAATTAACATAATGGGTATCAGTAGCAGACCGACAGCAATGGTGGTTGGATGCCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCA  >  NC_000913/4529073‑4529551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑