Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,098,239	C→T	R118R (CGC→CGT)	ghrA →	glyoxylate/hydroxypyruvate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,098,239	0	C	T	100.0%	156.2 / NA	46	R118R (CGC→CGT)	ghrA	glyoxylate/hydroxypyruvate reductase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/26);  total (20/26)

CTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTA  >  NC_000913/1098001‑1098479                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 ctgATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAAt                                                                                                                                                                                                                                      >  1:298029/1‑251 (MQ=255)            ttAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCAtt                                                                                                                                                                                                                            <  1:20354/250‑1 (MQ=255)              aGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGc                                                                                                                                                                                                                         >  1:420603/1‑251 (MQ=255)                      aTCCTCCTGTTGAAATGCTGGCAGGGCTCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGCTTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGCAGATACCGGTATGTGCGAGCACATGCTGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGCCGTTTTTACGATTATCTTATCCCGCAAAATAGTTCGCATTGGCAACCGCtc                                                                                                                                                                                                                 >  2:133251/1‑250 (MQ=255)                           cctGTTGAAATGCTGGCAGGGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGGCGATTATCGTATCCAGCAAAATAGTTCGCAGTGGCAACCGCTGCCTGa                                                                                                                                                                                                            <  1:551029/251‑1 (MQ=255)                                   aaTGCTGGCAGGGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATatcatc                                                                                                                                                                                                    <  2:635541/251‑1 (MQ=255)                                         ggCAGGGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAg                                                                                                                                                                                              <  2:298029/251‑1 (MQ=255)                                             gggCGCGATCTTAAAGCGGTGTTCGCACGCGGGTCCGGTGTTGATTCTATTTTGTGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGTTGCTGCATTGGTTTCGACTTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGTGAAGAttt                                                                                                                                                                                          <  2:626886/251‑1 (MQ=255)                                                  cgATCTTAACGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTAcc                                                                                                                                                                                      >  2:645080/1‑250 (MQ=255)                                                   gATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCAt                                                                                                                                                                                    >  2:98535/1‑251 (MQ=255)                                                        tAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGc                                                                                                                                                                                <  1:304560/250‑1 (MQ=255)                                                               gtgtTAGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAGATGCTGAACCCTGCTGTTCGACTTTTTCGCCTGGAAGATACCAGTATAGTCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTggg                                                                                                                                                                        <  2:547231/251‑1 (MQ=255)                                                                tgtTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGgc                                                                                                                                                                       >  2:6030/1‑251 (MQ=255)                                                                            gggCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTg                                                                                                                                                           >  1:572283/1‑251 (MQ=255)                                                                             ggCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATc                                                                                                                                                                                   >  2:453930/1‑226 (MQ=255)                                                                             ggCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATc                                                                                                                                                                                   <  1:453930/226‑1 (MQ=255)                                                                                cGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCa                                                                                                                                                       <  1:10115/251‑1 (MQ=255)                                                                                             aTTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTc                                                                                                                                           >  1:631657/1‑250 (MQ=255)                                                                                                           aCAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCGCAGAGTCTGCAAACCt                                                                                                                            <  2:523332/251‑1 (MQ=255)                                                                                                            cAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTg                                                                                                                           >  2:274249/1‑251 (MQ=255)                                                                                                             aGGCACACGGTGAAATGCTGACCCATTCTGTTCCACTCTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGAAGATCATCGTATCCAGCAACATAGTTCGCATTGGCACCCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCCGTACAGTTGCTCAGAGTCTGCAAACCTgg                                                                                                                          >  2:317455/1‑251 (MQ=255)                                                                                                                 acacCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCt                                                                                                                      >  1:181701/1‑251 (MQ=255)                                                                                                                     ccTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTcc                                                                                                                  <  1:365959/251‑1 (MQ=255)                                                                                                                           aTGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATAACGGTATGGGCGAGCAAATGAAGGAATATGCTGTCAGTCAGGCGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTATGCAAACCTGGCGCTTTCCGCTGCg                                                                                                            >  2:380968/1‑251 (MQ=255)                                                                                                                                 aaCCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTg                                                                                                      <  2:203667/251‑1 (MQ=255)                                                                                                                                  aCCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTgg                                                                                                     <  1:337589/251‑1 (MQ=255)                                                                                                                                    ccTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAg                                                                                                   <  1:283964/251‑1 (MQ=255)                                                                                                                                       tCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCg                                                                                                >  1:381359/1‑251 (MQ=255)                                                                                                                                            tCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAAcc                                                                                            <  1:274249/250‑1 (MQ=255)                                                                                                                                             ccACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCg                                                                                          <  1:315642/251‑1 (MQ=255)                                                                                                                                                cTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTa                                                                                        >  1:110727/1‑250 (MQ=255)                                                                                                                                                           ggAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTg                                                                            <  1:645080/251‑1 (MQ=255)                                                                                                                                                                        aTGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAGTATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCtt                                                               >  2:223377/1‑251 (MQ=255)                                                                                                                                                                                gCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGAc                                                       <  2:110727/251‑1 (MQ=255)                                                                                                                                                                                      gCAGGAATATGCTGTCATTCAGGTGCTGCATTGGTTTCGGCGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGTAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGACAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTTGAGTCGATCCCGTAAATCGTGGCCTTGCGTGCAAAGCTTTGCCGGACGGgaa                                                  <  1:354632/250‑1 (MQ=255)                                                                                                                                                                                                   gtcagtcaGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCAtt                                    <  2:465791/251‑1 (MQ=255)                                                                                                                                                                                                         cagGTGCTGCATTGGTTTCGACGTTTTGACGATTATAGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTg                                >  2:37880/1‑249 (MQ=255)                                                                                                                                                                                                             tgctgcATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCa                           >  2:159790/1‑250 (MQ=255)                                                                                                                                                                                                             tgctgcATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCa                           >  1:189904/1‑250 (MQ=255)                                                                                                                                                                                                              gctgcATTGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAAt                         <  1:6030/251‑1 (MQ=255)                                                                                                                                                                                                                     tGGTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCggg                   <  1:137141/250‑1 (MQ=255)                                                                                                                                                                                                                       gTTTCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGCGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTAt                >  1:335683/1‑251 (MQ=255)                                                                                                                                                                                                                          tCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTg              <  2:189904/250‑1 (MQ=255)                                                                                                                                                                                                                          tCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTg              <  2:183035/250‑1 (MQ=255)                                                                                                                                                                                                                          tCGACGTTTTGACGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTg              <  2:85938/250‑1 (MQ=255)                                                                                                                                                                                                                                     aCGATTATCGTATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGGAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTa  <  1:133251/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 CTGATTATGCTTTAGTCTGGCATCCTCCTGTTGAAATGCTGGCAGGGCGCGATCTTAAAGCGGTGTTCGCACTCGGGGCCGGTGTTGATTCTATTTTGAGCAAGCTACAGGCACACCCTGAAATGCTGAACCCTTCTGTTCCACTTTTTCGCCTGGAAGATACCGGTATGGGCGAGCAAATGCAGGAATATGCTGTCAGTCAGGTGCTGCATTGGTTTCGACGTTTTGACGATTATCGCATCCAGCAAAATAGTTCGCATTGGCAACCGCTGCCTGAATATCATCGGGAAGATTTTACCATCGGCATTTTGGGCGCAGGCGTACTGGGCAGTAAAGTTGCTCAGAGTCTGCAAACCTGGCGCTTTCCGCTGCGTTGCTGGAGTCGAACCCGTAAATCGTGGCCTGGCGTGCAAAGCTTTGCCGGACGGGAAGAACTGTCTGCATTTCTGAGCCAATGTCGGGTATTGATTAATTTGTTA  >  NC_000913/1098001‑1098479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑