Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	921,756	C→T	N470N (AAC→AAT)	macB →	ABC‑type tripartite efflux pump ATP binding/membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	921,756	0	C	T	100.0%	151.8 / NA	47	N470N (AAC→AAT)	macB	ABC‑type tripartite efflux pump ATP binding/membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/22);  total (25/22)

TTAATCAGGAGCAGCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGAC  >  NC_000913/921515‑921992                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             ttAATCAGGCGCAGCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGGCAGTAATACCCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGGCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATAACGGTGCGAGTTATGGGCCATTCTTGGCTTAATTCCATTa                                                                                                                                                                                                                                       <  2:140531/249‑1 (MQ=255)    aTCAGGAGCAGCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTc                                                                                                                                                                                                                                  >  2:263101/1‑251 (MQ=255)      cAGGAGCAGCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTc                                                                                                                                                                                                                                  >  1:51467/1‑249 (MQ=255)           gcagcTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTTCCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGa                                                                                                                                                                                                                           <  2:51467/251‑1 (MQ=255)                     ggTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGAtt                                                                                                                                                                                                                 <  2:155665/251‑1 (MQ=255)                              cAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGAtt                                                                                                                                                                                                                 >  1:429222/1‑242 (MQ=255)                              cAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGAtt                                                                                                                                                                                                                 <  2:429222/242‑1 (MQ=255)                                ggTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCg                                                                                                                                                                                                      <  2:430175/251‑1 (MQ=255)                                    gTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGGGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGGGAAAGAAGGATTTGACAGCGCCGAGGc                                                                                                                                                                                                  >  2:583169/1‑251 (MQ=255)                                              aCAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGCGGCGGAACAGcc                                                                                                                                                                                         >  2:169176/1‑249 (MQ=255)                                                      aCTCGCCGCCAGCTTTTCCCCCATAAGGCAGATGTGGTTGGCGCGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGCGTGGCGGAAGACAAACCGTCGATGTTTGGTAGCAGTAAAGTGCTGCGGGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGCGCCTTAATTCCAATACTGTTAGGGTGAAAGAAGGATTTGACAGCGCCGAGGAGGAACAGCAACTCAcg                                                                                                                                                                                  >  2:114932/1‑249 (MQ=255)                                                      aCTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCAcgcg                                                                                                                                                                                >  1:606709/1‑251 (MQ=255)                                                        tCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGtt                                                                                                                                                                              >  1:60825/1‑251 (MQ=255)                                                         cgccgcCCGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGttt                                                                                                                                                                             <  2:572857/251‑1 (MQ=255)                                                         cgccgcCAGCTTTTCCCCCATAAAGCCGTTGTGTTTGGCGAGTTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAATAAAAACAGTCTATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCTAGTTATGTGCCAGTCGTGGCTTAATTCCAGTTCTGTCAGGGGTAATGATGGATTTGACAGCGCCGAGGGGGAATACCAACTCACGCGt                                                                                                                                                                               <  2:398507/249‑1 (MQ=255)                                                                                        tgGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGGAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGCTTCCTTTCACTGCGCCACGGACAGAAGGAtttt                                                                                                                                              >  2:559954/1‑250 (MQ=255)                                                                                               cGAGGTGATTCTGGTCGGCAATATGCCCTCCAGATTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACATCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCt                                                                                                                                       <  1:611049/251‑1 (MQ=255)                                                                                                 aGGTGATTGTGGTCGGCAATAAGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAATAGTAGATGATTGGCAGCAGTAAAGCGCAGCGTGTCAGGCAACCTTACAGCACGATGTCAGGGCGAGTTATGGGCCAGTCGAGGCTTAATTCCATTCCTGTCAGGGCGAAAGACGGAGTTGACAGCGCCGAGGCGGAACAGCAACTCCCGCGTTTAATTTCACTGCGCACCGGAAGGAAGGATTTCTTTACCTgg                                                                                                                                     >  2:618822/1‑251 (MQ=255)                                                                                                   gttaTTCTGGTCGGAAATATTCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGaa                                                                                                                                   <  2:292259/248‑1 (MQ=255)                                                                                                      aTTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCAGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGACGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTATTTACCTGGAACAt                                                                                                                                >  2:441786/1‑251 (MQ=255)                                                                                                          tGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGAtt                                                                                                                                                >  2:528652/1‑231 (MQ=255)                                                                                                          tGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGAtt                                                                                                                                                <  1:528652/231‑1 (MQ=255)                                                                                                             tCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGGCGATGTTTGGTAGCAGTAAAGTGCTGCGGGTCTGGCTACCTTACAGCAAGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGGGAAAGAAGGATTTGAAAGAGCCGAGGCGGGACAGCAACTCACGCGTTTAATTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACggg                                                                                                                         >  2:242640/1‑250 (MQ=255)                                                                                                              cGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGCAAAACAGTCGCTGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCg                                                                                                                        >  1:259149/1‑251 (MQ=255)                                                                                                                     atGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAAAAGTCGATGTTTGGTAGCAGTAAAGAGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGa                                                                                                                  >  1:121060/1‑250 (MQ=255)                                                                                                                                    aTTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTAATTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGAc                                                                                                  >  1:588031/1‑251 (MQ=255)                                                                                                                                            ggCGGAAGAAAAACAGTTGATGGTTGGTAGGAGTAAAGTGCTTGGTGTCGGGGTACCTTTCAGCACGATGTCCGGGCGAGTTGTGGGCCAGTCGTTGCTTAATTCCAATACTGTCAGGGTGGAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGt                                                                                           <  2:446256/250‑1 (MQ=255)                                                                                                                                                  agaaAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACTATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTGACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTAc                                                                                    <  1:265982/251‑1 (MQ=255)                                                                                                                                                     caaaCAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAAc                                                                                 >  2:366385/2‑251 (MQ=255)                                                                                                                                                          aGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATGACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAATACCACACGTACTTTACAACTGttt                                                                            <  2:355719/251‑1 (MQ=255)                                                                                                                                                           gTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTc                                                                           >  2:9667/1‑251 (MQ=255)                                                                                                                                                                      gTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTAc                                                                                                                                         <  1:164264/178‑1 (MQ=255)                                                                                                                                                                      gTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTAc                                                                                                                                         >  2:164264/1‑178 (MQ=255)                                                                                                                                                                               aaGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGAtt                                                       <  1:263101/251‑1 (MQ=255)                                                                                                                                                                                aGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGAttt                                                      >  2:371470/1‑251 (MQ=255)                                                                                                                                                                                     tgcGTGTCAGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTAATTTCACTGAGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTACTGACGCTGGTGGCGGTGATTTCGCtg                                                 >  1:166823/1‑251 (MQ=255)                                                                                                                                                                                                 tACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGt                                     >  1:127977/1‑251 (MQ=255)                                                                                                                                                                                                  cccTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGTTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCCCGGAAAGAAGGATTTCTTTCCCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGCCGGTa                                    <  2:563431/250‑1 (MQ=255)                                                                                                                                                                                                  aCCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTa                                    <  1:641071/251‑1 (MQ=255)                                                                                                                                                                                                          gCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTc                                                                                                                      <  1:162955/161‑1 (MQ=255)                                                                                                                                                                                                          gCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTc                                                                                                                      >  2:162955/1‑161 (MQ=255)                                                                                                                                                                                                           cACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACtt                                                                                       <  2:112960/191‑1 (MQ=255)                                                                                                                                                                                                           cACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACtt                                                                                       >  1:112960/1‑191 (MQ=255)                                                                                                                                                                                                             cGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTCCCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGTGCGGTATTGGTGTAATg                         <  1:354735/251‑1 (MQ=255)                                                                                                                                                                                                                tGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGGGGTGGGCGGTATTGGTGgaatgaat                      >  2:67233/1‑251 (MQ=255)                                                                                                                                                                                                                   ccGGGCGAGTTATGGGCCAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAatat                    <  1:169176/250‑1 (MQ=255)                                                                                                                                                                                                                                    cAGTCGTGGCTTAATTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGAc  <  1:371470/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             TTAATCAGGAGCAGCTGAACGGTCGTGCGCAGGTCGTGGTTCTCGACAGTAATACTCGCCGCCAGCTTTTCCCCCATAAAGCAGATGTGGTTGGCGAGGTGATTCTGGTCGGCAATATGCCCGCCAGAGTCATTGGTGTGGCGGAAGAAAAACAGTCGATGTTTGGTAGCAGTAAAGTGCTGCGTGTCTGGCTACCTTACAGCACGATGTCCGGGCGAGTTATGGGCCAGTCGTGGCTTAACTCCATTACTGTCAGGGTGAAAGAAGGATTTGACAGCGCCGAGGCGGAACAGCAACTCACGCGTTTACTTTCACTGCGCCACGGAAAGAAGGATTTCTTTACCTGGAACATGGACGGCGTCTTGAAAACTGTTGAAAAGACCACACGTACTTTACAACTGTTTCTGACGCTGGTGGCGGTGATTTCGCTGGTGGTGGGCGGTATTGGTGTAATGAATATTATGCTGGTGTCAGTGAC  >  NC_000913/921515‑921992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑