Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	812,699	C→T	L144L (CTG→TTG)	bioD →	dethiobiotin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	812,699	0	C	T	97.4%	248.5 / NA	76	L144L (CTG→TTG)	bioD	dethiobiotin synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (41/33);  minor base G (0/2);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACAGCAGCCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTT  >  NC_000913/812452‑812943                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     cAACAGCAGCCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACGCTGGCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTgg                                                                                                                                                                                                                                                   <  1:58220/251‑1 (MQ=255) cAACAGCAGCCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTgg                                                                                                                                                                                                                                                   <  1:446525/251‑1 (MQ=255) cAACAGCAGCCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTgg                                                                                                                                                                                                                                                   >  2:379233/1‑251 (MQ=255)   aCAGCAGCCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGCGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGt                                                                                                                                                                                                                                                  >  1:38857/1‑250 (MQ=255)          ccTGCAGCTGGATTACGCACCAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGgtg                                                                                                                                                                                                                                          >  1:543859/1‑251 (MQ=255)             gCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGtgtg                                                                                                                                                                                                                                        >  1:406619/1‑250 (MQ=255)               aGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCCGATTGGGTAACACAGGAACA‑ACTGCCGGGGATATTGGTAGTTGGGGTGaa                                                                                                                                                                                                                                      >  2:458154/1‑250 (MQ=255)               aGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATCTTGGTAGTTGGTGTGaa                                                                                                                                                                                                                                      >  2:92976/1‑250 (MQ=255)                    gATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCgg                                                                                                                                                                                                                                >  1:521473/1‑251 (MQ=255)                     aTTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGc                                                                                                                                                                                                                               <  1:56562/251‑1 (MQ=255)                         cGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTa                                                                                                                                                                                                                           <  1:559765/251‑1 (MQ=255)                           cAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTAtt                                                                                                                                                                                                                         >  2:93814/1‑251 (MQ=255)                                  aaaTCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCAcg                                                                                                                                                                                                                  <  2:556046/251‑1 (MQ=255)                                          acacCTTCGCAGAACCCACATCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGCATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑AATGCCGGTGATATTGGTAGTTGGTGTGAAACTAGGCTGTATTAATCACGCGATGTTg                                                                                                                                                                                                          >  2:48760/1‑251 (MQ=255)                                               ttCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGc                                                                                                                                                                                                     >  1:449083/1‑251 (MQ=255)                                                tCGCAGATCCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGATACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGc                                                                                                                                                                                                     <  2:121844/250‑1 (MQ=255)                                                 cGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGcac                                                                                                                                                                                                   <  2:449083/251‑1 (MQ=255)                                                 cGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGcac                                                                                                                                                                                                   >  1:503683/1‑251 (MQ=255)                                                     gAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAggg                                                                                                                                                                                               >  1:258779/1‑250 (MQ=255)                                                               cgccgcACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAacac                                                                                                                                                                                     <  2:524398/251‑1 (MQ=255)                                                                  cgcACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTAAGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACAAGcc                                                                                                                                                                                  >  2:187775/1‑251 (MQ=255)                                                                   gcACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGCTATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCg                                                                                                                                                                                 >  1:294954/1‑251 (MQ=255)                                                                       atcatcAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGgact                                                                                                                                                                             >  1:596383/1‑251 (MQ=255)                                                                         catcaGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGgactg                                                                                                                                                                            <  1:248076/250‑1 (MQ=255)                                                                             aGCGTGCAAGAGGGCATACCGATAGAATCTGTGGTAATTAGCGCCGGATTACGCGCGCTTGAACAACAGACTGACTGGTTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGTCGCGTGTTTACTCTTTCACTTTTGCAGATTTTGTAACACAGTATCACAC‑GCCGGTGATATTGTTAGTTGGTTTGAAACTCGGCTGTATTAATGACGCGATGTTCACTGCACTGGttattaaaatcgtaggactgacgct                                                                                                                                                                       <  2:396914/251‑25 (MQ=255)                                                                                gcgcAAGAGGGCAGACCGATAGAATCATTGGGAATGAGAGCCGGATTACGCGCGCTTGACAAACAGGCTGACTGGGTGTTAGTGGAAGGTGCGGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACGCAGGAACA‑CCTGCCGGTGATAGTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACGGACGCTGGc                                                                                                                                                                    <  2:303716/251‑1 (MQ=255)                                                                                  gcAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCgg                                                                                                                                                                  <  2:40736/251‑1 (MQ=255)                                                                                  gcAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGAGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCgg                                                                                                                                                                  >  2:37568/1‑251 (MQ=255)                                                                                     agagGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGGGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCgggat                                                                                                                                                               >  1:299133/1‑249 (MQ=255)                                                                                       agGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTgg                                                                                                                                                             <  2:556258/251‑1 (MQ=255)                                                                                          gCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGGGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGggtgggtg                                                                                                                                                          >  1:363413/1‑251 (MQ=255)                                                                                           cAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTgg                                                                                                                                                         <  2:482874/251‑1 (MQ=255)                                                                                            aGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGTCGGCTGGTTTACGCCGCTTTCTGACACTTTCTCTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGTCGGGTTGGGTGGc                                                                                                                                                        <  2:252025/251‑1 (MQ=255)                                                                                            aGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGc                                                                                                                                                        >  1:268854/1‑251 (MQ=255)                                                                                              aCCGATAGAATCATTGGTAATGAGCGCCGTATTACGAGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGCCACTTTCACTTTTGCGGAGTGGGTAACACCGGAACA‑CCGGCAGGGGATTTTGGTAGTTGGTGTGAAACTCGGCTGTCTTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGGTGGGTGGCgg                                                                                                                                                      >  2:61962/1‑250 (MQ=255)                                                                                               ccGATAGAATCATTGGTAATTAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTGCGCCGTTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGACCA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTGGACTGCACATGTAATACAACACGCCGGACTGACTCTGGTGGGTTGGGTGGCGaa                                                                                                                                                     <  2:354111/251‑1 (MQ=255)                                                                                                    aGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATg                                                                                                                                                <  1:320741/251‑1 (MQ=255)                                                                                                      aaTCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGtt                                                                                                                                              <  1:350260/251‑1 (MQ=255)                                                                                                                          ggATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCa                                                                                                                          <  1:248750/251‑1 (MQ=255)                                                                                                                          ggATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGGTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGGTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTc                                                                                                                           >  1:303219/1‑250 (MQ=255)                                                                                                                                  cgcgcTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGGAATACAACACGCCGGACTGACTCTGGCGGGGTGGGTGGCGAACGATGGTACGCCCCCGGGAAAACGGCACGCTggat                                                                                                                  >  2:564701/1‑248 (MQ=255)                                                                                                                                      cTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAatatat                                                                                                              >  1:144343/1‑251 (MQ=255)                                                                                                                                        tGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAatatat                                                                                                              <  1:269291/249‑1 (MQ=255)                                                                                                                                                    tGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCAccc                                                                                                >  2:94569/1‑251 (MQ=255)                                                                                                                                                    tGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGATTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCAccc                                                                                                >  1:559655/1‑251 (MQ=255)                                                                                                                                                      aCTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGc                                                                                              >  2:568068/1‑251 (MQ=255)                                                                                                                                                         gggTGTTAGTGGAAGTTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGTTGATATTGGTAGTTGGTGTGAAACTCGGCTTTATTAATCACGCGATGTTGCCTGCACAGGTAATACAACACGCCGGACTGACGCTGGCGGGTTGGGTGGCGAACGATGTTACGCGTCCGGGATAACGTCACGCTGAATATATGACCACGCTCACCCGCATg                                                                                           <  1:71981/251‑1 (MQ=255)                                                                                                                                                             gtTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTc                                                                                       <  2:543859/251‑1 (MQ=255)                                                                                                                                                                aGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCcg                                                                                    <  2:234145/251‑1 (MQ=255)                                                                                                                                                                             ggcggcTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTgg                                                                        <  1:41735/250‑1 (MQ=255)                                                                                                                                                                              gcggcTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGGCCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGga                                                                      <  2:406619/251‑1 (MQ=255)                                                                                                                                                                                ggcTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTggg                                                                       >  1:189755/1‑248 (MQ=255)                                                                                                                                                                                   tGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGCACTGACTCTGGCGGGTTGGGTGGCGAAGGATTTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCCCACCCGCATGATGCCCGCCCCGCCGCTggaag                                                                     >  2:237710/1‑244 (MQ=255)                                                                                                                                                                                   tGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATAGTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGgagaga                                                                  <  1:220076/250‑1 (MQ=255)                                                                                                                                                                                    ggTTTACGCCGCATTCTGACACTTTCACTTGTGCAGATTGGGTAACACAGGAACA‑ACTTCCGGTGATATTGGTAGTTGGTGGGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGGCACGCTGAATAGATGACCACGCTCACCCGCATGATTCCCGCGACGCTGCTGGGAGAGAt                                                                 <  2:237417/250‑1 (MQ=255)                                                                                                                                                                                               cTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGc                                                     >  1:450523/1‑251 (MQ=255)                                                                                                                                                                                                tttCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAGCACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGc                                                     >  2:2426/1‑250 (MQ=255)                                                                                                                                                                                                  tCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAg                                                   <  2:194396/250‑1 (MQ=255)                                                                                                                                                                                                   cTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGa                                                  >  1:5309/1‑250 (MQ=255)                                                                                                                                                                                                     gACACTTTCAATTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGaaa                                                >  1:451702/1‑250 (MQ=255)                                                                                                                                                                                                       cactttcactttTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATc                                             <  2:299573/251‑1 (MQ=255)                                                                                                                                                                                                       cactttcactgtTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATc                                             <  1:439962/251‑1 (MQ=255)                                                                                                                                                                                                          tttcactttTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGCTTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATcc                                            >  2:203853/1‑249 (MQ=255)                                                                                                                                                                                                            tcactttTGCAGATTGGGTAACACAGGAACA‑ACTGACG‑TGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACCGGTAATACAACACGCCGGACTGACTCTGGCGGGGTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGATCACCCGCATGATTCCCGCGCCGCTCCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGaa                                        >  2:458513/1‑250 (MQ=255)                                                                                                                                                                                                              actttTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGaaa                                       >  1:144648/1‑250 (MQ=255)                                                                                                                                                                                                                 tttGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATTATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAAt                                     <  1:187775/249‑1 (MQ=255)                                                                                                                                                                                                                     cAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCCGAAAATCCAGAAAATGCGGc                                >  1:269346/1‑250 (MQ=255)                                                                                                                                                                                                                        aTTGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCCAcc                            >  2:181162/1‑251 (MQ=255)                                                                                                                                                                                                                         ttGGGTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCg                           >  2:612054/1‑251 (MQ=255)                                                                                                                                                                                                                           gggTAACACAGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCgg                          <  2:503683/250‑1 (MQ=255)                                                                                                                                                                                                                                 cacaGGAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTAc                   >  1:254966/1‑251 (MQ=255)                                                                                                                                                                                                                                      gAACA‑ACTGCCGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATaaa              <  1:92976/251‑1 (MQ=255)                                                                                                                                                                                                                                                ccGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTc                                                                                                                           >  2:46887/1‑133 (MQ=255)                                                                                                                                                                                                                                                ccGGTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTc                                                                                                                           <  1:46887/133‑1 (MQ=255)                                                                                                                                                                                                                                                  ggTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATGTGAACACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCttgt   >  1:653219/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gTGATATTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACGGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCttgtt  <  1:317607/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     CAACAGCAGCCTGCAGCTGGATTACGCAACAGTAAATCCTTACACCTTCGCAGAACCCACTTCGCCGCACATCATCAGCGCGCAAGAGGGCAGACCGATAGAATCATTGGTAATGAGCGCCGGATTACGCGCGCTTGAACAACAGGCTGACTGGGTGTTAGTGGAAGGTGCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACA‑ACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTT  >  NC_000913/812452‑812943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑