Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,892,044	C→T	P144S (CCA→TCA)	mdtL →	efflux pump MdtL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,892,044	0	C	T	100.0%	237.2 / NA	75	P144S (CCA→TCA)	mdtL	efflux pump MdtL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/41);  total (34/41)

GCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTC  >  NC_000913/3891798‑3892288                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     gCCGATCGTTCAGGTAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCCCGTTCTTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGt                                                                                                                                                                                                                                                  >  2:425999/1‑251 (MQ=255)            aGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATc                                                                                                                                                                                                                                       >  2:10818/1‑251 (MQ=255)            aGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATc                                                                                                                                                                                                                                       <  2:517121/251‑1 (MQ=255)             gggAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCt                                                                                                                                                                                                                                      >  2:356534/1‑251 (MQ=255)               gagaAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGa                                                                                                                                                                                                                                    <  2:499705/251‑1 (MQ=255)                agaAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGAt                                                                                                                                                                                                                                   >  1:363044/1‑251 (MQ=255)                 gaAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGAtt                                                                                                                                                                                                                                  >  1:651844/1‑251 (MQ=255)                 gaAAGCCGGTCGCCATACCCGGCGAGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCATCAGTGCTCGGACATCTGAtt                                                                                                                                                                                                                                  >  1:47405/1‑251 (MQ=255)                    aGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGTCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATg                                                                                                                                                                                                                               <  1:49978/251‑1 (MQ=255)                           cGCCATACCCGGCGCGGCGCTATTTATTATTGCCTTGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCGGGAGCATTGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGAAATCTGATTATGCTTAAAt                                                                                                                                                                                                                        >  2:573949/1‑251 (MQ=255)                           cGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAAt                                                                                                                                                                                                                        >  1:570147/1‑251 (MQ=255)                               aTACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTccc                                                                                                                                                                                                                    <  1:25838/251‑1 (MQ=255)                                  cccGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCTCTGGCTGAAACCAGCACGTTATTCCGTGCAGGCCGATTTCTACAGGTGTTGGTCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCCACACACTGGATGATCGGTGTCGGGCTTAAGTGCTGTCTTTACTCAACGGTATTTCCTGCATCATTCCCGTGTTCGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTg                                                                                                                                                                                                                 <  2:520862/251‑1 (MQ=255)                                      gcgcggcgcTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGTCGATTTCTACAGGGGTTGGACGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTCAAGTGCTGTCATTACTCATCGGTAGTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCag                                                                                                                                                                                                             <  1:438918/251‑1 (MQ=255)                                       cgcngcgcTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGAGCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCaga                                                                                                                                                                                                            <  2:599840/251‑1 (MQ=255)                                         cggcgcTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGt                                                                                                                                                                                                          >  1:3685/1‑251 (MQ=255)                                           gcgcTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGGTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGGGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGt                                                                                                                                                                                                          <  2:22/249‑1 (MQ=255)                                                atttattATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCGGAGTCTGTTCt                                                                                                                                                                                                   <  1:129482/251‑1 (MQ=255)                                                   tattatTGCCGCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTgg                                                                                                                                                                                                 <  1:374595/250‑1 (MQ=255)                                                          gCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATgg                                                                                                                                                                                          <  2:464403/250‑1 (MQ=255)                                                                  gttctgttcACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATgg                                                                                                                                                                                 <  1:10818/251‑1 (MQ=255)                                                                       gttcACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCAt                                                                                                                                                                             <  1:502400/250‑1 (MQ=255)                                                                         tcACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATcg                                                                                                                                                                           <  2:327049/250‑1 (MQ=255)                                                                              ggCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTTCCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTAc                                                                                                                                                                     <  2:3685/251‑1 (MQ=255)                                                                               gCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCATGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACt                                                                                                                                                                    <  2:614800/251‑1 (MQ=255)                                                                                 tGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTg                                                                                                                                                                   >  1:463771/1‑250 (MQ=255)                                                                                  gAAACCAGCACGTTATTTCTTGCAGTCCGATTTCTACAGGGGTTGGGCTCAGGCTGTTGTTACGGAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGTGGTACTg                                                                                                                                                                   <  2:347653/249‑1 (MQ=255)                                                                                  gAAACCAGAACGTTATTGCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGa                                                                                                                                                                  <  2:561645/250‑1 (MQ=255)                                                                                      ccAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGAtgttg                                                                                                                                                             <  1:158731/251‑1 (MQ=255)                                                                                      ccAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGGAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGAtgttg                                                                                                                                                             >  1:21362/1‑251 (MQ=255)                                                                                        aGCACGTTATTTCTTGAAGGACGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTc                                                                                                                                                           >  2:52950/1‑251 (MQ=255)                                                                                                aTTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCAt                                                                                                                                                                             <  1:204673/225‑1 (MQ=255)                                                                                                aTTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCAt                                                                                                                                                                             >  2:204673/1‑225 (MQ=255)                                                                                                      ttcAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCTTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTAGCTGCATCATTCCGGTGTTAGCGTCAGTGCTCTGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCGGTTCTGGGGGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTa                                                                                                                                              <  1:596936/248‑1 (MQ=255)                                                                                                      tGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTAtttt                                                                                                                                               <  1:356534/249‑1 (MQ=255)                                                                                                         aGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGCTCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTaaa                                                                                                                                            >  1:630700/1‑249 (MQ=255)                                                                                                          ggCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGa                                                                                                                                         <  2:225472/251‑1 (MQ=255)                                                                                                              gATTTCTACAGGGGTTGGGCGCAGGGTGTTGTTACGTAGTGGCGTTCGCTATTTTGCTCGCCACGCTGGAAGATCGTCGTCGGGCGAAAGTGCTGTCATTACTCAACGGGTTTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGTCAGAGTCGGTTCTTGGCGATGGCAATGATGGGCCTCGCGGTACTGATGTTTTCGTTGTTTAGTGTAAAAGAAAcg                                                                                                                                     <  2:132788/251‑1 (MQ=255)                                                                                                                 ttCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAAcgcg                                                                                                                                   <  2:248756/250‑1 (MQ=255)                                                                                                                  tCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGcc                                                                                                                                 >  1:368664/1‑251 (MQ=255)                                                                                                                     aCAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGccc                                                                                                                                >  1:659203/1‑249 (MQ=255)                                                                                                                       aGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCg                                                                                                                            <  1:533608/251‑1 (MQ=255)                                                                                                                           gTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGccc                                                                                                                        <  1:281771/251‑1 (MQ=255)                                                                                                                                gcgcAGGCTGTTGTTACGTAGGGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGTCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCa                                                                                                                   <  1:192169/251‑1 (MQ=255)                                                                                                                                    aGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCtt                                                                                                               >  1:394146/1‑251 (MQ=255)                                                                                                                                       cTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCgg                                                                                                            >  1:509312/1‑251 (MQ=255)                                                                                                                                       cTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGCGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTCTTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTCCgg                                                                                                            >  1:592079/1‑251 (MQ=255)                                                                                                                                        tgttgtTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCgg                                                                                                            >  1:471707/1‑250 (MQ=255)                                                                                                                                        tgttgtTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCgg                                                                                                            >  2:43401/1‑250 (MQ=255)                                                                                                                                                            cGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGAGTCAGTGCTCGGACATCTGATTGTGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCg                                                                                       >  2:33186/1‑251 (MQ=255)                                                                                                                                                             gCTATTTTGCGCGACACGCTGGATGGTCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAAGGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCGTCGGATAAACCACGAGAAAAGAGCGa                                                                                      <  2:630700/251‑1 (MQ=255)                                                                                                                                                                       gcgACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCtt                                                                            <  2:659203/251‑1 (MQ=255)                                                                                                                                                                                tGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGnttt                                                                   >  2:590982/1‑251 (MQ=255)                                                                                                                                                                                 ggATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGttt                                                                    <  2:463771/249‑1 (MQ=255)                                                                                                                                                                                     gatCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTAAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCt                                                              <  1:660218/251‑1 (MQ=255)                                                                                                                                                                                      atCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTc                                                             <  2:471707/251‑1 (MQ=255)                                                                                                                                                                                        cGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTGTTTTCCTCAg                                                           <  2:81937/251‑1 (MQ=255)                                                                                                                                                                                         gACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGAAGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCTCTGCTTAACCGTTTTTTCCTCAGc                                                          >  2:453286/1‑251 (MQ=255)                                                                                                                                                                                               gggCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATCATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGtgtt                                                    >  1:18141/1‑251 (MQ=255)                                                                                                                                                                                                         gCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATcacc                                           >  1:555242/1‑250 (MQ=255)                                                                                                                                                                                                          cTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATcaccac                                         >  2:804/1‑251 (MQ=255)                                                                                                                                                                                                            gTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCGCAGCCGTGTTGTTATCACCAccc                                       <  2:59906/251‑1 (MQ=255)                                                                                                                                                                                                                  aCTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCGTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGc                                  <  1:220981/250‑1 (MQ=255)                                                                                                                                                                                                                    tCAACGGTATTACCTGCATAATTCCGGGGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTATTTGTTTATTTTAAAAGAAAAGCGCCCAGCGGTCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGCGTTGTTATCACCACCCTCAGcttt                               >  2:12769/1‑248 (MQ=255)                                                                                                                                                                                                                      aaCGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGGACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAATCCACGAGAAAATAGCGAGTCGCTGCTTAACCGTGTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTc                             <  1:629905/251‑1 (MQ=255)                                                                                                                                                                                                                       aCGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAAcc                                                                                                     >  1:542087/1‑178 (MQ=255)                                                                                                                                                                                                                       aCGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAAcc                                                                                                     <  2:542087/178‑1 (MQ=255)                                                                                                                                                                                                                        cGGTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCgg                           >  1:107423/1‑251 (MQ=255)                                                                                                                                                                                                                         ggTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGt                          <  2:286150/251‑1 (MQ=255)                                                                                                                                                                                                                          gTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTg                         >  2:44358/1‑251 (MQ=255)                                                                                                                                                                                                                          gTATTACCTGCATCATTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTAATTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTg                         >  2:401438/1‑251 (MQ=255)                                                                                                                                                                                                                                    catcatTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAAt                                                                                           <  2:546813/175‑1 (MQ=255)                                                                                                                                                                                                                                    catcatTCCGGTGTTAGCGTCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAAt                                                                                           >  1:546813/1‑175 (MQ=255)                                                                                                                                                                                                                                              gtgtTAGCGTCAGTACTCGGACATCTGATTATGCTTAAATTCACGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAacac     >  1:525387/1‑251 (MQ=255)                                                                                                                                                                                                                                                  tAGCGTCAGTGCTCGGTCATCTGAGTATGCGTAAATTCCCGTGGCAGATTCTGTTCTGGGCGATGGCAATGATGGGCATAGCGGTACTGATGTTGTCTTTGTTTATTGTAAAAGAAACGGGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCATCAGCCGTGTTGGTATCACGACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTc  <  2:623581/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     GCCGATCGTTCAGGGAGAAAGCCGGTCGCCATACCCGGCGCGGCGCTATTTATTATTGCCTCGGTGTTCTGTTCACTGGCTGAAACCAGCACGTTATTTCTTGCAGGCCGATTTCTACAGGGGTTGGGCGCAGGCTGTTGTTACGTAGTGGCGTTCGCTATTTTGCGCGACACGCTGGATGATCGACGTCGGGCTAAAGTGCTGTCATTACTCAACGGTATTACCTGCATCATTCCGGTGTTAGCGCCAGTGCTCGGACATCTGATTATGCTTAAATTCCCGTGGCAGAGTCTGTTCTGGGCGATGGCAATGATGGGCATCGCGGTACTGATGTTGTCTTTGTTTATTTTAAAAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTC  >  NC_000913/3891798‑3892288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑